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| 1 | Protective effects of anti-ricin A-chain RNA aptamer against ricin toxicity显示文摘AIM: To investigate the therapeutic potential of an RNA ligand (aptamer) specific for the catalytic ricin A-chain (RTA), the protective effects of a 31-nucleo-tide RNA aptamer (31RA), which formed a high affi nity complex with RTA, against ricin-induced toxicity in cellbased luciferase translation and cell cytotoxicity assays were evaluated. METHODS: To test the therapeutic potential of anti-RTA aptamers in Chinese hamster ovary (CHO) AA8 cells stably transfected with a tetracycline regulat-able promoter, ricin ribotoxicity was measured using luciferase and ricin-induced cytotoxicity was ascertained by MTS cell proliferation assay with tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]. RESULTS: Inhibition of protein synthesis by ricin in CHO AA8 cells resulted in diminished luciferase activity and treatment with polyclonal antibody against degly-cosylated RTA (dgA) neutralized the inhibitory effects of ricin on luciferase activity and protected against ricin-induced cytotoxicity as measured by MTS assay. The 31RA anti-RTA aptamer inhibited the translation of luciferase mRNA in cell-free reticulocyte translation as- say. 31RA aptamer also partially neutralized the inhibitory effects of ricin on luciferase activity and partially protected against ricin-induced cytotoxicity in CHO AA8 cells. CONCLUSION: We have shown that anti-RTA RNA aptamer can protect against ricin ribotoxicity in cellbased luciferase and cell cytotoxicity assays. Hence, RNA aptamer that inhibits RTA enzymatic activity represents a novel class of nucleic acid inhibitor that has the potential to be developed as a therapeutic agent for the treatment of ricin intoxication. | Shaoan Fan Feng Wu Frank Martiniuk Martha L Hale Andrew D Ellington Kam-Meng Tchou-Wong | 2008 | World Journal of Gastroenterology2008，14，41： | 3 |
| 2 | A prospective trial of volumetric intensity-modulated arc therapy vs conventional intensity modulated radiation therapy in advanced head and neck cancer显示文摘AIM: To prospectively compare volumetric intensitymodulated arc therapy(VMAT) and conventional intensity-modulated radiation therapy(IMRT) in coverage of planning target volumes and avoidance of multiple organs at risk(OARs) in patients undergoing definitive chemoradiotherapy for advanced(stage Ⅲ or Ⅳ)squamous cell cancer of the head and neck. METHODS: Computed tomography scans of 20 patients with advanced tumors of the larynx, naso-, oroand hypopharynx were prospectively planned using IMRT(7 field) and VMAT using two arcs. Calculated doses to planning target volume(PTV) and OAR were compared between IMRT and VMAT plans. Dose-volume histograms(DVH) were utilized to obtain calculated doses to PTV and OAR, including parotids, cochlea,spinal cord, brainstem, anterior tongue, pituitary and brachial plexus. DVH's for all structures were compared between IMRT and VMAT plans. In addition the planswere compared for dose conformity and homogeneity. The final treatment plan was chosen by the treating radiation oncologist. RESULTS: VMAT was chosen as the ultimate plan in 18 of 20 patients(90%) because the plans were thought to be otherwise clinically equivalent. The IMRT plan was chosen in 2 of 20 patients because the VMAT plan produced concentric irradiation of the cord which was not overcome even with an avoidance structure. For all patients, VMAT plans had a lower number of average monitor units on average(MU = 542.85) than IMRT plans(MU = 1612.58)(P < 0.001). Using the conformity index(CI), defined as the 95% isodose volume divided by the PTV, the