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| 1 | Endoscopic submucosal dissection in dogs in a World Gastroenterology Organisation training center显示文摘AIM: To evaluate if canine models are appropriate for teaching endoscopy fellows the techniques of endoscopic submucosal dissection (ESD). METHODS: ESD was performed in 10 canine models under general anesthesia, on artificial lesions of the esophagus or stomach marked with coagulation points. After ESD, each canine model was euthanized and surgical resection of the esophagus or stomach was carried out according to 'The Principles of Humane Experimental Technique, Russel and Burch'. The ESD specimens were fixed with needles on cork submerged in a formol solution with the esophagus or stomach, and delivered to the pathology department to be analyzed. RESULTS: ESD was completed without complications using the Hook-knife in five esophageal areas, with a procedural duration of 124 ± 19 min, a length of 27.4 ± 2.6 mm and a width of 21 ± 2.4 mm. ESD was also completed without complications using the IT-knife2 in five gastric areas, with a procedural duration of 92.6 ± 19 min, a length of 32 ± 2.5 mm and a width of 18 ± 3.7 mm. CONCLUSION: ESD is feasible in the normal esopha- gus and stomach of canine models, which are appropriate for teaching this technique. | Miguel A Tanimoto Gonzalo Torres-Villalobos Rikiya Fujita Patricio Santillan-Doherty Jorge Albores-Saavedra German Gutierrez Luis A Martin-del-Campo Carlos Bravo-Reyna Octavio Villanueva Jose J Villalobos Misael Uribe Miguel A Valdovinos | 2010 | World Journal of Gastroenterology2010，16，14： | 7 |
| 2 | Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate显示文摘An in vitro gas production(GP) technique was used to investigate the effects of combining different doses of Salix babylonica extract(SB) with exogenous fibrolytic enzymes(EZ) based on xylanase(X) and cellulase(C), or their mixture(XC; 1:1 v/v) on in vitro fermentation characteristics of a total mixed ration of corn silage and concentrate mixture(50:50, w/w) as substrate. Four levels of SB(0, 0.6, 1.2 and 1.8 m L g–1 dry matter(DM)) and four supplemental styles of EZ(1 μL g–1 DM; control(no enzymes), X, C and XC(1:1, v/v) were used in a 4×4 factorial arrangement. In vitro GP(m L g–1 DM) were recorded at 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 h of incubation. After 72 h, the incubation process was stopped and supernatant p H was determined, and then filtered to determine dry matter degradability(DMD). Fermentation parameters, such as the 24 h gas yield(GY24), in vitro organic matter digestibility(OMD), metabolizable energy(ME), short chain fatty acid concentrations(SCFA), and microbial crude protein production(MCP) were also estimated. Results indicated that there was a SB?EZ interaction(P<0.0001) for the asymptotic gas production(b), the rate of gas production(c), GP from 6 to 72 h, GP2(P=0.0095), and GP4(P=0.02). The SB and different combination of enzymes supplementation influenced(P<0.001) in vitro GP parameters after 12 h of incubation; the highest doses of SB(i.e., 1.8 m L g–1 DM), in the absence of any EZ, quadratically increased(P<0.05) the initial delay before GP begins(L) and GP at different incubation times, with lowering b(quadratic effect, P<0.0001) and c(quadratic effect, P<0.0001; linear effect, P=0.0018). The GP was the lowest(P<0.05) when the highest SB level was combined with cellulose. There were SB?EZ interactions(P<0.001) for OMD, ME, the partitioning factor at 72 h of incubation(PF72), GY24, SCFA, MCP(P=0.0143), and p H(P=0.0008). The OMD, ME, GY24 and SCFA with supp lementation of SB extract at 1.8 m L g–1 DM were higher(P<0.001) than the other treatments, however,PF72 was lower(quadratic effect, P=0.0194) than the other levels. Both C and X had no effect(P>0.05) on OMD, p H, ME, GY24, SCFA and MP. The combination of SB with EZ increased(P<0.001) OMD, ME, SCFA, PF72 and GP24, whereas there was no impact on p H. It could be concluded that addition of SB extract, C, and X effectively improved the in vitro rumen fermentation, and the combination of enzyme with SB extract at the level of 1.2 m L g–1 was more effective than the other treatments. | Abdelfattah Z M Salem German Buendía-Rodríguez Mona M M Elghandour María A Mariezcurrena Berasain Francisco J Pea Jiménez Alberto B Pliego Juan C V Chagoyán María A Cerrillo Miguel A Rodríguez | 2015 | Journal of Integrative Agriculture2015，14，1： | 4 |
