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| 1 | Immunohistochemical expression of SP-NK-1R-EGFR pathway and VDR in colonic inflammation and neoplasia显示文摘AIM:To determine the expression of neurokinin-1receptor(NK-1R),phosphorylated epidermal growth factor receptor(p EGFR),cyclooxygenase-2(Cox-2),and vitamin D receptor(VDR)in normal,inflammatory bowel disease(IBD),and colorectal neoplasia tissues from Puerto Ricans.METHODS:Tissues from patients with IBD,colitisassociated colorectal cancer(CAC),sporadic dysplasia,and sporadic colorectal cancer(CRC),as well as normal controls,were identified at several centers in Puerto Rico.Archival formalin-fixed,paraffin-embedded tissues were de-identified and processed by immunohistochemistry for NK-1R,p EGFR,Cox-2,and VDR.Pictures of representative areas of each tissues diagnosis were taken and scored by three observers using a4-point scale that assessed intensity of staining.Tissues with CAC were further analyzed by photographing representative areas of IBD and the different grades of dysplasia,in addition to the areas of cancer,within each tissue.Differences in the average age between the five patient groups were assessed with one-way analysis of variance and Tukey-Kramer multiple comparisons test.The mean scores for normal tissues and tissues with IBD,dysplasia,CRC,and CAC were calculatedand statistically compared using one-way analysis of variance and Dunnett’s multiple comparisons test.Correlations between protein expression patterns were analyzed with the Pearson’s product-moment correlation coefficient.Data are presented as mean±SE.RESULTS:On average,patients with IBD were younger(34.60±5.81)than normal(63.20±6.13,P<0.01),sporadic dysplasia(68.80±4.42,P<0.01),sporadic cancer(74.80±4.91,P<0.001),and CAC(57.50±5.11,P<0.05)patients.NK-1R in cancer tissue(sporadic CRC,1.73±0.34;CAC,1.57±0.53)and sporadic dysplasia(2.00±0.45)were higher than in normal tissues(0.73±0.19).p EGFR was significantly increased in sporadic CRC(1.53±0.43)and CAC(2.25±0.47)when compared to normal tissue(0.07±0.25,P<0.05,P<0.001,respectively).Cox-2 was significantly increased in sporadic colorectal cancer(2.20±0.23 vs 0.80±0.37 for normal tissues,P<0.05).In comparison to normal(2.80±0.13)and CAC(2.50±0.33)tissues,VDR was significantly decreased in sporadic dysplasia(0.00±0.00,P<0.001 vs normal,P<0.001 vs CAC)and sporadic CRC(0.47±0.23,P<0.001 vs normal,P<0.001 vs CAC).VDR levels negatively correlated with NK-1R(r=-0.48)and p EGFR(r=-0.56)in normal,IBD,sporadic dysplasia and sporadic CRC tissue,but not in CAC.CONCLUSION:Immunohistochemical NK-1R and p EGFR positivity with VDR negativity can be used to identify areas of sporadic colorectal neoplasia.VDR immunoreactivity can distinguish CAC from sporadic cancer. | Raymond A Isidro Myrella L Cruz Angel A Isidro Axel Baez Axel Arroyo William A González-Marqués Carmen González-Keelan Esther A Torres Caroline B Appleyard | 2015 | World Journal of Gastroenterology2015，21，6： | 7 |
| 2 | Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease显示文摘AIM: To determine serum vitamin D levels and colonic vitamin D receptor(VDR) expression in inflammatory bowel disease(IBD) and non-IBD patients and correlate these with histopathology.METHODS: Puerto Rican IBD(n = 10) and non-IBD(n = 10) patients ≥ 21 years old scheduled for colonoscopy were recruited. Each patient completed a questionnaire and provided a serum sample and a colonic biopsy of normal-appearing mucosa. For IBD patients, an additional biopsy was collected from visually diseased mucosa. Serum vitamin D levels were measured by ultra-performance liquid chromatography and mass spectrometry. Hematoxylin and eosin stained tissue sections from colonic biopsies were classified histologically as normal or colitis(active/inactive), and scored for the degree of inflammation present(0-3, inactive/absent to severe). Tissue sections from colonic biopsies were also stained by immunohistochemistry for VDR, for which representative diagnostic areas were photographed and scored for staining intensity using a 4-point scale.RESULTS: The IBD cohort was significantly younger(40.40 ± 5.27, P < 0.05) than the non-IBD cohort(56.70 ± 1.64) with a higher prevalence of vitamin D deficiency(40% vs 20%, respectively) and insufficiency(70% vs 50%, respectively). Histologic inflammation was significantly higher in visually diseased mucosa from IBD patients(1.95 ± 0.25) than in normalappearing mucosa from control patients(0.25 ± 0.08, P < 0.01) and from IBD patients(0.65 ± 0.36, P < 0.05) and correlated inversely with VDR expression in visually diseased colonic tissue from IBD patients(r =-0.44, P < 0.05) and from IBD patients with Crohn's disease(r =-0.69, P < 0.05), but not in normal-appearing colonic tissue from control patients or IBD patients. Control and IBD patient serum vitamin D levels correlated positively with VDR expression in normal colon from control and IBD patients(r = 0.38, P < 0.05) and with patient age(r = 0.54, P < 0.01). CONCLUSION: Levels of serum vitamin D correlate positively with colonic VDR expression in visually normal mucosa whereas inflammation correlates negatively with colonic VDR expression in visually diseased mucosa in Puerto Rican patients. | Yamilka Abreu-Delgado Raymond A Isidro Esther A Torres Alexandra González Myrella L Cruz Angel A Isidro Carmen I González-Keelan Priscilla Medero Caroline B Appleyard | 2016 | World Journal of Gastroenterology2016，22，13： | 5 |
