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| 1 | Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection显示文摘AIM To demonstrate the relationship betweenH-ras oncogene and hepatocellular carcinoma(HCC) metastasis.METHODS Activated H-ras oncogene wastransfected into SMMC 7721, a cell line derivedfrom human HCC, by calcium phosphatetransfection method. Some metastasis-relatedparameters were detected in vitro, includingadhesion assay, migration assay, expression ofcollagenase ⅣV (c ⅣV ase) and epidermal growthfactor receptor (EGFR).RESULTS The abilities of H-ras-transfected cellclones in adhesion to laminin (LN) or fibronectin(FN), migration, c Ⅳ ase secretion increasedmarkedly, and the expression of EGFR elevatedmoderately. More importantly, these alterationswere consistent positively with the expressionof p21, the protein product of H-ras oncogene.CONCLUSION H-ras oncogene could inducethe metastatic phenotype of HCC cell in vitro toraise its metastatic potential. | Qing Wang~1 Zhi Ying Lin~2 Xiao Li Feng~3 ~1Department of Microbiology,Medical Center of Fudan University.the former Shanghai Medical University,Shanghai 200032,China ~2Liver Cancer Institute,Zhongshan Hospital,Shanghai 200032,China ~3Shanghai Institute of Biochemistry,Academy Sinica,Shanghai 200031,ChinaQing Wang earned master degree from Shanghai Medical University in 1996,now a senior lecturer of microbiology,specialized in the role of oncogcncs on tumor metastasis,having 8 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 48 |
| 2 | Influence of interannual variability of Antarctic oscillation on mei-yu along the Yangtze and Huaihe River valley and its importance to prediction显示文摘Both correlation analysis and case study indi-cate that Antarctic oscillation (AAO) is closely related withsummer rainfall in eastern China. When AAO is stronger inboreal spring, especially in May, there is more mei-yu rain-fall in summer with a longer period along the Yangtze andHuaihe River valley. In contrast, there is less rainfall with ashorter period corresponding to a weaker AAO. Besides, ananomalous AAO changes the position and intensity of severalcirculation systems, which are important to summer rainfallalong the Yangtze and Huaihe River valley. Furthermore, theAntarctic sea ice is negatively correlated with the intensity ofAAO with a 6-month leading time. The result in this studyprovides a new method for the prediction of mei-yu. | GAO Hui~(1,2), XUE Feng~1 WANG Huijun~1 1. Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China 2. Department of Atmospheric Sciences, Nanjing Institute of Meteorology, Nanjing 210044. China | 2003 | Chinese Science Bulletin2003，48，S2： | 34 |
| 3 | Fuzzy c-means clustering with non local spatial information for noisy image segmentation显示文摘 | Feng Zhao (1) add_zf1119@hotmail.com Licheng Jiao (1) Hanqiang Liu (1) | 2011 | Frontiers of Computer Science2011，5，1： | 31 |
| 4 | PHYSICAL MODELING FOR THE MECHANISMS OF BEAD FORMATION DEFECTS BASED ON THE STABILITY OF LIQUID METAL显示文摘Based on the theory of stability of the liquid metal under the action of surface tension,a physical mod- el is established for the mechanisms of the bead formation deferct’ humping’, which is generated during high - speed welding.A boundary function is introduced to correct the model,theoretical results got by the model correspond well with the experimental phenomena. A two - dimensional conducting model is adoopted in the simulation of the temperature field during high - speed welding.Finally, the factors acting upon the stability of liquid metal are analyzed. | S. Y. Yin L. Feng J. Z. Ding and S. J. Chan( 1) Beijing Polytechnic University, Beijing 100022, China 2) National Key Laboratory of Advanced Wdlding Production Technology, HIT, Harbin 150001, China) | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，1： | 28 |
| 5 | Hepatitis C virus infection of human hepatoma cell line 7721 in vitro显示文摘AIM To establish a cell culture system with long-term replication of hepatitis C virus in vitro.``METHODS Human hepatoma cell line 7721 was tested for its susceptibility to HCV by incubating with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various phases during the culturing periods The presence of HCV RNA, the expression of HCV antigens in cells and/or supernatant were examined by RT-PCR, in situ hybridization and immunohistochemistry respectively.``RESULTS The intracellular HCV RNA was first detected on d 2 after infection and then could be intermittently detected in both cells and supernatant over a period of at least three months. The expression of HCV NS3, CP10antigens could be observed in cells. The fresh cells could be infected by supematant from cultured infected cells and the transmission of viral genome from HCV-infected 7721 cells to PBMCs was also observed.``CONCLUSION The hepatoma line 7721 is not only susceptible to HCV but also supports its long-term replication in vitro. | Zhi-Qiang Song~1 Fei Hao~1 Feng Min~2 Qiao-Yu Ma~2 Guo-Dong Liu~2 Department of Dermatology~1Department of Infectious Diseases~2,Southwest Hospital,Third Military Medical University,Chongqing 400038,China | 2001 | World Journal of Gastroenterology2001，7，5： | 26 |
