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| 1 | Hyaluronic acid fragments evoke Kupffer cells via TLR4 signaling pathway显示文摘Kupffer cells, expressing toll-like receptor 4 (TLR4), play a central role in hepatic ischemia/reperfusion (I/R) injury. Hyaluronic acid (HA) fragments, degradative products of high-molecular-weight HA (HMW-HA), acquire the ability to activate immune cells under inflammatory conditions. Here we inves- tigated whether HA fragments could activate Kupffer cells and analyzed the underlying mechanism. Kupffer cells were isolated from wild-type mice (WT, C3H/HeN) and TLR4 mutant mice (C3H/HeJ) and HA fragments were produced by the methods of enzyme digestion and chromatography. Then Kupffer cells were stimulated by HA fragments or other control stimuli. The activation of Kupffer cells was estimated as the release of pro-inflammatory cytokines. The activation of p38 MAPK pathway of Kupffer cells was checked and blocking experiments were done as well. The results indicated that HA fragments acquired the ability to activate Kupffer cells in vitro, which was TLR4 dependent and not due to contamination of lipopolysaccharide. Experiments of p38 MAPK kinase inhibition by SB-203580 verified p38 MAPK was required in HA fragments induced Kupffer cells activation. This suggests that HA fragments, degradative products of one of the major glycosaminoglycans of the extracellular matrix, play critical roles in Kupffer cell activation mediated by TLR4 signaling pathway, which is, at least partially, de- pendent on p38 MAPK activation. | ZHANG JinXiang1, WANG Hui2, XIAO Qing3, LIANG HuiFang4, LI ZhuoYa4, JIANG ChunFang1, WU HeShui5 & ZHENG QiChang5 1 Department of Emergency Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Medical Genetics, Tongji Medical College Affiliated to Huazhong University of Science and Technology, Wuhan 430030, China 3 Department of Ophthalmology, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China 4 Department of Medical Immunology, Tongji Medical College Affiliated to Huazhong University of Science and Technology, Wuhan 430030, China 5 Department of General Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China | 2009 | Science China(Life Sciences)2009，52，2： | 33 |
| 2 | On the new results of global attractive set and positive invariant set of the Lorenz chaotic system and the applications to chaos control and synchronization显示文摘Constructing a family of generalized Lyapunov functions, a new method is proposed to obtain new global attractive set and positive invariant set of the Lorenz chaotic system. The method we proposed greatly simplifies the complex proofs of the two famous estimations presented by the Russian scholar Leonov. Our uniform formula can derive a series of the new estimations. Employing the idea of intersection in set theory, we extract a new Leonov formula-like estimation from the family of the estimations. With our method and the new estimation, one can confirm that there are no equilibrium, periodic solutions, almost periodic motions, wandering motions or other chaotic attractors outside the global attractive set. The Lorenz butterfly-like singular attractors are located in the global attractive set only. This result is applied to the chaos control and chaos synchronization. Some feedback control laws are obtained to guarantee that all the trajectories of the Lorenz systems track a periodic solution, or globally stabilize an unstable (or locally stable but not globally asymptotically stable) equilibrium. Further, some new global exponential chaos synchronization results are presented. Our new method and the new results are expected to be applied in real secure communication systems. | LIAO Xiaoxin 1, 2, 3 , FU Yuli 4 & XIE Shengli 4 1. Department of Control Science & Control Engineering, Huazhong University of Science & Technology, Wuhan 430074, China 2. School of Automation, Wuhan University of Science & Technology, Wuhan 430070, China 3. School of Information, Central South University of Economy, Politics and Law, Wuhan 430064, China 4. School of Electronics & Information Engineering, South China University of Technology, Guangzhou 510640, China Correspondence should be addressed to Liao Xiaoxin (email: xiaoxin_liao@hotmail.com) | 2005 | Science in China(Series F)2005，48，3： | 23 |
