|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 2 | Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte显示文摘 | Palermo G Joris H Devroey P | 1992 | Lancet1992，340，8810： | 1 |
| 3 | Effects of different hyaluronidase concentrations and mechanical procedures for cumulus cell removal on the outcome of intracytoplasmic sperm injection显示文摘 | Van H Nagy Z P Joris H | 1997 | Human Reproduction1997，12，10： | 1 |
| 4 | Pregnancies after intracytoplasmic injection of a single spermatozoon into an oocyte 显示文摘 | Palermo G Joris H Devroey P | 1992 | Lancet1992，340，1： | 1 |
| 5 | In-vitro matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection显示文摘 | DeVos A Van de Velde H Joris H | 1999 | Hum Reprod1999，14，7： | 1 |
| 6 | Time-course of oocyte activation, pronucleus formation and cleavage in human oocytes fertilized by intracytoplasmic sperm injection 显示文摘 | NAGY ZP LIU J JORIS H | 1994 | Hum Reprod1994，9，9： | 1 |
| 7 | Pregnancies after intracytoplasmic injection of single spermatozoonintoan oocyte显示文摘 | Palermo G Joris H Devroey P | 1992 | Lancet1992，340，8810： | 1 |
| 8 | Correlation between motility of tes- ticular spermatozoa, testicular histology and the outcome of intracyto- plasmic sperm injection 显示文摘 | Nagy ZP Joris H Verheyen G | 1998 | Hum Reprod1998，13，4： | 1 |
| 9 | Early application of CPAP in newborns with gestational age below 34 weeks lower sintubation rate and shortens oxygen therapy without altering mortality and morbidity显示文摘 | Joris H Sudre P Moessinger A | | 0，，49： | 1 |
| 10 | Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte显示文摘 | Palermo G Joris H Devroey P | 1992 | Lancet1992，340，8810： | 1 |
| 11 | Analysis of 76 total fertilization failure cycles out of 2732 intracytoplasmic sperm injection cycles 显示文摘 | Liu J Nagy Z Joris H | 1995 | Hum Reprod1995，10，： | 1 |
| 12 | Comparison of the results of human embryo biopsy and outcome of PGD after zona drilling using acid Tyrode medium or a laser显示文摘 | Joris H De VA Janssens R | 2003 | Hum Reprod2003，18，9： | 1 |
| 13 | Characteristics and applications of fast-wave gyrodevices显示文摘 | Felch K L Danly B G Jory H R | 1999 | Proc of the IEEE1999，87，5： | 1 |
| 14 | The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters显示文摘 | Nagy ZP Liu J Joris H | 1995 | Hum Reprod1995，10，5： | 1 |
| 15 | The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters显示文摘 | Nagy ZP Liu J Joris H | 1995 | Hum Reprod1995，10，3： | 1 |
| 16 | An experimental Gyro-TWT显示文摘 | SYMONS R S JORY H R HEGJ1 S J | 1981 | 1EEE Trans Microwave Theory Tech1981，29，： | 1 |
| 17 | Reduced survival after human embryo and subsequent cryopreservation显示文摘 | Joris H Van den AE De VA | 1999 | Hum Reprod1999，14，11： | 1 |
| 18 | Shrimp white spot virus in the western hemisphere 显示文摘 | Jory D E Dixon H M | 1999 | Aquaculture1999，25，： | 1 |
| 19 | Intracytoplasmic single sperm injection of 1-day-old unfertilized human oocytes显示文摘 | NAGY Z P JORIS H LIU J | | 0，，12： | 1 |
| 20 | Successful fertilization and establishment of pregnancies after intracytoplasmic sperm injection in patients with globozoospermia显示文摘 | Liu J Nagy Z Joris H | 1995 | Hum Reprod1995，10，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员