| 6 | COMPUTERIZED SIMULATION AND EXPERIMENTAL RESEARCH ON SOFT-SWITCHING ARC WELDING INVERTER POWER SOURCE显示文摘Based on the existing component models in the Pspice software package, a combined model for Insulat- ed the Bipolar Transistor (IGBT) is established, in which a non - linear is introduced to represent the parasitic capacitance. Using this model, computerized simulation is conducted for the FB - ZVZCS - PWM soft - ewitching converter,the switching and energy-transferring characteristics of the components are analyzed.The simulation results are testified by experiments.It is proved that by abopting appropriate models,computerized simulation becomes an effective tool for investigation of arc welding inverter power source. | S. J. Chen S. Y. Yin L. Feng and L. Y. Li( 1) School of Materials Science and Engineering, Beijing Polytechnic University, Beijing 100022, China 2)National Key Laboratory of Advanced Welding Production Technology, HIT, Harbin 150001, China) | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，1： | 24 |
| 7 | Lightning activity and precipitation structure of hailstorms显示文摘By using the cloud-to-ground (CG) lightning location data from the lightning detection network of He- nan Province, surface Doppler radar data and standard orbit data of PR, TMI and LIS on TRMM satellite, the spatial and temporal characteristics of CG lightning flashes in 10 severe hailstorms are analyzed. The results show that the percentage of +CG lightning in these hailstorms is high with an average value of 45.5%. There is a distinct increase in CG flash rate during the rapid development stage of hailstorms. The hailstone falling corresponds to an active positive flash period, and the increase of +CG flash rate is generally accompanied with a decrease of –CG flash rate. The flash rate declines rapidly during the dissipating stage of hailstorms. The precipitation structure and lightning activity in two typical hail- storms are studied in detail. It is found that strong convective cells with reflectivity greater than 30dBZ mainly are situated in the front region of hailstorms, whereas the trailing stratiform region is in the rear part of the hailstorms. The maximum heights of echo top are higher than 14 km. Convective rain con- tributes much more rainfall to the total than stratiform rain, and the convective rain takes about 85% and 97% of the total in the two cases, respectively. Total lightning in the hailstorms is very active with the flash rate up to 183 fl/min and 55 fl/min, respectively. The results also indicate that most lightning flashes occurred in the echo region greater than 30 dBZ and its immediate periphery. The probability of lightning occurrence is 20 times higher in the convective region than in the stratiform region. The result suggests that the lightning information is helpful to the identification of convective rain region. The linear relationship between flash rate and ice water content is disclosed primarily. | FENG GuiLi1,2, QIE XiuShu3,1, YUAN Tie1 & NIU ShuZhen4 1 Laboratory of Lightning and Thunderstorm, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China 2 Shandong Research Institute of Meteorology, Jinan 250031, China 3 Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China 4 The Meteorological Observatory of Henan Province, Zhengzhou 450003, China | 2007 | Science China Earth Sciences2007，50，4： | 22 |
| 8 | Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer显示文摘Omega-3(ω-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of ω-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene. | PAN DengKe1, ZHANG Li1, ZHOU YanRong2, FENG Chong1, LONG Chuan1, LIU Xiao1, WAN Rong3, ZHANG Jian3, LIN AiXing3, DONG EnQiu4, WANG ShuChen4, XU HouGang4 & CHEN HongXing2 1 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2 Beijing Institute of Biotechnology, Beijing 100071, China 3 China Agricultural University, Beijing 100193, China 4 Yutian Breeding Swine Farms, Yutian 064104, China | 2010 | Science China(Life Sciences)2010，53，4： | 21 |
| 9 | Efects of a bioartificial liver support system on a cetaminophen-induced acute liver failure canines显示文摘Seeinvitedcommentaryonpage286Subjectheadingsliversupportsystem;acuteliverfailure;canines;porcinehepatocytes;bioartificiallive... | XUE Yi Long 1, ZHAO Shi Feng 2, ZHANG Zuo Yun 1, WANG Yue Feng 1, LI Xin Jian 1, HUANG Xiao Qiang 3, LUO Yun 1, HUANG Ying Cai 4 and LIU Cheng Gui 1 | 1999 | World Journal of Gastroenterology1999，5，4： | 19 |
