|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | The suffulta mutation in tomato reveals a novel method of plastid replication during fruit ripening显示文摘 | FORTH D PYKE K A | 2006 | Journal of Experimental Botany2006，57，9： | 1 |
| 3 | Titanium dioxide produced by chloride process (third) 显示文摘 | Gas-phase Oxidation Reseach Team of the Forth Reseach Office in Paint & Coatings Industry Division of Gansu Painting Plant | 1974 | Paint & Coatings Industry1974，，3： | 1 |
| 4 | Effect of acute and subchronic exposure to cadmium on the retention of iron in rats显示文摘 | Schafer S G Forth W | 1984 | J Nutr1984，114，： | 1 |
| 5 | An randomized factorial trial assessing early oral captopril,oral mononi trate,and intravenous magnesium sulphate in 58 050 patients with suspect-ed acute myocardial infarction显示文摘 | | 1995 | Lancet1995，345，： | 1 |
| 6 | The contamination of former iron and steel works sites显示文摘 | HARBER A J FORTH R A | 2001 | Environmental Geology2001，40，3： | 1 |
| 7 | J Bone Joint Surg 显示文摘 | Spangehl MJ Masri BA Oconnell JX Duncan CP et aL Prospec-tire analysis of preoperative and intraoperative investigations forthe diagnosis if infection at the sites of two hundred and tworeri-sion total hip arthroplasties | 1999 | 81 -A :672 -831999，81，： | 1 |
| 8 | Synthesis and Characterization of Liquid Crystal with Lower Temperature of Phase Change of meso-tetra-(4-n-lauroyloxyphenyl)porphyrin显示文摘The synthesis and characterization of meso-tetra (4-n-lauroyloxy phenyl) porphyrinwith long ester chains are reported. The domains of stability and the structure of the liquidcrystalline phases are determined by optical microscopy and differential scanning calorimerials(DSC). | Wei LIU Tong Shun SHI(1 Department of chemistry, The Forth Military Medical University, Xian 7100322 Department of chemistry, Jilin University. Changchun 130023) | 1999 | Chinese Chemical Letters1999，10，9： | 1 |
| 9 | Barley beta-glucans,their role in malting and brewing显示文摘 | Bam forth C W | 1982 | Brewing Digest1982，57，： | 1 |
| 10 | Patient understanding of e- mergency department discharge instructions:where are knowledge def- icits greatest? 显示文摘 | Engel KG Buckley BA Forth VE | 2012 | Acad Emerg Med2012，19，9： | 1 |
| 11 | Modeling chloride diffusion in concrete -effect of fly ash and slag显示文摘 | Thomas M D A Bam forth H P B | 1999 | Cement and Concrete Research1999，29，4： | 1 |
| 12 | Barley heta-glucans, their role in mahing and brewing显示文摘 | Bam forth C W | 1982 | Brewing Digest1982，57，： | 1 |
| 13 | Effect of As2O3 on gluconeogenesis显示文摘 | L. Szinicz W. Forth | 1988 | Archives of Toxicology1988，，6： | 1 |
| 14 | EASL-ALEH clinical practice guidelines:Non-invasive tests for evalua- tion of liver disease severity and prognosis显示文摘 | European Association forthe Study of the Liver Asociacion Latinoamericana parael Estudiodel Higado | 2015 | Journal of Hhepatology2015，63，1： | 1 |
| 15 | Iron absorption显示文摘 | Forth W Rummel W | 1973 | Physiol Rev1973，53，： | 1 |
| 16 | Modelling shrinkage of concrete from moisture lost using moisture diffusion theory显示文摘 | MU R FORTH J P | 2009 | Magazine of Concrete Research2009，61，7： | 1 |
| 17 | Arteriosclerotic aneurysm of an aberrant right subclavian artery显示文摘 | Gomes MM Bernatz PE Forth RJ | 1968 | CHEST Journal1968，54，： | 1 |
| 18 | Pyke KA: The suffuha mutation in tomato reveals a novel method of plastid replication during fruit ripening显示文摘 | Forth D | 2006 | The Journal of Experimental Botany2006，57，9： | 1 |
| 19 | Fatigue crack growth per- formance of peened friction stir welded 2195 aluminum alloy joints at elevated and cryogenic temperatures 显示文摘 | Hatamleh O Hill M Forth S | 2009 | Mat- erials Science and Engineering A2009，519，12： | 1 |
| 20 | Barley beta glucans, their role in malting and brewing显示文摘 | Barn Forth C W | 1982 | Brewing Digest1982，57，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员