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| 1 | Inhibition of apoptosis by oncogenic hepatitis B virus X protein: Implications for the treatment of hepatocellular carcinoma显示文摘Hepatitis B virus X protein(HBx) plays an important role in the development of hepatocellular carcinoma(HCC). In addition, hepatoma upregulated protein(HURP) is a cellular oncogene that is upregulated in a majority of HCC cases. We highlight here recent findings demonstrating a link between HBx, HURP and anti-apoptosis effects observed in cisplatin-treated HCC cells. We observed that Hep3B cells overexpressing HBx display increased HURP mRNA and protein levels, and show resistance to cisplatin-induced apoptosis. Knockdown of HURP in HBx-expressing cells reverses this effect, and sensitizes cells to cisplatin. The anti-apoptotic effect of HBx requires activation of the p38/MAPK pathway as well as expression of SATB1, survivin and HURP. Furthermore, silencing of HURP using short-hairpin RNA promotes accumulation of p53 and reduces cell proliferation in SK-Hep-1 cells(p53^(+/–)), whereas these effects are not observed in p53-mutant Mahlavu cells. Similarly, HURP silencing does not affect the proliferation of H1299 lung carcinoma cells or Hep3 B HCC cells which lack p53. Silencing of HURP sensitizes SK-Hep-1 cells to cisplatin. While HURP overexpression promotes p53 ubiquitination and degradation by the proteasome, HURP silencing reverses these effects. Inoculation of SK-Hep-1 cancer cells in which HURP has been silenced produces smaller tumors than control in nude mice. Besides, gankyrin, a positive regulator of the E3 ubiquitin ligase MDM2, is upregulated following HURP expression, and silencing of gankyrin reduces HURP-mediated downregulation of p53. In addition, we observed a positive correlation between HURP and gankyrin protein levels in HCC patients(r^2 = 0.778; n = 9). These findings suggest a role for the viral protein HBx and the host protein HURP in preventing p53-mediated apoptosis during cancer progression and establishment of chemoresistance. | Chuck C K Chao | 2016 | World Journal of Hepatology2016，8，25： | 5 |
| 2 | Medical training fails to prepare providers to care for patients with chronic hepatitis B infection显示文摘AIM: To investigate physicians' knowledge including chronic hepatitis B(CHB) diagnosis, screening, and management in various stages of their training.METHODS: A voluntary 20-question survey was administered in Santa Clara County, CA where Asian and Pacific Islanders(API) account for a third of the population. Among the 219 physician participants,there were 63 interns, 60 second-year residents, 26 chief residents and 70 attending physicians. The survey asked questions regarding respondents' demographics,general hepatitis B virus knowledge questions(i.e.,transmission, prevalence, diagnostic testing, prevention,and treatment options), as well as, self-reported practice behavior and confidence in knowledge.RESULTS: Knowledge about screening and managing patients with CHB was poor: only 24% identified the correct tests to screen for CHB, 13% knew the next steps for patients testing positive for CHB, 18% knew the high prevalence rate among API, and 31% knew how to screen for liver cancer. Wald chi-square analysis determined the effect of training level on knowledge; in all cases except for knowledge of liver cancer screening(p = 0.0032), knowledge did not significantly increase with length in residency training or completion of residency.CONCLUSION: Even in a high-risk region, both medical school and residency training have not adequatelyprepared physicians in the screening and management of CHB. | Stephanie D Chao Bing-Mei Wang Ellen T Chang Li Ma Samuel K So | 2015 | World Journal of Gastroenterology2015，21，22： | 2 |
