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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | The role of the N-methyl-D-aspartate receptor in the proliferation of adult hippocampal neural stem and precursor cells显示文摘New neurons are continuously generated from resident pools of neural stem and precursor cells(NSPCs)in the adult brain.There are multiple pathways through which adult neurogenesis is regulated,and here we review the role of the N-methyl-D-aspartate receptor(NMDAR)in regulating the proliferation of NSPCs in the adult hippocampus.Hippocampal-dependent learning tasks,enriched environments,running,and activity-dependent synaptic plasticity,all potently up-regulate hippocampal NSPC proliferation.We first consider the requirement of the NMDAR in activity-dependent synaptic plasticity,and the role the induction of synaptic plasticity has in regulating NSPCs and newborn neurons.We address how specific NMDAR agonists and antagonists modulate proliferation,both in vivo and in vitro,and then review the evidence supporting the hypothesis that NMDARs are present on NSPCs.We believe it is important to understand the mechanisms underlying the activation of adult neurogenesis,given the potential that endogenous stem cell populations have for repopulating the hippocampus with functional new neurons.In conditions such as age-related memory decline,neurodegeneration and psychiatric disease,mature neurons are lost or become defective;as such,stimulating adult neurogenesis may provide a therapeutic strategy to overcome these conditions. | TAYLOR Chanel J HE RongQiao BARTLETT Perry F | 2014 | Science China(Life Sciences)2014，57，4： | 6 |
| 3 | Spinning magnets and Jehle's model of the electron显示文摘 | Bartlett D F Monroy J Reeves J | 1977 | Phys Rev D1977，16，： | 1 |
| 4 | Cloning and Growth of Multioptential Neural Precursors-Requirements for Proliferation and Differentiation显示文摘 | Kilpatrick T J Bartlett P F | 1993 | Neuron1993，10，2： | 1 |
| 5 | Growth and body composition of dairy calves fed milk replacers containing different amounts of protein at two feeding rates显示文摘 | Bartlett K S Mckeith F K Vandehaar M J | 2006 | J Anim Sci2006，84，： | 1 |
| 6 | Growth and body composition of dairy calves fed milk replacers containing different amounts of protein at two feeding rates 显示文摘 | Bartlett K S McKeith F K VandeHaar M J | 2006 | Journal of Animal Science2006，，84： | 1 |
| 7 | Imported fire ant toxic bait studies:eyaluatiOil of toxicants显示文摘 | Stringer C E Lofgren C S Bartlett F J | 1964 | J Econ Entomo1964，57，： | 1 |
| 8 | Communityacquired pneumonia in adults: guidelines for management,The infection Diseases society of America 显示文摘 | Bartlett J G Breiman R F Mandell L A | 1998 | Clin Infect Dis1998，26，4： | 1 |
| 9 | 显示文摘 | Bartlett R J Stanton J F | 1994 | Rev Comp Chem1994，，5： | 1 |
| 10 | Review of resistance fac- tors for steel:data collection显示文摘 | Schmidt B J Bartlett F M | 2010 | Canadian Journal of Civl Engineering2010，29，1： | 1 |
| 11 | Growth and body composition of dairy calves fed milk rephcers containing different amounts of prorein at two feeging rates显示文摘 | Bartlett K S McKeith F K VandeHaar M J | 2006 | J Animal Sci2006，84，： | 1 |
| 12 | Growth and body composition of dairy calves fed milk replacers containing different amounts of protein at two feeding rates 显示文摘 | BARTLETT K S MCKEITH F K VANDEHAAR M J | 2006 | J Anita Sei2006，84，： | 1 |
| 13 | Recent advances in topical ophthalmic drug delivery with lipid- based nanocarriers 显示文摘 | Gan L Wang J Jiang M Bartlett H Ouyang D Eperjesi F | 2013 | Drug Discov Today2013，18，56： | 1 |
| 14 | Update of practice guidelines for the management of community - acquired pneumonia in immunocompetent adults 显示文摘 | MANDELL L A BARTLETT J G DOWELL S F | 2003 | Clin Infect Dis2003，37，： | 1 |
| 15 | Growth and body composition of dairy calves fed milk replacers containing different amounts of protein at two feeding rates显示文摘 | BARTLETT K S MCKEITH F K VANDEHAAR M J | 2006 | Journal of Animal Science2006，84，6： | 1 |
| 16 | Cloned multipotential precursors from the mouse cerebrum require FGF-2,whereas glial restricted precursors are stimulated with either FGF-2 or EGF显示文摘 | KILPATRICK T J BARTLETT P F | 1995 | J Neurosci1995，15，： | 1 |
| 17 | Targeting anthracyclines in early breast cancer: new candidate predictive biomarkers emerge 显示文摘 | Munro A F Cameron D A Bartlett J M S | 2010 | Oncogene2010，29，38： | 1 |
| 18 | Covalent attachment of osmium complexes to glucose oxidase and the application of the resulting modified enzyme in an enzyme switch respinsive to glucose显示文摘 | Bottaglini F Bartlett P N Wang J H | 2000 | Anal Chem2000，72，： | 1 |
| 19 | Effect of moisture condition on concrete core strengths 显示文摘 | BARTLETT F M MAC GREGOR J G | 1994 | ACI Materials Journal1994，91，3： | 1 |
| 20 | Modelling of gas- sensitive conducting polymer devices 显示文摘 | Gardner J W Bartlett P N Pratt K F | 1995 | IEE Proceedings- Circuits Devices and Systems1995，142，5： | 1 |
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