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| 1 | IL28B polymorphism and cytomegalovirus predict response to treatment in Egyptian HCV type 4 patients显示文摘AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers. | Mostafa K El Awady Noha G Bader El Din Ashraf Tabll Yaser El Hosary Ashraf O Abdel Aziz Hesham El Khayat Mohsen Salama Tawfeek H Abdelhafez | 2013 | World Journal of Gastroenterology2013，19，2： | 8 |
| 2 | Prevalence and risk factors of asymptomatic hepatitis C virus infection in Egyptian children显示文摘AIM: To identify the prevalence, risk factors and manifestations of asymptomatic hepatitis C virus (HCV) infection in Egyptian children. METHODS: Children at the age of 1-9 years were screened for HCV antibodies and alanine aminotransferase (ALT) levels. Every child with elevated ALT and/or detectable HCV antibodies was tested for HCV RNA by RT-PCR and compared with two negative controls for risk factors and signs and symptoms of liver disease.RESULTS: We screened 1042 children, six of them had elevated ALT, negative HCV antibody and positive RNA, likely representing acute hepatitis C cases. Fifteen children were HCV seropositive, 5 of them were HCV RNA positive. Asymptomatic HCV infection was present in 2.02% (positive results for either HCV antibodies or HCV-RNA or both). Symptoms such as diarrhea, abdominal pain, history of fatigue and school absence because of illness and risk factors such as dental care were significantly more common among HCV positive cases than among controls. None of the HCV positive children was diagnosed as having signs of advanced liver disease upon clinical or ultrasonographic examination. CONCLUSION: Asymptomatic HCV infection is detectable in 2.02% Egyptian children. | MS El-Raziky M El-Hawary G Esmat AM Abouzied N El-Koofy N Mohsen S Mansour A Shaheen M Abdel Hamid H El-Karaksy | 2007 | World Journal of Gastroenterology2007，13，12： | 4 |
| 3 | The effects of saline irrigation water management anal salt tomato varieties on sustainable production of tomato in Syria (1999-2002) 显示文摘 | Abdel Gawad G Arslan A Gaihbe A | 2005 | Agricultural Water Management2005，78，12： | 1 |
| 4 | The effects of saline irrigation water management and salt tomato varieties on sustainable production of tomato in Syria(1999-2000)显示文摘 | G Abdel Gawad A Arslan A Gaihbe | 2005 | Agricultural Water Management2005，78，： | 1 |
| 5 | Fac- tors associated with quality of life of outpatients with breast cancer and gy- necologic cancers and their family care- givers: A controlled study显示文摘 | Abdel WA Jude UO Abdullah G | 2007 | BMC Cancer2007，13，7： | 1 |
| 6 | Salinity-calciumn interactions on root growth and osmotic adjustment of two corn cultivars differing in salt tolerance显示文摘 | Cramer G R Abdel B R Seeman J R | 1990 | Joumal of Plant Nutrition1990，13，： | 1 |
| 7 | Copper and chromium (Ⅲ) uptake by duckweed显示文摘 | Lubberding H J Alaerts G J | 1995 | Water Sci Technol1995，32，11： | 1 |
| 8 | Dynamic Single-Unit Representation of Induction Motor Groups显示文摘 | Abdel Hakim M M Berg G J | 1976 | IEEE Trans on PAS1976，95，1： | 1 |
| 9 | Tissue plasminogen activator and plasminogen activator inhibitor-1 levels in patients with acute myocardial infarction andunstable angina显示文摘 | Habib S Abdel G A Kurdi M I | 2012 | J Pak Med Assoc2012，62，7： | 1 |
| 10 | Cohesion intercept in effective stress- stability analysis显示文摘 | Mesri G Abdel G | 1993 | Journal of Geotechnical Engineering1993，119，8： | 1 |
| 11 | 显示文摘 | Abdel Malik T G Abdel-Latif R M | 1997 | Thin Solid Films1997，305，： | 1 |
| 12 | Microwave assisted synthesis and antimicrobial activities of flavonoid derivatives 显示文摘 | Abdel G S B Weaver L Zidan Z H | 2008 | Bioorg Med Chem Lett2008，18，2： | 1 |
| 13 | Gender determination from hand bones length and volume using multidetector compu- ted tomography~ A study in Egyptian people显示文摘 | Eshak G A Ahmed H M Gawad Abdel E A M | 2011 | Journal of Forensic and Legal Medicine2011，18，： | 1 |
| 14 | Akutes H?moperitoneum bei einer spontanen Tumorruptur als Erstmanifestation eines hepatozellul?ren Karzinoms显示文摘 | A Abdel Samie G Otto L Theilmann | 2007 | Z Gastroenterol2007，，07： | 1 |
| 15 | On a Graph-Theoretic Construction of Low Density Parity Check Codes 显示文摘 | DJURDJEVIC I LIN S ABDEL G K | 2003 | IEEE Commun Lett2003，，7： | 1 |
| 16 | Study of thesurface modification of LiNil/3Cocathode material forlithium ion battery 显示文摘 | HAS HEM A M A ABDEL G A E EID A E | 2011 | Journal of Power Sources2011，196，20： | 1 |
| 17 | New developments in stratigraphy and petroleum-geological evolution显示文摘 | Bunter M A G Abdel M A E M The Sudanese Red Sea:1 | 1989 | Journal of Petroleum Geology1989，12，2： | 1 |
| 18 | Factors associated with quality of life of outpatients with breast cancer and gynecologic cancers and their family caregivers:a controlled study显示文摘 | Abdel W Jude U Abdullah G | 2007 | BMC Cancer2007，19，7： | 1 |
| 19 | Effect of admixtures on the electrical behaviour of portland cement 显示文摘 | ABDEL W M G HELMY I M EL DIDAMONY H | 1993 | J Mater Sci Lett1993，12，1： | 1 |
| 20 | Sirolimus changes lipid concentrations and lipoprotein metabolism in kidney transplant recipients显示文摘 | MORRISETT J D ABDEL FATTAH G KAHAN B D | 2003 | Transplant Proc2003，35，3： | 1 |
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