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| 1 | 犬粪便棘球绦虫抗原的双抗体夹心酶联免疫吸附试验检测法建立与应用显示文摘目的研究双抗体夹心酶联免疫吸附检测犬粪便棘球绦虫虫体抗原的方法并评价其在实际工作中的应用价值。方法实验犬12只,分实验组和对照组,实验组用活化绵羊源原头蚴(G1型)作人工感染;感染后0~8周内收集粪便,56d宰杀实验犬,收集成虫;制备成虫抗原和虫体代谢/分泌抗原,蛋白酶K处理抗原并分别免疫成年绵羊和新西兰白兔;绵羊抗血清用80%饱和硫酸铵沉淀、透析,兔抗血清过IgG亲和层析柱,分别获得纯化抗体;观察犬粪便在PBS液中-80℃冷冻3d或在含1%Formalin的PBS液中70℃~80℃加热8h的处理方法对检出结果的影响。运用该方法对四川省甘孜县达通玛区4个乡的580只家犬吡喹酮每6月一次驱虫前后的粪抗原进行监测。结果试验系统的敏感性为92.3%,特异性为80%;粪抗原从感染后的第2~3周可检出;两种样品处理方法都可以灭活虫卵但不影响检出效果;检测方法可同时用于对细粒棘球绦虫和多房棘球绦虫感染的诊断;达通玛区家犬粪抗原阳性率从2000年的50%下降到2005年的17%。结论检测方法具有较高的特异性和敏感性;推荐粪便用加热的方法处理可以灭活虫卵以防止生物污染;该方法可以在基层推广应用。 | 黄燕 Heath D David Antone J Christine 邱加闽 王谦 | 2008 | 预防医学情报杂志2008，24，6： | 22 |
| 2 | On tolerability and safety of a maintenance treatment with 6-thioguanine in azathioprineor 6-mercaptopurine intolerant IBD patients显示文摘AIM: To determine the tolerability and safety profile of a low-dose maintenance therapy with 6-TG in azathioprine (AZA) or 6-mercaptopurine (6-MP) intolerant inflammatory bowel disease (IBD) patients over a treatment period of at least 1 year.METHODS: Database analysis.RESULTS: Twenty out of ninety-five (21%) patients discontinued 6-TG (mean dose 24.6 mg; mean 6-TGN level 540 pmol/8×108 RBC) within 1 year. Reasons for discontinuation were GI complaints (31%), malaise (15%)and hepatotoxicity (15%). Hematological events occurred in three patients, one discontinued treatment. In the 6-TG-tolerant group, 9% (7/75) could be classified as hepatotoxicity. An abdominal ultrasound was performed in 54% of patients, one patient had splenomegaly.CONCLUSION: The majority of AZA or 6-MP-intolerant IBD patients (79%) is able to tolerate maintenance treatment with 6-TG (dosages between 0.3 and 0.4 mg/kg per d). 6-TG may still be considered as an escape maintenance immunosuppressant in this difficult to treat group of patients, taking into account potential toxicity and efficacy of other alternatives. The recently reported hepatotoxicity is worrisome and 6-TG should therefore be administered only in prospective trials. | Nanne KH de Boer Luc JJ Derijks Lennard PL Gilissen Daniel W Hommes Leopold GJB Engels Sybrand Y de Boer Gijsbertus den Hartog Piet M Hooymans Anja BU M(?)kelburg Barend D Westerveld Anton HJ Naber Chris JJ Mulder Dirk J de Jong | 2005 | World Journal of Gastroenterology2005，11，35： | 4 |
| 3 | The presence of ANP in rat peritoneal mast cells显示文摘Atrial natriuretic peptide (ANP) is an important component of the natriuretic peptide system. A great role in many regulatory systems is played by mast cells. Meanwhile involvement of these cells in ANP activity is poorly studied. In this work, we have shown the presence of ANP in rat peritoneal mast cells. Pure fraction of mast cells was obtained by separation of rat peritoneal cells on a Percoll density gradient. By Western blotting, two ANP-immunoreactive proteins of molecular masses of 2.5 kDa and 16.9 kDa were detected in lysates from these mast cells. Electron microscope immunogold labeling has revealed the presence of ANP-immunoreactive material in storage, secreting and released granules of mast cells. Our findings indicate the rat peritoneal mast cells to contain both ANP prohormone and ANP. These both peptides are located in mast cell secretory granules and released by mechanism of degranulation. It is discussed that many mast cell functions might be due to production of natriuretic peptides by these cells. | Marina G MARTYNOVA Olga A BYSTROVA Olga M MOISEEVA Anton L EVDONIN Kirill A KONDRATOV Natalja D MEDVEDEVA | 2005 | Cell Research2005，15，10： | 3 |
