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| 1 | 肿瘤坏死因子及其受体在鼠失血性休克引起的急性肺损伤中的作用显示文摘目的 探讨失血性休克引起的急性肺损伤与肿瘤坏死因子 α(TNF α)在肺组织表达的相关性。方法 通过心腔穿刺对C5 7小鼠采取 30 %全血容量复制失血性休克模型。肺组织TNF α表达采用酶联免疫吸附法方法进行测定。肺组织中性粒细胞 (PMN)浸润采用数字荧光显微镜免疫荧光技术进行检测。肺微血管通透性 (肺水肿 )采用伊文氏蓝方法进行测定。结果 ( 1)虽然失血性休克仅引起肺组织TNF α水平轻度和短暂的升高 ,且肺组织中性粒细胞 (PMN)浸润时相上早于TNF α水平的升高。但在TNF基因剔除 (KO)小鼠 ,失血性休克引起的肺组织PMN浸润和肺损伤明显轻于C5 7野生型小鼠 ;( 2 )给予失血前TNFKO小鼠低水平的重组TNF α即可导致失血性休克引起肺组织PMN浸润和急性肺损伤 ;( 3)失血性休克引起的肺组织PMN浸润和急性肺损伤在p5 5TNF受体KO动物显著减轻 ,而在p75TNFKO动物则不产生影响。结论 肺组织低水平TNF α足以介导失血性休克引起的PMN浸润和急性肺损伤 ;p5 5TNF受体在失血性休克引起的肺部急性炎症反应中起主导作用。 | 宋勇 施毅 Alden H Harken Xianzhong Meng Christopher D Raeburn | 2003 | 中华医学杂志2003，83，8： | 12 |
| 2 | Induction of type Ⅱ alveolar epithelial cells apoptosis in mouse by lipopolysaccharide does not require TNF-α显示文摘To examine whether lipopolysaccharide (LPS)-induced apoptosis correlates with TNF-α release by type Ⅱ alveolar epithelial cells (AEC Ⅱ), whether TNF-α knockout (TNF KO) abrogates the induction of apoptosis by LPS and whether TNF-α is sufficient to induce apoptosis in this cell type Methods AEC Ⅱ were isolated from wild type mice and TNF KO mice Cells were stimulated with LPS or recombinant murine TNF-α for 24 h TNF-α in culture supernatant was determined by ELISA following LPS stimulation Apoptosis was determined by the terminal deoxynucleotidyl transferase end-labeling (TUNEL) assay after treatment with either LPS or TNF-α Results LPS induced apoptosis in wild type AEC Ⅱ in a concentration-dependent manner LPS-induced AEC Ⅱ apoptosis was accompanied by an 11-fold increase (from 0 073±0 065 ng/ml in control to 0 94±0 14 ng/ml in 50 μg/ml of LPS, P<0 01) in TNF-α release However, increasing concentrations (5 or 25 ng/ml) of recombinant murine TNF-α failed to induce AEC Ⅱ apoptosis In addition, apoptosis did occur in AEC Ⅱ isolated from TNF KO mice following LPS stimulation Conclusions This study confirms that LPS induces TNF-α release and apoptosis in murine AEC Ⅱ in vitro Exogenous TNF-α failed to induce AEC Ⅱ apoptosis, and apoptosis occurred following LPS stimulation in cells lacking the ability to produce TNF-α Taken together, these results suggest that LPS-induced AEC Ⅱ apoptosis occurs by a TNF-α-independent | 宋勇 施毅 Alden H Harken Xianzhong Meng | 2003 | Chinese Medical Journal2003，，4： | 4 |
| 3 | Oral Sulfonylurea Hypoglycemic Agents Prevent Ischemic Preconditioning in Human Myocardium: Two Paradoxes Revisited显示文摘 | Joseph C. Jr Cleveland Daniel R. Meldrum Brian S. Cain Anirban Banerjee Alden H. Harken | 1997 | Circulation1997，，1： | 2 |
| 4 | Lymphangiogenesis is pivotal to the trials of a successful cancer metastasis显示文摘 | Clarke JH Harken AH | 2004 | Surgery2004，135，2： | 1 |
| 5 | ICAM-1 expression deter- mines malignant potential of cancer 显示文摘 | Roland C L Harken A H Sarr M G | 2007 | Surgery2007，141，6： | 1 |
| 6 | Review article: the role of tumor necrosis fac- tor in renal ischemia-reperfusion injury显示文摘 | Donnahoo KK Shames BD Harken AH Meldrum DR | 1999 | J Urol1999，162，1： | 1 |
| 7 | Hemorrhage-induced acute lung injury is TLR-4 dependent显示文摘 | Barsness KA Arcaroli J Harken AH | 2004 | Am J Physiol Ragul Integr Comp Physiol2004，287，3： | 1 |
| 8 | Heart surgery:legend and a long look显示文摘 | Harken Dw Curis LE | 1967 | Am J Cardiol1967，19，3： | 1 |
| 9 | Lymphangiogenesis is pivotal to the trials of a successful cancer metastasis 显示文摘 | McCarter MD Clarke JH Harken AH | 2004 | Surgery2004，135，2： | 1 |
| 10 | Chlamydiae Pueumoniae activates NF-κB and AP-1 in human vascular smooth muscle and induces cellular proliferation显示文摘 | Miller SA Selzman CH Shames BD Barton HA Johnson SM Harken AH | 2000 | J Surg Res2000，90，1： | 1 |
| 11 | Hemorrhage-induced acute lung injury is TLR-4 dependent显示文摘 | Barsness KA Amamli J Harken AH | 2004 | Am J Physiol Regul Integr Comp Physiol2004，287，3： | 1 |
| 12 | Instrumentation for cardiopulmonary bypass--past, present, and future显示文摘 | Bartlett RH Harken DE | 1976 | Med Instrum1976，10，2： | 1 |
| 13 | Classification of Iowa wetlands using an airborne hyperspectral image:A comparison of the spectral angle mapper classifier and an object-oriented approach显示文摘 | HARKEN J SUGUMARAN R | 2005 | Canadian Journal of Remote Sensing2005，31，2： | 1 |
| 14 | Protein kinase C in normal and pathologic myocardial states显示文摘 | Cain BS Meldrum DR Harken AH | 1999 | J Surg Res1999，81，2： | 1 |
| 15 | Review article:the role of tumor necrosis factor in renal ischemia-reperfusion injury显示文摘 | Donnahoo KK Shames BD Harken AH | 1999 | J Urol1999，162，： | 1 |
| 16 | Review article : the role of tumor necrosis factor in renal ischemia reperfusion injury显示文摘 | Donnahoo KK Shames BD Harken AH | 1999 | J Urol1999，162，1： | 1 |
| 17 | Hemorrhage-induced acute lung injury is TLR-4 dependent显示文摘 | Barsness KA Arcaroli J Harken AH | 2004 | Am J Physiol Regul Inter Comp Physiol2004，287，3： | 1 |
| 18 | Review article:the role of tumor necrosis factor in renal ischemia-reperfusion injury显示文摘 | Donnahoo KK Shames BD Harken AH | 1999 | J Urol1999，162，1： | 1 |
| 19 | Lymphangiogenesis is pivotal to the trials of a successful cancer metastasis 显示文摘 | McCarter MD Clarke JH Harken AH | 2004 | Surgery2004，135，2： | 1 |
| 20 | Inhibition of caspase-1reduces human myocardial ischemic dysfunction via inhabition of IL-18and IL-1beta显示文摘 | Pomerantz BJ Reznikov LL Harken AH | 2001 | Proc Natl Acad sci USA2001，98，5： | 1 |
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