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| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | BCL-2 antisense therapy in patients with non-Hodgkin lymphoma 显示文摘 | Webb A Cunningham D Cotter F | 1997 | lancet1997，349，9059： | 1 |
| 3 | Surviving the acid test : responses of gram-positive bacteria to low pH 显示文摘 | Cotter P D Hill C | 2003 | Microbiol Mol Biol Rev2003，67，3： | 1 |
| 4 | Bacteriocins: developing innate immunity for food显示文摘 | Cotter P D Hill C Ross R P | 2005 | Nat Rev Microbiol2005，3，： | 1 |
| 5 | High-throughput sequence-based analysis of the bacterial com-position of kefir and an associated kefir grain显示文摘 | Dobson A O'Sullivan O Cotter P D | 2011 | FEMSMicrobiology Letters2011，320，： | 1 |
| 6 | A randomized clinical tri- al of vision therapy/orthoptics versus pencil pushups for the treat- ment of convergence insufficiency in young adults 显示文摘 | Scheiman M Mitchell GL Cotter S et d | 2005 | Optom Vis Sci2005，82，7： | 1 |
| 7 | Waterborne outbreak of Norwalk-like virus gastroenteritis at a tourist resort,Italy显示文摘 | BOCCIA D TOZZI A COTTER B | | 0，，06： | 1 |
| 8 | Sparse channel estimation via matching pursuit with application to equalization显示文摘 | Cotter S F Rao B D | 2002 | Communications IEEE Transactions on2002，50，3： | 1 |
| 9 | Bio - MOS modulates kinetics of phytohemagglutinin wattle reaction in chickens 显示文摘 | Cotter P F Weinner J D | 1997 | Poultry Sci1997，76，1： | 1 |
| 10 | Bacteriocins:developing innate immunity for food显示文摘 | Paul D Cotter Colin Hill Paul Ross R | 2005 | Nature2005，，3： | 1 |
| 11 | Sparse channel estimation via matching pursuit with application to equalization显示文摘 | COTTER S F RAO B D | 2002 | IEEE Trans Commun2002，50，3： | 1 |
| 12 | Sparse channel estimation via matching pursuit with application to equalization 显示文摘 | Cotter S F Rao B D | 2002 | IEEE Trans on Communication2002，50，3： | 1 |
| 13 | Bacteriocins:developinginnate immunity for food显示文摘 | COTTER P D HILL C ROSS R P | 2005 | Nat Rev Microbiol2005，3，10： | 1 |
| 14 | BCL-2 antisense therapy in patients with non-Hodgkin lymphoma显示文摘 | Webb A Cunningham D Cotter F | 1997 | Lancet1997，349，9059： | 1 |
| 15 | Disturbance of Notch-1 and Wnt signalling proteins in neuroglial balloon cells and abnormal large neurons in focal cortical dysplasia in human cortex 显示文摘 | Cotter D Honavar M Lovestone S | 1999 | Acta Neuropathol ( Berl)1999，98，5： | 1 |
| 16 | Subset selection innoise based on diversity measure minimization 显示文摘 | Rao B D Engan K Cotter S F | 2003 | IEEETransactions on Signal Processing2003，51，3： | 1 |
| 17 | The use of zolpidem in the treatment of progressive supranuelear palsy显示文摘 | Cotter C Armytage T Crimmins D | 2010 | J Clin Neurosci2010，7，3： | 1 |
| 18 | Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH 显示文摘 | COTTER P D HILL C | 2003 | Microbiology and Molecular Biology Reviews2003，67，3： | 1 |
| 19 | Sparse Solution to Linear Inverse Problems with Multiple Measurement Vectors显示文摘 | Cotter S F Rao B D Engan K | 2005 | IEEE Transcations on Signal Processing2005，53，7： | 1 |
| 20 | Sparse channel estimation via matc- hing pursuit with application to equalization 显示文摘 | Cotter S F Rao B D | 2002 | IEEE Transactions on Communications2002，50，3： | 1 |
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