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| 1 | Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC. | Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard | 2012 | World Journal of Gastroenterology2012，18，26： | 12 |
| 2 | MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines显示文摘AIM:To investigate expression of microRNA(miRNA)and potential targets in chemotherapy resistant esoph-ageal cancer cell lines.METHODS:An in-vitro model of acquired chemotherapy resistance in esophageal adeno-(EAC)and squamous cell carcinoma(ESCC)cells was used,and microRNA expression profiles for cisplatin or 5-fluorouracil(5-FU)resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction(PCR).The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated.RESULTS:Chemotherapy resistant sublines were found to have specific miRNA signatures,and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line,and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent.Amongst others,miR-27b-3p,miR-193b-3p,miR-192-5p,miR-378 a-3p,miR-125a-5p and miR-18a-3p were dysregulated,consistent with negative posttranscriptional control of KRAS,TYMS,ABCC3,CBL-B and ERBB2 expression via these miRNAs.CONCLUSION:The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer. | Richard Hummel Corina Sie David I Watson Tingting Wang Alfiya Ansar Michael Z Michael Mark Van der Hoek Joerg Haier Damian J Hussey | 2014 | World Journal of Gastroenterology2014，20，40： | 8 |
| 3 | The use of biological activated carbon filtration for the removal of natural organic matter and organic micropollutants from water 显示文摘 | J P van der Hoek J A M H Hofman A Graveland | 1999 | Water Science and Technology1999，40，9： | 1 |
| 4 | Premature ovarian failure endovarian autoimmunity 显示文摘 | Hoek A Schoemaker J Drexhage HA | 1997 | Endocrin Rev1997，18，1： | 1 |
| 5 | A general fractional white noise theory and applications to finance显示文摘 | Robert Elliot J John Van Der Hoek | 2003 | Mathematical Finance2003，13，2： | 1 |
| 6 | Long term capacity of biological activated carbon filtration for organics removal 显示文摘 | Bonne P A C Hofman J A M H van der Hoek J P | 2002 | Water Supply2002，2，1： | 1 |
| 7 | Cystic nephroma, cystic partially differentiated nephroblas~ toma and cystic Wilm' s tumor in children : a spectrum with therapeutic dilemmas显示文摘 | Van Den Hoek J De Krijger R Van De Ven K | 2009 | Urol Int2009，82，： | 1 |
| 8 | Microbial diversity of a sulphide spire located in the Edmond deep-sea hydrothermal vent filed on the Central Indian Ridge 显示文摘 | Hoek J Banta A Hubter F | 2003 | Geobiology2003，1，： | 1 |
| 9 | Prevention of deep vein thrombosis following total hip replacement by low molecular weight heparinoid显示文摘 | HOEK J A NURMOHAMED M T HAMELYNCK K J | 1992 | Thromb Haemost1992，67，1： | 1 |
| 10 | Rock mechanics applied to the study of rockbursts显示文摘 | Cook N G W,Hoek E,Pretorius J P G, | 1966 | Journal of the South African Institute of Mining and1966，66，10： | 1 |
| 11 | A general fractional white noise theory and applications to finance显示文摘 | Elliot R J Hoek J | 2003 | Mathematical Finance2003，13，2： | 1 |
| 12 | Air pollution and lung cancer incidence in 17 European cohorts: prospective analyses from the European Study of Cohorts for Air Pollution Effects (ESCAPE) 显示文摘 | Raaschou-Nielsen 0 Andersen ZJ Beelen R Samoli E Stafoggia M Weinmayr G Hoffmann B Fischer P Nieuwenhuijsen MJ Brunekreef B Xun WW Katsouyanni K Dimakopoulou K Sommar J Forsberg B Modig L Oudin A Oftedal B Schwarze PE Nafstad p De Faire U Pedersen NL Ostenson CG Fratiglioni L Penell J Korek M Pershagen G Eriksen KT Serensen M Tjenneland A Ellermann T Eeftens M Peeters PH Meliefste K Wang M Bueno-de-Mesquita B Key TJ de Hoogh K Concin H Nagel G Yilier A Grioni S Krogh y Tsai MY Ricceri F Sacerdote C Galassi C Migliore E Ranzi A Cesaroni G Badaloni C Forastiere F Tamayo I Amiano P Dorronsoro M Trichopoulou A Bamia C Yineis p Hoek G | 2013 | Lancet Oncol2013，14，9： | 1 |
| 13 | The prognostic value of histology in the assessment of patients with Budd–Chiari syndrome显示文摘 | Thjon J Tang Kenneth P Batts Piet C de Groen Bart van Hoek Elizabeth B Haagsma Wim C.J Hop Harry L.A Janssen | 2001 | Journal of Hepatology2001，，3： | 1 |
| 14 | High serum uric acid as a novel risk factor for type 2 diabetes 显示文摘 | DEHGHAN A VAN HOEK M SIJBRANDS E J | 2008 | Diabetes Care2008，31，2： | 1 |
| 15 | High-resolution typing reveals distinct Chlamydia trachomatis strains in an at- risk population in Nanjing, China 显示文摘 | Born R J van den Hoek A Wang Q | 2013 | Sex Transm Dis2013，40，8： | 1 |
| 16 | Elevation of systemic Markers rehted to cardiovascular diseases in the peripheral blood of periodontitis patients显示文摘 | LOOS BG GRAANDIJK J HOEK FJ | 2000 | JPeriodontol2000，71，10： | 1 |
| 17 | Persistent acanthamoeba keratitis in a non-contact lens wearer following exposure to bird seed dust显示文摘 | Syam PP Narendran R van der Hoek J | 2005 | BrJ Ophthalmol2005，89，3： | 1 |
| 18 | The use of biological activated carbon filtration for the removal of natural organic matter and organic micropollutants from water显示文摘 | Van der Hoek J P | 1999 | Water Sci Technol1999，40，9： | 1 |
| 19 | Ethanol, oxidative stress, and cyto- kine-in-duced liver cell injury 显示文摘 | Hoek J B Pastorino J G | 2002 | Alcohol2002，27，1： | 1 |
| 20 | Premature ovarian failure and ovarian autoimmurdty 显示文摘 | Hoek A Schoemaker J Drexhage HA | 1997 | Endocr Rev1997，18，1： | 1 |
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