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| 1 | 哮喘-慢性阻塞性肺疾病重叠的研究进展显示文摘支气管哮喘和慢性阻塞性肺疾病(COPD)是2种不同的慢性气道炎性反应性疾病,两者在病因、发病年龄、病理生理、治疗和预后方面存在差异。然而,临床发现部分具有慢性气道炎性疾病的患者可同时具有以上2种疾病的特点,临床多将其描述为哮喘一慢性阻塞性肺疾病重叠综合征(ACOS)。2017全球哮喘防治创议(GINA)将其描述为哮喘一慢性阻塞性肺疾病重叠(ACO)。已有的研究发现,ACO比单独的哮喘和慢性阻塞性肺疾病急性加重更加频繁,生活质量更差,肺功能下降更快及病死率更高,消耗更多医疗资源,而目前对于ACO的认识还处于一个初级阶段,相关研究也逐渐受到广泛关注,文章对哮喘一慢性阻塞性肺疾病重叠近年的研究进展进行阐述。 | 檀春玲 王桦 ZHANG Guifen LI Chenfang LIU Guodong | 2018 | 疑难病杂志2018，17，6： | 13 |
| 2 | Angular response of ‘pin-hole' imaging structure measured by collimated β source显示文摘The pitch-angle distribution of energetic particles is important for space physics studies on magnetic storm and particle acceleration.A‘pin-hole’imaging structure is built with the‘pin-hole’technique and a position sensitive detector,which can be used to measure the pitch angle distribution of energetic particles.To calibrate the angular response of the‘pin-hole’imaging structure,special experiment facilities are needed,such as the particle accelerator with special design.The features of this kind of particle accelerator are:1)The energy range of the outgoing particles should be mid-energy particles(tens keV to several hundred keV);2)the particle flux should be consistent in time-scale;3)the directions of the outgoing particles should be the same and 4)the particle number within the spot should be low enough.In this paper,a method to calibrate the angular response of the‘pin-hole’imaging structure by the90Sr/90Y β source with a collimator is introduced and simulated by Geant4 software.The result of the calibration with the collimated β source is in accord with the Geant4 simulations,which verifies the validity of this method. | ZOU Hong LUO Lin LI ChenFang JIA XiangHong XU Feng CHEN HongFei CHEN Jiang SHI WeiHong YU XiangQian ZOU JiQing | 2013 | Science China(Technological Sciences)2013，56，11： | 10 |
| 3 | effective treatment of post-intubation subglottic stenosis in children with holmium laser therapy and cryotherapy via flexible bronchoscopy显示文摘Importance:Post-intubation subglottic stenosis(SGS)in children can be life threatening.Definitive treatment varies and lacks a universally accepted approach.Objective:We performed a prospective study to assess the safety and feasibility of holmium laser combined with cryotherapy delivered via flexible bronchoscopy for the treatment of post-intubation SGS in children.Methods:This study involved all patients with post-intubation SGS seen at the Interventional Pulmonology Department of Beijing Children's Hospital between July 2014 and December 2016.Holmium laser treatment and cryotherapy was then performed under flexible bronchoscopy,whose parents refused to accept the alternative standard treatment of tracheotomy and balloon dilation under direct laryngoscopy.results:Sixteen patients with post-intubation SGS were included in this study.Ages ranged from 2 months to 12.25 years old.According to the Cotton-Myer grading system,three cases were Grade II,12 cases were Grade III,and one case was Grade IV.According to the McCaffrey system,eight cases were Stage 1,two cases were Stage 2,and six cases were Stage 3.The average number of procedures was 4.88.Fifteen of the 16 patients achieved clinical cure.One patient achieved clinical improvement.The average treatment course duration was 55.31 days.No severe complications were seen.Post-treatment clinical symptoms,endoscopic findings and quality of life showed marked improvement.Interpretation:Our study supports the conclusion that holmium laser treatment combined with cryotherapy via flexible bronchoscopy appears to be a safe and feasible treatment for post-intubation SGS in children. | Anxia Jiao Fang Liu Andrew D.Lerner Xiaochun Rao Yan Guo Chenfang Meng Yuena Pan Gan Li Zheng Li Fang Wang Jing Zhao Yuyan Ma Xicheng Liu Xin Ni Kunling Shen | 2019 | Pediatric Investigation2019，3，1： | 10 |
