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| 1 | New multi protein patterns differentiate liver fibrosis stages and hepatocellular carcinoma in chronic hepatitis C serum samples显示文摘瞄准:识别一多象用为肝纤维变性(F1-F2 ) 的察觉和区别的提高表面的激光 desorption/ionisation time-of-flight 团 spectrometry (SELDI-TOF-MS ) 的单个蛋白质标记一样的浆液蛋白质模式,在有长期的丙肝病毒(HCV ) 的病人的肝肝硬化(F4 ) andhepatocellular 癌(HCC ) 。方法:有 F1/F2 纤维变性的 39 个病人,的 Serumsamples 有 F4 纤维变性的 44 个病人,有我们重新申请到 CM10 数组并且分析了使用 SELDI-TOF ProteinChip 系统的 HCC 的 34 个病人(PBS-IIc;CiphergenBiosystems ) 在阴离子交换分别以后。所有病人 andhistologically 有长期的丙肝证实的纤维变性 stage/HCC。数据被 multivariatestatistical 技术和人工的神经网络为蛋白质模式分析。结果:4 肽 / 蛋白质 multimarkerpanel (7486, 12843, 44293 和 53598 Da ) 正确地在训练样品(AUROC0.943 ) 的 HCV 肝硬化对 HCV-HCC 的二方法比较与 100% 的敏感和 85% 的特性识别了 HCC。为 HCC 的鉴定的敏感和特性为随机的测试样品是 68% 和 80% 。肝脏硬化症的病人能被歧视有多使用 5peptide/protein 的 F1 或 F2 纤维变性的病人标记模式( 2873 , 6646 , 7775 , 10525 和 67867 Da )与100%和在训练样品( AUROC 0.976 )的85%的敏感和敏感和特性of80%的特性并且67%为随机的测试样品。有 APRI 分数 andalfa-fetopotein (法新社) 的简历标记分类器的联合改进了诊断表演。为肝纤维变性的 6646 Da 标记蛋白质作为 apolipoprotein C-I 被识别。结论:与蛋白质模式分析相结合的 SELDI-TOF-MS 技术与长期的丙肝在病人为肝肝硬化 andhepatocellular 癌的鉴定似乎是一条珍贵途径。最可能,不同浆液标记的联合将帮助以后识别肝肝硬化和早阶段的 hepatocellularcarcinomas。 | Thomas Gbel Sonja Vorderwülbecke Katarzyna Hauck Holger Fey Dieter Hussinger Andreas Erhardt | 2006 | World Journal of Gastroenterology2006，12，47： | 21 |
| 2 | Identification of osteopontin as the most consistently over-expressed gene in intrahepatic cholangiocarcinoma: Detection by oligonucleotide microarray and real-time PCR analysis显示文摘AIM: To investigate the molecular pathways involved in human cholangiocarcinogenesis by gene expression profiling. METHODS: Oligonucleotide arrays (Affymetrix U133A) were used to establish a specific gene expression profile of intrahepatic CCC in comparison to corresponding non- malignant liver tissue. To validate the expression values of the most overexpressed genes, RT-PCR experiments were performed. RESULTS: Five hundred and fifty-two statistically differentially expressed genes/ESTs (221 probes significantly up-regulated, 331 probes down-regulated; P < 0.05; fold change > 2; ≥ 70%) were identified. Using these data and two-dimensional cluster analysis,a specific gene expression profile was obtained allowing fast and reproducible differentiation of CCC, which was confirmed by supervised neuronal network modelling. The most consistently overexpressed gene (median fold change 33.5, significantly overexpressed in 100%) encoded osteopontin. Furthermore, an association of various genes with the histopathological grading could be demonstrated. CONCLUSION: A highly specific gene expression profile for intrahepatic CCC was identified, allowing for its fast and reproducible discrimination against non- malignant liver tissue and other liver masses. The most overexpressed gene in intrahepatic CCC was the gene encoding osteopontin. These data may lead to a better understanding of human cholangiocarcinogenesis. | Holger G Hass Oliver Nehls Juergen Jobst Andrea Frilling Ulrich Vogel Stephan Kaiser | 2008 | World Journal of Gastroenterology2008，14，16： | 4 |
| 3 | A comprehensive characterization of pancreatic ductal carcinoma cell lines: towards the establishment of an in vitro research platform显示文摘 | Bence Sipos Simone M?ser Holger Kalthoff Virag T?r?k Matthias L?hr Günter Kl?ppel | 2003 | Virchows Archiv2003，，5： | 4 |