| 3 | GJB2 mutation spectrum in deaf population in a typical southeastern area of China显示文摘Mutations in GJB2 gene are the most frequently found mutations in patients with nonsyndromic hearing impairment. However, the spectrum and prevalence of mutations in this gene vary among different ethnic groups. In China, 30,000 infants are born with congenital hearing impairment annually. In order to provide appropriate genetic testing and counseling to the families, we investigated the molecular etiology of nonsyndromic deafness in 103 unrelated school children attending Nantong School for the Deaf and Mute in Jiangsu Province, China. The coding exon of the GJB2 gene was PCR amplified and sequenced. Sixty two GJB2 mutant alleles were identified in 35.9% (37/103) of the patients. Twenty five patients carried two pathogenic mutations and 12 patients carried one mutant allele. The 235delC was the most common mutation accounting for 69.4% (43/62) of GJB2 mutant alleles. The GJB2 mutant alleles accounted for 30.1% (62/206) of all chromosomes responsible for nonsyndromic hearing impairment. Testing of the 3 most prevalent deleterious frame shift mutations in this cohort detected 100% of all GJB2 mutant alleles. These results demonstrate that an effective genetic testing of GJB2 gene for patients and families with nonsyndromic hearing impairment is possible. | DAI Pu1*, YOU Yi-wen2*, CUI Jing-hong2*, YU Fei1, HAN Bing 1, KANG Dong-yang1, YUAN Hui-jun1, HAN Dong-yi1, 1. Department of Otolaryngology, PLA General Hospital, Beijing, People’s Republic China, 100853 2. Department of Otolaryngology, Nantong University Affiliated Hospital, Nantong, Jiangsu Province, People’s Republic China, 226001 *Pu Dai, Yiwen You, Jinghong Cui contribute equally to this paper | 2006 | Journal of Otology2006，1，2： | 10 |
| 4 | Proteomic analysis of mitochondria reveals a metabolic switch from fatty acid oxidation to glycolysis in the failing heart显示文摘This work characterizes the mitochondrial proteomic profile in the failing heart and elucidates the molecular basis of mitochondria in heart failure. Heart failure was induced in rats by myocardial infarction, and mitochondria were isolated from hearts by differential centrifugation. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry, a system biology approach was employed to investigate differences in mitochondrial proteins between normal and failing hearts. Mass spectrometry identified 27 proteins differentially expressed that involved in energy metabolism. Among those, the up-regulated proteins included tricarboxylic acid cycle enzymes and pyruvate dehydrogenase complex subunits while the down-regulated proteins were involved in fatty acid oxidation and the OXPHOS complex. These results suggest a substantial metabolic switch from free fatty acid oxidation to glycolysis in heart failure and provide molecular evidence for alterations in the structural and functional parameters of mitochondria that may contribute to cardiac dysfunction during ischemic injury. | WANG Jun1,2, BAI Ling1,2, LI Jing3, SUN ChaoFeng1,2, ZHAO Jin1, CUI ChangCong1,2, HAN Ke1,2, LIU Yu1,2, ZHUO XiaoZhen1,2, WANG TingZhong1,2, LIU Ping1,2, FAN FenLing1,2, GUAN YouFei3 & MA AiQun1,2 1 Department of Cardiology, First affiliated hospital of medical college of Xi’an Jiaotong University, Xi’an 710061, China 2 Ion Channel Disease Laboratory, Key Laboratory of Environment and Genes Related to Diseases of Education Ministry, Xi’an 710061, China 3 Department of Physiology and Pathophysiology Key Laboratory of Molecular Cardiovascular Sciences of the Ministry of Education, Peking University Health Science Center, Beijing 100083, China | 2009 | Science China(Life Sciences)2009，52，11： | 10 |