| 4 | Gene expression regulators——MicroRNAs显示文摘A large class of non-coding RNAs found in small molecule RNAs are closely associated with the regula-tion of gene expression, which are called microRNA (miRNA). MiRNAs are coded in intergenic or intronic regions and can be formed into foldback hairpin RNAs. These transcripts are cleaved by Dicer, generating mature miRNAs that can silence their target genes in different modes of action. Now, research on small molecule RNAs has gotten breakthrough advance in biology. To discover miRNA genes and their target genes has become hot topics in RNA research. This review attempts to look back the history of miRNA discovery, to introduce the methods of screening miRNAs, to localize miRNA loci in genome, to seek miRNA target genes and the biological func-tion, and to discuss the working mechanisms of miRNAs. Finally, we will discuss the potential important roles of miRNAs in modulating the genesis, development, growth, and differentiation of organisms. Thus, it can be predicted that a complete understanding of miRNA functions will bring us some new concepts, approaches and strategies for the study of living beings. | CHENFang YINQ.James | 2005 | Chinese Science Bulletin2005，50，13： | 7 |
| 5 | Metabolic reprogramming in triple-negative breast cancer显示文摘Since triple-negative breast cancer(TNBC)was first defined over a decade ago,increasing studies have focused on its genetic and molecular characteristics.Patients diagnosed with TNBC,compared to those diagnosed with other breast cancer subtypes,have relatively poor outcomes due to high tumor aggressiveness and lack of targeted treatment.Metabolic reprogramming,an emerging hallmark of cancer,is hijacked by TNBC to fulfill bioenergetic and biosynthetic demands;maintain the redox balance;and further promote oncogenic signaling,cell proliferation,and metastasis.Understanding the mechanisms of metabolic remodeling may guide the design of metabolic strategies for the effective intervention of TNBC.Here,we review the metabolic reprogramming of glycolysis,oxidative phosphorylation,amino acid metabolism,lipid metabolism,and other branched pathways in TNBC and explore opportunities for new biomarkers,imaging modalities,and metabolically targeted therapies. | Zhanyu Wang Qianjin Jiang Chenfang Dong | 2020 | Cancer Biology & Medicine2020，17，1： | 5 |
| 6 | Non-anthracycline-containing docetaxel and cyclophosphamide regimen is associated with sustained worse outcome compared with docetaxel, anthracycline and cyclophosphamide in neoadjuvant treatment of triple negative and HER2-positive breast cancer patients:显示文摘Objective: A previous study demonstrated that non-anthracycline-containing docetaxel plus cyclophosphamide(TC) regimen was inferior to docetaxel, anthracycline and cyclophosphamide(TAC) in neoadjuvant treatment of triple-negative breast cancer(TNBC) and human epidermal growth factor receptor-2-(HER2)-positive breast cancer in a short-term follow-up. Herein, long-term follow-up survival outcomes have been investigated.Methods: TNBC or HER2-positive patients were randomized to receive 6 cycles of TC or TAC neoadjuvant treatment. The primary endpoint was pathological complete remission(p CR). Secondary endpoints included clinical response rate, event-free survival(EFS), and overall survival(OS).Results: A cohort of 96 patients consisted of 45 in TC and 51 in TAC arm. With a median follow-up period of53(range, 8–76) months, the patients achieving p CR post neoadjuvant chemotherapy exhibited superior EFS and OS than patients without p CR(P<0.05). TAC treatment resulted in consistently better EFS than TC treatment:the estimated 5-year EFS was 66.1% vs. 29.8%(P=0.002). Moreover, the estimated 5-year OS was also in favor of TAC: 88.4% vs. 51.6%(P<0.001). Multivariable analysis demonstrated that the treatment regimen was an independent prognostic factor, and patients treated with TAC had a superior EFS [hazard ratio(HR), 0.48; 95%confidence interval(95% CI), 0.26–0.90; P=0.021] and OS(HR, 0.20; 95% CI, 0.08–0.60; P=0.003).Conclusions: The updated long-term follow-up data demonstrated a sustained benefit in EFS and OS from anthracycline-containing TAC treatment, indicating that anthracycline is an essential and effective drug in this clinical trial. | Xiaosong Chen Guolin Ye Chenfang Zhang Xinzheng Li Kunwei Shen | 2016 | Chinese Journal of Cancer Research2016，28，6： | 4 |