| 4 | Randomized Comparison of Strategies for Type B Aortic Dissection: The INvestigation of STEnt Grafts in Aortic Dissection (INSTEAD) Trial显示文摘 | Christoph A. Nienaber Hervé Rousseau Holger Eggebrecht Stephan Kische Rossella Fattori Tim C. Rehders Günther Kundt Dierk Scheinert Martin Czerny Tilo Kleinfeldt Burkhart Zipfel Louis Labrousse Hüseyin Ince | 2009 | Circulation2009，，25： | 2 |
| 5 | Diagnosing brain death without a neurologist显示文摘 | Holger B Franz G | 2002 | BMJ2002，324，22： | 1 |
| 6 | Treatment of facial wounds with botulinum toxin A improves cosmetic outcome in primates显示文摘 | Gassner Holger G Sherris David A | 2000 | Plast Reconstr Surg2000，105，6： | 1 |
| 7 | Percutaneous left ventricular assist devices in acute myocardial infarction complicated by cardiogenic shock显示文摘 | HOLGER T SMALI ING R W SCHUI ER G C | 2007 | Eur HeartJ2007，17，： | 1 |
| 8 | Polymer microfabrication technologies for microfluidic systems显示文摘 | Holger Becker Claudia G?rtner | 2008 | Analytical and Bioanalytical Chemistry2008，，1： | 1 |
| 9 | Hyperbranched polylactide copolymers 显示文摘 | Holger F Carsten G | 2006 | Macromoleeules2006，39，： | 1 |
| 10 | Corporate Governance, Product Market Competition, and Equity Prices 显示文摘 | Xavier G Holger M M | 2011 | Journal of Finance2011，66，2： | 1 |
| 11 | Expression of Lymphangiogenic Factors and Evidence of Intratumoral Lymphangiogenesis in Pancreatic Endocrine Tumors显示文摘 | Bence Sipos Wolfram Klapper Marie-Luise Kruse Holger Kalthoff Dontscho Kerjaschki Günter Kl?ppel | 2004 | The American Journal of Pathology2004，，4： | 1 |
| 12 | Vascular endothelial growth factor(VEGF) induces remodeling and enhances TH2 - mediated sensitization and inflammation in the lung 显示文摘 | CHUN G L HOLGER L PETER B | 2004 | Nature Med2004，10，10： | 1 |
| 13 | Foreign direct investment, agglomerations, and demonstration effects: An empirical investigation显示文摘 | Frank Barry Holger G?rg Eric Strobl | 2003 | Review of World Economics2003，，4： | 1 |
| 14 | Value Articula- tion: a Framework for the Strategic Management of Intellectual Property 显示文摘 | James G Conley Peter M Bican Holger Ernst | 2013 | California Management Review2013，55，4： | 1 |
| 15 | Bulk soil C to N ratio as a simple measure of net N mineralization from stabilized soil organic matter in sandy arable soils显示文摘 | Günther Springob Holger Kirchmann | 2003 | Soil Biology and Biochemistry2003，，4： | 1 |
| 16 | The indicative meaning of diatoms,pollen and botanical macrofossils for the reconstruction of palaeoenvironments and sea-level fluctuations along the coast of Lower Saxony; Germany显示文摘 | Holger F Gerdes G Streif H | 2004 | Quaternary International2004，112，： | 1 |
| 17 | Botulinum toxin to minimize facial scarring显示文摘 | David A Sherris M Holger G | | 0，，01： | 1 |
| 18 | Polymer microfabrication technologies for microfluidic systems显示文摘 | B Holger C G/irtner | 2008 | Anal Bioanal Chem2008，390，1： | 1 |
| 19 | Imaging of head and neck tumors-methods: CT, spiral-CT, muhislice-spiral-CT 显示文摘 | Ulrich B Holger G Michael L | 2000 | Eur J Radiol2000，33，1： | 1 |
| 20 | DrugScore meets CoMFA: Adaptation of fields for molecular comparison (AFMoC) or how to tailor knowledge-based pair-potentials to a particular protein 显示文摘 | Holger G Gerhard K | 2002 | Journal of Medicinal Chemistry2002，45，： | 1 |
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