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| 1 | Modulation of faecal metagenome in Crohn's disease:Role of microRNAs as biomarkers显示文摘BACKGROUND The gut microbiota plays a key role in the maintenance of intestinal homeostasis and the development and activation of the host immune system. It has been shown that commensal bacterial species can regulate the expression of host genes. 16 S rRNA gene sequencing has shown that the microbiota in inflammatory bowel disease(IBD) is abnormal and characterized by reduced diversity. Micro RNAs(miRNAs) have been explored as biomarkers and therapeutic targets, since they are able to regulate specific genes associated with Crohn's disease(CD). In this work, we aim to investigate the composition of gut microbiota of active treatment-na?ve adult CD patients, with miRNA profile from gut microbiota.AIM To investigate the composition of gut microbiota of active treatment-na?ve adult CD patients, with miRNA profile from gut microbiota.METHODS Patients attending the outpatient clinics at Valme University Hospital without relevant co-morbidities were matched according to age and gender. Faecal samples of newonset CD patients, free of treatment, and healthy controls were collected. Faecal samples were homogenized, and DNA was amplified by PCR using primers directed to the 16 S bacterial rRNA gene. Pyrosequencing was performed using GS-Junior platform. For sequence analysis, MGRAST server with the database Ribosomal Project was used. MiRNA profile and their relative abundance were analyzed by quantitative PCR.RESULTS Microbial community was characterized using 16 S rRNA gene sequencing in 29 samples(n = 13 CD patients, and n = 16 healthy controls). The mean Shannon diversity was higher in the healthy control population compared to CD group(5.5 vs 3.7). A reduction in Firmicutes and an increase in Bacteroidetes were found. Clostridia class was also significantly reduced in CD. Principal components analysis showed a grouping pattern, identified in most of the subjects in both groups, showing a marked difference between control and CD groups. A functional metabolic study showed that a lower metabolism of carbohydrates(P = 0.000) was found in CD group, while the metabolism of lipids was increased. In CD patients, three miRNAs were induced in affected mucosa: mir-144(6.2 ± 1.3 fold), mir-519(21.8 ± 3.1) and mir-211(2.3 ± 0.4). CONCLUSION Changes in microbial function in active non-treated CD subjects and three miRNAs in affected vs non-affected mucosa have been found. miRNAs profile may serve as a biomarker. | María Rojas-Feria Teresa Romero-García Jose Angel Fernández Caballero-Rico Helena Pastor Ramírez Marta Avilés-Recio Manuel Castro-Fernandez Natalia Chueca Porcuna Manuel Romero-Gomez Federico García Lourdes Grande JoséA Del Campo | 2018 | World Journal of Gastroenterology2018，24，46： | 7 |
| 2 | Coronary physiology assessment in the catheterization laboratory显示文摘Physicians cannot rely solely on the angiographic appearance of epicardial coronary artery stenosis when evaluating patients with myocardial ischemia. Instead, sound knowledge of coronary vascular physiology and of the methods currently available for its characterization can improve the diagnostic and prognostic accuracy of invasive assessment of the coronary circulation, and help improve clinical decision-making. In this article we summarize the current methods available for a thorough assessment of coronary physiology. | Felipe Díez-delhoyo Enrique Gutiérrez-Ibanes Gerard Loughlin Ricardo Sanz-Ruiz María Eugenia Vázquez-álvarez Fernando Sarnago-Cebada Rocío Angulo-Llanos Ana Casado-Plasencia Jaime Elízaga Francisco Fernández Avilés Diáz | 2015 | World Journal of Cardiology2015，7，9： | 2 |
| 3 | Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection显示文摘Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components.Dendritic cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease severity.In this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory markers.Remarkably,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 infection.Moreover,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were observed.These findings contribute to a better understanding of the immunological sequelae of COVID-19. | Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-Domínguez Juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-González Luis Fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez JoséManuel Lomas Claudio Bueno Rosario Amaya JoséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo Juan Delgado Antonio Domínguez-Petit Alejandro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-Delgado Manuel García-Gutiérrez María Luisa Gascón-Castillo Aurora González-Estrada Demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis Jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia Lanseros-Tenllado Carmina López Isabel López Álvaro López-Barrios Luis F.López-Cortés Rafael Luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez Luis Martín-Villén JoséMolina Aurora Morillo María Dolores Navarro-Amuedo Dolores Nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez Esther Pérez Manuel Poyato Julia Praena-Segovia Rafaela Ríos Cristina Roca-Oporto Jesús F.Rodríguez María Jesús Rodríguez-Hernández Santiago Rodríguez-Suárez Ángel Rodríguez-Villodres Nieves Romero-Rodríguez Ricardo Ruiz Zida Ruiz de Azua Celia Salamanca Sonia Sánchez Víctor Manuel Sánchez-Montagut César Sotomayor Alejandro Suárez Benjumea Javier Toral | 2021 | Cellular & Molecular Immunology2021，18，9： | 2 |
