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| 1 | Oral treatment with plecanatide or dolcanatide attenuates visceral hypersensitivity via activation of guanylate cyclase-C in rat models显示文摘AIM To investigate the effects of plecanatide and dolcanatide on maintenance of paracellular permeability, integrity of tight junctions and on suppression of visceral hypersensitivity. METHODS Transport of fluorescein isothiocyanate(FITC)-dextran was measured to assess permeability across cell monolayers and rat colon tissues. Effects of plecanatide and dolcanatide on the integrity of tight junctions in Caco-2 and T84 monolayers and on the expression and localization of occludin and zonula occludens-1(ZO-1) were examined by immunofluorescence microscopy. Anti-nociceptive activity of these agonists was evaluated in trinitrobenzene sulfonic acid(TNBS)-induced inflammatory as well as in non-inflammatory partial restraint stress(PRS) rat models. Statistical significance between the treatment groups in the permeability studies were evaluated using unpaired t-tests.RESULTS Treatment of T84 and Caco-2 monolayers with lipopolysaccharide(LPS) rapidly increased permeability, which was effectively suppressed when monolayers were also treated with plecanatide or dolcanatide. Similarly, when T84 and Caco-2 monolayers were treated with LPS, cell surface localization of tight junction proteins occludin and ZO-1 was severely disrupted. When cell monolayers were treated with LPS in the presence of plecanatide or dolcanatide, occludin and ZO-1 were localized at the cell surface of adjoining cells, similar to that observed for vehicle treated cells. Treatment of cell monolayers with plecanatide or dolcanatide without LPS did not alter permeability, integrity of tight junctions and cell surface localization of either of the tight junction proteins. In rat visceral hypersensitivity models, both agonists suppressed the TNBS-induced increase in abdominal contractions in response to colorectal distension without affecting the colonic wall elasticity, and both agonists also reduced colonic hypersensitivity in the PRS model. CONCLUSION Our results suggest that activation of GC-C signaling might be involved in maintenance of barrier function, possibly through regulating normal localization of tight junction proteins. Consistent with these findings, plecanatide and dolcanatide showed potent antinociceptive activity in rat visceral hypersensitivity models. These results imply that activation of GC-C signaling may be an attractive therapeutic approach to treat functional constipation disorders and inflammatory gastrointestinal conditions. | Illona-Marie Boulete Anusha Thadi Catherine Beaufrand Viren Patwa Apoorva Joshi John A Foss E Priya Eddy Helene Eutamene Vaseem A Palejwala Vassilia Theodorou Kunwar Shailubhai | 2018 | World Journal of Gastroenterology2018，24，17： | 5 |
| 2 | Quantifying cold front induced water transport of a bay with in situ observations using manned and unmanned boats显示文摘The generation of high-resolution data is increasingly important in understanding the complexities of coastal ocean and developing sound management strategies, especially in view of the long-term impact of severe weather systems. The impact of severe weather systems, when integrated over time, can be significant when compared with tidal oscillations. This paper presents a study of water transport out of Vermilion Bay in response to a short,intense event associated with a passing atmospheric cold front, and reports the application of an Acoustic Doppler Current Profiler(ADCP) mounted on an Automated Surface Craft(ASC), known as the auto-boat or unmanned boat, developed in our lab at the Louisiana State University, to generate high resolution data accurately at a fraction of the cost of a manned boat. In our study, we used a manned boat and an unmanned boat, each for over 24 h to cover an entire diurnal tidal cycle, to measure flow velocity profiles to calculate the total transport. A stationary ADCP was deployed in the Southwest Pass of the Vermilion Bay from May 2009 to April2012, providing data almost continuously(with only one major gap), with a 717-day record of water transport between the northern Gulf of Mexico and Vermilion Bay, and demonstrates the importance of the pass in water transport. | WEEKS Eddie ROBINSON Mark E LI Chunyan | 2018 | Acta Oceanologica Sinica2018，37，11： | 2 |
