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| 1 | Label-free visualization of cholesteatoma in the mastoid and tympanic membrane using CARS microscopy显示文摘Objective:The present study aimed to evaluate the possibility of using coherent anti-Stokes Raman spectroscopy(CARS) microscopy to determine the specific molecular morphology of cholesteatoma by detecting the natural vibrational contrast of the chemical bonds without any staining.Materials and methods:Specimens from the mastoid and tympanic membrane with and without cholesteatoma were analyzed using CARS microscopy,two-photon excited fluorescence(TPEF) microscopy,and the second harmonic generation(SHG) microscopy.Results:In cholesteatoma tissues from the mastoid,a strong resonant signal at 2845 cm^(-1) was observed by CARS,which indicated the detection of the CH_2 hydro-carbon lipid bonds that do not generate visible signals at 2940 cm^(-1) suggestive of CH_3 bonds in amino acids.A strong resonant signal at 2940 cm^(-1) appeared in an area of the same specimen,which also generated abundant signals by TPEF and SHG microscopy at 817 nm,which was suggestive of collagen.In the tympanic membrane specimen with cholesteatoma,a strong resonant signal with corrugated morphology was detected,which indicated the presence of lipids.A strong signal was detected in the tympanic membrane with chronic otitis media using TPEF/SHG at 817 nm,which indicated collagen enrichment.The CARS and TPEF/SHG images were in accordance with the histology results.Conclusion:These results suggest the need to develop a novel CARS microendoscope that can be used in combination with TPEF/SHG to distinguish cholesteatoma from inflammatory tissues. | Jing Zou Antti Isomaki Timo Hirvonen Antti Aarnisalo Jussi Jero Ilmari Pyykko | 2016 | Journal of Otology2016，11，3： | 4 |
| 2 | Human adipose-derived mesenchymal stem cells accelerate decellularized neobladder regeneration显示文摘Decellularized natural bladder matrices(neobladders)represent an exciting means to regenerate the bladder following bladder cancer-associated cystectomy.In this study,we compare the evolution of decellularized matrices with recellularized matrices by seeding it with human adiposederived mesenchymal stem cells(ADSC)after implantation following partial cystectomy in rats.We discovered significant anatomical differences since 10 days after neobladder implantation with the ADSC-containing matrices promoting a significant recovery of mature p63-and cytokeratin 7-positive urothelium.We also discovered significantly induced expression of the vimentin mesoderm marker in the submucosal layer in ADSC-seeded matrices.Interestingly,we found a higher expression of smooth muscle actin in transversal and longitudinal smooth muscle layers with ADSC-seeded matrices.Furthermore,ADSC also showed increased vascularization and nerve innervation of the neobladder as determined by the distribution of CD31 and S100b reactivity,respectively.We believe that ADSC and their paracrine-acting pro-regenerative secretome within decellularized matrices represent an efficient bladder substitution strategy;however,we require a fuller understanding of the mechanisms involved before clinical studies can begin. | Victoria Moreno-Manzano Maravillas Mellado-Lo´pez Maria Jose Morera-Esteve Ana Alastrue-Agudo Viviana Bisbal-Velasco Jero´nimo Forteza-Vila A´ngel Serrano-Aroca Ce´sar David Vera-Donoso | 2020 | Regenerative Biomaterials2020，7，2： | 2 |