| 4 | Effective use of FibroTest to generate decision trees in hepatitis C显示文摘AIM:To assess the usefulness of FibroTest to forecast scores by constructing decision trees in patients with chronic hepatitis C.METHODS:We used the C4.5 classification algorithm to construct decision trees with data from 261 patients with chronic hepatitis C without a liver biopsy.The FibroTest attributes of age,gender,bilirubin,apolipoprotein,haptoglobin,α2 macroglobulin,and γ-glutamyl transpeptidase were used as predictors,and the FibroTest score as the target.For testing,a 10-fold cross validation was used.RESULTS:The overall classification error was 14.9%(accuracy 85.1%).FibroTest's cases with true scores of F0 and F4 were classified with very high accuracy(18/20 for F0,9/9 for F0-1 and 92/96 for F4) and the largest confusion centered on F3.The algorithm produced a set of compound rules out of the ten classification trees and was used to classify the 261 patients.The rules for the classification of patients in F0 and F4 were effective in more than 75% of the cases in which they were tested.CONCLUSION:The recognition of clinical subgroups should help to enhance our ability to assess differences in fibrosis scores in clinical studies and improve our understanding of fibrosis progression. | Dana Lau-Corona Luís Alberto Pineda Héctor Hugo Avilés Gabriela Gutiérrez-Reyes Blanca Eugenia Farfan-Labonne Rafael Núez-Nateras Alan Bonder Rosalinda Martínez-García Clara Corona-Lau Marco Antonio Olivera-Martínez Maria Concepción Gutiérrez-Ruiz Guillermo Robles-Díaz David Kershenobich | 2009 | World Journal of Gastroenterology2009，15，21： | 2 |
| 5 | Cranioplasty: cosmetic or therapeutic 显示文摘 | Dujovny M Aviles A Agner C el al | 1997 | Surg Neurol1997，47，： | 1 |
| 6 | Avian color perception predicts behavioral responses to experimental brood parasitism in chaffinches显示文摘 | Avilés JM Vikan JR Fossoy F Antonov A Moksnes A R(o)skaft E Stokke BG | | 0，，： | 1 |
| 7 | Nasal NK/T - cell lymphoma with disseminated disease treated with aggressive combined therapy显示文摘 | Aviles A Neri N Fernandez R | 2003 | Med Oncol2003，20，1： | 1 |
| 8 | Effect of a wound healing electromagnetic field on inflammatory cytokine gene expression in rats显示文摘 | Jasti A C Wetzel B J Aviles H | 2001 | Biomed Sci Instrum2001，37，： | 1 |
| 9 | Testicular lymphoma: organ---specific treatment did not improve outcome 显示文摘 | Aviles A Neri N Huerta--GuzmanJ | 2004 | Oncology2004，67，34： | 1 |
| 10 | Electrical and piezoresistive properties of multi-walled carbon nanotube/polymer composite films aligned by an electric field显示文摘 | Oliva-Avile's A I Avile's F Sosa V | 2011 | Carbon2011，49，9： | 1 |
| 11 | Nasal NK/T cell lymphoma with disseminated disease treated with aggressive combined therapy显示文摘 | Aviles A Neri N Fernandez R | 2003 | Med Oncol2003，20，1： | 1 |
| 12 | Rituximab and chemotherapy in primary gastric lymphoma显示文摘 | Aviles A Castafieda C Cleto S | 2009 | Cancer Biother Radiopharm2009，24，1： | 1 |
| 13 | Fractal analysis applied to characteristic segments of the san andreas fault显示文摘 | Aviles C A Scholz C H | 1987 | Journal of Geophysical Research1987，92，1： | 1 |
| 14 | Mesh migration following repaiar of inguinal hernia: a case report and review of literature 显示文摘 | Agrawal A Avill R | 2006 | Hernia2006，10，1： | 1 |
| 15 | Primary breastlymphoma:results of a controlled clinical trial显示文摘 | Avils A Delgado S Nambo MJ | 2005 | Oncology2005，69，3： | 1 |
| 16 | Treatment of non-Hodgkin' s lymphoma of Waldeyer' s ring: radiotherapy versus chemothera- py versus combined therapy 显示文摘 | Aviles A Delgado S Ruiz H | 1996 | Eur J Cancer B Oral Oncol1996，32，1： | 1 |
| 17 | Identification by sequencing based typing and complete coding region analysis of three new HLA class Ⅱ alleles:DRB3 * 0210,DRB3 * 0211and DQB1 * 0310显示文摘 | Santos S Aviles MJ | 2000 | Tissue Antigens2000，56，4： | 1 |
| 18 | The role of genotype in 104 cases of diffuse large B-cell lymphoma primary of breast显示文摘 | Aviles A Neri N Nambo M J | 2012 | Am J Clin Oncol2012，35，2： | 1 |
| 19 | The role of genotype in 104 cases of diffuse large B-cell lymphoma primary of breast 显示文摘 | Aviles A Neff N Nambo MJ | 2012 | Am J Clin Oneol2012，35，2： | 1 |
| 20 | Treatment of nonHodgkin's lymphoma of Waldeyer's ring:radiotherapy versus chemotherapy versus combined therapy显示文摘 | Aviles A Delgado S Ruiz H | 1996 | Eur J Cancer B Oral Oncol1996，32，1： | 1 |
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