| 7 | Casein kinase Ⅱ interacts with prion protein in vitro and forms complex with native prion protein in vivo显示文摘最必要、关键的步在能递送的海绵状的 encephalopathy 的致病期间是到病理学的 isoform 的细胞的 prion 蛋白质的 conformational 变化。酷蛋白 kinase II (CK2 ) 是一无所不在地表示了并且 evolutionarily 保存了为生存能力是必要的多种的蛋白质 kinase。探索在 CK2 和 prion 蛋白质(PrP ) 之间的可能的分子的相互作用,人的 CK2α 和 CK2β 的全身的序列互补 DNA 作为模板从房间线 SH-SY5Y 用彻底的送信人 RNA 与反向的抄写聚合酶链反应被放大;然后,熔化蛋白质 histidine-CK2α 和谷胱甘肽 S-transferase-histidine-CK2β 在 Escherichia 关口 i 被表示。在 CK2 和 PrP 之间的相互作用与 co-immunoprecipitation 和下拉试金被评估。结果证明 recombinant PrP 与 CK2α ,然而并非与 CK2β 明确地跳了。在仓鼠大脑的本国的 CK2 和 PrP 与对方一起交往了,形成蛋白质建筑群。从正常大脑的 PrP (diglycosylated, monoglycosylated 和 unglycosylated PrP ) 的三种不同 glycosylated 形式与 CK2α 子单元交往了,尽管 unglycosylated PrP 似乎与 CK2α 子单元有一个更强壮的有约束力的能力。负责与 CK2α 交往的领域在 PrP 的 C 终端片断被定位(残余 91 231 ) 。这研究为在 PrP 和 CK2α 之间的分子的相互作用建议了可靠试验性的数据(两个在 recombinant 和本国的范畴) ,为进一步估计 PrP-CK2 相互作用的潜在的生物意义的科学线索,和在 prion 疾病的致病的 CK2 的可能的角色。 | Jianming Chen Chen Gao Qi Shi Guirong Wang Yanjun Lei Bing Shan Baoyun Zhang Chenfang Dong SongShi Xin Wang Chan Tian Jun Han Xiaoping Dong | 2008 | Acta Biochimica et Biophysica Sinica2008，40，12： | 3 |
| 8 | AVP(4-8) Improves Cognitive Behaviors and Hippocampal Synaptic Plasticity in the APP/PS1 Mouse Model of Alzheimer’s Disease显示文摘Memory deficits with aging are related to the neurodegeneration in the brain, including a reduction in arginine vasopressin(AVP) in the brain of patients with Alzheimer’s disease(AD). AVP(4-8), different from its precursor AVP, plays memory enhancement roles in the CNS without peripheral side-effects. However, it is not clear whether AVP(4-8) can improve cognitive behaviors and synaptic plasticity in the APP/PS1 mouse model of AD. Here, we investigated for the first time the neuroprotective effects of AVP(4-8) on memory behaviors and in vivo long-term potentiation(LTP) in APP/PS1-AD mice.The results showed that:(1) APP/PS1-AD mice had lower spontaneous alternation in the Y-maze than wild-type(WT) mice, and this was significantly reversed by AVP(4-8);(2) the prolonged escape latency of APP/PS1-AD mice in the Morris water maze was significantly decreased by AVP(4-8), and the decreased swimming time in target quadrant recovered significantly after AVP(4-8) treatment;(3) in vivo hippocampal LTP induced by high-frequency stimulation had a significant deficit in the AD mice, and this was partly rescued by AVP(4-8);(4) AVP(4-8)significantly up-regulated the expression levels of postsynaptic density 95(PSD95) and nerve growth factor(NGF)in the hippocampus of AD mice. These results reveal the beneficial effects of AVP(4-8) in APP/PS1-AD mice,showing that the intranasal administration of AVP(4-8)effectively improved the working memory and long-term spatial memory of APP/PS1-AD mice, which may be associated with the elevation of PSD95 and NGF levels in the brain and the maintenance of hippocampal synaptic plasticity. | Xiumin Zhang Fang Zhao Chenfang Wang Jun Zhang Yu Bai Fang Zhou Zhaojun Wang Meina Wu Wei Yang Junhong Guo Jinshun Qi | 2020 | Neuroscience Bulletin2020，36，3： | 3 |