| 3 | Role of MicroRNA 1207-5P and Its Host Gene, the Long Non-Coding RNA Pvtl, as Mediators of Extracellular Matrix Accumulation in the Kidney : Im- plications for Diabetic Nephropathy显示文摘 | Alvarez ML Khosroheidari M Eddy E | 2013 | PLoS One2013，8，77： | 1 |
| 4 | In vitro models to predict blood-brain barrier permeability显示文摘 | Eddy E P Maleet B E Hart T K | 1997 | Adv Drug Del Rev1997，23，123： | 1 |
| 5 | What' s in a Name? A Literature- based Approach to Understanding Mentoring, Coaching, and other Constructs that Describe Developmental Interactions 显示文摘 | D · Abate C P Eddy E R Tannenbaum S I | 2003 | Human Resource Development Review2003，4，2： | 1 |
| 6 | Pfam: A comprehensive database of protein domain families based on seed alignments显示文摘 | Sonnhammer E L Eddy S R Durbin R | 1997 | Proteins1997，28，3： | 1 |
| 7 | Diabetes Risk Calculator: a simple tool for detecting undiagnosed diabetes and pre-diabetes 显示文摘 | HEIKES K E EDDY D M ARONDEKAR B | 2008 | Diabetes Care2008，31，5： | 1 |
| 8 | The Effect of Fluid and Carbohydrate Feedings During Intermittent Cycling Exercise显示文摘 | MURRAY R EDDY D E MURRAY T W | 1987 | Med Sci Sprots Exerc1987，19，6： | 1 |
| 9 | , LDHC: the ultimate testis- specific gene显示文摘 | Goldberg E Eddy EM Duan C | 2010 | J Androl2010，31，1： | 1 |
| 10 | Fibrous sheath of mammalian spermatozoa显示文摘 | Eddy E M Toshimori K O'Brien D A | 2003 | Microsc Res Tech2003，61，1： | 1 |
| 11 | A dynamic programming algorithm for RNA structure prediction including pseudoknots 显示文摘 | Rivas E Eddy SR | 1999 | Journal of Molecular Biology1999，285，5： | 1 |
| 12 | Establishment of critical success factors for construction partnering显示文摘 | Cheng Eddie W L Li Heng love P E D | 2000 | Journal of Management in Engineering2000，16，2： | 1 |
| 13 | Targeted disruption of the estrogen receptor gene in male mice causes alteration of spermat0 genesis and infertility 显示文摘 | EDDY E M WASHBURN T F BUNCH D O | 1996 | Endocrinology1996，137，： | 1 |
| 14 | Recovery and characterization of a minute virus of canines 显示文摘 | Binn L N Lazar E C Eddy G A | 1970 | Infect Immun1970，1，5： | 1 |
| 15 | Phosphorus assimilation in streams and wetland:critical reviews显示文摘 | Eddy K R Kadlec R H Flaig E | 1996 | Environtal Science and Technology1996，31，： | 1 |
| 16 | Sampling criteda for sensor simulation显示文摘 | Eddie L J Timothy C E | 1999 | Opt Eng1999，38，5： | 1 |
| 17 | Effects of nitric oxide synthase inhibi tors and a substrate,L-arginine,on the cardiac function of juvenile salmonid fish显示文摘 | Eddy E B Tibbs P | 2003 | Comparative Biochemistry and Physiology2003，135，2： | 1 |
| 18 | The Pfam protein families database 显示文摘 | Punta M Coggill P C Eberhardt R Y Mistry J Tate J Boursnell C Pang N Forslund K Ceric G Clements J Hcger A Holm L Sonnhammer E L Eddy S R Bateman A Finn R D | 2012 | Nucleic Acids Research2012，40，： | 1 |
| 19 | Nucleotide sequence of yellow fever virus:implications for flavivirus gene expression and evolution 显示文摘 | Rice C M Lenches E M Eddy S R | 1985 | Science1985，229，4715： | 1 |
| 20 | Chemical Information From GC-MS Studies of Ethanol Extract of Andrographis Panicidata and Their Corrosion Inhibition Potentials on Mild Steel in HCL Solution显示文摘 | Eddy N O Awe F E Siaka A A | 2011 | lnt J Electrochem Sci2011，，6： | 1 |
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