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| 1 | 二氢嘧啶脱氢酶、胸苷酸合成酶、c-myc和p53基因在小鼠肝组织中表达的昼夜节律性研究(英文)显示文摘背景与目的:5-Fluorouracil(5-FU)主要通过抑制胸苷酸合成酶发挥抗癌作用,而二氢嘧啶脱氢酶是5-FU体内代谢的一个起始酶和限速酶。在本研究中,我们对二氢嘧啶脱氢酶、胸苷酸合成酶、c-myc和p53基因在小鼠肝脏中表达的昼夜节律进行研究。方法:本实验共采用了24只雄性B6D2F1小鼠。所有实验动物在实验中进行了4周光照同步化(12h光照:12h黑暗)。体温和休息-活动节律通过腹腔埋置的微型感受器进行动态监测。动物分别在3,7,11,15,19,23HALO(灯亮后小时数)被处死并取出肝脏冻存。肝脏标本中抽提的总RNA用LightCycler-SYBRGreenITM扩增系统进行实时定量RT-PCR。结果:小鼠的体温和休息-活动均呈现明显和相似的昼夜节律,其峰值均位于黑暗期(相当于小鼠活动相),谷值则位于光照期(相当于小鼠休息相)。二氢嘧啶脱氢酶在mRNA水平呈现出明显的昼夜节律性,其峰值时间位于16HALO左右即位于小鼠活动相(P=0.0012)。胸苷酸合成酶在mRNA水平具有昼夜节律表达的趋势,其峰值时间出现在光照期(P=0.079)。C-myc和p53在mRNA水平的节律性表达在本实验中无统计学意义。结论:二氢嘧啶脱氢酶和胸苷酸合成酶在mRNA水平呈现出昼夜节律性,二者峰值时间相差约12h,这一结果提示可以利用二者在转录水平的时间差异用5-FU进行时间化疗。 | 吴明玮 冼励坚 李晓梅 lnnominato Pasquale lvi Francis | 2004 | 癌症2004，23，3： | 22 |
| 2 | 移植于裸鼠的人鼻咽癌细胞DNA合成生物节律的初步研究显示文摘目的:探索鼻咽癌细胞DNA合成的生物节律为临床制订鼻咽癌时辰化疗方案提供必要的参数。方法:BALB/C裸小鼠15只,置于程控的独立光照系统中(12h光照,12h黑暗)中同步化至少3周。然后双侧腋下接种人鼻咽低分化鳞癌细胞裸小鼠移植瘤CNE2,成瘤后按灯亮后3、9、15、21h(即3、9、15、21HALO)的时间点取瘤块,制成单个细胞悬液固定,染色后流式细胞仪测DNA含量。用SPSS软件系统ANOVA法检验各期细胞在4个时间点差异的显著性,用Cosinor法考察G1、S、G2/M期细胞在24h的分布是否符合余弦函数。结果:G1、S、G2/M期细胞在3、9、15、21HALO的分布随时间变化有显著性差异,G1、G2/M期细胞变化符合余弦节律。结论:移植于裸鼠的人鼻咽癌细胞的DNA合成随昼夜交替呈节律性变化。 | 孙健 冼励坚 曹弃元 叶燕丽 LIU Xu-hui LI Xiao-mei Francis Lé vi | 2001 | 癌症2001，20，2： | 21 |
| 3 | 肿瘤的时辰治疗显示文摘在实验动物中,30多种抗癌药物的细胞毒作用24小时中随给药时间不同而变化超过50%.其中的机制涉及细胞代谢,增殖过程以及药物的药动学的时间节律变化而且,在实验肿瘤模型中在最小毒性时间进行化疗通常可以达到最大的抗瘤效果. | Francis Lévi 孙健 冼励坚 | 2001 | 广州医药2001，32，2： | 6 |
| 4 | Diagnosis and Epidemiology of H elicobacter pylori Infection显示文摘 | Xavier Calvet María‐José Ramírez Lázaro Philippe Lehours Francis Mégraud | 2013 | Helicobacter2013，，： | 5 |
| 5 | Inflammatory bowel disease in Asia: A systematic review显示文摘 | Lani Prideaux Michael A Kamm Peter P De Cruz Francis K L Chan Siew C Ng | 2012 | Journal of Gastroenterology and Hepatology2012，，8： | 5 |
| 6 | Auditor brand name reputations and industry specializations显示文摘 | Allen T Craswell Jere R Francis Stephen L Taylor | 1995 | Journal of Accounting and Economics1995，，3： | 4 |
| 7 | Regulator of myeloid differentiation and function:The secret life of Ikaros显示文摘Ikaros (also known as Lyf-1) was initially described as a lymphoid-specific transcription factor.Although Ikaros has been shown to regulate hematopoietic stem cell renewal,as well as the development and function of cells from multiple hematopoietic lineages,including the myeloid lineage,Ikaros has primarily been studied in context of lymphoid development and malignancy.This review focuses on the role of Ikaros in myeloid cells.We address the importance of post-transcriptional regulation of Ikaros function;the emerging role of Ikaros in myeloid malignancy;Ikaros as a regulator of myeloid differentiation and function;and the selective expression of Ikaros isoform-x in cells with myeloid potential.We highlight the challenges of dissecting Ikaros function in lineage commitment decisions among lymphoidmyeloid progenitors that have emerged as a major myeloid differentiation pathway in recent studies,which leads to reconstruction of the traditional map of murine and human hematopoiesis. | Olivia L Francis Jonathon L Payne Kimberly J Payne | 2011 | World Journal of Biological Chemistry2011，2，6： | 4 |
| 8 | 在 Chinathe 的机关移植第 21 世纪显示文摘 <正>Introduction China has emerged as an economic power internationally and it now naturally seeks to influence the conduct of medical affairsto improve the condition of the Chinese people; | Francis L Delmonico | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，4： | 2 |
