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| 1 | A gene expression estimator of intramuscular fat percentage for use in both cattle and sheep显示文摘Background:The expression of genes encoding proteins involved in triacyglyceride and fatty acid synthesis and storage in cattle muscle are correlated with intramuscular fat(IMF)%.Are the same genes also correlated with IMF%in sheep muscle,and can the same set of genes be used to estimate IMF%in both species?Results:The correlation between gene expression(microarray) and IMF%in the longissimus muscle(LM) of twenty sheep was calculated.An integrated analysis of this dataset with an equivalent cattle correlation dataset and a cattle differential expression dataset was undertaken.A total of 30 genes were identified to be strongly correlated with IMF%in both cattle and sheep.The overlap of genes was highly significant,8 of the 13 genes in the TAG gene set and 8 of the 13 genes in the FA gene set were in the top 100 and 500 genes respertively most correlated with IMF%in sheep,P-value = 0.Of the 30 genes,CIDEA,THRSP,ACSM1,DGAT2 and FABP4 had the highest average rank in both species.Using the data from two small groups of Brahman cattle(control and Hormone growth promotant-treated[known to decrease IMF%in muscle]) and 22 animals in total,the utility of a direct measure and different estimators of IMF%(ultrasound and gene expression) to differentiate between the two groups were examined.Directly measured IMF%and IMF%estimated from ultrasound scanning could not discriminate between the two groups.However,using gene expression to estimate IMF%discriminated between the two groups.Increasing the number of genes used to estimate IMF%from one to five significantly increased the discrimination power;but increasing the number of genes to 15 resulted in little further improvement.Conclusion:We have demonstrated the utility of a comparative approach to identify robust estimators of IMF%in the LM in cattle and sheep.We have also demonstrated a number of approaches(potentially applicable to much smaller groups of animals than conventional methods) to using gene expression to rank animals for IMF%within a single farm/treatment,or to estimate differences in IMF%between two farms/treatments. | Bing Guo Kritaya Kongsuwan Paul L Greenwood Guanghong Zhou Wangang Zhang Brian P Dalrymple | 2014 | Journal of Animal Science and Biotechnology2014，5，4： | 6 |
| 2 | Microbiological study of cooked crustaceans and molluscan shellfish from UK production and retail establishments显示文摘 | Little C L Greenwood M | 2007 | Int J Environ Health Res2007，17，3： | 1 |
| 3 | Statin therapy and autoimmune disease:from protein prenylation to immunomodulation显示文摘 | Greenwood J Steinman L Zamvil SS | | 0，，： | 1 |
| 4 | An alternative to the Maugis model of adhesion between elastic spheres 显示文摘 | GREENWOOD J A JOHNSION K L | 1998 | Journal of Physics: D1998，31，： | 1 |
| 5 | Microbiological study of cooked crustaceans and molluscan shellfish from UK production and retail establishments显示文摘 | Sagoo S K Little C L Greenwood M | 2007 | Int J Environ Health Res2007，17，3： | 1 |
| 6 | An adhesion map for contact of elastic spheres 显示文摘 | JOHNSON K L GREENWOOD J A | 1997 | Journal of Colloid and Interface Science1997，192，： | 1 |
| 7 | Management of young goats during prolonged fasting affects carcass characteristics but not pre-slaughter live weight or cortisol显示文摘 | Greenwood P L Finn J A May T J Nicholls P J | | 0，，06： | 1 |
| 8 | Short communication: Supplementing grape marc to cows fed a pasture-ba diet as a met- hod to alter nitrogen partitioning and excretion 显示文摘 | Greenwood S L Redwards G Harrison R | 2014 | Dairy Sci2014，95，： | 1 |
| 9 | Effect of chronic irradiation on the humeral immune response of a marine fish, the eel pout (Zoarces viviparous L)显示文摘 | GREENWOOD L N KNQWLES J F | 1995 | International Journal of Radiation Biology1995，67，1： | 1 |
| 10 | Atomic spectrometry update: atomic mass spectrometry 显示文摘 | BACON J R GREENWOOD J C VAECK L V | 2003 | Analytical Atomic Spectrometry2003，11，4： | 1 |
| 11 | Statin therapy and autoimmune disease:from protein prenylation to immunomodulation显示文摘 | Greenwood J Steinman L Zamvil SS | 2006 | Nat Rev Immunol2006，6，： | 1 |
| 12 | Removing gasoline sulfur显示文摘 | MEIER P F REED L E GREENWOOD G J | 2001 | Hydrocarbon Engineering2001，，6： | 1 |
| 13 | Assessment of the microbiological safety of dried spices and herbs from production and retail premises in the United Kingdom显示文摘 | SAGOO S K LITTLE C L GREENWOOD M | 2013 | Food Microbiology2013，26，： | 1 |
| 14 | Displacement damage cross section for neutron-I rradiated silicon carbide显示文摘 | Heinisch H L Greenwood L R | 2004 | J of NUCl Mater2004，327，： | 1 |
| 15 | Neutron interaction and atomic recoil spectra显示文摘 | Greenwood L R | 1994 | J of Nucl Mater1994，216，： | 1 |
| 16 | Pre-slaughter management practices influence carcass characteristics of young goats显示文摘 | Greenwood P L Finn J A May T J Nicholls P J | | 0，，07： | 1 |
| 17 | Comparison of the health belief model and the theory of planned behaviour in the prediction of dieting and fasting behaviour显示文摘 | Nejad L Wertheim E Greenwood K | 2005 | E-journal of Applied Psychology2005，1，1： | 1 |
| 18 | Sleeping patternsduring pregnancy in Japanese women 显示文摘 | Suzuki S Dennerstein L Greenwood K M | 1994 | J Psychosom ObstetGynaecol1994，15，1： | 1 |
| 19 | Comparison of the generic neuronal differentiation and neuron subtype specification functions of mammalian achaete-scute and atonal homologs in cultured neural progenitor cells显示文摘 | Lo L Dormand E Greenwood A | 2002 | Development2002，129，： | 1 |
| 20 | Root characteristics of temperate pasture in New South Wales after grazing at three stocking rates for 30 years 显示文摘 | GREENWOOD K L HUCHINSON K J | 1999 | Grass and Forage Science1999，53，2： | 1 |
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