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| 1 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 2 | Polyamines reduces lipid peroxidation induced by different pro-oxidant agents显示文摘 | Nádia Aléssio Velloso Bellé Gerusa Duarte Dalmolin Graciela Fonini Maribel Antonello Rubin Jo?o Batista Teixeira Rocha | 2004 | Brain Research2004，，2： | 3 |
| 3 | Epidemiology of upper gastrointestinal symptoms in Brazil(Epi Gastro):A population-based study according to sex and age group显示文摘AIM:To determine the prevalences of symptoms consistent with gastroesophageal reflux disease(GERD)and dyspepsia in South America.METHODS:A telephone survey was conducted among adult owners of land-based telephones in S?o Paulo,Brazil,using previously validated computer-assisted sampling and survey protocols.The Portugueselanguage survey included(1)sociodemographic characteristics(e.g.,weight,height,smoking)and comorbidities;(2)dietary habits;(3)presence of symptoms consistent with GERD or dyspepsia within the prior 3mo;and(4)use of medications and other therapies to manage symptoms.Data were stratified post-hoc into three homogeneous geographical regions of S?o Paulo according to the Social Exclusion Indices of the districts and postal codes.Survey response data from each respondent were weighted by the numbers of adults and landline telephones in each household.The analyses were weighted to account for sampling design and to be representative of the S?o Paulo population according to city census data.RESULTS:Among 4570 households contacted,an adult from 3050(66.7%)agreed to participate.The nonresponse rate was 33.3%.The mean(SE)respondent age was 42.6(16.0)years.More than half of all respondents were women(53.1%),aged 18 through 49 years(66.7%),married or cohabitating(52.5%),and/or above normal-weight standards(i.e.,35.3%overweight and 16.3%obese).A total of 26.5%of women were perimenopausal.More than 20%of respondents reported highly frequent symptoms consistent with GERD(e.g.,gastric burning sensation=20.8%)or dyspepsia(e.g.,abdominal swelling/distension=20.9%)at least once per month.Prevalences of these symptoms were significantly(approximately 1.5-to 2.0-fold)higher among women than men but did not vary significantly as a function of advancing age.For instance,14.1%of women reported that they experienced stomach burning(symptom of GERD)at least twice per week,compared to 8.4%of men(P=0.012 byχ2 test).A total of 15.7%of women reported that they experienced abdominal swelling(symptom of dyspepsia)at least twice per week,compared to 6.4%of men(P<0.001byχ2 test).Despite frequent manifestations of GERD or dyspepsia,most(≥90%)respondents reported that they neither received prescription medications fromphysicians,nor took behavioral measures(e.g.,dietary modifications),to manage symptoms.CONCLUSION:Symptoms consistent with dyspepsia and GERD are prevalent in Brazil and represent major public-health and clinical challenges. | Maria do Rosário Dias de Oliveira Latorre Aline Medeiros da Silva Décio Chinzon Jaime N Eisig Telma RP Dias-Bastos | 2014 | World Journal of Gastroenterology2014，20，46： | 2 |
| 4 | Reduced semen quality in patients with testicular cancer seminoma is associated with alterations in the expression of sperm proteins显示文摘Testicular cancer seminoma is one of the most common types of cancer among men of reproductive age.Patients with this condition usually present reduced semen quality,even before initiating cancer therapy.However,the underlying mechanisms by which testicular cancer seminoma affects male fertility are largely unknown.The aim of this study was to investigate alterations in the sperm proteome of men with seminoma undergoing sperm banking before starting cancer therapy,in comparison to healthy proven fertile men(control group).A routine semen analysis was conducted before cryopreservation of the samples(n=15 per group).Men with seminoma showed a decrease in sperm motility(P=0.019),total motile count(P=0.001),concentration(P=0.003),and total sperm count(P=0.001).Quantitative proteomic analysis