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| 1 | Low-level laser therapy enhances the number of osteocytes in calvaria bone defects of ovariectomized rats显示文摘Background : Osteoporosis can make bone repair difficult. Low-level laser therapy( LLLT) has been shown to be a promising tool for bone neoformation. This study aimed to analyze the effect of LLLT on calvaria bone defects of ovariectomized rats using stereology. Methods : Fifty-four Wistar rats were subjected to bilateral ovariectomy, and bone defects were created in calvaria after 150 days. The animals were divided into nine groups(n = ?6 per group), and 24 hours after the bone defects were created they received three, six or 12 sessions of LLLT at 0, 20 or 30 J/cm 2, using a 780-nm low-intensity GaAlAs laser. One-way ANOVA followed by Tukey ' s post hoc test was used for data processing. A difference of P < 0.05 was considered statistically significant. The parameters evaluated were osteocyte density( Nv_(ost)), total osteocyte number( Nto ost), trabecular surface density( Sv_t), and trabecular surface area( Sa_t). Results : Data obtained showed that Nto ost, Sv t, and Sa t in group G2 rats were significantly different from G1(0 J/cm^2)( P < 0.05). Compared to group G4, G5 presented higher values for the parameters Sv t and Sa t, and G6 presented significantly higher values for almost all the analyzed parameters( Nv _(ost), Nto_(ost), Sv_t, and Sa t)( P < 0.05). Compared to group G7, G8 showed a higher value only for the parameter Sa t, and G9 showed significantly higher values for parameters Nv ost, Nto ost, Sv_t, and Sa_t. Conclusion : We conclude that LLLT stimulated bone neoformation and contributed to an increase in the total number of osteocytes, especially with a laser energy density of 30 J/cm^2 given for six and 12 sessions. | Priscilla Hakime Scalize Luiz Gustavo de Sousa Lígia Maria Napolitano Goncalves Dimitrius Leonardo Pitol Marcelo Palinkas Antonio Augusto Coppi Mariah Acioli Righeti Vitória Ricardo Karina Fittipaldi Bombonato-Prado Simone Cecílio Hallak Regalo Selma Siessere | 2019 | Animal Models and Experimental Medicine2019，2，1： | 2 |
| 2 | Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells显示文摘AIM: To study the therapeutic effect of three tubeguides with electrical conductivity associated to mesenchymal stem cells(MSCs) on neuro-muscular regeneration after neurotmesis.METHODS: Rats with 10-mm gap nerve injury were tested using polyvinyl alcohol(PVA), PVA-carbon nanotubes(CNTs) and MSCs, and PVA-polypyrrole(PPy). The regenerated nerves and tibialis anterior muscles were processed for stereological studies after 20 wk. The functional recovery was assessed serially for gait biomechanical analysis, by extensor postural thrust, sciatic functional index and static sciatic functionalindex(SSI), and by withdrawal reflex latency(WRL). In vitro studies included cytocompatibility, flow cytometry, reverse transcriptase polymerase chain reaction and karyotype analysis of the MSCs. Histopathology of lung, liver, kidneys, and regional lymph nodes ensured the biomaterials biocompatibility. RESULTS: SSI remained negative throughout and independently from treatment. Differences between treted groups in the severity of changes in WRL existed, showing a faster regeneration for PVA-CNTs-MSCs(P < 0.05). At toe-off, less acute ankle joint angles were seen for PVA-CNTs-MSCs group(P = 0.051) suggesting improved ankle muscles function during the push off phase of the gait cycle. In PVA-PPy and PVA-CNTs groups, there was a 25% and 42% increase of average fiber area and a 13% and 21% increase of the 'minimal Feret's diameter' respectively. Stereological analysis disclosed a significantly(P < 0.05) increased myelin thickness(M), ratio myelin thickness/axon diameter(M/d) and ratio axon diameter/fiber diameter(d/D; g-ratio) in PVA-CNT-MSCs group(P < 0.05). CONCLUSION: Results revealed that treatment with MSCs and PVA-CNTs tube-guides induced better nerve fiber regeneration. Functional and kinematics analysis revealed positive synergistic effects brought by MSCs and PVA-CNTs. The PVA-CNTs and PVA-PPy are promising scaffolds with electric conductive properties, bio- and cytocompatible that might prevent the secondary neurogenic muscular atrophy by improving the reestablishment of the neuro-muscular junction. | Jorge Ribeiro Tiago Pereira Ana Rita Caseiro Paulo Armada-da-Silva Isabel Pires Justina Prada Irina Amorim Sandra Amado Miguel Franca Carolina Goncalves Maria Ascensao Lopes Jose Domingos Santos Dina Morais Silva Stefano Geuna Ana Lúcia Luís Ana Colette Maurício | 2015 | World Journal of Stem Cells2015，7，6： | 2 |
