|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration显示文摘AIM:To develop a model of stress-induced senescence to study the hepatocyte senescence associated secretory phenotype(SASP).METHODS:Hydrogen peroxide treatment was used to induce senescence in the human Hep G2 hepatocyte cell line.Senescence was confirmed by cytochemical staining for a panel of markers including Ki67,p21,heterochromatin protein 1β,and senescence-associated-β-galactosidase activity.Senescent hepatocytes were characterised by gene expression arrays and quantitative polymerase chain reaction(q PCR),and conditioned media was used in proteomic analyses,a human chemokine protein array,and cell migration assays to characterise the composition and function of the hepatocyte SASP.RESULTS:Senescent hepatocytes induced classical markers of senescence(p21,heterochromatin protein1β,and senescence-associated-β-galactosidase activity);and downregulated the proliferation marker,Ki67.Hepatocyte senescence induced a 4.6-fold increase in total secreted protein(P=0.06)without major alterations in the protein profile.Senescence-induced genes were identified by microarray(Benjamini Hochbergcorrected P<0.05);and,consistent with the increase in secreted protein,gene ontology analysis revealed a significant enrichment of secreted proteins among inducible genes.The hepatocyte SASP included characteristic factors such as interleukin(IL)-8 and IL-6,as well as novel components such as SAA4,IL-32and Fibrinogen,which were validated by q PCR and/or chemokine protein array.Senescent hepatocyteconditioned medium elicited migration of inflammatory(granulocyte-macrophage colony stimulating factor,GM-CSF-derived),but not non-inflammatory(CSF-1-derived)human macrophages(P=0.022),which could contribute to a pro-inflammatory microenvironment in vivo,or facilitate the clearance of senescent cells.CONCLUSION:Our novel model of hepatocyte senescence provides insights into mechanisms by which senescent hepatocytes may promote chronic liver disease pathogenesis. | Katharine M Irvine Richard Skoien Nilesh J Bokil Michelle Melino Gethin P Thomas Dorothy Loo Brian Gabrielli Michelle M Hill Matthew J Sweet Andrew D Clouston Elizabeth E Powell | 2014 | World Journal of Gastroenterology2014，20，47： | 2 |
| 2 | Pokeweed antiviral protein inactivates pokeweed ribosomes implications for the antiviral mechanism显示文摘 | BONNESS M S READY M P IRVIN J D | 1994 | Plant J1994，5，2： | 1 |
| 3 | Fire hazards and some common polymers 显示文摘 | IRVINE D J MCCLUSKEY J A ROBINSON I M | 2000 | Polym Degrad Stab2000，67，3： | 1 |
| 4 | Argonaute slicing is required for heterochromatic silencing and spreading显示文摘 | Irvine D V Zaratigui M Tolia N H | 2006 | Science2006，313，5790： | 1 |
| 5 | Oxidation of phenolic pollutants by a lignin degrading enzyme from the white-rotfungus Phanerochaete chrysosporium显示文摘 | AITKEN M D VENKATADRI R IRVINE R L | 1989 | Water Research1989，23，4： | 1 |
| 6 | Precipitation and recovery of whey proteins显示文摘 | HILL A R IRVINE D M BULLOCK D H | 1982 | Canadian Institute of Food Science and Technology1982，15，： | 1 |
| 7 | Fire hazards and some common polymers 显示文摘 | Irvine D J McCluskey J A Robinson I M | 2000 | Polymer Degradation and Stability2000，67，： | 1 |
| 8 | Polymer latexes for cell-resistant and cell-interactive surfaces显示文摘 | BANERJEE P IRVINE D J MAYES A M | 2000 | Biomed Mater Res2000，50，3： | 1 |
| 9 | Pokeweed Antiviral Protein: Ribosome Inactivation and Therapeutic Applications显示文摘 | Irvin J D Uckun F M | 1992 | Pharmacol Ther1992，55，： | 1 |
| 10 | T cell killing does not require the formation of a stable mature immunological synapse 显示文摘 | Purbhoo M A Irvine D J Huppa J B | 2004 | Nature Immunology2004，5，： | 1 |
| 11 | Fire hazards and some common polymers 显示文摘 | Irvine D J McCluskey J A Robinson I M | 2000 | Polymer Degradation and Stability2000，67，： | 1 |
| 12 | Noncoding RNAs and gene silencing显示文摘 | ZARATIEGUI M IRVINE D V MARTIENSSEN R A | 2007 | Cell2007，128，： | 1 |
| 13 | Oxidation of phenolic pollutants by a lignin degrading enzyme from the white-rot fungus Phanerochaete chrysosporium 显示文摘 | Aitken M D Venkatadri R Irvine R L | 1989 | Water Research1989，23，4： | 1 |
| 14 | Fire hazards and some common polymers显示文摘 | IRVINE D J MCCLUSKEY J A ROBINSON I M | 2007 | Polymer Degradation and Stability2007，67，： | 1 |
| 15 | Methods for exploiting the relationship between building and their shadows in aerial imagery 显示文摘 | Irvin R B Mckeown D M | 1989 | IEEE Trans System Man and Cybemetics1989，19，6： | 1 |
| 16 | MOCVD of highly conductive CdO thin films显示文摘 | Ellis D M Irvine S J C | 2004 | Journal of Materials Science: Materials in Electronics2004，15，： | 1 |
| 17 | Fire hazards and some common polymers显示文摘 | Irvine D J Mc Cluskey J A Robinson I M | 2000 | Polymer Degradation and Stability2000，67，3： | 1 |
| 18 | Quantifying signaling-induced reorientationof T cell receptors during immunological synapse formation 显示文摘 | Moss W C Irvine D J Davis M M | 2002 | Proc Natl Acad Sci USA2002，99，15： | 1 |
| 19 | Self-consistent field analysis of grafted star polymers显示文摘 | Irvine D J Mayes A M Cima L G | 1996 | Macromolecules1996，29，： | 1 |
| 20 | Oxidation of phenolic pollutants by a lignin degrading enzyme from the white rot fungus Phanerochaete chrysosporium显示文摘 | AITKEN M D VENDATATRI R IRVINE R | 1989 | Wat Res1989，23，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员