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| 1 | Microscopy Observations of Habitable Space in Biochar for Colonization by Fungal Hyphae From Soil显示文摘Biochar is a potential micro-environment for soil microorganisms but evidence to support this suggestion is limited. We explored imaging techniques to visualize and quantify fungal colonization of habitable spaces in a biochar made from a woody feedstock. In addition to characterization of the biochar, it was necessary to optimize preparation and observation methodologies for examining fungal colonization of the biochar. Biochar surfaces and pores were investigated using several microscopy techniques. Biochar particles were compared in soilless media and after deposition in soil. Scanning electron microscopy(SEM) observations and characterization of the biochar demonstrated structural heterogeneity within and among biochar particles. Fungal colonization in and on biochar particles was observed using light, fluorescence and electron microscopy. Fluorescent brightener RR 2200 was more effective than Calcofluor White as a hyphal stain. Biochar retrieved from soil and observed using fluorescence microscopy exhibited distinct hyphal networks on external biochar surfaces. The extent of hyphal colonization of biochar incubated in soil was much less than for biochar artificially inoculated with fungi in a soilless medium. The location of fungal hyphae was more clearly visible using SEM than with fluorescence microscopy. Observations of biochar particles colonized by hyphae from soil posed a range of difficulties including obstruction by the presence of soil particles on biochar surfaces and inside pores. Extensive hyphal colonization of the surface of the biochar in the soilless medium contrasted with limited hyphal colonization of pores within the biochar. Both visualization and quantification of hyphal colonization of surfaces and pores of biochar were restricted by two-dimensional imaging associated with uneven biochar surfaces and variable biochar pore structure. There was very little colonization of biochar from hyphae in the agricultural soil used in this study. | Noraini M Jaafar Peta L Clode Lynette K Abbott | 2014 | Journal of Integrative Agriculture2014，13，3： | 10 |
| 2 | Nat Genet:单基因突变导致过敏性皮炎的发生显示文摘最近,研究者们鉴定出了一类导致神经性皮炎发生的关键基因突变:CARDll。来自美国NIH过敏与传染病研究所的研究者们通过对四个没有血缘关系的患病家庭进行分析,发现了这一导致疾病产生的基因、 | Chi A Ma, Yuan Zhang, Michael A Weinreich, Jonathan J Lyons, Celeste G Nelson, Thomas DiMaggio, Kelly D Stone, Joshua D Milner Jeffrey R Stinson, Elisa Ruffo, Batsukh Dorjbal, Swadhinya Arjunaraja, Kelsey Voss, Andrew L Snow Jordan K Abbott, Pia J Hauk, Paul R Reynolds, Erwin W Gelfand Elisa Ruffo Salomé Glauzy, Natsuko Yamakawa, Eric Meffre Jennifer Stoddard, Julie Niemela, Sergio D Rosenzweig Yu Zhang, Helen F Matthews Joshua J McElwee Nina Jones Alejandro Palma, Matías Oleastro, Emma Prieto, Andrea R Bernasconi, Geronimo Dubra, Silvia Danielian, Jonathan Zaiat, Marcelo A Marti Brian Kim Megan A Cooper Neil Romberg | 2017 | 现代生物医学进展2017，17，27： | 3 |
| 3 | Principles for Measurement of Chemical Expo- sure based on Recognition - Driven Anchoring Transitions in Liquid Crystals 显示文摘 | Shah R R Abbott N L | 2001 | Science2001，293，： | 1 |
| 4 | Lattice vi- brations of the icosahedral solid boron arsenide, B12As2显示文摘 | Beckel C L Lu N Abbott B | 1999 | Inorganica Chimica Aeta1999，289，12： | 1 |
| 5 | Influence of molecular level interaction on the orientations of liquid crystals supported on nanostructured surfaces presenting specifically bound proteins显示文摘 | Skaife J J Abbott N L | 2001 | Langmuir2001，17，18： | 1 |
| 6 | The effect of phosphorus on the formation of hyphae in soil by vesicular mycorrhizal fungus ,Glomus fasciculatum显示文摘 | Abbott L K Robson A D Boer G | 1984 | New phytologist1984，97，： | 1 |
| 7 | Arteriovenous ?s-tula use and heart disease in long term elderly hemo?dialysis p-atients: analysis of United States Renal Data System Dialysis Morbidity and Mortality Wave II显示文摘 | Abbott K Trespalacios F Agodoa L | 2003 | J Nephro12003，16，： | 1 |
| 8 | Efficient patient identification strategies for women with osteoporosis显示文摘 | Abbott TA 3rd Mucha L Manfredonia D | | 0，，03： | 1 |
| 9 | Case of Aeromonas veronii( DNA Group 10 ) bacteremia 显示文摘 | ABBOTT S L SERVE H JANDA J M | 1994 | J Clin Microbiol1994，32，12： | 1 |
| 10 | Multicenter evaluation of the Microscan rapid gram-negative identification type 3 panel显示文摘 | Bascomb S Abbott S L | 1997 | Journal of Clinical Microbiology1997，35，10： | 1 |
| 11 | Deacylation of echinocandin B by Actinoplanes utahensis显示文摘 | Boeck L D Fukuda D S Abbott B J | 1989 | J Antibiot (Tokyo)1989，42，3： | 1 |
| 12 | Inhibition of hyphal growth of a vesicular-arbuscular mycorrhizal fungus in soil containing sodium chloride limits the spread of infection from spores 显示文摘 | MCMILLEN B G JUNIPER S ABBOTT L K | 1998 | Soil Biology and Biochemistry1998，30，13： | 1 |
| 13 | MiRPl forms IKr potassiumchannels with HERG and is associated with cardiac arrhythmia显示文摘 | Abbott GW’Sesti F Splawski l | 1999 | Cell1999，97，2： | 1 |
| 14 | C-reactive protein and the future risk of thromboembolic stroke in healthy men显示文摘 | Curb J D Abbott R D Rodriguez B L | 2003 | Circulation2003，107，： | 1 |
| 15 | Multiple myeloma and light chain-associated nephropathy at end-stage renal disease in the United States:patient characteristics and survival显示文摘 | ABBOTT K C AGODOA L Y | 2001 | Clin Nephrol2001，56，3： | 1 |
| 16 | Manipulation of the orientational response of liquid crystals to proteins specifically bound to covalently immobilized and mechanically sheared films of functionalized bovine serum albumin显示文摘 | Kim S R Abbott N L | 2002 | Langmuir2002，18，13： | 1 |
| 17 | Relationship of sonic resonant frequency to compression tests and Magness-Taylor firmness of apples during refrigerated storage显示文摘 | Abbott J A Liljedahl L A | 1994 | Transactions of the ASAE1994，37，4： | 1 |
| 18 | Control system design for VSC transmission 显示文摘 | Jovcic D Lamont L Abbott K | 2007 | Electric Power Systems Research2007，77，7： | 1 |
| 19 | Astrocyte-endothelial interactions at the blood-brain barrier显示文摘 | Abbott N J Ronnback L Hansson E | 2006 | Nat Rev Neurosci2006，7，： | 1 |
| 20 | Deacylation of echinocandin B by Actinoplanes utahensis显示文摘 | BOECK L D FUKUDA D S ABBOTT B J | 1989 | J Antibiot1989，42，3： | 1 |
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