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| 1 | Acute effects of rotavirus and malnutrition on intestinal barrier function in neonatal piglets显示文摘AIM: To investigate the effect of protein-energy malnutrition on intestinal barrier function during rotavirus enteritis in a piglet model.METHODS: Newborn piglets were allotted at day 4 of age to the following treatments:(1) full-strength formula(FSF)/noninfected;(2) FSF/rotavirus infected;(3) half-strength formula(HSF)/noninfected;or(4) HSF/rotavirus infected.After one day of adjustment to the feeding rates,pigs were infected with rotavirus and acute effects on growth and diarrhea were monitored for 3 d and jejunal samples were collected for Ussingchamber analyses.RESULTS: Piglets that were malnourished or infected had lower body weights on days 2 and 3 post-infection(P < 0.05).Three days post-infection,marked diarrhea and weight loss were accompanied by sharp reductions in villus height(59%) and lactase activity(91%) and increased crypt depth(21%) in infected compared with non-infected pigs(P < 0.05).Malnutrition also increased crypt depth(21%) compared to full-fed piglets.Villus:crypt ratio was reduced(67%) with viral infection.There was a trend for reduction in transepithelial electrical resistance with rotavirus infection and malnutrition(P = 0.1).3H-mannitol flux was significantly increased(50%;P < 0.001) in rotavirus-infected piglets compared to non-infected piglets,but there was no effect of nutritional status.Furthermore,rotavirus infection reduced localization of the tight junction protein,occludin,in the cell membrane and increased localization in the cytosol.CONCLUSION: Overall,malnutrition had no additive effects to rotavirus infection on intestinal barrier function at day 3 post-infection in a neonatal piglet model. | Sheila K Jacobi Adam J Moeser Anthony T Blikslager J Marc Rhoads Benjamin A Corl Robert J Harrell Jack Odle | 2013 | World Journal of Gastroenterology2013，19，31： | 4 |
| 2 | The effects of epithelial cell supernatant on contractions of isolated canine tracheal smooth muscle显示文摘 | Barnett K Jacoby D B Nadel J A | 1988 | Am Rev Respir Dis1988，138，4： | 2 |
| 3 | A functional classification scheme for beta-lactamases and its correlation with molecular structure 显示文摘 | Bush K Jacoby G A Medeiros A A | 1995 | Antimicrob Agents Chemother1995，39，： | 2 |
| 4 | Ru(0001)表面上O-Ru伸缩振动的覆盖度依赖特性显示文摘在Ru(0001)表面上,通过氧气以及二氧化氮气体的分解吸附,制备了具有不同氧覆盖度的氧覆盖层.高分辨电子能量损失谱的测量表明,ORu伸缩振动具有强烈的覆盖度依赖特性。 | 何丕模 Jacobi,K | 1999 | 物理学报1999，48，2： | 2 |
| 5 | A functional classification scheme for β-lactamases and its correlation with molecular structure显示文摘 | Bush K Jacoby GA Medeiros AA | 1995 | Antimicrob Agents Chemother1995，39，6： | 1 |
| 6 | A functional classification scheme for β-lactamases and its correlation with molecular structure 显示文摘 | Bush K Jacoby GA Medeiros AA | 1995 | Antimicrob Agents Clemother1995，39，6： | 1 |
| 7 | Assessment of tobacco heating system 2.4 on osteogenic differentiation of mesenchymal stem cells and primary human osteoblasts compared to conventional cigarettes显示文摘BACKGROUND Cigarette smoking(CS)is the most common method of consuming tobacco.Deleterious effects on bone integrity,increased incidence of fractures,and delayed fracture healing are all associated with CS.Over 150 of the 6500 molecular species contained in cigarette smoke and identified as toxic compounds are inhaled by CS and,via the bloodstream,reach the skeletal system.New technologies designed to develop a reduced-risk alternative for smokers are based on electronic nicotine delivery systems,such as e-cigarettes and tobacco heating systems(THS).THS are designed to heat tobacco instead of burning it,thereby reducing the levels of harmful toxic compounds released.AIM To examine the effects of THS on osteoprogenitor cell viability and function compared to conventional CS.METHODS Human immortalized mesenchymal stem cells(n=3)and primary human preosteoblasts isolated from cancellous bone samples from BG Unfall Klinik Tübingen(n=5)were osteogenically differentiated in vitro with aqueous extracts generated from either the THS 2.4“IQOS”or conventional“Marlboro”cigarettes for up to 21 d.Cell viability was analyzed using resazurin conversion assay(mitochondrial activity)and calcein-AM