| 9 | PrP mutants with different numbers of octarepeat sequences are more susceptible to the oxidative stress显示文摘One of the physiological functions of cellular prion protein(PrP C )is believed to work as a cellular resistance to oxidative stress,in which the octarepeats region within PrP plays an important role.However,the detailed mechanism is less clear.In this study,the expressing plasmids of wild-type PrP (PrP-PG5)and various PrP mutants containing 0(PrP-PG0),9(PrP-PG9)and 12(PrP-PG12)octarepeats were generated and PrP proteins were expressed both in E.coli and in mammalian cells.Protein aggregation and formation of carbonyl groups were clearly seen in the recombinant PrPs expressed from E.coli after treatment of H2O2.MTT and trypan blue staining assays revealed that the cells expressing the mutated PrPs within octarepeats are less viable than the cells expressing wild-type PrP.Statistically significant high levels of intracellular free radicals and low levels of glutathione peroxidase were observed in the cells transfected with plasmids containing deleted or inserted octarepeats.Remarkably more productions of carbonyl groups were detected in the cells expressing PrPs with deleted and inserted octarepeats after exposing to H2O2.Furthermore,cells expressing wild-type PrP showed stronger resistant activity to the challenge of H2O2 at certain extent than the mutated PrPs and mock. These data provided the evidences that the octarepeats number within PrP is critical for maintaining its activity of antioxidation.Loss of its protective function against oxidative stress may be one of the possible pathways for the mutated PrPs to involve in the pathogenesis of familial Creutzfeldt-Jacob diseases. | AN Run1 ,2,DONG ChenFang 2,LEI YanJun 1,2,HAN Lu 2,LI Ping 2,CHEN JianMing 2,WANG GuiRong 2, SHI Qi 2 ,GAO Chen 2 ,JIANG HuiYing 2 ,ZHOU Wei 2 ,HAN Jun 2 ,CHU YongLie 1 ,DONG XiaoPing 2 1School of Medicine,Xi’an JiaoTong University,Xi’an 710061,China 2State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention,YingXin Rd 100,Beijing 100052,China | 2008 | Science China(Life Sciences)2008，51，7： | 3 |
| 10 | Doppel-induced cytotoxicity in human neuronal SH-SY5Y cells is antagonized by the prion protein显示文摘Doppel (Dpl ) 由于结构、生物化学的类似是象蛋白质一样的 prion (PrP ) ;然而, Dpl 和 PrP 的自然功能仍然保持不清楚。在这研究,编码 Dpl 蛋白质的一个 531-bp 人 PRND 基因序列从人的外部血白细胞被放大。全身、各种各样的截断的人的 Dpl 和 PrP 蛋白质从 Escherichia 被表示并且净化 coli。全身的 Dpl 的补充导致了显著 cytotoxicity,和负责的区域到人的 neuroblastoma 房间 SH-SY5Y 上因为它的 cytotoxicity 在 Dpl 的中间的片断被印射[氨基酸(aa ) 81122 ] 。有趣地,导致 Dpl 的 cytotoxicity 被全身的野类型的 PrP 的存在反对。PrP 异种的碎片上的分析证明 N 终端碎裂(aa 2390 ) PrP 为保护的活动负责。有 Dpl 在 SH-SY5Y 房间上导致了象 Dpl 一样 cytotoxicity 的类似的第二等的结构的截断的 PrP (PrP32121 ) 。而且,铜离子的绑定能在导致 Dpl 的 cytotoxicity 上提高 PrP 的反对效果。Apoptosis 试金表明 Dpl 导致的 cytotoxicity 通过 apoptotic 机制发生了。这些结果建议 Dpl 的功能对 PrP 而非 synergistic 对抗。 | Ping Li Chenfang Dong Yanjun Lei Bing Shan Xinli Xiao Huiying Jiang Xin Wang Chen Gao Qi Shi Kun Xu Chan Tian Jun Han Xiaoping Dong | 2009 | Acta Biochimica et Biophysica Sinica2009，41，1： | 3 |
| 11 | Metabolic reprogram associated with epithelial-mesenchymal transition in tumor progression and metastasis显示文摘Epithelial-mesenchymal Transition(EMT)is a de-differentiation program that imparts tumor cells with the phenotypic and cellular plasticity required for drug resistance,metastasis,and recurrence.This dynamic and reversible events is governed by a network of EMT-transcription factors(EMT-TFs)through epigenetic regulation.Many chromatin modifying-enzymes utilize metabolic intermediates as cofactors or substrates;this suggests that EMT is subjected to the metabolic regulation.Conversely,EMT rewires metabolic program to accommodate cellular changes during EMT.Here we summarize the latest findings regarding the epigenetic regulation of EMT,and discuss the mutual interactions among metabolism,epigenetic regulation,and EMT.Finally,we provide perspectives of how this interplay contributes to cellular plasticity,which may result in the clinical manifestation of tumor heterogeneity. | Yifan Wang Chenfang Dong Binhua P.Zhou | 2020 | Genes & Diseases2020，7，2： | 3 |