identified 393 differentially expressed proteins between the study groups.Ten proteins involved in spermatogenesis,sperm function,binding of sperm to the oocyte,and fertilization were selected for validation by western blot.We confirmed the underexpression of heat shock-related 70 kDa protein 2(P=0.041),ubiquinol-cytochrome C reductase core protein 2(P=0.026),and testis-specific sodium/potassium-transporting ATPase subunit alpha-4(P=0.016),as well as the overexpression of angiotensin I converting enzyme(P=0.005)in the seminoma group.The altered expression levels of these proteins are associated with spermatogenesis dysfunction,reduced sperm kinematics and motility,failure in capacitation and fertilization.The findings of this study may explain the decrease in the fertilizing ability of men with seminoma before starting cancer therapy. | Tania R Dias Ashok Agarwal Peter N Pushparaj Gulfam Ahmad Rakesh Sharma | 2020 | Asian Journal of Andrology2020，22，1： | 2 |
| 5 | Helicobacter pylori Induces Increased Expression of Toll‐Like Receptors and Decreased Toll‐Interacting Protein in Gastric Mucosa that Persists Throughout Gastric Carcinogenesis显示文摘 | Pedro Pimentel‐Nunes Nádia Gon?alves Inês Boal‐Carvalho Luís Afonso Paula Lopes Roberto Roncon‐Albuquerque Rui Henrique Luís Moreira‐Dias Adelino F. Leite‐Moreira Mário Dinis‐Ribeiro | 2012 | Helicobacter2012，，1： | 2 |
| 6 | Hydrophobic, highly conductive ambient-temperature molten salts显示文摘 | Bomhote P Dias A P Papageorgiou N | 1996 | Inorg Chem1996，35，： | 1 |
| 7 | Rapid silver staining and recover of PCR products separated in polyacrylamide gels显示文摘 | SANGUINETTI C J DIAS N E SIMPSON A J G | 1994 | Bio techniques1994，17，： | 1 |
| 8 | Measurement of the angular acceleration using a PVDF and a piezo-composite显示文摘 | Marat-Mendes Dias C J Marat-Mendes J N | | 0，，： | 1 |
| 9 | Availability of phosphorus in a Brazilian Oxisol cultivated with Eucalyptus after nine years as influenced by phosphorus-fertilizer source,rate and placement显示文摘 | DIAS L E FERNANDEZ J Q P BARROS N F | 2001 | Communications in soil science and plant analysis2001，32，78： | 1 |
| 10 | ATR-FTIR: studies of poly ( propylene oxide)/polybutadiene hi-soft segment urethane/urea membranes 显示文摘 | Queiroz D P De Pinho M N Dias C | 2003 | Macromolecules2003，36，11： | 1 |
| 11 | Value of frational flow reserve in the management of patients with moderate coronary stenosis 显示文摘 | Dias CC Mateus P Bettencourt N | 2004 | Rev Port Cardil2004，23，11： | 1 |
| 12 | Management of displaced fractures of the waist of the seaphoid: meta-analyses of comparative studies显示文摘 | Singh HP Taub N Dias JJ | 2012 | Injury2012，43，6： | 1 |
| 13 | Dealing with imprecise information in group multicriteria decisions: a methodology and a GDSS architechture显示文摘 | Dias L C Cl'maco J N | 2005 | European Journal of Operational Research2005，160，2： | 1 |
| 14 | Seawater Sr isotope variation over the past 300 kyr and influence of global climate cycles 显示文摘 | Dia A N Cohen A S O'Nions R K | 1992 | Nature1992，356，： | 1 |
| 15 | Ultrasound-guided retrograde intubation显示文摘 | Vieira D Lages N Dias J | 2013 | Anaesthesia2013，68，10： | 1 |
| 16 | Hydrophobic , highly conductive ambient-temperature molten salts 显示文摘 | Dias A P Papageorgiou N | 1996 | Inorg Chem1996，35，5： | 1 |
| 17 | Rapid silver staining and recovery of PCR products separated on polyacrylamide gels polymerase chain reaction product recover显示文摘 | Sanguinetti C J Dias N E Simpson A J | 1994 | Biotechniques1994，17，5： | 1 |
| 18 | Drugs targeting mitochondrial functions to control tumor cell growth显示文摘 | Dias N Bailly C | 2005 | Biochem Pharmacol2005，70，1： | 1 |
| 19 | Massive barite deposits in the convergent margin off Peru:implications for fluid circulation within subduction zones显示文摘 | Aquilina L Dia A N Boulegue J | 1997 | Geochimica et Cosmochimica1997，61，21： | 1 |
| 20 | Hydrophobic, highly conductive ambient-temperature molten sahs显示文摘 | Bonhote P Dias A P Papageorgiou N | 1996 | Inorganic Chemistry1996，35，5： | 1 |
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