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| 1 | Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system显示文摘允许的技术内长的基因的特定的规定为基因功能的学习是珍贵的并且在治疗学有大潜力。我们创造了在 CRISPR 上系统,由核酸酶死者 Cas9 (dCas9 ) 蛋白质组成的二部件的 transcriptional 使活跃之物与互补顺序与 transcriptional 激活域和单个指南 RNA (sgRNAs ) 熔化了到基因倡导者。我们证明在 CRISPR 上能高效地以一种悦耳的方式在人和老鼠房间激活外长的记者基因。另外,我们证明在 vivo 的柔韧的记者基因激活能被把系统部件注入老鼠接合子完成。而且,我们证明在 CRISPR 上能激活内长的 IL1RN, SOX2,和 OCT4 基因。最有效的基因激活被对近似倡导者有约束力的 3-4 sgRNAs 的簇完成,建议他们在基因正式就职的 synergistic 行动。显著地,当指向多重基因的 sgRNAs 同时被介绍进房间时,柔韧的多路的内长的基因激活被完成。染色体宽的表示介绍表明了系统的高特性。 | Albert W Cheng Haoyi Wang Hui Yang Linyu Shi Yarden Katz Thorold W Theunissen Sudharshan Rangarajan Chikdu S Shivalila Daniel B Dadon Rudolf Jaenisch | 2013 | Cell Research2013，23，10： | 65 |
| 2 | 2型糖尿病患者服用吡格列酮的膀胱癌风险显示文摘背景
吡格列酮是一种有争议的噻唑烷二酮类口服降糖药物,可以有效降低糖化血红蛋白(HbA1c)浓度,且降低心血管事件的发生风险,但同时也与体重增加和充血性心力衰竭风险的增加有关。尽管目前数据有限,但还是有证据显示它可能还与膀胱癌患病风险的增加有关。 | Laurent Azoulay assistant professor Hui Yin statistician Kristian B Filion assistant professor Jonathan Assayag graduate student 王子贤(编译) | 2012 | 中国处方药2012，10，5： | 17 |
| 3 | The magnetic properties of Serbian loess and its environmental significance显示文摘This paper reports a loess-paleosol sequence located in the Danube River basin,Serbia,which formed at least since the latest part of the early Pleistocene,and before the paleomagnetic B/M boundary.Various magnetic parameters of the Serbian V-L1-V-S4 loess-paleosol sequence have been measured and analyzed in the Titel Loess Plateau.These parameters show a very similar magnetic behavior compared with that of the Chinese loess.There is a general positive relationship between magnetic susceptibility() and pedogenesis.The main contributors to are the magnetic grains of SP(superparamagnetic) and SD(single domain) magnetic domains,while MD(multi domain) contributes only a small percentage.The difference in between loess and paleosol mainly is caused by pedogenesis.The very fine magnetic minerals increase gradually with increasing soil development(from loess to soil),and they lead to higher.The thermomagnetic curves show thatmagnetic minerals in the loess layers are magnetite and maghemite,both providing a major contribution to.In contrast the paleosol layers mainly are composed of magnetite,with almost no or a very small amount of maghemite,as implied by a reversible thermomagnetic behavior.This indicates that pedogenic conditions during V-S3 and V-S4 strong soil development have resulted in maghemite that is no longer stable,and has been resolved or converted to other stable phase minerals.This likely indicates that soil moisture during V-S3 and V-S4 development exceeded a critical condition of maghemite stability. | LIU XiuMing LIU Zhi Lü Bin MARKOVI S B CHEN JiaSheng GUO Hui MA MingMing ZHAO GuoYong FENG Hua | 2013 | Chinese Science Bulletin2013，58，3： | 7 |
| 4 | Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein显示文摘A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum a-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA).A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents,less total assay time,and better linearity,recovery,precision,sensitivity and validity.AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA,and the results were compared with commercial electrochemiluminescence immunoassay (ECLIA) kit.The correlation coefficient between MPs-CLEIA and ELISA was obtained with R 2 0.6703;however,the correlation between MPs-CLEIA and ECLIA (R 2 0.9582) was obviously better than that between colorimetric ELISA and ECLIA (R 2 0.6866). | Qian-Yun Zhang a,b,Hui Chen a,Zhen Lin a,Jin-Ming Lin a a Beijing Key Laboratory of Microanalytical Methods and Instrumentation,Department of Chemistry,Tsinghua University,Beijing 100029,China b Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China | 2012 | Journal of Pharmaceutical Analysis2012，2，2： | 5 |
| 5 | Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation显示文摘Mesenchymal stem cell(MSC)-based treatments have shown promise for improving tendon healing and repair.MSCs have the potential to differentiate into multiple lineages in response to select chemical and physical stimuli, including into tenocytes. Cell elongation and cytoskeletal tension have been shown to be instrumental to the process of MSC differentiation. Previous studies have shown that inhibition of stress fiber formation leads MSCs to default toward an adipogenic lineage, which suggests that stress fibers are required for MSCs to sense the environmental factors that can induce differentiation into tenocytes. As the Rho/ROCK signal transduction pathway plays a critical role in both stress fiber formation and in cell sensation, we examined whether the activation of this pathway was required when inducing MSC tendon