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| 1 | Translational pancreatic cancer research:a comparative study on patient-derived xenograft models显示文摘AIM To assess the viability of orthotopic and heterotopic patient-derived pancreatic cancer xenografts implanted into nude mice.METHODS This study presents a prospective experimental analytical follow-up of the development of tumours in mice upon implantation of human pancreatic adenocarcinoma samples. Specimens were obtained surgically from patients with a pathological diagnosis of pancreatic adenocarcinoma. Tumour samples from pancreatic cancer patients were transplanted into nude mice in three different locations(intraperitoneal, subcutaneous and pancreatic). Histological analysis(haematoxylin-eosin and Masson's trichrome staining) and immunohistochemical assessment of apoptosis(TUNEL), proliferation(Ki-67), angiogenesis(CD31) and fibrogenesis(α-SMA) were performed. When a tumour xenograft reached the target size, it was reimplanted in a new nude mouse. Three sequential tumour xenograft generations were generated(F1, F2 and F3).RESULTS The overall tumour engraftment rate was 61.1%. The subcutaneous model was most effective in terms of tissue growth(69.9%), followed by intraperitoneal(57.6%) and pancreatic(55%) models. Tumour development was faster in the subcutaneous model(17.7 ± 2.6 wk) compared with the pancreatic(23.1 ± 2.3 wk) and intraperitoneal(25.0 ± 2.7 wk) models(P = 0.064). There was a progressive increase in the tumour engraftment rate over successive generations for all three models(F1 28.1% vs F2 71.4% vs F3 80.9%, P < 0.001). There were no significant differences in tumour xenograft differentiation and cell proliferation between human samples and the three experimental models among the sequential generations of tumour xenografts. However, a progressive decrease in fibrosis, fibrogenesis, tumour vascularisation and apoptosis was observed in the three experimental models compared with the human samples. All three pancreatic patient-derived xenograft models presented similar histological and immunohistochemical characteristics.CONCLUSION In our experience, the faster development andgreatest number of viable xenografts could make the subcutaneous model the best option for experimentation in pancreatic cancer. | Mercedes Rubio-Manzanares Dorado Luis Miguel Marín Gómez Daniel Aparicio Sánchez Sheila Pereira Arenas Juan Manuel Praena-Fernández Juan Jose Borrero Martín Francisco Farfán López Miguel ángel Gómez Bravo Jordi Muntané Relat Javier Padillo Ruiz | 2018 | World Journal of Gastroenterology2018，24，7： | 2 |
| 2 | Effect of cryopreservation and single layer centrifugation on canine sperm DNA fragmentation assessed by the sperm chromatin dispersion test 显示文摘 | Urbano M Dorado J Ortiz I | 2013 | Anim Reprod Sci2013，143，14： | 1 |
| 3 | Video-nystagmography findings in healthy elderly subjects显示文摘 | Amor Dorado J C Rubio Rodríguez J P Costa Ribas C | 2003 | Acta Otorrinolaringol Esp2003，54，3： | 1 |
| 4 | Image classification with the use of radial basis function neural networks and the minimization of the loealized generalization error显示文摘 | Wing W Y N Dorado A Yeung D S | 2007 | Pattern Recognition2007，40，： | 1 |
| 5 | Influence of the Ion Exchanged Metal (Cu, Co, Ni and Mn) on the Se- lective Catalytic Reduction of NOz Over Mordenite and ZSM-5 显示文摘 | De Lucas A Valverde J L Dorado F | 2005 | Journal of Molecular Catalysis A: Chemical2005，225，1： | 1 |
| 6 | No effect of the CYP1A2* 1F genotype on thioridazine, mesoridazine, sullo ridazine plasma concentrations in psychiatric patients显示文摘 | Dorado P Berecz R Penas LLedo EM | 2007 | Eur J Clin Pharmacol2007，63，5： | 1 |
| 7 | Ultrasound for the diagnosis of female urinary incontinence显示文摘 | Jimenez Cidre MA Lopez Fando Lavalle L Quicios Dorado C | 2006 | Arch Esp Urol2006，59，4： | 1 |
| 8 | Constrained design of polynomial surfaces from geodesic curves显示文摘 | Sánchez-Reyes J Dorado R | 2008 | Computer-Aided Design2008，40，1： | 1 |
| 9 | Effectiveness of a training programme to improve hand hygiene compliance in primary healthcare 显示文摘 | MARTIN - MADRAZO C CANADA - DORADO A SALINERO - FORT MA | 2009 | BMC Public Health2009，9，5： | 1 |
| 10 | Pyrolytic descriptors responsive to changes in humic acid characteristics after long-term sustainable management of dryland farming systems in Central Spain显示文摘 | Dorado J González-Vila F J Zancada M C | 2003 | Journal of analytical and applied pyrolysis2003，68，： | 1 |
| 11 | Crescive entrepreneurship in complex social problems: Institutional conditions for entrepreneurial engagement显示文摘 | Dorado S Ventresca M J | 2013 | Journal of Business Venturing2013，28，1： | 1 |
| 12 | Mechanical throm-bectomy with the Solitaire AB device in large artery occlusions of the anterior circulation:a pilot study显示文摘 | Casta(n)o C Dorado L Guerrero C | 2010 | Stroke2010，41，： | 1 |
| 13 | Development of SCARs by direct sequencing of RAPD products: A practical tool for the introgression and marker-assisted selection of wheat显示文摘 | Hernandez P Martin A Dorado G | 1999 | Molecular Breeding1999，5，： | 1 |
| 14 | Metabolic activity determines efficacy of macroautophagic clearance of pathological oligomeric alpha-synuclein显示文摘 | Yu W H Dorado B Figueroa H Y | 2009 | Am J Pathol2009，175，2： | 1 |
| 15 | TGF-betal induces COX-2 expression and PGE2 synthesis through MAPK and PI3K pathways in human mesangial cells显示文摘 | Rodriguez-Barbero A Dorado F Velasco S | 2006 | Kidney Int2006，70，5： | 1 |
| 16 | Building Sustainable Hybrid Or- ganizations: The Case of Commercial Mierofinanee Organiza- tions显示文摘 | Battilana J Dorado S | 2010 | Academy of Management Journal2010，53，6： | 1 |
| 17 | Mechanical thrombectomy with the Solitaire AB device in huge artery occlusions of the anterior circulation:a pilot study显示文摘 | Castafio C Dorado L Guerrero C | 2010 | Stroke2010，41，8： | 1 |
| 18 | Mechanical thrombectomy with the Solitaire AB device in large artery occlusions of the anterior circulation;a pilot study显示文摘 | Castafto C Dorado L Guerrero C | 2010 | Stroke2010，41，8： | 1 |
| 19 | Transformation of wheat straw in the course of solid-state fermentation by four lignino- lyric basidlomycetes显示文摘 | Dorado J Almendros G Camarero S | 1999 | Enzyme and Microbial Technology1999，25，： | 1 |
| 20 | Anti- psychotic drugs and QTc prolongation: the potential role of CYP2D6 genetic polymorphism显示文摘 | Dorado P Berecz R Pehas-Lled6 EM | 2007 | Expert Opin Drug Metab Toxicol2007，3，1： | 1 |
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