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| 1 | Highly Pathogenic H5N1 Influenza Virus Infection in Migratory Birds显示文摘 | Jinhua Liu et al.College of Veterinary Medicine, China Agricultural University, Beijing 100094, China. | 2005 | 中国实验动物学报2005，13，S1： | 114 |
| 2 | Value of ecosystem services in China显示文摘The function and services are the important components of the life-support system in theplanet, as well as the basic elements for sustainable development of environment and society. It is a must to evaluate it for incorporating it with the social-economic system. It is also an important approach to draw the public attention on the environmental and ecosystem conservation. In this study, the ecosystem function and services in China were estimated by employing the classification and economic parameters from Costanza et al. The type and area of terrestrial ecosystems were extracted from Vegetation Map of China (1:4 000 000), and then the distribution map of ecosystem services of China was drawn. According to our calculation, the total value of ecosystem services in China is 77 834.48×108 RMB yuan per annum. The value for terrestrial ecosystem is 56 098.46×108 yuan per annum, and that for marine ecosystem is 21 736.02×108 yuan per annum. The value of ecosystem services in China is 1.73 times bigger | CHEN Zhongxin & ZHANG XinshiLaboratory of Quantitative Vegetation Ecology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China Current address: Institute of Natural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China | 2000 | Chinese Science Bulletin2000，45，10： | 128 |
| 3 | Construction of a genetic linkage map for cotton based on SRAP显示文摘A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers. | LIN Zhongxu, ZHANG Xianlong, NIE Yichun, HE Daohua & WU Maoqing National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China | 2003 | Chinese Science Bulletin2003，48，19： | 111 |
| 4 | Progress in the bionic study on anti-adhesion and resistance reduction of terrain machines显示文摘The theoretical studies of bionics of machinery have great scientific significance, and the development of bionic machines has large practical values in the field of engineering and technology. Through the rigorous selection process of evolution, the survived living organisms have successfully developed outstanding abilities to adapt to their surroundings and to reproduce their offspring. In this review,we interpreted the fundamental principles of anti-adhesion and anti-resistance of soil animals by reviewing the current status in this research field and summarizing the work of the research group at Jilin University of China in the past decades. The principles and technologies used in morphology bionics,electric-osmosis bionics,flexibility bionics,configuration bionics and coupling bionics were examined.Finally,the applications of the engineering bionics and their extensive prospects were introduced. | REN LuQuanKey Laboratory for Terrain-Machine Bionics Engineering of Ministry of Education, School for Biology and Agricultural Engineering, Jilin University, Changchun 130025, China | 2009 | Science China(Technological Sciences)2009，52，2： | 64 |
| 5 | The Assessment and Utilization of Straw Resources in China显示文摘The study was undertaken to evaluate the collectable and usable volume of existing straw resources, its suitability for different ways of utilization and fully reorganization of its development potential in China. Based on the results on the stubble heights of major crops in the Huang-Huai-Hai area (the area along the Yellow River, Huai River, and Hai River), the evaluation of the collectable and usable coefficients, and the collectable and usable volumes of various straw resources in China were worked out during 2005. The respective collectable and usable volumes of straw resources were worked out on the basis of suitability as fuel, feed, fertilizer, and base material for edible mushrooms. The total collectable and utilizable quantity of straw in China was 685 950 000 t, and the mean collection coefficient was 0.81 during 2005. The quantity of straw residue and wasted straw accounted for 19%. The collectable and utilizable quantities of grain, cash crop, and other crop straw were 492 310 000, 162 610 000, and 31 030 000 t which accounted for 71.77, 23.71, and 4.52%, respectively of the total collectable and utilizable quantity of straw. The quantity of straw which could be used as fuel was 635 000 000 t, which accounted for 92.63% of