| 4 | 基于石墨烯的光学控制窄带太赫兹开关(英文)显示文摘本文提出了一种光控太赫兹开关,该开关采用覆盖单层石墨烯的十字金属谐振器超表面。利用石墨烯表面电导率模型和有限元法计算了这种复合结构的光谱特性。模拟结果表明,在0.2 W/mm^2的光泵浦后,传输谱(调制深度为36.8%,Q-因子为250)出现了窄带共振衰减现象。另外,这种衰减的调制深度可以通过改变泵浦强度微调节。因此,光学可调谐太赫兹开关的设计将有助于太赫兹通信应用的功能组件开发。 | GREBENCHUKOV Alexander N ZAITSEV Anton D KHODZITSKY Mikhail K | 2018 | 中国光学2018，11，2： | 2 |
| 5 | Evaluation of refractory intermetallics with A15 structure for high temperature structural applications显示文摘 | Anton D L | 1992 | Mater Sci Eng A1992，153，： | 1 |
| 6 | Responses of forest herbs to available undemtory light measured with henrispherical photographs in silver fir-beech forest in Croatia显示文摘 | Oleg Antonic MaRio Bozic | 2006 | Ecological modelline2006，194，： | 1 |
| 7 | Preparation and surface properties of acrylic polymers containing fluorinated monomers 显示文摘 | Richard R Thomas D R Anton W F | 1997 | Macromolecules1997，30，： | 1 |
| 8 | Rising Incidence of Inflammatory Bowel Disease Among Children: A 12-year Study显示文摘 | Hoda M Malaty Xiaolin Fan Antone R Opekun Carolyn Thibodeaux George D Ferry | 2010 | Journal of Pediatric Gastroenterology and Nutrition2010，，： | 1 |
| 9 | Appraisal of other silicides as structural materials显示文摘 | Berczik D Anton D L | 1992 | Mater Sci Eng A1992，155，： | 1 |
| 10 | Chiral polymer catalysts in preparative organic chemistry:a critical overview显示文摘 | Alietto M Chiellini E Antone S D Ruggeri G Solaro R | 1988 | Pure Appl Chem1988，60，： | 1 |
| 11 | High temperature evaluation of topologically close packed intermetallics显示文摘 | Anton D L Shah D M | 1992 | Materials Science and Engineering A1992，153，12： | 1 |
| 12 | Behavioural despair in rats:a new model sensitive to antidepressant treatmeats显示文摘 | Porsolt R D Anton G Blavet N | 1978 | Eur J Pharmacol1978，47，4： | 1 |
| 13 | Hydrogen desorption kinetics in transition metal modified NaAIH4 显示文摘 | ANTON D L | 2003 | J Alloys Compd2003，，： | 1 |
| 14 | HydrometaUurgy 显示文摘 | Seggiani M Vitolo S Antone S D' | 2006 | 81(1): 9-142006，81，1： | 1 |
| 15 | Ultrastructural and cytochemical changes in cuhured human lung cells显示文摘 | Brandes D Murphy D G Anton E B et ol | 1972 | J Ultrastruct Res1972，39，5： | 1 |
| 16 | Cloning and characterization of the Bg/Ⅱ restriction-modification system reveals a possible evolutionary footprint显示文摘 | ANTON B P HEITER D F BENNER J S | 1997 | Gene1997，187，1： | 1 |
| 17 | Brain regions involved in the recognition of happiness and sadness in music显示文摘 | Khalfa S Schon D Anton JL | 2005 | Neuroreport2005，16，18： | 1 |
| 18 | Participatory and persuasive tele-health显示文摘 | Lee D? Helal S Anton S | 2012 | Gerontology2012，58，3： | 1 |
| 19 | Hydrogen desorption kinetics in transition metal modified NaAlH4 显示文摘 | ANTON D L | 2003 | J Alloys Compd2003，，： | 1 |
| 20 | The rheologieal behavior of some binders and plastics used in injection molding显示文摘 | NOVAC O A ANTON D NOVAC T | 1994 | Powder Metallurgy1994，94，： | 1 |
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