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| 1 | Engineering of Yarrowia lipolytica for production of astaxanthin显示文摘Astaxanthin is a red-colored carotenoid,used as food and feed additive.Astaxanthin is mainly produced by chemical synthesis,however,the process is expensive and synthetic astaxanthin is not approved for human consumption.In this study,we engineered the oleaginous yeast Yarrowia lipolytica for de novo production of astaxanthin by fermentation.First,we screened 12 different Y.lipolytica isolates for β-carotene production by introducing two genes for β-carotene biosynthesis:bi-functional phytoene synthase/lycopene cyclase(crtYB)and phytoene desaturase(crtI)from the red yeast Xanthophyllomyces dendrorhous.The best strain produced 31.1±0.5 mg/L β-carotene.Next,we optimized the activities of 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMG1)and geranylgeranyl diphosphate synthase(GGS1/crtE)in the best producing strain and obtained 453.9±20.2 mg/L β-carotene.Additional downregulation of the competing squalene synthase SQS1 increased the β-carotene titer to 797.1±57.2 mg/L.Then we introduced β-carotene ketolase(crtW)from Paracoccus sp.N81106 and hydroxylase(crtZ)from Pantoea ananatis to convert β-carotene into astaxanthin.The constructed strain accumulated 10.4±0.5 mg/L of astaxanthin but also accumulated astaxanthin biosynthesis intermediates,5.7±0.5 mg/L canthaxanthin,and 35.3±1.8 mg/L echinenone.Finally,we optimized the copy numbers of crtZ and crtW to obtain 3.5 mg/g DCW(54.6 mg/L)of astaxanthin in a microtiter plate cultivation.Our study for the first time reports engineering of Y.lipolytica for the production of astaxanthin.The high astaxanthin content and titer obtained even in a small-scale cultivation demonstrates a strong potential for Y.lipolytica-based fermentation process for astaxanthin production. | Kanchana Rueksomtawin Kildegaard Belen Adiego-Perez David Domenech Belda Jaspreet Kaur Khangura Carina Holkenbrink Irina Borodina | 2017 | Synthetic and Systems Biotechnology2017，2，4： | 12 |
| 2 | How do family ownership, control and management affect firm value?显示文摘 | Belen Villalonga Raphael Amit | 2005 | Journal of Financial Economics2005，，2： | 4 |
| 3 | Nonbismuth Quadruple (Concomitant) Therapy: Empirical and Tailored Efficacy versus Standard Triple Therapy for Clarithromycin‐Susceptible Helicobacter pylori and versus Sequential Therapy for Clarithromycin‐Resistant Strains显示文摘 | Javier Molina‐Infante Carmen Pazos‐Pacheco Gema Vinagre‐Rodriguez Belen Perez‐Gallardo Carmen Due?as‐Sadornil Moisés Hernandez‐Alonso Guadalupe Gonzalez‐Garcia Jose M. Mateos‐Rodriguez Miguel Fernandez‐Bermejo Javier P. Gisbert | 2012 | Helicobacter2012，，4： | 4 |
| 4 | Recent advances in transient imaging:A computer graphics and vision perspective显示文摘Transient imaging has recently made a huge impact in the computer graphics and computer vision fields.By capturing,reconstructing,or simulating light transport at extreme temporal resolutions,researchers have proposed novel techniques to show movies of light in motion,see around corners,detect objects in highly-scattering media,or infer material properties from a distance,to name a few.The key idea is to leverage the wealth of information in the temporal domain at the pico or nanosecond resolution,infor-mation usually lost during the capture-time temporal integration.This paper presents recent advances in this field of transient imaging from a graphics and vision perspective,including capture techniques,analysis,applications and simulation. | Adrian Jarabo Belen Masia Julio Marco Diego Gutierrez | 2017 | Visual Informatics2017，1，1： | 4 |
| 5 | Clinical-pathological and molecular characterization of long-term survivors with advanced non-small cell lung cancer显示文摘Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with chemotherapy.There are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the literature.In this multi-center study,we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular characterization.Methods:In the first step,we conducted a clinical-pathological characterization of the patients.Afterwards,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the samples.Results:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type NSCLC with an ECOG of 0-1.We obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)families.Finally,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(ANCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of NSCLCLS with no driver alterations,obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes. | Juan Moreno-Rubio Santiago Ponce Rosa Alvarez Maria Eugenia Olmedo Sandra Falagan Xabier Mielgo Fatima Navarro Patricia Cruz Luis Cabezon-Gutierrez Carlos Aguado Gonzalo Colmenarejo Marta Munoz-Fernandez de Leglaria Ana Belen Enguita Maria Cebollero Amparo Benito Isabel Alemany Carolina del Castillo Ricardo Ramos Ana Ramirez de Molina Enrique Casado Maria Sereno | 2020 | Cancer Biology & Medicine2020，17，2： | 4 |