total quantity of the straw in the country during 2005. Among the total quantity of collectable and utilizable straw in China during 2005, the quantities of straw suitable and unsuitable for being processed as feedstuff were 587 640 000 and 98 310 000 t, which accounted for 85.67 and 14.33%, respectively. The quantity of straw residue returned to the field and collectable and utilizable straw for direct field restoration was 616 000 000 t, which accounted for about three-fourths of the total straw yield, while the quantity of straw for cultivation of edible mushrooms and industrial processing was about 587 000 000 t, which accounted for more than 85% of the total collectable and utilizable straw. The evaluation results indicate that the collectable and utilizable quantity of straw in China is abundant and suitable for many purposes. | WANG Ya-jing, BI Yu-yun and GAO Chun-yu Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China | 2010 | Agricultural Sciences in China2010，9，12： | 53 |
| 6 | Effects of N Forms and Rates on Vegetable Growth and Nitrate Accumulation显示文摘Experiments were carried out on a vegetable field with Peking cabbage (Brassica pekinensis (Lour.)Rupr.), cabbage (Brassica chinensis var. Oleifera Makino and nemoto), green cabbage (Brassica chinensis L.), spinach (Spinacia oleracea L.) and rape (Brassica campestris L.) to study the effects of N forms and N rates on their growth and nitrate accumulation. The results indicated that application of ammonium chloride,ammonium nitrate, sodium nitrate and urea significantly increased the yields and nitrate concentrations of Peking cabbage and spinach. Although no significant difference was found in the yields after application of the 4 N forms, nitrate N increased nitrate accumulation in vegetables much more than ammonium N. The vegetable yields were not increased continuously with N rate increase, and oversupply of N reduced the plant growth, leading to a yield decline. This trend was also true for nitrate concentrations in some vegetables and at some sampling times. However, as a whole, the nitrate concentrations in vegetables were positively correlated with N rates. Thus, addition of N fertilizer to soil was the major cause for increases in nitrate concentrations in vegetables. Nitrate concentrations were much higher in roots, stems and petioles than in blades at any N rate. | WANG Zhao-Hui and LI Sheng-XiuCollege of Resources and Environmental Sciences, Northwestern Science and Technology University of Agriculture and Forestry, Yangling 712100 (China). E-mail: w-zhaohui@263.net | 2003 | Pedosphere2003，13，4： | 54 |
| 7 | Invasive mechanism and control strategy of Ageratina adenophora (Sprengel)显示文摘In order to ascertain the invasive mechanism and control strategy of the invasive Crofton weed, Ageratina adenophora, its ecological adaptability and population differentiation,the formation of single dominant population, displacement of native plants and sustainable management strategies were investigated. The present results helped to clarify and explain such issues as the adaptability post invasion,interaction and competition between inter-and intra-species and community resistance, thereby providing important references to researches on other invasive alien species. | WAN FangHao1, LIU WanXue1, GUO JianYing1, QIANG Sheng2, LI BaoPing2, WANG JinJun3, YANG GuoQing1, NIU HongBang1, GUI FuRong1, HUANG WenKun1, JIANG ZhiLin1 & WANG WenQi3 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China 3Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China | 2010 | Science China(Life Sciences)2010，53，11： | 42 |
| 8 | Variation of grain Cd and Zn concentrations of 110 hybrid rice cultivars grown in a low-Cd paddy soil显示文摘Enhanced Cd uptake and Zn depletion in rice grains and high potential for food Cd exposure by the high-yielding hybrid cultivars of China had been addressed.A field experiment was conducted in 2006 to determine the difference in grain Cd and Zn between cultivars.Total 110 cultivars including super rice and common hybrid rice cultivars were grown on a single paddy soil(Entic Haplaquept) with a neutral reaction and low total Cd content.Grain Cd and Zn concentrations were determined with graphite atomic adsorption spectrophotometer(GFAAS) and flame atomic adsorption spectrophotometer(AAS) respectively.Wide variation of Cd content in grain was found in a range of 0.004-0.057 mg/kg,while the Zn content in a range of 10.25-30.06 mg/kg among the cultivars.Higher