| 9 | 在 7T 的多原子的磁性的回声光谱学的应用显示文摘 AIM: To discuss the advantages of ultra-high field (7T) for 1H and 13C magnetic resonance spectroscopy (MRS) studies of metabolism.made at both 3 and 7T using 1H MRS. Measurements of glycogen and lipids in muscle were measured using 13C and 1H MRS respectively. RESULTS: In the brain, increased signal-to-noise ratio (SNR) and dispersion allows spectral separation of the amino-acids glutamate, glutamine and γ-aminobutyric acid (GABA), without the need for sophisticated editing sequences. Improved quantification of these me-tabolites is demonstrated at 7T relative to 3T. SNR was 36% higher, and measurement repeatability (% coefficients of variation) was 4%, 10% and 10% at 7T, vs 8%, 29% and 21% at 3T for glutamate, glutamine and GABA respectively. Measurements at 7T were used to compare metabolite levels in the anterior cingulate cortex (ACC) and insula. Creatine and glutamate levels were found to be significantly higher in the insula compared to the ACC (P < 0.05). In muscle, the increased SNR and spectral resolution at 7T enables interleaved studies of glycogen (13C) and intra-myocellular lipid (IMCL) and extra-myocellular lipid (EMCL) (1H) following exercise and refeeding. Glycogen levels were sig-nificantly decreased following exercise (-28% at 50% VO2 max; -58% at 75% VO2 max). Interestingly, levels of glycogen in the hamstrings followed those in the quadriceps, despite reduce exercise loading. No changes in IMCL and EMCL were found in the study. CONCLUSION: The demonstrated improvements in brain and muscle MRS measurements at 7T will increase the potential for use in investigating human metabolism and changes due to pathologies. | Mary C Stephenson Frances Gunner Antonio Napolitano Paul L Greenhaff Ian A MacDonald Nadeem Saeed William Vennart Susan T Francis Peter G Morris | 2011 | World Journal of Radiology2011，3，4： | 2 |
| 10 | Tumor Marker Evolution: Comparison with Imaging for Assessment of Response to Chemotherapy in Patients with Colorectal Liver Metastases显示文摘 | Robbert J. Haas Dennis A. Wicherts Eduardo Flores Michel Ducreux Francis Lévi Bernard Paule Daniel Azoulay Denis Castaing Antoinette Lemoine René Adam | 2010 | Annals of Surgical Oncology2010，，4： | 2 |
| 11 | Community-acquired Meticillin-resistant Staphylococcus Aureus: an Emerging Threat显示文摘 | Zetola N Francis J S Nuermberger E L | 2005 | Lancet Infect Dis2005，5，5： | 1 |
| 12 | Insulin-like growth factors in poultry 显示文摘 | MCMURTYR J P FRANCIS G L UPTON Z | 1997 | Domest Anim Endocrinol1997，14，4： | 1 |
| 13 | Formation of thaumasite in synthetic cement mineral slurries显示文摘 | Purnell P Francis O J Page C L | 2003 | Cem Concr Compos2003，25，： | 1 |
| 14 | Relationship quality and burden among caregivers for latestage cancer 显示文摘 | Francis L E Worthington J Kypriotakis G | 2009 | Support Care Cancer2009，18，11： | 1 |
| 15 | Assessment of erosion haz- ard with the USLE and GIS A ease study of the Upper Ewaso Nglro North basin of Kenya显示文摘 | Bancy M Mati Royston P C Morgan Francis N Gichuki John N Quinton Tim R Brewer Hans P l iniger | 2000 | lnternation Journal of Applied Earth Ob- servation and Geoinformation2000，2，2： | 1 |
| 16 | Fault diagnosis based on rough set theory显示文摘 | FRANCIS E H SHEN L X | 2003 | Engineering applications of artificial intelligence2003，16，1： | 1 |
| 17 | Support vector machine with adaptive parameters in financial time series forecasting显示文摘 | Cao L J Tay Francis E H | 2003 | IEEE Trans- actions on Neural Networks2003，6，14： | 1 |
| 18 | Evidence for Abstract, Schematic Knowledge of Three Spatial Diagram Representations 显示文摘 | Novick L R Hurley S M Francis M | 1999 | Memory Cognition1999，27，2： | 1 |
| 19 | Solubilization of cyclosporin A in dextran-g-polyethyleneglycolalkyl ether polymeric micelles显示文摘 | Francis M F Lavoie L Winnik F M | 2003 | Eur J Pharm Biopharm2003，56，3： | 1 |
| 20 | The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco BY-2 cells 显示文摘 | de Pinto M C Francis D de Gara L | 1999 | Protoplasma1999，209，12： | 1 |
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