differentiation using rope-like silk scaffolds. To accomplish this, we employed a loss-of-function approach by knocking out ROCK,actin and myosin(two other components of the pathway) using the specific inhibitors Y-27632, Latrunculin A and blebbistatin, respectively. We demonstrated that independently disrupting the cytoskeleton and the Rho/ROCK pathway abolished the expression of tendon differentiation markers and led to a loss of spindle morphology. Together, these studies suggest that the tension that is generated by MSC elongation is essential for MSC teno-differentiation and that the Rho/ROCK pathway is a critical mediator of tendon differentiation on rope-like silk scaffolds. | Edward Maharam Miguel Yaport Nathaniel L Villanueva Takintope Akinyibi Damien Laudier Zhiyong He Daniel J Leong Hui B Sun | 2015 | Bone Research2015，3，3： | 4 |
| 6 | A novel method for Co(Ⅱ) and Cu(Ⅱ) analysis by capillary electrophoresis with chemiluminescence detection显示文摘Based on the fact that some metal ions can catalyze the chemiluminescence(CL)reaction of luminol with K3Fe(CN)6,a novel capillary electrophoresis CL method was developed for the determination of Co(Ⅱ)and Cu(Ⅱ).The separation was carried out with a 10 mmol/L sodium acetate solution containing 0.8 mmol/L luminol and 2.0 mmol/Lα-HIBA(adjusted to pH 4.8 by HAc solution).The post-capillary reagent was 2.0 mmol/L K3Fe(CN)6 which was adjusted to pH 13.0 by NaOH solution.Under the optimum conditions,the detection limits(S/N=3)for Co(Ⅱ)and Cu(Ⅱ)were 7.5×10-11mol/L and 7.5×10-9mol/L,with the linear range of 7.5×10-9mol/L to 1.0×10-6mol/L and 7.5×10-8mol/L to 5.0×10-5mol/L, respectively. | Xiao Ming Guo~a Xiang Dong Xu~(a,b) Hui Jing Zhang~a Yong Gang Hu~(a,*) Jie Zhang~a a Institute of Environmental Science & Engineering,Huazhong University of Science and Technology,Wuhan 430074,China b Department of Chemistry,Huazhong University of Science and Technology,Wuhan 430074,China | 2007 | Chinese Chemical Letters2007，18，9： | 3 |
| 7 | Associations between particle physicochemical characteristics and oxidative capacity: An indoor PM 10 study in Beijing, China显示文摘 | Longyi Shao Jinjuan Li Houyin Zhao Shushen Yang Hui Li Weijun Li Tim Jones Keith Sexton Kelly BéruBé | 2007 | Atmospheric Environment2007，，26： | 2 |
| 8 | 缺血性脑卒中后的中期血压变异性与临床结局的关系显示文摘本研究旨在探讨急性脑卒中患者发病7d内的中期血压变异性(blood pressure variability,BPV)与预后的关系。方法:本研究纳入873例急性缺血性脑卒中患者。通过计算发病7d内的收缩压和舒张压的变异系数来评价中期BPV。 | Wang Y Wang J Meng P Liu N Ji N Zhang G Xu B Zhou X Sun Y Xu Y Hui R He M 陈云 叶鹏 | 2018 | 中华高血压杂志2018，26，5： | 2 |
| 9 | Phylogenetic relationships of the Tropiduchidae-group(Homoptera:Fulgoroidea)of planthoppers inferred through nucleotide sequences显示文摘 | YEH W B YANG C T HUI C F | 1998 | Zool Stu1998，37，1： | 2 |
| 10 | Size distributions of maternal and fetal DNA in maternal plasma显示文摘 | Chan K C ZhangJ Hui A B Wong N Lau T K Leung T N | 2004 | Clin Chem2004，50，： | 1 |
| 11 | Identification offunctional peroxisome proliferator-responsive element inmurine fatty acid transport protein gene显示文摘 | Frohnert B I Hui T Y Bernlohr D A | 1999 | J Biol Chem1999，274，： | 1 |
| 12 | Synthesis of three diosgenyl saponins:dioscin polyphyllin D,and balanitin 7显示文摘 | DENG S J YU B HUI Y Z | 1999 | Carbohydrate Research1999，317，： | 1 |
| 13 | Goldmanlmage seg-mentation evaluation: A survey of unsupervised methods 显示文摘 | ZHANG Hui JASON E FRITTS B | 2008 | Computer Vision anti Image Understandin2008，110，2： | 1 |
| 14 | Incidence of antibiotic resistance and virulence determinants among Enterococcus italicus isolates from dairy products显示文摘 | MAIETTI L BONVINI B HUYS G | 2007 | Syst Appl Microbiol2007，30，6： | 1 |
| 15 | ELMO1 and Dock 180,a bipartite Racl guanine nucleotide exchange factor,promote human glioma cell invasion显示文摘 | Jarzynka M J Hu B Hui K M | 2007 | Cancer Res2007，67，15： | 1 |
| 16 | Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples显示文摘 | Hui Pan Lirong Song Yongding Liu Thomas B?rner | 2002 | Archives of Microbiology2002，，6： | 1 |
| 17 | The distribution and scaling of fluctuations for Hang Seng index in Hong Kong stock market显示文摘 | Wang B H Hui P M | 2001 | European Physical Journal B2001，20，4： | 1 |
| 18 | A contour-based approach to multisensor image registration显示文摘 | Hui Li Manjunath B S Mitra S K | 1995 | IEEE Transactions on Image Processing1995，4，3： | 1 |
| 19 | Effects of microstructure on fatigue crack growth behavior of Ti-6Al-2Zr-1Mo-1V ELI alloy显示文摘 | Li S K Xiong B Q Hui S X | 2008 | Materials Characterization2008，59，4： | 1 |
| 20 | A comparative assessment of th strengths of porcelain veneers for incisor teeth dependent on their design characteristics显示文摘 | Hui KK Williams B Davis EH | 1991 | Br Dent J1991，171，2： | 1 |
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