Cd but lower Zn concentration in grains of super rice cultivars was observed compared to the common hybrid ones.A highly significant positive linear correlation of grain Cd/Zn with grain Cd was found for super rice and common hybrid cultivars,meanwhile much higher slope for these hybrid cultivars than the reported non-hybrid cultivars was also observed.Using the limit value of the Chinese chemical guidelines for foods(MOHC and SSC,2005),calculated potential risk of food Cd exposure with 'Zn hungry' through diet intake was prominent with all the studied 110 hybrid rice cultivars,possessing high potential health problems for rice production in South China using the super rice cultivars.Breeding of genotypes of rice cultivars with low grain Cd and low Cd/Zn ratio is needed for rice production in acidic red soils where Cd bioavailability is prevalently high. | SHI Jing,LI Lianqing,PAN Genxing Institute of Resource,Ecosystem and Environment of Agriculture,Nanjing Agricultural University,Nanjing 210095,China. | 2009 | Journal of Environmental Sciences2009，21，2： | 44 |
| 9 | Effect of Silicon on Leaf Ultrastructure, Chlorophyll Content and Photosynthetic Activity of Barley Under Salt Stress显示文摘Two contrasting cultivars of barley (Horderm vykgare L.): Kepin No. 7 (salt sensitive), and Jian 4 (salt tolerant) were grown in a hydroponics system with 2 NaCl levels: 60 mmol NaCl L-1 and 120 mmol NaCl L-1, and 3 Si levels: 0 mmol Si L-1, 0.5 mmol Si L-1 and 1.0 mmol Si L-1 (as silicic acid). Compared with the plants treated with 60 mmol NaCl L-1 alone, the leaf chlorophyll contents of plants treated with salt and Si increased significantly for salt-sensitive cultivar at tillering stage, but for salt-tolerant cultivar,the addition of Si resulted in an obvious increase in the leaf chlorophyll content of plants exposed to 120 mmol NaCl L-1. However, this Si-enhancement of leaf chlorophyll content was also observed in the salttolerant plants at jointing stage, but not in the salt-sensitive plants. Moreover, leaf chlorophyll content was consistently higher for the salt-tolerant cultivar than for the salt-sensitive cultivar irrespective of salt and/or Si treatment. Compared with the plants treated with salt alone, net CO2 assimilation rate in plant leaves increased significantly for both cultivars when trested with salt and Si. The addition of Si to the salt treatment was found to improve the cell ultrastructure of leaves. Under salt stress condition, the double membranes of chloroplasts disappeared, but membrane integrity was markedly improved in the salt treatment supplemented with Si. Silicon was also found to ameliorate the damage to the ultrastructure of chloroplast granae which appeared to be disintegrated and vague in salt treatments without added Si. The results support previous work which showed that Si decreases the permeability of plasma membranes of salt-stressed barley, thus mitigating salt damage. | LIANG YONGCHAO (Department of Plant Nutrition, College of Natural Reaources and Environmental Science, and Ministry of Agriculture Key Laboratory of Crop Growth and Regulation, Nanjing Agricultural University, Nanjing 210095 China) | 1998 | Pedosphere1998，8，4： | 43 |
| 10 | H5N1 avian influenza in China显示文摘H5N1 highly pathogenic avian influenza virus was first detected in a goose in Guangdong Province of China in 1996. Multiple genotypes of H5N1 viruses have been identified from apparently healthy waterfowl since 1999. In the years 2004-2008, over 100 outbreaks in domestic poultry occurred in 23 provinces and caused severe economic damage to the poultry industry in China. Beginning from 2004, a culling plus vaccination strategy has been implemented for the control of epidemics. Since then, over 35420000 poultry have been depopulated, and over 55 billion doses of the different vaccines have been used to control the outbreaks. Although it is logistically impossible to vaccinate every single bird in China due to the large poultry population and the complicated rearing styles, there is no doubt that the increased vaccination coverage has resulted in decreased disease epidemic and environmental virus loading. The experience in China suggests that vaccination has played an important role in the protection of poultry from H5N1 virus infection, the reduction of virus load in the environment, and the prevention of H5N1 virus transmission from poultry to humans. | CHEN HuaLan Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China | 2009 | Science China(Life Sciences)2009，52，5： | 36 |
