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| 1 | Human umbilical cord mesenchymal stem cells as treatment of adjuvant rheumatoid arthritis in a rat model显示文摘AIM:To investigate the effect of human umbilical cord stem cells,both mesenchymal and hematopoietic(CD34+),in the treatment of arthritis.METHODS:Mesenchymal stem cells(MSCs) and hematopoietic(CD34+) stem cells(HSC) were isolated from human umbilical cord blood obtained from the umbilical cord of healthy pregnant donors undergoing fullterm normal vaginal delivery.MSC,HSC,methotrexate(MTX) and sterile saline were injected intra-articularly into the rat hindpaw with complete freunds adjuvant(CFA) induced arthritis after the onset of disease(day 34),when arthritis had become well established(arthritis score ≥ 2).Arthritic indices were evaluated and the levels of interleukin(IL)-1,tumor necrosis factor(TNF)-α and interferon(IFN)-γ and anti-inflammatory cytokine IL-10 in serum were determined using enzyme-linked immunosorbent assay.Animals of all groups were sacrificed 34 d after beginning treatment,except positive control(PC) which was sacrificed at 10,21 and 34 d for microscopic observation of disease progression.We used hematoxylin,eosin and Masson's trichrome stains for histopathological examination of cartilage and synovium.RESULTS:The mean arthritis scores were similar in all groups at 12 and 34 d post immunization,with no statistical significant difference.Upon the injection of stem cells(hematopoietic and mesenchymal),the overall arthritis signs were significantly improved around 21 d after receiving the injection and totally disappeared at day 34 post treatment in MSC group.Mean hindpaw diameter(mm) in the MSC rats was about half that of the PC and MTX groups(P = 0.007 and P = 0.021,respectively) and 0.6 mm less than the HSC group(P = 0.047),as indicated by paw swelling.Associated with these findings,serum levels of TNF-α,IFN-γ and IL-1 decreased significantly in HSC and MSC groups compared to PC and MTX groups(P < 0.05),while the expression of IL-10 was increased.Histopathological examination with H and E stain revealed that the MTX treated group showed significant reduction of leucocytic infiltrate and hypertrophy of the synovial tissue with moderate obliteration of the joint cavity.Stem cells treated groups(both hematopoietic CD34+ and mesenchymal),showed significant reduction in leucocytic infiltrate and hypertrophy of the synovial tissue with mild obliteration of the joint cavity.With Masson's trichrome,stain sections from the PC group showed evidence of vascular edema of almost all vessels within the synovium in nearly all arthritic rats.Vacuoles were also visible in the outer vessel wall.The vessel became hemorrhagic and finally necrotic.In addition,there was extensive fibrosis completely obliterating the joint cavity.The mean color area percentage of collagen in this group was 0.324 ± 0.096,which was significantly increased when compared to the negative control group.The mean color area percentage of collagen in hematopoietic CD34+ and mesenchymal groups was 0.176 ± 0.0137 and 0.174 ± 0.0197 respectively,which showed a marked decrement compared to the PC group,denoting a mild increase in synovial tissue collagen fibers.CONCLUSION:MSC enhance the efficacy of CFAinduced arthritis treatment,most likely through the modulation of the expression of cytokines and amelioration of pathological changes in joints. | Sahar Greish Noha Abogresha Zeinab Abdel-Hady Eman Zakaria Mona Ghaly Mohamed Hefny | 2012 | World Journal of Stem Cells2012，4，10： | 17 |
