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	题名	作者	年代	出处	被引量
1	Molecular diagnostics in a teacup:Non-Instrumented Nucleic Acid Amplification(NINA) for rapid,low cost detection of Salmonella enterica显示文摘We report on the use of a novel non-instrumented platform to enable a Loop Mediated isothermal Amplification(LAMP) based assay for Salmonella enterica.Heat energy is provided by addition of a small amount(<150 g) of boiling water,and the reaction temperature is regulated by storing latent energy at the melting temperature of a lipid-based engineered phase change material.Endpoint classification of the reaction is achieved without opening the reaction tube by observing the fluorescence of sequence-specific FRET-based assimilating probes with a simple handheld fluorometer.At or above 22℃ ambient temperature the non-instrumented devices could maintain reactions above a threshold temperature of 61℃ for over 90 min-significantly longer than the 60 min reaction time.Using the simple format,detection limits were less than 20 genome copies for reactions run at ambient temperatures ranging from 8 to 36℃.When used with a pre-enrichment step and non-instrumented DNA extraction device,trace contaminations of Salmonella in milk close to 1 CFU/mL could be reliably detected.These findings illustrate that the non-instrumented amplification approach is a simple,viable,low-cost alternative for field-based food and agricultural diagnostics or clinical applications in developing countries.	KUBOTA Ryo LABARRE Paul WEIGL Bernhard H LI Yong HAYDOCK Paul JENKINS Daniel M	2013	Chinese Science Bulletin2013，58，10：	4
2	Generation of immunostimulatory dendritic cells from human CD34^+ hematopoietic progenitor cells of the bone marrow and peripheral blood显示文摘	BERNHARD H DISIS M L HEIMFELD S	1995	Cancer Res1995，55，5：	1
3	Granulocyte-macrophage colony-stimulating factor:an effective adjuvant for protein and peptide-basedvaccines显示文摘	DISIS M L BERNHARD H SHIOTA F M	1996	Blood1996，88，1：	1
4	Impact of nutrition on PCB toxicity显示文摘	Zuzana M Elizabeth O Michal T Bernhard H	2007	Environmental Toxicology and Pharmacology2007，25，2：	1
5	Gas evolution at graphite anodes depending on electrolyte water content and SEI quality studied by on-line electrochemical mass spectrometry显示文摘	Bernhard R Metzger M Gasteiger H A	2015	Journal of the Electrochemical Society2015，162，10：	1
6	Method development for the determination of 52 pesticides in tobacco by liquid chromatography-tandem mass spectrometry 显示文摘	Bernhard M H	2009	Journal of Chromatography A2009，1216，：	1
7	Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL-60 cells显示文摘	BERNHARD M K BREITINGER H G	2000	Biochem Biophys Acta2000，1492，：	1
8	Continuous wavelet transform ridge extraction for spectral interferometry imaging显示文摘	LIEBLING M BERNHARD T F BACHMANN A H	2005	Proceeding of SPIE2005，5690，：	1
9	Spectrum Access Technologies: The Past, the Present, andthe Future显示文摘	REED J H BERNHARD J T PARK J M	2012	Proceedings of the IEEE2012，100，：	1
10	Assessing Computer Waste Generation in Chile Using Material Flow Analysis显示文摘	BERNHARD S BONI H SCHLUEP M	2010	Waste Management2010，，3：	1
11	Gas evolution at graphite anodes depending on electrolyte water content and SEI quality studied by on-line electrochemical mass spectrometry显示文摘	Bernhard R Metzger M Gasteiger H A	2015	Journal of the Electrochemical Society2015，162，10：	1
12	Granulocyte-macrophage colony-stimulating factor:an effective adjuvant for protein and peptide-based vaccines显示文摘	Disis M L Bernhard H Shiota F M	1996	Blood1996，88，1：	1
13	Method development for the determination of 52 pesticides in tobacco by liquid chromatography-tandem mass spectrometry 显示文摘	Bernhard M H	2009	Journal of Chromatography A2009，1216，51：	1
14	Weekly high-dose leucovorin versus low-dose leucovorin combined with fluorouracil in advanced colorectal cancer: results of a randomized multicenter trial 显示文摘	Jager E Heike M Bernhard H	1996	J Clin Oncol1996，14，8：	1
15	CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen显示文摘	Davis H L Bernhard H Shiota F M	1998	Immunol1998，160，2：	1
16	Subsidence in intrac- ontinental basins due to dynamic topography 显示文摘	Christian H R Dietmar M Bernhard S	2008	Physics of the Earth and Planetary Interiors2008，171，：	1
17	Method developmentfor the determination of selected pesticides on tobacco by high-performance liquid chromatography electros- pray ionisation-tandem mass spectrometry显示文摘	Bernhard M H Ludwig H J	2008	Talan- ta2008，74，5：	1
18	Assessing Computer Waste Generation in Chile Using Material Flow Analysis显示文摘	Bernhard S Boni H Schluep M	2010	Waste Management2010，，3：	1
19	Granulocyte-maerophage colony-stimulating factor:an effective adjuvant for protein and peptide-based vaccines显示文摘	DISIS M L BERNHARD H SHIOTA F M	1996	Blood1996，88，1：	1
20	Granulocyte-macro- phage colony-stimulating factor: an effective adjuvant for pro- tein and peptide-based vaccines 显示文摘	Disis M L Bernhard H Shiota F M	1996	Blood1996，88，1：	1

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