| 11 | Ideotype root architecture for efficient nitrogen acquisition by maize in intensive cropping systems显示文摘The use of nitrogen(N) fertilizers has contributed to the production of a food supply sufficient for both animals and humans despite some negative environmental impact.Sustaining food production by increasing N use efficiency in intensive cropping systems has become a major concern for scientists,environmental groups,and agricultural policymakers worldwide.In high-yielding maize systems the major method of N loss is nitrate leaching.In this review paper,the characteristic of nitrate movement in the soil,N uptake by maize as well as the regulation of root growth by soil N availability are discussed.We suggest that an ideotype root architecture for efficient N acquisition in maize should include(i) deeper roots with high activity that are able to uptake nitrate before it moves downward into deep soil;(ii) vigorous lateral root growth under high N input conditions so as to increase spatial N availability in the soil;and(iii) strong response of lateral root growth to localized nitrogen supply so as to utilize unevenly distributed nitrate especially under limited N conditions. | MI GuoHua,CHEN FanJun,WU QiuPing,LAI NingWei,YUAN LiXing & ZHANG FuSuo* Key Laboratory of Plant Nutrition,MOA Key Laboratory of Plant-Soil Interaction,MOE College of Resources and Environmental Science,China Agricultural University,Beijing 100193,China | 2010 | Science China(Life Sciences)2010，53，12： | 35 |
| 12 | Formation and Water Stability of Aggregates in Red Soils as Affected by Organic Matter显示文摘FormationandWaterStabilityofAggregatesinRedSoilsasAffectedbyOrganicMatter￥ZHANGMINGKUI;HEZHENLI;CHENGUOCHAO;HUANGCHANGYONGIan... | ZHANG MINGKUI HE ZHENLI CHEN GUOCHAO HUANG CHANGYONGI andM. J. WILSON￣2(￣1DePartment of Land Use and Applied Chemistry, Zhejiang Agricultural University, Hangzhou 31O029(China))(￣2Soils and Soil Microbiology Division, Macaulay Land Use Research Inst | 1996 | Pedosphere1996，6，1： | 38 |
| 13 | Changes in plant biomass and species composition of alpine Kobresia meadows along altitudinal gradient on the Qinghai-Tibetan Plateau显示文摘Alpine Kobresia meadows are major vegetation types on the Qinghai-Tibetan Plateau. There is growing concern over their relationships among biodiversity, productivity and environments. Despite the im-portance of species composition, species richness, the type of different growth forms, and plant bio-mass structure for Kobresia meadow ecosystems, few studies have been focused on the relationship between biomass and environmental gradient in the Kobresia meadow plant communities, particularly in relation to soil moisture and edaphic gradients. We measured the plant species composition, her-baceous litter, aboveground and belowground biomass in three Kobresia meadow plant communities in Haibei Alpine Meadow Ecosystem Research Station from 2001 to 2004. Community differences in plant species composition were reflected in biomass distribution. The total biomass showed a de-crease from 13196.96±719.69 g/m2 in the sedge-dominated K. tibetica swamp to 2869.58±147.52 g/m2 in the forb and sedge dominated K. pygmaea meadow, and to 2153.08±141.95 g/m2 in the forbs and grasses dominated K. humilis along with the increase of altitude. The vertical distribution of below-ground biomass is distinct in the three meadow communities, and the belowground biomass at the depth of 0-10 cm in K. tibetica swamp meadow was significantly higher than that in K. humilis and K. pygmaea meadows (P<0.01). The herbaceous litter in K. tibetica swamp was significantly higher than those in K. pygnaeca and K. humilis meadows. The effects of plant litter are enhanced when ground water and soil moisture levels are raised. The relative importance of litter and vegetation may vary with soil water availability. In the K. tibetica swamp, total biomass was negatively correlated to species richness (P<0.05); aboveground biomass was positively correlated to soil organic matter, soil moisture, and plant cover (P<0.05); belowground biomass was positively correlated with soil moisture (P<0.05). However, in the K. pygnaeca and K. humilis meadow communities, aboveground biomass was posi-tively correlated to soil organic matter and soil total nitrogen (P<0.05). This suggests that the distribu-tion of biomass coincided with soil moisture and edaphic gradient in alpine meadows. | WANG ChangTing1,3, CAO GuangMin1, WANG QiLan1, JING ZengChun1, DING LuMing1 & LONG RuiJun2 1 Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China 2 College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730070, China 3 Graduate University of the Chinese Academy of Sciences, Beijing 100039, China | 2008 | Science China(Life Sciences)2008，51，1： | 31 |