| 3 | 快速凝固富钛Ti-V合金的微观组织和储氢性能(英文)显示文摘研究目的在于优化Ti0.8-0.9V0.2-0.1二元合金的相结构成分、微观组织和储氢性能。该合金主要用于从含有大量一氧化碳的高温气态混合物中吸收氢气。Ti0.8-0.9V0.2-0.1合金中的α-(HCP)和β-(BCC)相在纯氢气中基于氢化作用,形成单相FCC结构的氢化物,此过程与合金的化学成分无关。同步辐射X射线衍射的原位分析表明,在含有氢气和10%一氧化碳的混合气体中,只有β相转变成相应的氢化物。快速凝固(RS)处理细化了Ti0.8V0.2和Ti0.9V0.1合金的晶粒组织,而且,快速凝固处理增加了Ti0.9V0.1合金中的β相,其所占比例是普通熔铸条件下的两倍。扫描电子显微镜(SEM)分析表明,Ti0.9V0.1合金含有片状组织,层片的厚度约为300nm。热脱附谱(TDS)显示,微观组织的细化可以加快氢脱附的动力学过程。 | Suwarno SUWARNO Jan Ketil SOLBERG Jan Petter MAEHLEN Bente KROGH Bφrre Tore BφRRESEN Esther OCHOA-FERNANDEZ Erling RYTTER Mario WILLIAMS Roman DENYS Volodymyr A.YARTYS | 2012 | Transactions of Nonferrous Metals Society of China2012，22，8： | 2 |
| 4 | Casein phosphopeptides released by simulated gastrointestinal digestion of infant formulas and their potential role in mineral binding显示文摘 | Esther M Amparo A Reyes B | 2006 | International Dairy Journal2006，16，9： | 1 |
| 5 | Behavior in children with language development disorders显示文摘 | Ulrike W Esther B Gabriele DR | 2003 | Can J Psychiatry2003，48，9： | 1 |
| 6 | The relationship between emotional intelligence,occupational stress and health in nurses:A questionnaire survey 显示文摘 | Jos MAL Esther LZ Pilar B M | 2008 | International Journal of Nursing Studies2008，45，6： | 1 |
| 7 | Development of a strain-specific quantitative method for monitoring Psetutomonas fluorescens EPS62e, a novel biocontrol agent of fire blight显示文摘 | Pujol M Esther B Jordi C | 2005 | FEMS Microbiology Letters2005，249，： | 1 |
| 8 | Casein phosphope tides released by simulated gastrvintestinal digestion of infant formulas andtheir potential role in mineral binding显示文摘 | ESTHER M AMPARO A REYES B | 2006 | International Dairy Journal2006，16，9： | 1 |
| 9 | Assessing cognitive impairment in older people: the Watson clock drawing test 显示文摘 | Esther CA Hagen B Sandilands M | 2004 | Br J Community Nuts2004，9，： | 1 |
| 10 | New evidence against hydroxyl radicals as reactive intermediates in the thermal and photochemically enhanced fenton reactions显示文摘 | STEFAN H B ESTHER O SILVIA S | 1998 | J Phys Chem A1998，102，8： | 1 |
| 11 | Drotrecogin alfa(activated)for the treatment of meninggococcal purpura fulminans 显示文摘 | Esther B Stephan B Roland I | 2003 | Crit Care Med2003，29，： | 1 |
| 12 | A Comprehensive Centralized Approach for Voltage Constraints Management in Active Distribution Grid显示文摘 | Florin Capitanescu Ilya Bìlibin Esther Romero Ramos | 2014 | IEEE TRANSACTIONS ON POWER SYSTEMS2014，29，2： | 1 |
| 13 | Decomposition of transgenic Bacillus thuringiensis maize by microorganisms and woodlice Porcellio scaber (Crustacea: Isopoda)显示文摘 | Esther N Kach B Nentwig W | 2000 | Basic Applied Ecology2000，1，： | 1 |
| 14 | Engineered autolytic yeast strains secreting Kluyveromyces lactis β-galactosidase for production of heterologous proteins in lactose media显示文摘 | MANUEL B ESTHER R B ESPERANZA C M | 2004 | Journal of Biotechnology2004，109，： | 1 |
| 15 | Combined oral benzo pyrene and inhalatory ozone exposure have no effect on lung tumor development in DNA repair-deficient Xpa mice显示文摘 | ESTHER M H ANNEMIEKE D V RUDOLF B B | 2003 | Carcinogenesis2003，24，3： | 1 |
| 16 | The challenge of micropollutants in aquatic systems显示文摘 | Schwarzenbach R P Esther B I Escher K | 2006 | Science2006，313，5790： | 1 |
| 17 | A possible role for secreted ferritin in tissue iron distribution显示文摘 | ESTHER G MEYRON H SHIRLY M B | 2011 | J Neural Transm2011，118，： | 1 |
| 18 | Molecular effectors of muhiple cell death pathways initiated by photodynamic therapy 显示文摘 | Esther B Michael D Patrizia A | 2007 | Biochimi Biophys Acta2007，1776，1： | 1 |
| 19 | Patient-doctorinteraction,psychobehavioural characteristics and mentaldisorders in patients with suspected allergies: do they predict'medically unexplained symptoms'?显示文摘 | Constanze H W’Martine G Esther B | 2011 | Acta dermato-venereologica}2011，91，： | 1 |
| 20 | A redundancy elimination approach towards summary refinement 显示文摘 | ESTHER H M SASWATI M SAKTHI B | 2014 | International Conference on Future Information Engineering2014，10，： | 1 |
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