staining(esterase activity).Osteogenic differentiation and bone cell function were evaluated using alkaline phosphatase(AP)activity,while matrix formation was analyzed through alizarin red staining.Primary cilia structure was examined by acetylatedα-tubulin immunofluorescent staining.Free radical production was evaluated with 2’,7’-dichlorofluoresceindiacetate assay.RESULTS Our data clearly show that THS is significantly less toxic to bone cells than CS when analyzed by mitochondrial and esterase activity(P<0.001).No significant differences in cytotoxicity between the diverse flavors of THS were observed.Harmful effects from THS on bone cell function were observed only at very high,non-physiological concentrations.In contrast,extracts from conventional cigarettes significantly reduced the AP activity(by two-fold)and matrix mineralization(four-fold)at low concentrations.Additionally,morphologic analysis of primary cilia revealed no significant changes in the length of the organelle involved in osteogenesis of osteoprogenitor cells,nor in the number of ciliated cells following THS treatment.Assessment of free radical production demonstrated that THS induced significantly less oxidative stress than conventional CS in osteoprogenitor cells.CONCLUSIONTHS was significantly less harmful to osteoprogenitor cells during osteogenesisthan conventional CS. Additional studies are required to confirm whether THS isa better alternative for smokers to improve delays in bone healing followingfracture. | Romina H Aspera-Werz Sabrina Ehnert Monja Müller Sheng Zhu Tao Chen Weidong Weng Johann Jacoby Andreas K Nussler | 2020 | World Journal of Stem Cells2020，12，8： | 1 |
| 8 | A functional classification scheme for β - lactamases and its correlation with molecular structure 显示文摘 | Bush K Jacoby GA Medeiros AA | 1995 | Antimicrob Agents Chemother1995，39，： | 1 |
| 9 | A functional classification scheme for β-lactamases and its correlation with molecular structure 显示文摘 | Bush K Jacoby GA Medeiros AA | 1995 | Antimicrob Agents Chemother1995，39，6： | 1 |
| 10 | Tackling antibiotic resistance显示文摘 | Bush K Courvalin P Dantas G Davies J Eisenstein B Huovinen P Jacoby GA Kishony R Kreiswirth BN Kutter E Lerner SA Levy S Lewis K Lomovskaya O Miller JH Mobashery S Piddock LJ Projan S Thomas CM Tomasz A Tulkens PM Walsh TR Watson JD Witkowski J Witte W Wr | | 0，，： | 1 |
| 11 | Chemopreventive efficacy of combined piroxicam and difluoromethylornithine treatment of Apc mutant Min mouse adenomas,and selective toxicity against Apc mutant embryos显示文摘 | Jacoby RF Cole CE Tutsch K | 2000 | Cancer Res2000，60，7： | 1 |
| 12 | A functional classification scheme for β-lactam and its correlation with molecular structure显示文摘 | 5,Bush K Jacoby GA Medeiros AA | 1995 | Antimicrob Agents Chemother1995，39，： | 1 |
| 13 | A functional classification scheme for beta lactamases and its correlation with molecular structure显示文摘 | Bush K Jacoby GA Medeiros AA | 1995 | Antimicrob Agent Chemother1995，39，： | 1 |
| 14 | Chemopreventive efficacy of combiped piroxieam and flu orome thylomithine treatment of Ape mutant Min mouse adenomas and selective toxicity against Ape mutant embryos显示文摘 | Jacoby RF Cole CE Tutsch K | 2000 | Cancer Res2000，60，： | 1 |
| 15 | Hydrogen vibrations on the Ru(001) surface revisited显示文摘 | Shi H Jacobi K | 1994 | Surface Science1994，313，： | 1 |
| 16 | Air-side flow and heat transfer in compact heat exchangers: a discussion of enhancement mechanisms显示文摘 | Jacobi A M Shah R K | 1998 | Heat Transfer Engineering1998，19，4： | 1 |
| 17 | Molecular analysis of the NF2 tumor-suppressor gene in schwannomatosis显示文摘 | Jacoby L B Jones D Davis K Kronn D Short M P Gusella J | 1997 | Am J Hum Genet1997，61，： | 1 |
| 18 | NF2 gene analysis distin-guishes hemangiopercytoma from meningioma显示文摘 | Joseph J T Lisle D K Jacoby L B | 1995 | Am J Pathol1995，147，5： | 1 |
| 19 | Moderate controlled hypotension with sodium nitroprusside does not improve surgical conditions or decrease blood loss in endoscopic sinus surgery显示文摘 | Jacobi K E Boehm B E Rickauer A G | 2000 | J Clin Anesth2000，12，3： | 1 |
| 20 | Updated functional classification of betalacta- mases显示文摘 | Bush K Jacoby GA | 2010 | Antimicrob Agents Chemother2010，54，3： | 1 |
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