| 14 | Invasive mechanism and management strategy of Bemisia tabaci(Gennadius) biotype B:Progress report of 973 Program on invasive alien species in China显示文摘Bemisia tabaci(Gennadius) biotype B,called a 'superbug',is one of the most harmful biotypes of this species complex worldwide.In this report,the invasive mechanism and management of B.tabaci biotype B,based on our 5-year studies,are presented.Six B.tabaci biotypes,B,Q,ZHJ1,ZHJ2,ZHJ3 and FJ1,have been identified in China.Biotype B dominates the other biotypes in many regions of the country.Genetic diversity in biotype B might be induced by host plant,geographical conditions,and/or insecticidal application.The activities of CarE(carboxylesterase) and GSTs(glutathione-S-transferase) in biotype B reared on cucumber and squash were greater than on other host plants,which might have increased its resistance to insecticides.The higher activities of detoxification enzymes in biotype B might be induced by the secondary metabolites in host plants.Higher adaptive ability of biotype B adults to adverse conditions might be linked to the expression of heat shock protein genes.The indigenous B.tabaci biotypes were displaced by the biotype B within 225 d.The asymmetric mating interactions and mutualism between biotype B and begomoviruses via its host plants speed up widespread invasion and displacement of other biotypes.B.tabaci biotype B displaced Trialeurodes vaporariorum(Westwood) after 4-7 generations under glasshouse conditions.Greater adaptive ability of the biotype B to adverse conditions and its rapid population increase might be the reasons of its successful displacement of T.vaporariorum.Greater ability of the biotype B to switch to different host plants may enrich its host plants,which might enable it to better compete with T.vaporariorum.Native predatory natural enemies possess greater ability to suppress B.tabaci under field conditions.The kairomones in the 3rd and 4th instars of biotype B may provide an important stimulus in host searching and location by its parasitoids.The present results provide useful information in explaining the mechanisms of genetic diversity,evolution and molecular eco-adaptation of biotype B.Furthermore,it provides a base for sustainable management of B.tabaci using biological and ecological measures. | WAN FangHao1,ZHANG GuiFen1,LIU ShuSheng2,LUO Chen3,CHU Dong4,ZHANG YouJun4,ZANG LianSheng2,JIU Min2,Lü ZhiChuang1,CUI XuHong1,ZHANG LiPing4,ZHANG Fan3,ZHANG QingWen5,LIU WanXue1,LIANG Pei5,LEI ZhongRen1 & ZHANG YongJun1 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Acadeny of Agriculture Sciences,Beijing 100094,China 2 Institute of Applied Entomology,Zhejiang University,Hangzhou 310029,China 3 Institute of Plant and Environment Protection,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100089,China 4 Institute of Vegetables and Flowers,Chinese Acadeny of Agricullare Sciences,Beijing 100081,China 5 Department of Entomology,China Agriculture University,Beijing 100094,China | 2009 | Science China(Life Sciences)2009，52，1： | 30 |
| 15 | The characteristics of CO_2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice显示文摘With PEPC, PPDK, NADP-ME and PEPC+ PPDK transgenic and untransformed rice (Orysa sativa L.), the activities of related C4 photosynthesis enzymes, the chlorophyll fluorescence parameters, CO2 exchange and other physiological indexes were compared, in which the physiological characteristics of PEPC transgenic rice were mainly studied. The results were as follows: (i) The activities of PEPC in PEPC transgenic rice were 20-fold higher than those in untransformed rice; the light-saturation photosynthetic rates and the carboxylation efficiency of PEPC transgenic rice were increased by 55% and 50% more than those of untransformed rice, respectively, while the CO2 compensation point decreased by 27%. (ii) The PS II photochemical efficiency (Fv/Fm) and photochemical quenching (qp) of transgenic PEPC rice decreased less in comparison with those of untransformed rice after the treatment with high light intensity (3 h) or methyl viologen (MV), a photooxidative reagent, which demonstrated that the tolerance of PEPC | JIAO Demao, LI Xia, HUANG Xueqing, CHI Wei, KUANG Tingyun & Ku SB Maurice1. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nangjing 210014, China 2. Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China 3. Botany Department, Washington State University, Pullman, WA 99164-4236, USA | 2001 | Chinese Science Bulletin2001，46，13： | 29 |