| 5 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 6 | A numerical investigation of the evolution of 2-D disturbances in hypersonic boundary layers and the effect on the flow structure due to the existence of shocklets显示文摘 | CAO Wei & ZHOU Heng Department of Mechanics,Tianjin University Liu Hui Center of Applied Mathematics,Nankai and Tianjin University,Tianjin 300072,China Correspondence should be addressed to Zhou Heng(email:hzhou 1 @ tju.edu.cn) | 2004 | Science China(Physics,Mechanics & Astronomy)2004，47，2： | 4 |
| 7 | Liver Nuclear Activation of Carbon Tetrachloride or Bromotrichloromethane to Trichloromethyl and Trichloromethylperoxyl Free Radicals.Their Reactions With Lipids and Proteins显示文摘The formation of·CCl3 radicals in liver nuclei was suggested by spin trapping of them with N-t-butyl-α-phenylnitrone followed by GC/MS detection of the resulting adduct. Comparison of its formation in microsomal biotransformation of CCl4 was made. In aerobic nuclear activation mixtures containing NADPH and CCl4, significant decrease in the arachidonic acid content of nuclear lipids was observed (27. 8%, compared to control), the intensity of this decrease was lower than that occurring in the corresponding microsomal incubation mixtures (29.1%). Significant decreases in arachidonic acid content of nuclear and endoplasmic reticulum lipids were also observed in animals at 6 hours of poisoning with the haloalkane. During aerobic nuclear metabolism of CCl4 or CBrCl3, cholesterol oxidation products were detected: a ketocholesterol, an epoxide like structure and 7-ketocholesterol. Nuclear protein carbonyl formation was not promoted during nuclear CCl4 biotransformation. NADPH by itself may lead to protein carbonyl formation during prolonged periods of incubation. CBrCl3 in contrast, led to decreased protein carbonyl formation. No increase in nuclear protein carbonyl formation was observed in CCl4 intoxicated animals during periods of time between 1 to 6 hours after treatment. The results indicate that during nuclear biotransformation of CCl4 or CBrCl3 reactive free radicals, PUFA degradation, reactive aldehydes and cholesterol oxidation products are formed, nearby DNA and regulatory proteins. | S. L. FANELLI G. D. CASTRO M. E. GALELLI AND J. A. CASTRO (Centro de Investigaciones Toxicologicas(CEITOX)-CITEFA/CONICET, Zufriategui 4380, 1603 Villa Martelli,Buenos Aires, Argentina)(Send correspondence to Dr. Jose Alberto Castro, Zufriategui 4380, 1 | 1998 | Biomedical and Environmental Sciences1998，11，2： | 4 |
| 8 | Split liver transplantation: a reliable approach to expand donor pool显示文摘Orthotopic liver transplantation as a successful treatment of end-stage liver disease is hampered by a persistent lack of cadaveric organs. Split liver transplantation, which was first successfully performed by Medical School of Hannover in 1988, has become a mature surgical technique to expand the donor pool. Between 1993 and 1999, split liver transplantation activities have increased in Europe from 1. 2% to 10. 4% in all performed liver transplantations. Current data have strongly supported that the survival rate of patients after split liver transplantation is not significantly different from that of patients after whole-size orthotopic liver transplantation. The most important step of donor graft selection is surgeon’ s observation judged by the experience of individual transplant center. The paper aims to provide the guideline of donor selection, hepatic graft splitting, and recipient management as well. DATA SOURCES: Medical School of Hannover has accumulated plentiful experience of split liver transplantation for more than 10 cases ever since 1998. Besides that, we also reviewed a variety of literatures