IMRT plan was more conformal with a lower conformity index(CI = 1.61) than the VMAT plan(CI = 2.00)(P = 0.003). Dose homogeneity, as measured by average standard deviation of dose distribution over the PTV, was not different with VMAT(1.45 Gy) or IMRT(1.73 Gy)(P = 0.069). There were no differences in sparing organs at risk.CONCLUSION: In this prospective study, VMAT plans were chosen over IMRT 90% of the time. Compared to IMRT, VMAT plans used only one third of the MUs, had shorter treatment times, and similar sparing of OAR. Overall, VMAT provided similar dose homogeneity but less conformity in PTV irradiation compared to IMRT. This difference in conformity was not clinically significant. | Simon D Fung-Kee-Fung Rachel Hackett Lee Hales Graham Warren Anurag K Singh | 2012 | World Journal of Clinical Oncology2012，3，4： | 2 |
| 3 | National beef tenderness survey-1998显示文摘 | Brooks J C Gwartney J B Hale D S | 2000 | J Anim Sci2000，78，： | 1 |
| 4 | Output characterization of frequency shifted feedback laser: theory and experiment显示文摘 | Paul D Hale Frank V Kowalski | 1990 | IEEE J QE1990，26，10： | 1 |
| 5 | Zinc alpha-2-gly- coprotein is expressed by malignant prostatic epithelium and may serve as a potential serum marker for prostate cancer 显示文摘 | Hale L P Price D T Sanchez L M | 2001 | Clin Cancer Res2001，7，4： | 1 |
| 6 | Annular honey- comb seals:Test results for leakage and rotordynam- ic coefficients;comparisons to labyrinth and smooth configurations 显示文摘 | CHILDS D ELROD D HALE K | 1989 | Journal of tribology1989，111，2： | 1 |
| 7 | Collision Induced Dissociation of Fe2^+ 显示文摘 | Loh S K Lian L Hales D A | 1988 | J Phys Chem1988，92，： | 1 |
| 8 | 显示文摘 | Morales A R Belfield K D Hales J M | 2006 | Chem Mater2006，18，： | 1 |
| 9 | Recurrent morphoeic basal carcinoma at the later canthus with orbitocranal invasion显示文摘 | Selva D Hale L Bouskill K | | 0，，02： | 1 |
| 10 | Quantifyingthe Total and Bioavailable Polycyclic Aromatic Hydrocarbons andDioxins in Biochars 显示文摘 | HALE S E LEHMAN N J RUTHERFOR D D | 2012 | Environmental Science & Technology2012，46，5： | 1 |
| 11 | FGF21 reverses hepatic steatosis, increases energy expenditure and improves insulin sensitivity in diet-induced obese mice 显示文摘 | XU Jing LLOYD D J HALE C | 2009 | Diabetes2009，58，1： | 1 |
| 12 | Assessment of Scenic Beauty of the Roadside Vegetation in Northern England显示文摘 | Akbar K F Hale W H G Headley A D | 2003 | Landscape and Urban Planning2003，，63： | 1 |
| 13 | A global review of polybrominated diphenyl ether flame retardant contamination in birds显示文摘 | Chen D Hale R C | 2010 | Environ Int2010，36，7： | 1 |
| 14 | Numerous transposed sequences of mitochondrial cytochrome oxidase I - II in aphids of the genus Sitobin (Hemiptera: Aphididae) 显示文摘 | Sunnucks P Hales D F | 1996 | Mol Biol Evol1996，13，： | 1 |
| 15 | Pudendal neuralgia显示文摘 | Khoder W Hale D | 2014 | Obstet Gynecol Clin North Am2014，41，3： | 1 |
| 16 | Fetal growth and adult diseases显示文摘 | Ozanne SE Fernandez-Twinn D Hales CN | 2004 | Semin Perinato12004，28，1： | 1 |
| 17 | Dip moveout by Fourier transform显示文摘 | HALE D | 1984 | Geophys- ics1984，49，6： | 1 |
| 18 | Carcinoma of the thyroid with a mixed medullary and follicular pat- tern: morphologic, immunohistochemical, and clinical laboratory studies显示文摘 | Hales M Rosenau W Okerlund M D | 1982 | Cancer1982，50，7： | 1 |
| 19 | A study of the potential of acetylation to improve the properties of plant fibres显示文摘 | HILL C A KHALIL S H HALE M D | 1998 | Industrial Crops and Products1998，8，1： | 1 |
| 20 | National beef tenderness survey 显示文摘 | MORGAN J B SAVELL J W HALE D S | 1991 | J Anita Sci1991，，69： | 1 |
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