| 16 | Genotypic Differences in Grain Yield and Physiological Nitrogen Use Efficiency Among Rice Cultivars显示文摘Efficient use of N in agricultural practice can increase yield, decrease production costs and reduce the risk of environmental pollution. Effects of N fertilizer application rates on grain yield and physiological N use efficiency (PE) in relation to the accumulation and redistribution of biomass and N in rice (Oryza sativa L.) cultivars were studied at two experimental farms of Nanjing Agricultural University, Nanjing, China in 2004. Three high N use efficiency (NUE) rice cultivars (Wuyunjing 7, Nanguang and 4007) and one low NUE rice cultivar (Elio) with similar growth patterns were studied under seven N rates (0, 60, 120, 180, 240, 300 and 360 kg ha -1 ). Grain yield increased with the N application rate and attained plateau at 180 kg N ha -1 for rice cultivars at each site. Increasing N rate decreased PE for biomass and grain yield. Grain yield and PE of Elio were about 20% and 18% lower than those of high NUE cultivars. Differences in biomass, N accumulation and N redistribution were observed at the post-heading stage among rice cultivars with differing NUEs. The less reproductive tillers of Elio resulted in less demand for C and N during grain filling, thus leading to lower PE of Elio compared with the high NUE rice cultivars. | ZHANG Ya-Li, FAN Jian-Bo, WANG Dong-Sheng and SHEN Qi-Rong2College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095 (China) | 2009 | Pedosphere2009，19，6： | 30 |
| 17 | Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages显示文摘An advanced backcross population of rice was used to identify the quantitative trait locus (QTL) controlling the cold-tolerance at booting to flowering stages. The recipient, Guichao 2 (GC2), was a commercial Indica rice; the donor Dongxiang common wild rice, was an accession of common wild rice (DXCWR, Oryza rufipogon Griff.). Three QTLs for cold-tolerance were detected on chromosomes 1, 6 and 11. Two of them coming from DXCWR could enhance the cold-tolerance of the backcross progenies. Moreover, one sterility QTL that could reduce the seed set rate of the backcross progenies by 78% was mapped on chromosome 5. | LIU Fengxia, SUN Chuanqing, TAN Lubin, FU Yongcai, LI Dejun & WANG Xiangkun Department of Plant Genetics and Breeding, China Agricultural University, National Key Laboratory of Agrobiology, Key Laboratory of Crop Genetic Improvement and Genome of the Ministry of Agriculture, Beijing 100094, China | 2003 | Chinese Science Bulletin2003，48，19： | 29 |
| 18 | Analysis of DNA methylation in different maize tissues显示文摘DNA methylation plays an important role in gene expression regulation during biological development and tissue differentiation in plants. This study adopted methylation-sensitive Amplified fragment length polymorphism (AFLP) to compare the levels of DNA cyto- sine methylation at CCGG sites in tassel, bracteal leaf, and ear leaf from maize inbred lines, 18 White and 18 Red, respectively, and also examined specific methylation patterns of the three tissues. Significant differences in cytosine methylation level among the three tissues and the same changing tendency in two inbred lines were detected. Both MSAP (methylation sensitive amplification polymorphism) ratio and full methylation level were the highest in bracteal leaf, and the lowest in tassel. Meanwhile, different methylation levels were ob- served in the same tissue from the inbred lines, 18 White and 18 Red. Full methylation of internal cytosine was the dominant type in the maize genome. The differential methylation patterns in the three tissues were observed. In addition, sequencing of nine differentially me- thylated fragments and the subsequent blast search revealed that the cytosine methylated 5′-CCGG-3′sequences were distributed in re- peating sequences, in the coding and noncoding regions. Southern hybridization was used to verify the methylation polymorphism. These results clearly demonstrated the power of the MSAP technique for large-scale DNA methylation detection in the maize genome, and the complexity of DNA methylation change during plant growth and development. The different methylation levels may be related to specific gene expression in various tissues. | Yanli Lu, Tingzhao Rong, Moju Cao Maize Research Institute of Sichuan Agriculture University/Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education, Ya’an, 625014, China | 2008 | Journal of Genetics and Genomics2008，35，1： | 27 |
