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| 1 | Hydrogen sutfide protects colon cancer cells from chemopreventative agent β-phenylethyl isothiocvanate induced apoptosis显示文摘AIM: Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI tract of mice and humans. Considering such high levels we speculate that, at non-toxic concentrations, H2S may interact with chemical agents and alter the response of colonic epithelium cells to such compounds. The GI tract is a major site for the absorption of phytochemical constituents such as isothiocyanates, flavonoids, and carotenoids, with each group having a role in the prevention of human diseases such as colon cancer. The chemopreventative properties of the phytochemical agent β-phenyethyl isothiocyanate (PEITC) are well recognized. However, little is currently known about the physiological or biochemical factors present in the GI tract that may influence the biological properties of ITCs. The current study was undertaken to determine the effects of H2S on PEITC mediated apoptosis in colon cancer cells.METHODS: Induction of apoptosis by PEITC in human colon cancer HCT116 cells was assessed using classic apoptotic markers namely SubG1 population analysis,caspase-3 like activity and nuclear fragmentation and condensation coupled with the MTT (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyl tetrasodium bromide) viability assay and LDH leakage.RESULTS: PEITC significantly induced apoptosis in HGT116cells as assessed by SubG1 population formation, nuclear condensation, LDH leakage and caspase-3 activity after 24 h, these data being significant from control groups (P<0.01). In contrast, co-treatment of cells with physiological concentrations of H2S (0.1-1 mmol/L) prevented PEITC mediated apoptosis as assessed using the parameters described.CONCLUSION: PEITC effectively induced cell death in the human adenocarcinoma cell line HCT116 in vitro through classic apoptotic mechanisms. However, in the presence of H2S, apoptosis was abolished. These data suggest that H2S may play a significant role in the response of colonic epithelial cells to beneficial as well as toxic agents present within the GI tract. | Peter Rose Philip K Moore Shen Han Ming Ong Choon Nam Jeffrey S Armstrong Matt Whiteman | 2005 | World Journal of Gastroenterology2005，11，26： | 13 |
| 2 | Phylogenetic relationships among Bactrocera species ( Diptera : Tephritidae) inferred from mitochondrial DNA sequences 显示文摘 | Smith P T Kambhanti S Armstrong K A | 2003 | Mor Phylogenet and Evol2003，26，： | 1 |
| 3 | Detection of anti body to the foot-and-mouth disease virus (FMDV) non-structural polyprotein 3ABC in sheep by ELISA显示文摘 | Armstrong R M Cox S J Aggarwal N | 2005 | J Virol Meth2005，125，2： | 1 |
| 4 | Statistical algorithm for nonuniformity correction in focalplane arrays显示文摘 | TORRES S ARMSTRONG E E | 1999 | Applied Optics1999，38，2： | 1 |
| 5 | Statistical algorithm for nonuniformity correction in focal-plane arrays显示文摘 | Hayat M M Torres S N Armstrong E E | 1999 | Applied Optics1999，38，： | 1 |
| 6 | The D-domain of Sp3modulates its protein levels and activation of the p21CIP1/WAF1promoter显示文摘 | Hammill D Jain N Armstrong S | 2005 | Biochem Biophys Res Commun2005，335，： | 1 |
| 7 | Cytokinin acitivity of Nphenyl-1,2,3-thidiazol-5-ylurea (TDZ)显示文摘 | Mok M C Mok D W S Armstrong D J | 1982 | Phytochemistry1982，21，： | 1 |
| 8 | Bovine congenital erythrocytic protoporphyria in a Limousin calf bred in the UK 显示文摘 | Armstrong S C Jonsson N N Barrett D C | 2002 | Vet Rec2002，19，： | 1 |
| 9 | Impaired nerve regeneration and enhanced neuroinflammatory response in mice lacking pituitary adenylyl cyclase activating peptide显示文摘 | Armstrong BD Abad C Chhith S Cheung-Lau G Hajji OE Nobuta H | 2008 | Neuroscience2008，151，1： | 1 |
| 10 | Usefulness of the par-alens fluorescent microscope adaptor for the identification of my-cobacteria in both field and laboratory settings 显示文摘 | Kuhn W Armstrong D Atteberry S | 2010 | Open Microbi-olj2010，4，4： | 1 |
| 11 | Inflammatory biomarkers in acute coronary syndromes : part II :acutephase reactants and biomarkers of endothelial cellactivation显示文摘 | ARMSTRONG E J MORROW D A SABATINE M S | 2006 | Circulation2006，113，7： | 1 |
| 12 | Troponin T levels and risks of 30-day outcomes in patients with the acute coronary syndrome:prospective verification in the GUSTO-Ⅳ trial显示文摘 | James S Armstrong P Califf R | | 0，，03： | 1 |
| 13 | Time-lapse in the IVF-lab:how should we assess potential benefit?显示文摘 | Armstrong S Vail A Mastenbroek S | 2015 | Hum Reprod2015，30，： | 1 |
| 14 | Dietary polyunsaturated fat uniquely suppress rat liver fatty acid synthase and s14m RNA content显示文摘 | Clarke S D Armstrong M K Jump D B | 1990 | Journal of Nutrition1990，120，： | 1 |
| 15 | Bioavailability of phosphorus inputs to lakes 显示文摘 | Sonzogni W C Chapra S C Armstrong D E | 1982 | Journal of Environmental Quality1982，11，4： | 1 |
| 16 | DNA barcodes for biosecurity: invasive species identification显示文摘 | Armstrong K F Ball S L | 2005 | Phil Trans R Soc B2005，360，： | 1 |
| 17 | Differentiation of Hunmn Embryonic Stem Cells into Corneal Epithelia|-like Cells by In Vitro Replication of theComeal Epithelial Stem Cell Niche 显示文摘 | Ahtnad S Stewart R Yung S Kolli S Armstrong L Stojkovie M Figueiredo F IAko M | 2007 | StemCells2007，255，： | 1 |
| 18 | Statistical algorithm for nonuniformity correction in focal plane arrays显示文摘 | Hayat M M Tortes S N Armstrong E | 1999 | Applied Optics1999，38，： | 1 |
| 19 | The Incentives for Tax Planning显示文摘 | Armstrong C S Blouin J L Larcker D F | | 0，，1： | 1 |
| 20 | Left ventricular function after mitral valve surgery显示文摘 | David T E Armstrong S Sun Z | | 0，，： | 1 |
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