| 3 | Aquaporin-2 expression in the mammalian cochlea and investigation of its role in Meniere's disease显示文摘 | Mhatre AN Jero J Chiappini I | 2002 | Hear Res2002，170，： | 1 |
| 4 | New method for coating tympanostomy tubes to prevent tube occlusions 显示文摘 | KINNARI T J SALONEN E M JERO J | 2001 | Int J Pediatr Otorhinolaryngol2001，58，： | 1 |
| 5 | 芬兰人工耳蜗植入术加强无菌措施的方法显示文摘人工耳蜗植入已经在全球广泛开展并帮助广大聋病患者重新返回正常生活,且并发症的发生率也越来越少;然而,围手术期和术后晚期感染性并发症仍然时有发生。在某些特殊病例中,当抗生素无效时,植入体也将会被废弃,重新植入将会显著增加患者经济负担。芬兰的所有人工耳蜗植入中心都采用一种术中加强无菌措施以便预防或者减少感染性并发症的发生。其关键点是,从手术室的术前准备到完成植入手术,全程严格实施无菌操作,在完成颞骨钻磨工作以后,清理术野,医生和护士重新进行手部消毒,更新术区的无菌巾和医护人员的手套,打开人工耳蜗植入体的包装,固定植入体并安装耳蜗电极,完成后续程序。在仅预防性使用抗生素的情况下,610次人工耳蜗植入未发生植入体的原发性感染并发症。邹静于2016年在赫尔辛基大学中心医院耳鼻咽喉头颈外科学习人工耳蜗植入的临床相关技术,将这一加强无菌措施在长海医院推广应用。除了因为医院的实际情况而存在一些较小的差异外,关键步骤完全可以在长海医院实施。本文将对该技术进行详细报道,期望今后在中国全国推广这一效价比很高的技术。 | 邹静 秦晶 姜晓瑜 李勇 周蓉珏 徐菲 Timo Hirvonen Antti Aarnisalo Jussi Jero 郑宏良 | 2018 | 中华耳科学杂志2018，16，1： | 1 |
| 6 | Aquaporiin-2 expression inthe mammalian cochlea and investigation of its role in Meniere'sdisease显示文摘 | Mhatre AN Jero J Chiappini I | 2002 | Hear Res2002，170，12： | 1 |
| 7 | Polymerase chain reactionbased detection of rhinovirus,respiratory syncytial virus,and coronavirus in otitis media with effusion显示文摘 | Pitk(a)ranta A Jero J Arruda E | 1998 | J Pediatr1998，133，3： | 1 |
| 8 | Calci- fication reduction and recovery in native and non-native Mediterranean corals in response to ocean acidification 显示文摘 | MOVILLA J CALVO E PEL | 2012 | Journal of Experimental Marine Biology and Ecol- ogy2012，438，4： | 1 |
| 9 | Current issues in cochlear gene transfer显示文摘 | Lalwani AK Jero J Mhatre AN | 2002 | Audiol Neurootol2002，7，3： | 1 |
| 10 | The bactericidal/permeability-increasing protein (BPI) is present in specific granules of human e osinophils显示文摘 | Jero C Hans J Anton T | 1998 | Blood1998，91，： | 1 |
| 11 | Mastoid bio- film in chronic otitis media显示文摘 | Lampikoski H Aarnisalo AA Jero J | 2012 | Otol Neurotol2012，33，5： | 1 |
| 12 | Gap junction protein connexin-43 interacts directly with microtubules显示文摘 | Ben N.G. Giepmans Ingrid Verlaan Trudi Hengeveld Hans Janssen Jero Calafat Matthias M. Falk Wouter H. Moolenaar | 2001 | Current Biology2001，，17： | 1 |
| 13 | Cochlear gene delivery through an intact round window membrane in mouse显示文摘 | Jero J Mhatre AN Tseng CJ | 2001 | Hum Gene Ther2001，12，： | 1 |
| 14 | Aquaporin -2 expres- sionin the mammalian cochlea and investigation of its role in Me- niere 'sdisease显示文摘 | Mhatre AN Jero J Chiappini I | 2002 | Hear Res2002，170，： | 1 |
| 15 | Aquaporin-2 expression in the mammalian cochlea and investigation of its role in Menieres disease显示文摘 | Mhatre AN Jero J Chiappini I | 2002 | Hearing Res2002，170，12： | 1 |
| 16 | Oral predni- solone is an effective adjuvant therapy for acute otitis media with discharge through tympanostomy tubes 显示文摘 | RUOHOLA A HEIKKINEN T JERO J et ol | 1999 | J Pediatr1999，134，4： | 1 |
| 17 | Cochlear gene delivery through an intact round window membrane in mouse显示文摘 | Jero J Mhatre AN Tseng CJ | 2001 | Human Gene Therapy2001，12，： | 1 |
| 18 | Current issues in cochlear gene transfer显示文摘 | Lalwani AK Jero J Mhatre AN | 2002 | Audiol Neurootol2002，7，3： | 1 |
| 19 | Cochlea gene delivery through an intact round window membrane in mouse 显示文摘 | Jero J Mhatre AN Tseng CJ | 2001 | Hum Gene Ther2001，12，5： | 1 |
| 20 | Post auxiliary dissection pain in breast cancer due to lesion of the intercostobrach ial never显示文摘 | Vetch C J Vander BrandH J Wa jerO JM | | 0，，2： | 1 |
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