| 6 | Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63显示文摘 | Lourdes DIAZ-RODRIGUEZ Olga GARCIA-MARTINEZ Manuel ARROYO-MORALES Belen RUBIO-RUIZ Concepcien RUIZ | 2010 | Acta Pharmacologica Sinica2010，31，11： | 3 |
| 7 | Ownership structureand corporate performance显示文摘 | HAROLD DEMSETZ BELEN VILLALONGA | 2001 | Journal of Corporate Finance2001，，7： | 2 |
| 8 | Automated Deep Learning Empowered Breast Cancer Diagnosis UsingBiomedical Mammogram Images显示文摘Biomedical image processing is a hot research topic which helps to majorly assist the disease diagnostic process.At the same time,breast cancer becomes the deadliest disease among women and can be detected by the use of different imaging techniques.Digital mammograms can be used for the earlier identification and diagnostic of breast cancer to minimize the death rate.But the proper identification of breast cancer has mainly relied on the mammography findings and results to increased false positives.For resolving the issues of false positives of breast cancer diagnosis,this paper presents an automated deep learning based breast cancer diagnosis(ADL-BCD)model using digital mammograms.The goal of the ADL-BCD technique is to properly detect the existence of breast lesions using digital mammograms.The proposed model involves Gaussian filter based pre-processing and Tsallis entropy based image segmentation.In addition,Deep Convolutional Neural Network based Residual Network(ResNet 34)is applied for feature extraction purposes.Specifically,a hyper parameter tuning process using chimp optimization algorithm(COA)is applied to tune the parameters involved in ResNet 34 model.The wavelet neural network(WNN)is used for the classification of digital mammograms for the detection of breast cancer.The ADL-BCD method is evaluated using a benchmark dataset and the results are analyzed under several performance measures.The simulation outcome indicated that the ADL-BCD model outperforms the state of art methods in terms of different measures. | JoséEscorcia-Gutierrez Romany F.Mansour Kelvin Belen Javier Jiménez-Cabas Meglys Pérez Natasha Madera Kevin Velasquez | 2022 | Computers, Materials & Continua2022，，6： | 2 |
| 9 | Antibiotic resistance in non-enterocoecal lactic acid bacteria and bifidobacteria显示文摘 | AMMOR M S BELEN FLOREZ A MAYO B | 2007 | Food Micro- biol2007，24，6： | 1 |
| 10 | Differential effects of fluoxetine and venlafaxine on memory recognition:possible mechanisms of action 显示文摘 | Valeria Paola Carlini Maria Belen Poretti Mathias Rask- Andersen | 2012 | Prog Neuropsychopharmacol Biol Psychiatry2012，38，2： | 1 |
| 11 | Parallcl ization of the Scatter Search for the p median Problem 显示文摘 | FELIX G L BELEN M B JOSE A M P | 2003 | Parallel Computing2003，29，5： | 1 |
| 12 | Age-related changes in the avian primary lymphoid organs (thymus and bursa of Fabricius)显示文摘 | Emilia C Pablo P P Belen D | 2003 | Mierosc Res Techniq2003，62，： | 1 |
| 13 | Polymorphisms in the insulin receptor substrate-1 (IRS-1) gene and the insulin receptor substrate-2 (IRS-2) gene influence glucose homeostasis and body mass index in women with polycystic ovary syndrome and non-hyperandrogenic controls显示文摘 | Gemma V Jose BC Belen R | 2005 | Hum Reprod2005，20，11： | 1 |
| 14 | The Effect of Brand Associations on Consumer Response显示文摘 | RIO A BELEN D RODOLFO V | 2001 | Journal of Consumer Marketing2001，18，5： | 1 |
| 15 | Weather, climate and tourism : a geographical perspective 显示文摘 | M Belen Gomez Martin | 2005 | Annals of tourism research2005，32，3： | 1 |
| 16 | Atmospheric correction of optical imagery from MODIS and Reanalysis atmospheric products显示文摘 | Juan C. Jiménez-Mu?oz José A. Sobrino Cristian Mattar Belen Franch | 2010 | Remote Sensing of Environment2010，，10： | 1 |
| 17 | Ultrasound as a catalyzed of a- queous systemize state of the artand environmental applications显示文摘 | Inee N H Belen R K | 2001 | Appl Catalysis B Envir2001，，29： | 1 |
| 18 | Ownership Structure and Corporate Performance显示文摘 | Demsetz Harold Villalonga Belen | 2001 | Journal of Corporate Finance2001，，7： | 1 |
| 19 | Review on thermal energy storage with phase change:Materials,heat transfer analysis and applications显示文摘 | Belen Zalba Jose M Marin Luisa F Cabeza | 2003 | Applied Thermal Engineering2003，23，3： | 1 |
| 20 | Person-organization Fit, OCB and Performance Appraisal : Evidence from Matched Supervisor-salesperson Data set in a Spanish Context显示文摘 | Vilela Belen Bande | 2008 | Industrial Marketing Management2008，，37： | 1 |
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