| 10 | Gene therapy for human colorectal carcinoma using human CEA promoter controlled bacterial ADP-ribosylating toxin genes:PEA and DTA gene transfer显示文摘INTRODUCTIONAmajorrequirementforinvivogenetherapyforcanceristotargetthetherapeuticgeneexpresiontomalignanttissuesbyselectingp... | CAO Guang Wen 1, QI Zhong Tian 1, PAN Xin 1, ZHANG Xiao Qin 1, MIAO Xiao Hui 1, FENG Yan 1, LU Xin Hua 1, Shigeki Kuriyama 2 and DU Ping 1 | 1998 | World Journal of Gastroenterology1998，4，5： | 18 |
| 11 | Caveolin-1 gene silencing promotes the activation of PI3K/AKT dependent on Erα36 and the transformation of MCF10A^(CE)显示文摘ERα36,a variant of estrogen receptor-α,acts as a dominant-negative factor in both estrogen-dependent and estrogen-independent transactivation signaling pathways,and is a key factor in the promotion,progression and prognosis of breast cancers.Caveolin-1,a 22-to 24-kD integral membrane protein,may function as a tumor suppressor in inhibiting of many growth-promoting signaling pathways.It was shown that downregulation of Caveolin-1 strengthens the interaction of ERα and Caveolin-1.In conclusion,Caveolin-1 gene silencing activated the PI3K/AKT signaling pathway in an ERα36-dependent way.Our finding may provide a promising therapeutic target of breast cancer. | FENG Shuang1,WANG Yang1,WANG Xi1,WANG ZhaoYi3,CUI YuYing1,LIU Jing4,ZHAO ChunHui1,JIN Mei1,2 & ZOU Wei1,2 1 College of Life Science,Liaoning Normal University,Dalian 116029,China 2 Liaoning Key Laboratory of Biotechnology and Molecular Drug R&D,Dalian 116029,China 3 Cancer Center,Creighton University,2500 California Plaza,Omaha,NE 68178,USA 4 First Clinical Hospital of Dalian Medical University,Dalian 116011,China | 2010 | Science China(Life Sciences)2010，53，5： | 16 |
| 12 | Sequencing of PCR amplified HBV DNA pre-c and c regions in 2.2.15 cells and antiviral action by targeted antisense oligonucleotide directed against sequence显示文摘INTRODUCTIONManystudieshaveshownthatantisenseoligonucleotides(ASONs)canefficientlyinhibitHBVDNAreplicationandexpresioninvitro... | ZHOUG Sen 1, WEN Shou Ming 2, ZHANG Ding Feng 3, WANG Quan Li 4, WANG Seng Qi 4 and REN Hong 3 | 1998 | World Journal of Gastroenterology1998，4，5： | 16 |
| 13 | Clinical significance of Fas antigen expression in gastric carcinoma显示文摘INTRODUCTIONInordertodeterminewhetherFasantigenplaysaroleinthegastriccarcinogenicsequence,animmunohistochemicalstudyofFasant... | LIU Hai Feng 1, LIU Wei Wen 1, FANG Dian Chun 1, LIU Feng Xian 2 and HE Guang You 2 | 1999 | World Journal of Gastroenterology1999，5，1： | 15 |
| 14 | Immunohistochemical detection of proliferating cell nuclear antigen in hepatocellular carcinoma显示文摘ImmunohistochemicaldetectionofproliferatingcelnuclearantigeninhepatocelularcarcinomaWANGDong1,SHIJingQuan2andLIUFengXuan3Su... | WANG Dong 1, SHI Jing Quan 2 and LIU Feng Xuan 3 | 1997 | World Journal of Gastroenterology1997，3，2： | 15 |
| 15 | Sea level variations in the South China Sea inferred from satellite gravity,altimetry,and oceanographic data显示文摘Sea level variations(SLVs) can be divided into two major components:the steric SLV and the mass-induced SLV.These two components of SLV in the South China Sea(SCS) are studied by using satellite altimetry,GRACE(Gravity Recovery and Climate Experiment) satellite gravity,and oceanographic data on annual and inter-annual timescales.On the annual timescale,the geographic distribution of mass-induced SLV's amplitude jointly estimated from altimetry and the ECCO(Estimation of the Circulation and Climate of the Ocean) model agrees very well with that from GRACE.GRACE observes obvious seasonal mass-induced SLV in the SCS with annual amplitude of 2.7±0.4 cm,which is consistent with the annual amplitude of 2.7±0.3 cm estimated from the steric-corrected altimetry.On the inter-annual timescales,the mean SLV in the SCS shows a large oscillation,which is mainly caused by the steric effect.The trend of mean SLV inferred from altimetry in the SCS is 5.5±0.7 mm/yr for the period of 1993-2009,which is significantly higher than the global sea level rise rate of 3.3±0.4 mm/yr in the same period.There is no obvious trend signal in the mass-induced SLV detected from GRACE that indicates the water exchange between the SCS and its adjacent seas and land is in balance within the study period. | FENG Wei 1,2,ZHONG Min 1,3 & XU HouZe 1,3 1 State Key Laboratory of Geodesy and Earth’s Dynamics,Institute of Geodesy and Geophysics,Chinese Academy of Sciences,Wuhan 430077,China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China 3 Institute of Geophysics,Huazhong University of Science and Technology,Wuhan 430074,China | 2012 | Science China Earth Sciences2012，55，10： | 15 |