| 3 | Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures显示文摘Nanomanipulation under scanning electron microscopy(SEM)enables direct interactions of a tool with a sample.We recently developed a nanomanipulation technique for the extraction and identification of DNA contained within sub-nuclear locations of a single cell nucleus.In nanomanipulation of sub-cellular structures,a key step is to identify targets of interest through correlating fluorescence and SEM images.The DNA extraction task must be conducted with low accelerating voltages resulting in low imaging resolutions.This is imposed by the necessity of preserving the biochemical integrity of the sample.Such poor imaging conditions make the identification of nanometer-sized fiducial marks difficult.This paper presents an affine scale-invariant feature transform(ASIFT)based method for correlating SEM images and fluorescence microscopy images.The performance of the image correlation approach under different noise levels and imaging magnifications was quantitatively evaluated.The optimal mean absolute error(MAE)of correlation results is 68634 nm under standard conditions.Compared with manual correlation by skilled operators,the automated correlation approach demonstrates a speed that is higher by an order of magnitude.With the SEM-fluorescence image correlation approach,targeted DNA was successfully extracted via nanomanipulation under SEM conditions. | Zheng Gong Brandon K Chen Jun Liu Chao Zhou Dave Anchel Xiao Li Ji Ge David P Bazett-Jones Yu Sun | 2014 | Light(Science & Applications)2014，3，1： | 2 |
| 4 | Removal of mixed heavy metal ions in wastewater by zeolite 4A and residual products from recycled coal fly ash 显示文摘 | Hui K S Chao C Y H Kot S C | 2005 | Journal of Hazardous Materials2005，127，13： | 1 |
| 5 | Hardware efficient fast parallel FIR filter structures based on iterated short convolution 显示文摘 | Cheng Chao Keshab K Parhi | 2004 | IEEE Trans on Circuits and Systems-I: Regular Papers2004，51，8： | 1 |
| 6 | Non-planar dynamic modeling for the optical disk drive spindles equipped with an automatic balancer显示文摘 | Chao P C Huang Y D Sung C K | 2003 | Mechanism and Machine Theory2003，38，： | 1 |
| 7 | Chinese purchasing managers preferences in supplier selection显示文摘 | Mummalaneni V Dubas K M Chao C | 1996 | Industrial Marketing Management1996，25，2： | 1 |
| 8 | Effects of fertilization promoting peptide,adenosine,and pentoxifylline on thawed human sperm显示文摘 | Huang P T Chen S U Chao K H | 2003 | Arch Androl2003，49，2： | 1 |
| 9 | Numerical simulation of transient temperature and residual stresses in friction stir welding of 304Lstainless steel显示文摘 | Zhu X K Chao Y J | 2004 | Journal of Materials Processing Technology2004，146，2： | 1 |
| 10 | Development of muhispectral image processing algorithms for identification of wholesome, septicemic, and inflammatory process chickens 显示文摘 | Yang C Chao K Chen Y | 2005 | Journal of Food Engineering2005，69，2： | 1 |
| 11 | TIP30 deficiency in- creases susceptibility to tumorigeneis显示文摘 | Mitsuhiro I Chao J Kristy K | 2003 | Cancer Reacher2003，63，24： | 1 |
| 12 | Further complexity reduction of parallel FIR filters显示文摘 | Cheng Chao Keshab K Parhi | 2005 | IEEE International Symposium on Circuits and Systems2005，2，： | 1 |
| 13 | Effects of step - change of synthesis temperature on synthesis of zeolite 4A from coal fly ash 显示文摘 | HUI K S CHAO C Y H | 2006 | Microporous and Mesoporous Materials2006，88，2： | 1 |
| 14 | R-curve behavior and flaw insensitivity of Ce-TZP/Al2O3 composite显示文摘 | Ramachandran N Chao L Y Shetty D K | | Journal of the American Ceramic Society0，176，4： | 1 |
| 15 | Characterization of crack-tip field and constraint for bending specimens under large-scale yielding显示文摘 | Chao Y J Zhu X K Kim Y | 2004 | International Journal of Fracture2004，127，： | 1 |
| 16 | Detection of defects on selected apple cultivars using hyperspectral and multispectral image analysis显示文摘 | Mehl P M Chao K Kim M | 2002 | Applied Engineering in Agriculture2002，18，2： | 1 |
| 17 | Stopping rules and estimation for recapture debugging with unequal failure rates 显示文摘 | CHAO A MA M C YANG M C K | 1993 | Biometrika1993，80，1： | 1 |
| 18 | Hydrogen bonding with adsorbent during storage governs drug dissolution from solid-dispersion grannules显示文摘 | Manish K Gupta Yin - Chao Tseng David Goldman | 2002 | Pharmaceutical Research2002，19，11： | 1 |
| 19 | A pro-drug of the green tea polyphenol (-)-epigallocatechin-3-gallate(EGCG) prevents differenti- ated SH-SY5Y cells from toxicity induced by 6-hydroxydopamine显示文摘 | CHAO Jianfei LAU W K W HUIE M J | 2010 | Neuroseience Letters2010，469，3： | 1 |
| 20 | Water-to-air mass transfer of VOCs: Lahoratory-scale air sparging system显示文摘 | Chao K P Ong S K Protopapas A | 1998 | Journal of Envi?ronmental Engineering1998，124，11： | 1 |
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