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| 1 | Study of the antioxidant capacity of Miscanthus sinensis lignins显示文摘 | Araceli García Ana Toledano Maria ángeles Andrés Jalel Labidi | 2010 | Process Biochemistry2010，，6： | 1 |
| 2 | Vinyl sulfonyl chemistry-driven unidirectional transport of a macrocycle through a[2]rotaxane显示文摘By applying a combination of the coupling-and-decoupling(CAD)chemistry of the vinyl sulfonate group with the click thia-Michael addition to the vinyl sulfone group(MAVS)we performed the irreversible unidirectional transportation of the ring through the linear component in a[2]rotaxane by a chemically and pHdriven flashing energy ratchet mechanism. | Arthur H.G.David Pablo García-Cerezo Araceli G.Campaña Francisco Santoyo-González Victor Blanco | 2022 | Organic Chemistry Frontiers2022，9，3： | 0 |
| 3 | Prevalence of hepatocarcinoma-related hepatitis B virus mutants in patients in grey zone of treatment显示文摘BACKGROUND Antiviral treatment of patients with chronic hepatitis B(CHB)in the grey zone of treatment comands risk management in order to optimize the health outcome.In this sense,the identification of HBV mutants related with an increased risk of hepatocellular carcinoma(HCC)could be useful to identify subpopulations with potential indication of antiviral treatment.AIM To analyze the prevalence/persistence of hepatitis B virus(HBV)preS and basal core promoter(BCP)/precore/core variants associated to HCC development in CHB patients in the grey zone.METHODS Work was designed as a longitudinal retrospective study,including 106 plasma samples from 31 patients with CHB in the grey zone of treatment:Hepatitis B e antigen negative,HBV-DNA levels between 12-20000 IU/mL,normal or discordant transaminase levels during follow up and mild/moderate necroinflammatory activity in liver biopsy or Fibroscan(up to 9.5 kPa).Serum HBVDNA was tested using the Abbott Real Time HBV Assay and the BCP/precore/core and the hepatitis B surface antigen(HBsAg)coding regions were analyzed in positive samples by PCR/bulk-sequencing to identify the HCCrelated HBV mutants.RESULTS High-risk HCC related mutants were detected in 24(77%)patients:19(61%)in the BCP/precore/core,and 7(23%)in the HBsAg coding region(2 preS1 and 5 preS2 deletions).The prevalence of preS deletions was genotype-dependent:3/5(60%)patients with preS2 deletions and 1/2 with preS1 deletions were infected with the HBV-E genotype.Since HBV-E was the most prevalent in sub-Saharan patients,a correlation between preS deletions and ethnicity was also found:6/8(75%)sub-Saharan vs 1/19(5%)Caucasian patients had preS deletions(P=0.00016).Remarkably,this correlation was maintained in those patients infected with HBV-A,a minor genotype in sub-Saharan patients:2/2 patients infected with HBV-A from West Africa vs 0/6 of Caucasian origin had preS deletions.The HCC related variants were the major strains and persisted over time(up to 48 mo).Patients with preS deletions had a significant higher prevalence of F2 fibrosis stage than the negatives(57%vs 10%,P=0.0078).CONCLUSION HBV genetic analysis of selected populations,like sub-Saharans infected with HBV-E/A genotypes,will allow identification of subpopulations with risk of HCC development due to accumulation of high-risk HBV variants,thus commanding their increased clinical surveillance. | Ana Isabel Gil-García Antonio Madejón Irene Francisco-Recuero Ana López-López Emiliana Villafranca Miriam Romero Araceli García Antonio Olveira Rocío Mena Juan Ramón Larrubia Javier García-Samaniego | 2019 | World Journal of Gastroenterology2019，25，38： | 1 |