| 3 | Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion显示文摘Development of a novel in vivo lung perfusion(IVLP)procedure allows localized delivery of high-dose doxorubicin(DOX)for targeting residual micrometastatic disease in the lungs.However,DOX delivery via IVLP requires careful monitoring of drug level to ensure tissue concentrations of this agent remain in the therapeutic window.A small dimension nitinol wire coated with a sorbent of biocompatible morphology(Bio-SPME)has been clinically evaluated for in vivo lung tissue extraction and determination of DOX and its key metabolites.The in vivo Bio-SPME-IVLP experiments were performed on pig model over various(150 and 225 mg/m^(2))drug doses,and during human clinical trial.Two patients with metastatic osteosarcoma were treated with a single 5 and 7 μg/mL(respectively)dose of DOX during a 3-h IVLP.In both pig and human cases,DOX tissue levels presented similar trends during IVLP.Human lung tissue concentrations of drug ranged between 15 and 293 μg/g over the course of the IVLP procedure.In addition to DOX levels,Bio-SPME followed by liquid chromatography-mass spectrometry analysis generated 64 metabolic features during endogenous metabolite screening,providing information about lung status during drug administration.Real-time monitoring of DOX levels in the lungs can be performed effectively throughout the IVLP procedure by in vivo Bio-SPME chemical biopsy approach.Bio-SPME also extracted various endogenous molecules,thus providing a real-time snapshot of the physiology of the cells,which might assist in the tailoring of personalized treatment strategy. | Barbara Bojko Nikita Looby Mariola Olkowicz Anna Roszkowska Bogumiła Kupcewicz Pedro Reck dos Santos Khaled Ramadan Shaf Keshavjee Thomas K.Waddell German Goomez-Rios Marcos Tascon Krzysztof Gorynski Marcelo Cypel Janusz Pawliszyn | 2021 | Journal of Pharmaceutical Analysis2021，11，1： | 3 |
| 4 | Relative deficiency in IgA production by duodenal explants from german shepherd dogs with small intestinal Disease显示文摘 | GERMAN A J HALL E J DAYM J | 2000 | Vet Immunol Immunopatho2000，76，12： | 1 |
| 5 | 查看详情显示文摘 | Brouwer H D Schellekens M A J Klumperman B Monteiro M J German A L | | 0，，： | 1 |
| 6 | Acid-sensing ion channels (ASICs) in the taste buds of adult zebrafish显示文摘 | E. Vi?a V. Parisi R. Cabo R. Laurà S. López-Velasco A. López-Mu?iz O. García-Suárez A. Germanà J.A. Vega | 2013 | Neuroscience Letters2013，，： | 1 |
| 7 | Effect of lubrication mode and compaction temperature on the properties of Fe-Ni-Cu-Mo-C显示文摘 | Degoix C N Griffo A German R M | 1998 | The International Journal of powder Metallurgy1998，34，2： | 1 |
| 8 | Hydrothermal scavenging of rare earthelements in the ocean 显示文摘 | German C R Klinkhammer G P Edmond J M Mura A Elderfield H | 1990 | Nature1990，345，： | 1 |
| 9 | Personalmetabolomics as a next generation nutritional assessment 显示文摘 | GERMAN J B ROBERTS M A WATLDNS S M | 2003 | J Nutr2003，133，12： | 1 |
| 10 | Tospovirus-- thrips interactions 显示文摘 | WHITFIELD A E ULLMAN D E GERMAN T L | 2005 | Annual Review of Phytopathology2005，43，： | 1 |
| 11 | Effect of lubrication mode and compaction temperature on the properties of Fe-Ni-Cu-Mo-C显示文摘 | GRIF C A GERMAN R M | 1998 | The International Journal of Powder Metallurgy1998，34，2： | 1 |
| 12 | Reasoning about Systems with Many Processes 显示文摘 | S German A P Sistla | 1992 | Journal of the ACM (S0004-5411)1992，39，： | 1 |
| 13 | Global identification of microRNA-target RNA pairs by parallel analysis of RNA ends 显示文摘 | German M A Pillay M Jeong D H | 2008 | Nature Biotechnotogy2008，26，8： | 1 |
| 14 | The electronic factor for outer-sphere electron-transfer reactions显示文摘 | Kuznetsov A M | 1985 | J Chem Soc Faraday Trans I1985，81，： | 1 |
| 15 | Copolymer of ethylene and methyl acrylate at low pressure determination of the kinetic by sequential sampling 显示文摘 | German A L Heikenks D | 1971 | J Polym Sci Part A1971，9，8： | 1 |
| 16 | Does subarachnoid blood extravasation per se induce long-term neuropsychological and cog- nitive alterations 显示文摘 | Germans A Caruso G Caffo M | 1998 | Acta Neurochir ( Wien )1998，140，8： | 1 |
| 17 | Construction of Parallel A- nalysis of RNA Ends ( PARE ) libraries for the study of cleaved miRNA targets and the RNA degradome显示文摘 | German M A Luo S Schroth G | 2009 | Nature Protoeols2009，4，3： | 1 |
| 18 | An inference system for a fuzzy logic on the basis of multi-valued Lukasiewicz calculi显示文摘 | GEMAN O V SAMKO R A GERMAN Yu O | 2010 | Works of the Belarusian University of Technology(Minsk Belarus) Natural Sciences and Informatics VI2010，，18： | 1 |
| 19 | Centrosome abnormalities and chromosome instability occur together in pre-invasive carcinomas显示文摘 | German A P Jan W Yening Z | 2003 | Cancer Res2003，63，6： | 1 |
| 20 | Testing community velocity models for southern california using the ambient seismic field显示文摘 | Shuo M German A P Gregory C B | 2008 | Bulletin of the Seismological Society of America2008，98，6： | 1 |
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