| 2 | Human CD34' stem cells promote healing of diabetic foot ulcers in rats显示文摘 | Elsharawy MA Naim M Greish S | 2012 | Interact Cardiovasc Thorac Surg2012，14，3： | 1 |
| 3 | Enhanced permeability and retention of macromolecular drugs in solid tumors:A royal gate for targeted antieaneer nanomedicines 显示文摘 | GREISH K | 2007 | J Drug Target2007，15，78： | 1 |
| 4 | SMA-doxorubicin, a new polymeric micellar drug for effective targeting to solid tumors显示文摘 | Greish K Sawa T Fang J | 2004 | J Control Release2004，97，： | 1 |
| 5 | Low temperature formation of hydroxyapatite-poly(alkyloxybenzoate)phosphazene composites for biomedical applications显示文摘 | Greish Y E Bender J D Lakshmi S | 2005 | Biomaterials2005，26，： | 1 |
| 6 | Forma- tion and properties of composites comprised of calcium-defi- cient hydroxyapatites and ethyl alanate polyphosphazenes 显示文摘 | GREISH Y E STURGEON J L SINGH A | 2008 | J Mater Sci-Mater Med2008，19，9： | 1 |
| 7 | Enhanced permeability and retention of macromolecular drugs in solid tumors: A royal gate for targeted anticancer nanomedicines 显示文摘 | Greish K | 2007 | Journal of Drug Targeting2007，15，78： | 1 |
| 8 | In vivo methods of nanotoxicology显示文摘 | Greish K Thiagarajan G Ghandehari H | 2012 | Methods Mol Biol2012，928，： | 1 |
| 9 | SMA-doxorubicin, a new polymeric micellar drug for effective targeting to solid tumours 显示文摘 | Greish K Sawa T Fang J | 2004 | Journal of Controlled Release2004，97，2： | 1 |
| 10 | Macromolecular therapeutics, advantages and prospects with special emphasis on solid tumor targeting显示文摘 | Greish K Fang J Inutsuka T | 2003 | Clin Pharmacokinet2003，42，13： | 1 |
| 11 | Human CD34 + stem cells pro- mote healing of diabetic foot ulcers in rats显示文摘 | Mohamed A Naim EM Greish S | 2012 | Interact CardioVasc Thorac Surg2012，14，11： | 1 |
| 12 | Human CD34+ stem cells promote healing of diabetic foot ulcers in rats 显示文摘 | Elsharawy MA Naim M Greish S | 2012 | Interact Cardiovasc Thorac Surg2012，14，3： | 1 |
| 13 | Enhanced permeability and retention of macromoleeular drugs in solid tumors: a royal gate for targeted anticaneer nanomedicines显示文摘 | Greish K | 2007 | J Drug Target2007，15，： | 1 |
| 14 | Enhanced permeability and retention of macromolecular drugs in solid tumors: a royal gate for targeted anticancer nanomedicines 显示文摘 | Greish K | 2007 | J Drug Target2007，15，78： | 1 |
| 15 | Synthesis and evaluation ofpoly(styrene-co-maleic acid)micellar nanocarriers for the deliveryof tanespimycin显示文摘 | LARSON N GREISH K BAUER H | 2011 | International journal of pharmaceutics2011，420，1： | 1 |
| 16 | Polymeric micelles of zinc protoporphyrin for tumor targeted delivery based on EPR effect and singlet oxygen generation 显示文摘 | Iyer AK Greish K Seki T | 2007 | J Drug Target2007，15，78： | 1 |
| 17 | SMA-doxorubicin, a new polymeric micellar drug for effective targeting to solid tumors显示文摘 | Greish H F sawa T Fang J | 2004 | J Control Release2004，97，2： | 1 |
| 18 | Elevating blood pressure as a strategy to increase tumor targeted delivery of macromoleeular drug SMANCS: cases of advanced solid tumors显示文摘 | Nagamitsu A Greish K Maeda H | 2009 | Jpn J Clin Oneo12009，39，11： | 1 |
| 19 | SMA-doxorubicin, a new polymeric micellar drug for effective targeting to solid tumours显示文摘 | Greish K Sawa T Fang J | 2004 | Journal of Controlled Release2004，97，： | 1 |
| 20 | Enhanced permeability and retention ( EPR) effectfor anticancer nanomedicine drug targeting 显示文摘 | Greish K | 2010 | MethodsMol Biol2010，，624： | 1 |
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