| 19 | Use of Controlled Release Fertilizer for Increasing N Efficiency of Direct Seeding Rice显示文摘Field trials on a silt-loamy paddy soil derived from shallow-sea deposit in direct seeding rice fields were conducted in Zhejiang, China, in 1996 to compare N efficiency of controlled release fertilizers (LP fertilizers) with the conventional urea. Six treatments including CK (no N fertilizer), conventional urea and different types of LP fertilizers at different rates were designed for two succeeding crops of early and late rice. A blend of different types of LP fertilizers as a single preplant 'co-situs' application released N in a rate and amount synchronizing with uptake pattern of direct seeding rice. A single preplant application of the LP fertilizers could meet the N requirement of rice for the whole growth period without need of topdressing. Using LP fertilizer blends, equivalent grain yields could be maintained even if the N fertilization rates were reduced by 25%~50% compared with the conventional urea. Agronomic efficiency of the LP fertilizers was 13.6%~ 86.4% higher than that of the conventional urea in early rice and 100%~164.1% in late rice, depending on the amounts of the LP fertilizers applied. N fertilizer recovery rate increased from 27.4% for the conventional application of urea to 41.7%~54.l% for the single preplant 'co-situs' application of the LP fertilizers. Use of the LP fertilizers was promising if the increase in production costs due to the high LP fertilizer prices could be compensated by increase in yield and N efficiency, reduction in labor costs and improvement in environment. | FU JIANRONG, ZHU YUANHONG and JIANG LINA Institute of Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021 (China) | 2001 | Pedosphere2001，11，4： | 27 |
| 20 | Ability of Agrogyron elongatum to accumulate the single metal of cadmium, copper, nickel and lead and root exudation of organic acids显示文摘Agrogyron elongatum were grown in nutrient solution containing moderate to high amounts of separate heavy metal of Cd, Cu, Ni and Pb in a greenhouse for a 9-day. Cd, Cu, Ni and Pb generally led to decrease in the elongation of roots although the length of seedlings exposed to Cd and Pb at 0.05 and 0 5 mg/L showed to be slightly greater than that of controls. Of the four metals in the experiment, Pb was absorbed and accumulated to the highest level, with the concentrations of 92754 mg/kg dry weight (DW) in roots and 11683 mg/kg DW in shoots. Cd was moderately accumulated in Agrogyron elongatum, but the maximum bioaccumulation coefficients (BCs) for roots and shoots were observed. The patterns for Cu and Ni uptake and distribution in plants differed from those of Pb and Cd, as it was showed that the shoot accumulation of Cu and Ni was significantly higher than in roots. A. elongatum had the highest Ni concentration in shoots (30261 mg/kg DW) at the external concentration of 250 mg/L. Cu ranked second, with a shoot concentration of 12230 mg/kg DW when 50 mg/L Cu in solution was applied. For the four trace elements tested, the highest concentrations in shoots decreased by the order of Ni>Cu>Pb>Cd (mg/kg DW), and those in roots were Pb>Cd>Ni>Cu (mg/kg DW). Malic, oxalic and citric acids exuded by roots exposed to 1 and 50 mg/L of the metals were detected. Release of organic acids from plants significantly differed among the metal treatments. Cu was most effectively in inducing root exudation of the three types of organic acids. Cd, and Ni were also the inducers of secretion of malic and oxalic acids. With reference of Pb, a small amounts of malic and oxalic acids were detected in the root exudates, but few quantities of citric acid were found. However, no correlation between alternations in root exudation of organic acids and metal accumulation could be established. | YANG Hong 1, Jongathan W.C.Wong 2, YANG Zhi min\+1, ZHOU Li xiang 1(1. College of Science, Nanjing Agricultural University, Nanjing\ 210095, China. 2. Department of Biology, Hong Kong Baptist University, China) | 2001 | Journal of Environmental Sciences2001，13，3： | 26 |
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