| 16 | In situ experiments on width and evolution characteristics of excavation damaged zone in deeply buried tunnels显示文摘The seven long tunnels of Jinping II hydropower station are deeply buried.The width and evolution characteristics of excavation damaged zone(EDZ) are the key problem to the design of tunnels excavation and supports.In order to study this problem,several specific experimental tunnels with different overburden and geometric sizes were excavated at this site.Digital borehole camera,sliding micrometer,cross-hole acoustic wave equipment and acoustic emission apparatus were adopted.This paper introduced the comprehensive in situ experimental methods through pre-installed facilities and pre-drilled boreholes.Typical properties of the surrounding rock mass,including cracks,deformation,elastic wave and micro fractures,were measured during the whole process of the tunnel excavation.The width and characteristics of formation and evolution of tunnels EDZ were analyzed under different construction methods involving of TBM and drilling and blasting,the test tunnels were excavated by full-face or two benches.The relationships between EDZ and tunnel geometry sizes,overburden and excavation method were described as well.The results will not only contribute a great deal to the analysis of rock mass behavior in deeply buried rock mass,but also provide direct data for support design and rockburst prediction. | LI ShaoJun 1,FENG XiaTing 1,LI ZhanHai 3,CHEN BingRui 1,JIANG Quan 1,WU ShiYong 2,HU Bin 2 &XU JinSong 2 1 State Key Laboratory of Geomechanics and Geotechinical Engineering,Institute of Rock and Soil Mechanics,Chinese Academy of Sciences,Wuhan 430071,China 2 Ertan Hydropower Development Company,Ltd.,Chengdu 610051,China 3 School of Resources and Civil Engineering,Northeastern University,Shenyang 110004,China | 2011 | Science China(Technological Sciences)2011，54，S1： | 13 |
| 17 | Study on Quenching and Tempering Process of Q960 High Strength Steel for Construction Machinery显示文摘To develop the Q960 high-strength quenched and tempered steel plates for construction machinery,the effects of quenching and tempering treating regime on the microstructures and mechanical properties were investigated.The results show that the perfect austenization and fine grain size can be achieved by the optimum quenching process that is quenching temperature 900℃ and holding time 20min.Considering performance and production efficiency,the optimum tempering process parameters are found that tempering temperature 600℃ and holding time 40min.The excellent overall properties of specimens with tempered sorbite microstructure can be ultimately obtained.The yield strength is 1030MPa,tensile strength 1080MPa,percentage elongation 16.8% and the Charpy impact energy 144J at-40℃.All these indexes come up to the National Standard GB/T 16270-2009. | WANG Zhao-dong 1,KANG Jian 1,LU Feng 1,2,DENG Xiang-tao 1,WANG Chao 1,WANG Guo-dong 1 (1.State Key Lab of Rolling and Automation,Northeastern University,Shenyang 110004,Liaoning,China 2.Wide Heavy Plate Project Department of Tangshan Iron and Steel Corporation,Tangshan 063000,Hebei,China) | 2011 | Journal of Iron and Steel Research(International)2011，18，S1： | 13 |
| 18 | A decision-making model of development intensity based on similarity relationship between land attributes intervened by urban design显示文摘The paper presents a dynamic model intervened by urban design for the decision-making of land development intensity, which expresses the inherent interaction mechanism between lands based on the evaluation of land attributes and their similarity relationship. Each land unit is described with several factors according to their condition and potential for development, such as land function, accessibility, historical site control, landscape control, and so on. Then, the dynamic reference relationship between land units is established according to the similarity relationship between their factors. That means lands with similar conditions tend to have similar development intensities, which expresses the rule of the spontaneous urban development. Therefore, the development intensities of the pending lands can be calculated by the confirmed ones. Furthermore, the system can be actively intervened by adjusting the parameters according to urban design or planning intentions. And the reaction of the system offers effective support and reference for reasonable decision. The system with multiple intervention input is not only a credible tool for deriving development intensities, but also a platform to activate urban design conception. Above all, the system as a socio-technical tool integrates the optimization of form, function and environment, and embodies the principle of impersonality, justice and flexibility in the decision of land development intensity. | WANG JianGuo1, ZHANG Yu2 & FENG Han3 1 School of Architecture, Southeast University Key Laboratory for Urban and Architectural Heritage Conservation, Ministry of Education, Nanjing 210096, China 2 School of Architecture, Southeast University, Nanjing 210096, China 3 Faculty of Architecture, Delft University of Technology, 2628 CR Delft, Netherlands | 2010 | Science China(Technological Sciences)2010，53，7： | 12 |
