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| 1 | hsa-miR-29c and hsa-miR-135b differential expression aspotential biomarker of gastric carcinogenesis显示文摘AIM: To investigate the expression profiles of hsa-mi R-29 c and hsa-mi R-135 b in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. METHODS: The expression levels of hsa-mi R-29 c and hsa-mi R-135 b in normal gastric mucosa, non-atrophic chronic gastritis, intestinal metaplasia and intestinaltype gastric adenocarcinoma were analysed using quantitative real-time PCR. The difference between hsa-mi R-29 c and hsa-mi R-135 b expression profiles in the grouped samples was evaluated by ANOVA and Student's t-test tests. The results were adjusted for multiple testing by using Bonferroni's correction. P values ≤ 0.05 were considered statistically significant. To evaluate hsa-mi R-29 c and hsa-mi R-135 b expressions as potential biomarkers of gastric carcinogenesis, we performed a receiver operating characteristic curve analysis and the derived area under the curve, and a Categorical Principal Components Analysis. In silico identification of the genetic targets of hsa-mi R-29 c and hsa-mi R-135 b was performed using different prediction tools, in order to identify possible genes involved in gastric carcinogenesis.RESULTS: The expression levels of hsa-mi R-29 c were higher in normal gastric mucosal samples, and decreased progressively in non-atrophic chronic gastritis samples, intestinal metaplasia samples and intestinal-type gastric adenocarcinoma samples. The expression of hsa-mi R-29 c in the gastric lesions showed that non-atrophic gastritis have an intermediate profile to gastric normal mucosa and intestinal-type gastric adenocarcinoma, and that intestinal metaplasia samples presented an expression pattern similar to that in intestinal-type gastric adenocarcinoma. This micro RNA(mi RNA) has a good discriminatory accuracy between normal gastric samples and(1) intestinal-type gastric adenocarcinoma; and(2) intestinal metaplasia, and regulates the DMNT3 A oncogene. hsa-mi R-135 b is up-regulated in non-atrophic chronic gastritis and intestinal metaplasia samples and down-regulated in normal gastric mucosa and intestinal-type gastric adenocarcinoma samples. Non-atrophic chronic gastritis and intestinal metaplasia are significantly different from normal gastric mucosa samples. hsa-mi R-135 b expression presented a greater discriminatory accuracy between normal samples and gastric lesions. This mi RNA was associated with Helicobacter pylori presence in non-atrophic chronic gastritis samples and regulates the APC and KLF4 tumour suppressor genes.CONCLUSION: Our results provide evidence of epigenetic alterations in non-atrophic chronic gastritis and intestinal metaplasia and suggest that hsa-mi R-29 c and hsa-mi R-135 b are promising biomarkers of gastric carcinogenesis. | Amanda Ferreira Vidal Aline MP Cruz Leandro Magalhães Adenilson L Pereira Ana KM Anaissi Nélisson CF Alves Paulo JBS Albuquerque Rommel MR Burbano Samia Demachki Ândrea Ribeiro-dos-Santos | 2016 | World Journal of Gastroenterology2016，22，6： | 7 |
| 2 | Cognitive functions in pa- tients with panic disorder: a literature review显示文摘 | Alves MR Pereira VM Machado S | 2013 | Rev Bras Psiquiatr2013，35，2： | 1 |
| 3 | Montreal Cognitive Assessment(MoCA):normative study for the portuguese population显示文摘 | Freitas S Simes MR Alves L | 2011 | Clin Exp Neuropsychol2011，33，9： | 1 |
| 4 | Single intraoperative application verus postoperative mitomycin C eye drops in pterygium surgery显示文摘 | Cardill JA Alves MR Ambrosio LE | 1995 | Ophthalmology1995，102，： | 1 |
| 5 | Autoantibodies and high-risk HLA susceptibility markers in first-degree rela- tives of Brazilian patients with type 1 diabetes mellitus:a progression to disease based study显示文摘 | Alves LI Davini E Correia MR | 2012 | Clin Immunol2012，32，4： | 1 |
| 6 | Mannose-binding lectin gene polymorphism and its impact on human immunodeficiency virus 1 infection显示文摘 | Menezes-Costa MR Alves AEM | 2006 | Mol Immunol2006，43，9： | 1 |
| 7 | Montreal cognitive assessment(MoCA):validation study for vascular dementia显示文摘 | Freitas S Simoes MR Alves L | 2012 | J Int Neuropsychol Soc2012，18，6： | 1 |
| 8 | Evaluation of IL17A expression and of IL17A,IL17F and IL23R gene polymorphisms in Brazilian individuals with periodontitis显示文摘 | Saraiva AM Alves e Silva MR Correia Silva Jde F | 2013 | Hum Immunol2013，74，2： | 1 |
| 9 | Elecavnic and Magnetic Properties of SnO2/CrO2 Thin Super]attices显示文摘 | Pablo D Borges Lulsa MR Scolfaro Horcio W Leite Alves at al | 2011 | Nanoscale Research Letters2011，6，： | 1 |
| 10 | Autoantibodies and high-risk HLA susceptibility markers in first-degree relatives of Brazilian patients with type 1 diabetes mellitus:a progression to disease based study显示文摘 | Alves LI Davini E Correia MR | 2012 | Journal of Clinical Immunology2012，32，4： | 1 |
| 11 | Prevalence of diarrheagenic Escherichia coil in children with diarrhea in Salvador, Bahia, Brazil 显示文摘 | Franzolin MR Alves RC Keller R | 2005 | Mere Inst Oswaldo Cruz2005，100，4： | 1 |
| 12 | Evaluation of a new system for obtaining donor lamellar grafts 显示文摘 | Victor G Sousa SJ Alves MR Nose W | 2007 | Cornea2007，26，2： | 1 |
| 13 | Estrogenic regulation of testicular expression of stem cell factor and c-kit: implications in germ cell survival and male fertility显示文摘 | Correia S Alves MR Cavaeo JE | 2014 | Fertil Steril2014，102，1： | 1 |
| 14 | Montreal Cognitive Assessment (MoCA):validation study for frontotemporal dementia显示文摘 | Freitas S Sim(o)es MR Alves L | 2012 | J Geriatr Psychiatry Neurol2012，25，： | 1 |
| 15 | Simplified versus traditional techniques for complete denture fabrication:A systematic review显示文摘 | Paulino MR Alves LR Gurgel BG | 2015 | J Prosthet Dent2015，113，1： | 1 |
| 16 | In vivo confocal microscopy in the diagnosis of fungal keratitis:case report显示文摘 | Victor G Alves MR Nose W | 2006 | Arq Bras Oftalmol2006，68，3： | 1 |
| 17 | Mortality due to cancer of the u- terine cervix in the state of Minas Gerais, Brazil, 1980-2005 :period and cohort analysis 显示文摘 | Alves CM Bastos RR Guerra MR | 2010 | Cad Saude Publica2010，26，7： | 1 |
| 18 | Montreal Cognitive Assessment (MoCA):validation study for vascular dementia显示文摘 | Freitas S Sim6es MR Alves L | 2012 | Int Neuropsychol Soc2012，18，： | 1 |
| 19 | Montreal Cognitive Assess- ment (MoCA) : validation study for vascular dementia 显示文摘 | Freitas S Similes MR Alves L | 2012 | J Int Neuropsychol Soc2012，18，6： | 1 |
| 20 | Free and conjugated biogenic amines in green and roasted coffee beans 显示文摘 | CASAL S MENDES E ALVES MR | 2004 | Journal of Agricultural and Food Chemistry2004，，52： | 1 |
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