from other famous European and American centers specialized in this field for many years. RESULTS:According to our experience combined with the view points of others, the donor should meet the following criteria as well: (1) age less than 50 years; (2) hemodynamics stable; (3) ICU less than 5 days; (4) Na less than 170 mmol/L or better if less than 150 mmol/L. In 1996 and 1997, the Hamburg group and the UCLA group separately introduced a breakthrough technique performing split liver transplantation in situ. Evidently, the in situ technique has been limited by prolonged time of donor organ procurement , coordination with other organ procurement teams, and even extra burden on donor hospital. Some groups, therefore, have restored the ex situ or bench splitting technique , and fortunately the transplant outcomes of the ex situ technique are equivalent to those of the in situ one. Recently some new techniques have been introduced to split the liver for two adult patients, including the split-cava technique. CONCLUSIONS:It is clear that the most important factor for determining the prognosis of the patient is the time of receiving liver transplantation, not the type of liver transplantation. We still need to pay close attention to the graft to recipient weight ratio (GRWR) and the UNOS classification or MELD score before the patient is subjected to split liver transplantation. | Ji-Qi Yan, Thomas Becker, Cheng-Hong Peng, Hong-Wei Li and Juergen Klempnauer Department of Surgery, Ruijin Hospital Affiliated to Shanghai Second Medical University, Shanghai 200025, China ( Yan JQ, Peng CH and Li HW) Department of Abdominal and Transplant Surgery, Medical School of Hannover, Carl-Neuberg-Str. 1 , 30625 Hannover, Germany | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 3 |
| 9 | A Convenient Synthesis of Chrysin and Tectochrysin显示文摘Chrysin(1) and tectochrysin(2) were respectively synthesized in four steps from 1,3,5-tri-bromobenzene in an overall yield of 46.8% and that of 37.0% with the key step being theBu4NBr catalyzed hydrolysis of 1-phenyl-3- ( 2 ', 4 ' ,6 ' -trimethoxyphenyl )-1,3-propanedione (11) under different conditions. | ZHU Jin-tao~(1,*), CHEN Ying-qi~2, DAI Li-yan~2, SUN Li-wen~1 and YAO San-jing~2(2. Department of Chemical Engineering, Zhejiang University, Hangzhou 310013, P. R. China 1. Zhejiang Engineering College, Hangzhou 310033, P. R. China) To whom correspondence should be addressed. | 2001 | Chemical Research in Chinese Universities2001，17，3： | 2 |
| 10 | Langmuir-Blodgett Films from Schiff Base Aluminium (Ⅲ) Complex显示文摘The characteristics of the LB films of Schiff base aluminium( Ⅲ ), tris(2-hydroxy-5-nitro N-dodecyl-benzylideneaminato) aluminium ( Ⅲ ) [Al (TA 12) 3], were studied. The surface pres sure-area(r-A) isotherm of Al(TA12)3 in the pure water subphase was investigated. The molec ular area, 0.48 nm2, is one-third of the expected value that indicates the formation of an aggre gate. The Langmuir-Blodgett (LB) films of Al(TA12) 3 were transferred and characterized. The UV-Vis spectra and the AFM image both confirmed that the J-aggregates formed. The polarized UV-Vis spectra indicated that the complex plane had to be oriented with an angle of about 30° to the substrate surface. The IR spectra suggested that the complexation took place between aluminium ions and the oxygen atoms of the ligand rather than the nitrogen atom. | LIAN Wei~1, CAO Wei~2, WANG Gang~2, WEI Li~2, HONG Xia~2, SHEN Dong-fang~2 LIU Chun-yan~1, SHAO Hui-bo~(3**) and BAI Yu-bai~2(1. Institute of Photographic Chemistry, Chinese Academy of Sciences, Beijing 100101, P. R. China 2. Center for Intelligent Materials United Research(CIMUR), Department of Chemistry, Jilin University, Changchun 130023, P. R. China 3. Department of Chemistry, Capital Normal University, Beijing 100037, P. R. China) To whom correspondence should be addressed. | 2001 | Chemical Research in Chinese Universities2001，17，3： | 2 |