| 4 | Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration显示文摘AIM: To evaluate the effect of an extract of Geranium schiedeanum(Gs) as a hepatoprotective agent against ethanol(Et OH)-induced toxicity in rats. METHODS: Male Wistar rats weighing 200-230 g were subjected to a 70% partial hepatectomy(PH); they were then divided into three groups(groups 1-3). During the experiment, animals in group 1 drank only water. The other two groups(2-3) drank an aqueous solution of Et OH(40%, v/v). Additionally, rats in group 3 received a Gs extract daily at a dose of 300 mg/kg body weight intragastically. Subsequently, to identify markers of liver damage in serum, alanine aminotransferase, aspartate aminotransferase, albumin and bilirubin were measured by colorimetric methods. Glucose, triglyceride and cholesterol concentrations were also determined. In addition, oxidative damage was estimated by measuring lipid peroxidation [using thiobarbituric-acid reactive substances(TBARS)] in both plasma and the liver and by measuring the total concentration of antioxidants in serum and the total antioxidant capacity in the liver. In addition, a liver mass gain assessment, total DNA analysis and a morpho-histological analysis of the liver from animals in all three groups were performed and compared. Finally, the number of deaths observed in the three groups was analyzed.RESULTS: Administration of the Geranium shiedeanum extract significantly reduced the unfavorable effect of ethanol on liver regeneration(restitution liver mass: PHEt OH group 60.68% vs PH-Gs-Et OH group 69.22%). This finding was congruent with the reduced levels of hepatic enzymes and the sustained or increased levels of albumin and decreased bilirubin in serum. The extract also modified the metabolic processes that regulate glucose and lipid levels, as observed from the serum measurements. Lower antioxidant levels and the liver damage induced by Et OH administration appeared to be mitigated by the extract, as observed from the TBARs(PH-Et OH group 200.14 mmol/mg vs PH-Gs-Et OH group 54.20 mmol/mg; P < 0.05), total status of antioxidants(PH-Et OH group 1.43 mmol/L vs PH-Gs-Et OH group 1.99 mmol/L; P < 0.05), total antioxidant capacity values, liver mass gain and total DNA determination(PH-Et OH group 4.80 mg/g vs PH-Gs-Et OH 9.10 mg/g; P < 0.05). Overall, these processes could be related to decreased mortality in these treated animals.CONCLUSION: The administered extract showed a hepatoprotective effect, limiting the Et OH-induced hepatotoxic effects. This effect can be related tomodulating oxido-reduction processes. | Eduardo Madrigal-Santillán Mirandeli Bautista Juan A Gayosso-De-Lucio Yadira Reyes-Rosales Araceli Posadas-Mondragón ángel Morales-González Marvin A Soriano-Ursúa Jazmín García-Machorro Eduardo Madrigal-Bujaidar Iselaálvarez-González JoséA Morales-González | 2015 | World Journal of Gastroenterology2015，21，25： | 1 |
| 5 | Improvement of transfection with reprogramming factors in urine-derived cells显示文摘Human-induced pluripotent stem cells(iPSCs)are an accessible source of adult-derived,patient-specific pluripotent stem cells for use in basic research,drug discovery,disease modeling,and stem cell therapy.Improving the accessibility of methods to obtain iPSCs regardless of the cell source can enhance their clinical application.Therefore,our purpose is to report a simple protocol to obtain iPS-like cells from urine-derived renal epithelial cells(RECs)using different extracellular matrices and transfection reagents.In this study,we began by culturing urine-derived cells from healthy donors to establish a primary culture of renal epithelial cells,followed by their characterization.Subsequently,we generated iPS-like cells by transfecting renal epithelial cells(RECs)with vectors expressing Oct4,Sox2,L-Myc,Lin-28,and Klf4,and we compared the efficacy of different extracellular matrices and transfection reagents.The resultant iPS-like cells showed a human embryonic stem cell-like morphology and expressed the specific pluripotency markers Oct3/4,Nanog,Lin28,and Klf4.We concluded that Lipofectamine Stem Cell transfection reagent is more effective than FuGENE in obtaining iPS-like cells under the conditions tested.Moreover,the three matrices are similar in their efficiency of obtaining iPS-like cells.This report provides an experimental protocol for obtaining and generating iPS-like cells from urine samples for further cell therapy research on different human diseases. | OLIVIA A.ROBLES-RODRÍGUEZ MARÍA J.LOERA-ARIAS JOSÉJ.PÉREZ-TRUJILLO ARNULFO VILLANUEVA-OLIVO ERNESTO PICÓN-GALINDO LAURA VILLARREAL-MARTÍNEZ ADOLFO SOTO-DOMÍNGUEZ HUMBERTO RODRÍGUEZROCHA ARACELY GARCÍA-GARCÍA ODILA SAUCEDO-CÁRDENAS ROBERTO MONTES DE OCA-LUNA | 2020 | BIOCELL2020，44，3： | 0 |
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