| 19 | Wide area real time kinematic decimetre positioning with multiple carrier GNSS signals显示文摘This paper presents the technical basis for wide area real time decimetre positioning services using multiple carrier signals transmitted by future GNSS such as modernized GPS and Compass systems. The first step is to form two ionosphere-reduced extra-widelanes (EWL) that have the minimal total noise levels in cycles, considering the effects of the ionospheric and tropospheric delays, orbital error, and phase noise terms in various observational environments. The proposed three carrier ambiguity resolution approach can determine the integer ambiguities of the selected EWL signals with geometry-free and geometry- based estimations respectively over the distances of typically hundreds of kilometres. With two ambiguity-fixed EWL signals, the kinematic positioning solutions can be achieved in decimetre level accuracy. A semi-simulation method is employed to generate three frequency Compass data to demonstrate the above expected performance for decimetre positioning services. Both theoretical and experimental results have demonstrated the overall 3D root mean square accuracy of better than 15 cm obtained through with a phase smoothing process over 120 epochs. The dominating error factor in this positioning result is the residual tropospheric biases, which would become less correlated as the baselines grow beyond hundreds of kilometres. With respect to dual-frequency based precise point positioning and wide area differential positioning solutions, a major advantage of using the third frequency signals is the convergence time being shorten from tens of minutes to a few minutes. In addition, due to the instant ambiguity resolution capability, phase breaks or cycle slips no longer affect the continuity of the solutions. This reliable decimetre positioning capability introduces a significant improvement to safety-of-life, liability-critical and professional applications. | FENG YanMing1 & LI BoFeng1,2 1 Faculty of Science and Technology, Queensland University of Technology, GPO Box 2434, QLD 4001, Australia 2 Department of Surveying and Geo-informatics Engineering, Tongji University, Shanghai 200092, China | 2010 | Science China Earth Sciences2010，53，5： | 11 |
| 20 | Arsenic trioxide promotes mitochondrial DNA mutation and cell apoptosis in primary APL cells and NB4 cell lines显示文摘This study aimed to investigate the effects of arsenic trioxide(As2O3) on the mitochondrial DNA(mtDNA) of acute promyelocytic leukemia(APL) cells.The NB4 cell line was treated with 2.0 μmol/L As2O3 in vitro,and the primary APL cells were treated with 2.0 μmol/L As2O3 in vitro and 0.16 mg kg-1 d-1 As2O3 in vivo.The mitochondrial DNA of all the cells above was amplified by PCR,directly sequenced and analyzed by Sequence Navigatore and Factura software.The apoptosis rates were assayed by flow cytometry.Mitochondrial DNA mutation in the D-loop region was found in NB4 and APL cells before As2O3 use,but the mutation spots were remarkably increased after As2O3 treatment,which was positively correlated to the rates of cellular apoptosis,the correlation coefficient:rNB4-As2O3=0.973818,and rAPL-As2O3=0.934703.The mutation types include transition,transversion,codon insertion or deletion,and the mutation spots in all samples were not constant and regular.It is revealed that As2O3 aggravates mtDNA mutation in the D-loop region of acute promyelocytic leukemia cells both in vitro and in vivo.Mitochondrial DNA might be one of the targets of As2O3 in APL treatment. | MENG Ran1,ZHOU Jin2,SUI Meng3,LI ZhiYong4,FENG GuoSheng1 & YANG BaoFeng2 1 Division of Neurology,the Ninth Clinical Medical College of Beijing University(Shijitan Hospital),Beijing 100038,China 2 The First Hospital of Harbin Medical University,Harbin 150001,China 3 Management Information System,University of International Business and Economics,Beijing 100029,China 4 Department of Radiology,Cambridge University Hospitals NHS Foundation Trust Box 219 Level 5,Addenbrooke’s Hospital Cambridge CB2 0QQ,UK | 2010 | Science China(Life Sciences)2010，53，1： | 10 |
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