| 11 | Murine fertilized ovum, blastomere and morula cells lacking SP phenotype显示文摘In the field of stem cell research, SP (side population) phenotype is used to define the property that cells maintain a high efflux capability for some fluorescent dye, such as Hoechst 33342. Recently, many researches proposed that SP phenotype is a phenotype shared by some stem cells and some pro- genitor cells, and that SP phenotype is regarded as a candidate purification marker for stem cells. In this research, murine fertilized ova (including conjugate and single nucleus fertilized ova), 2-cell stage and 8-cell stage blastomeres, morulas and blastocysts were isolated and directly stained by Hoechst 33342 dye. The results show that fertilized ovum, blastomere and morula cells do not demonstrate any ability to efflux the dye. However, the inner cell mass (ICM) cells of blastocyst exhibit SP phenotype, which is consistent with the result of embryonic stem cells (ESCs) in vitro. These results indicate that the SP phenotype of ICM-derived ESCs is an intrinsic property and independent of the culture condition in vitro, and that SP phenotype is one of the characteristics of at least some pluripotent stem cells, but is not shared by totipotent stem cells. In addition, the result that the SP phenotype of ICM cells disap- peared when the inhibitor verapamil was added into medium implies that the SP phenotype is directly associated with ABCG2. These results suggest that not all the stem cells demonstrate SP phenotype, and that SP phenotype might act as a purification marker for partial stem cells such as some pluripo- tent embryonic stem cells and multipotent adult stem cells, but not for all stem cells exampled by the totipotent stem cells in the very early stage of mouse embryos. | XU YiXin1, HE ZhiYing2, ZHU HaiYing2, CHEN XueSong2, LI JianXiu2, ZHANG HongXia2, PAN XingHua3 & HU YiPing2 1 ChangZheng Hospital, affiliated to Second Military Medical University, Shanghai 200003, China 2 Department of Cell Biology, Second Military Medical University, Shanghai 200433, China 3 Department of Genetics, Yale University School of Medicine, New Haven, CT 06519, USA | 2007 | Science China(Life Sciences)2007，50，6： | 2 |
| 12 | Infection rates following initial cerebrospinal fluid shunt placement across pediatric hospitals in the United States显示文摘 | Simon TO Hall M Riva-Cambrin 1 | 2009 | 1 Neurosurg Pediatr2009，4，2： | 1 |
| 13 | Platelet aggregates- genitor cell proliferation and and foam cells is mediated by in vitro 显示文摘 | Stellos K Seizer P Bigalke induced human CD34 + pro different stromal Hemost iation to macrophages cell derived factor 1 B | 2010 | Semin Thromb2010，36，2： | 1 |
| 14 | Basic Concepts显示文摘 | Robust Statistics-How Not to Reject Outliers Part 1 | 1989 | Analyst1989，，114： | 1 |
| 15 | 显示文摘 | Dijken A Schluepmann H Smeekens S Arabidopsis Treha- lose - 6 - Phosphate Synthase 1 Is Essential for Normal Vegetative Growth and Transition to Flowering | 2004 | Plant Physiology2004，135，： | 1 |
| 16 | 2-q13 显示文摘 | Higashimoto I Yoshiura K Hirakawa N et aL Primary palmar hy- perhidrosis locus maps to 14ql 1 | 2006 | Am J Med Genet A2006，140，6： | 1 |
| 17 | Dev 显示文摘 | Mizoguchi T Wheatley K Hanzawa Y et aL LHY and CCA 1 are partially redundant genes required to maintain circadian rhythms in Arabidopsis | 2002 | Cell2002，10，： | 1 |
| 18 | berghei mouse model 显示文摘 | Merkli B Rinchle 1 Study on the resistance to single and combined antimalarias in the P | 1980 | Acta Trop1980，37，： | 1 |
| 19 | ATF3 inhibits doxorubicin-induced apoptosis in cardiac myocytes : a novel cardioprotective role of ATF3 显示文摘 | Nobori K 1 to H Tamamori-Adachi M | 2002 | J Mol Cell Cardiol2002，34，： | 1 |
| 20 | Immunosuppression in patients who die of sepsis and multiple organ failure显示文摘 | Boomer 1 S To K Chang K C | 2011 | lAMA2011，306，23： | 1 |
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