| 12 | An analysis of the correlation between the fluxes of high-energy electrons and low-middle-energy electrons in the magnetosphere显示文摘The variation of the flux of energetic electrons in the magnetosphere has been proven to be strongly related to the solar wind speed. Observations of GEO orbit show that the flux of low-energy electrons is not only modulated by the solar wind speed, but, if a time delay is added, is also positively correlated to the flux of high-energy electrons. This feature provides a possible method to forecast the flux of high-energy electrons in GEO orbit. In this study, the correlations of the fluxes between the high-energy electrons and low-middle-energy electrons obtained at different L values and in different orbits are investigated to develop the application of this feature. Based on the analysis of long–term data observed by NOAA POES and GOES, the correlations between the fluxes of high-energy electrons and low–middle–energy electrons are good enough at different L values and in different orbits in quiet time, but this correlation is strongly affected by CME–driven geomagnetic storms. | LI ChenFang ZOU Hong ZONG QiuGang JIA XiangHong CHEN HongFei SHI WeiHong YU XiangQian | 2016 | Science China(Technological Sciences)2016，59，7： | 2 |
| 13 | Stepwise inter-frame correlation-based steganalysis system for video streams 显示文摘 | Liu Bin Liu Fenlin Yang Chenfang | 2008 | Security and Communication networks2008，1，6： | 1 |
| 14 | Air-fuel ratio control in spark-ignition engines using estimation theory 显示文摘 | Chang Chenfang Fekete N P Amstutz A | 1995 | IEEE Transactions on Control Systems Technology1995，13，1： | 1 |
| 15 | The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy显示文摘 | JIN Erhui WANG Chenfang HU Qianqian | 2014 | Romanian Journal of Morphology and Embryology2014，55，4： | 1 |
| 16 | Sevoflurane ameliorates intestinal ischemia - reperfusion - induced lung injury by inhibiting the synergistic action between mast cell activation and oxidative stress 显示文摘 | Luo Chenfang Yuan Dongdong Zhao Weicheng | 2015 | Molecular Medicine Reports2015，12，1： | 1 |
| 17 | Organic thin-film transistors and related devices in life and health monitoring显示文摘The early determination of disease-related biomarkers can significantly improve the survival rate of patients.Thus,a series of explorations for new diagnosis technologies,such as optical and electrochemical methods,have been devoted to life and health monitoring.Organic thin-film transistor(OTFT),as a state-of-the-art nano-sensing technology,has attracted significant attention from construction to application owing to the merits of being label-free,low-cost,facial,and rapid detection with multi-parameter responses.Nevertheless,interference from non-specific adsorption is inevitable in complex biological samples such as body liquid and exhaled gas,so the reliability and accuracy of the biosensor need to be further improved while ensuring sensitivity,selectivity,and stability.Herein,we overviewed the composition,mechanism,and construction strategies of OTFTs for the practical determination of disease-related biomarkers in both body fluids and exhaled gas.The results show that the realization of bio-inspired applications will come true with the rapid development of high-effective OTFTs and related devices. | Chenfang Sun Tie Wang | 2024 | Nano Research2024，17，2： | 1 |
| 18 | Tunable multiwavelength fiber laser based on a polarizationmaintaining erbiumdoped fiber and a polarizationcontroller显示文摘 | Zhang Chenfang Liu Lisong Liu Zhimin | 2011 | Optics Communications2011，284，1011： | 1 |
| 19 | 显示文摘 | WUTing(吴婷) CHANGLin(常琳) FANGBi-yun(方碧云) LIUMin(刘敏) CHENFang(陈芳) | | 分析化学0，29，1： | 1 |
| 20 | COa interacts with Snail and is critical for Snail-mediated E-cadherin repression in human breast cancer 显示文摘 | Chenfang Dong Yadi Wu Jun Yao | 2012 | The Joumal of Clinical Investigation2012，122，4： | 1 |
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