| 3 | Four-dimensional ultrasonography of the fetal heart with spatiotemporal image correlation显示文摘 | Goncalves LF Lee W Chaiworapongsa T et a l | 2003 | Am J Obstet Gynecol2003，189，6： | 1 |
| 4 | Effects of a single intracoronary injection of basic fibroblast growth factor in stable angina pectoris显示文摘 | Unger E F Goncalves L Epstein S E | 2000 | Am J Cardiol2000，85，12： | 1 |
| 5 | Preparation of conducting polyanilines doped with Keggin - type polyoxometalates and their application as counter electrode in dye - sensitized solar cells显示文摘 | ALMEIDA L GONCALVES A BENEDETTI J MIRANDA P PASSONI L NOGUEIRA A | 2010 | J Mater Sci2010，45，18： | 1 |
| 6 | The inhibit of epidural morphine -inducepruitus by epidural droperidol 显示文摘 | Horta ML Ramos L Goncalves ZR | 2000 | Anesth analg2000，90，3： | 1 |
| 7 | Quality changes of deepwater pink shrimp (parapenaeus longirostris) packed in mod- ified atmosphere显示文摘 | Goncalves A C Lopez-caballero M E Nunes M L | 2003 | Journal of Food Science2003，68，8： | 1 |
| 8 | On the growth and electrical characterization of CuO nanowires by thermal oxidation显示文摘 | Goncalves A M B Campos L C Ferlaut A S | 2009 | J Appl Phys2009，106，03： | 1 |
| 9 | Solvent recovery from soybean oil/hexane mis- celia by polymeric membranes 显示文摘 | RIBEIRO A P B DE MOUR J M L N GONCALVES L A G | 2006 | Journal of Membrane Science2006，282，12： | 1 |
| 10 | A method for the evaluation of the fatigue operational life of submarine pressure hulls 显示文摘 | Robles L B R Buelta M A Goncalves E | 2000 | International Journal of Fatigue2000，22，： | 1 |
| 11 | Biology of two parasitoids of leafminers Liriomyza spp,Diglyphus isaea and Dpoppoea,in southern Portugal显示文摘 | GONCALVES M A ALMEIDA L | 2005 | Journal of Food Agriculture & Environment2005，3，2： | 1 |
| 12 | Clinical and labora-tory features in acute generalized pustular psoriasis : a retrospectivestudy of 34 patients显示文摘 | Borges-Costa J Silva R Goncalves L | 2011 | Am J Clin Dermatol2011，12，4： | 1 |
| 13 | Sulfate reduction in acidogenic phase anaerobic-digestion 显示文摘 | REIS M A M GONCALVES L M D CARRONDO M J T | 1988 | Water Science and Technology1988，20，1112： | 1 |
| 14 | Effect of Lactobacillus delbrueckii on cholesterol metabolism in germ-free mice and on atherogenesis in apolipoprotein E knock-out mice显示文摘 | Portugal L R Goncalves J L Femandes L R | 2006 | Brazilian Journal of Medical and Biological Research2006，，39： | 1 |
| 15 | The utilization of diets containing high levels of fish protein hydrolysate by turbot ( Scophthalmus maximus) juveniles 显示文摘 | OLIVA-TELES A CERQUEIRA A L GONCALVES P | 1999 | Aquaculture1999，179，14： | 1 |
| 16 | Effect of pulp potential on the flotation of a sulfide copper ore 显示文摘 | Peres A E C Goncalves K L C Andrade V L L | 2005 | Minerals and Metallurgical Processing2005，22，3： | 1 |
| 17 | Applications of 2-dimensional matrix array for 3-and4- dimensional examination of the fetus :a pictorial essay 显示文摘 | Goncalves L F Espinoza l | 2006 | J Ultrasound Med2006，25，6： | 1 |
| 18 | The effect of grinding conditions on the flotation of a sulphide copper ore 显示文摘 | Goncalves K L C Andrade V L L Peres A E C | 2003 | Minerals Engineering2003，16，11： | 1 |
| 19 | Correlation between risk aversion and wealth distribution显示文摘 | Iglesias J R Goncalves S Vega J L | 2004 | Physica A2004，342，6： | 1 |
| 20 | Validation and uti lization of PCR for differential diagnosis and prevalence deter mination of Entamoeba histolytica/Entamoeba dispar in Salva dor City,Brazil显示文摘 | Santos F L Goncalves Mde S Soares N M | 2011 | Braz J Infect Dis2011，15，2： | 1 |
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