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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | Fuzzy-decisionmaking problems of fuel ethanol production using a genetically engineered yeast显示文摘 | Wang F S Jing C H Tsao G T | 1998 | Industrial & Engineering Chemistry Research1998，37，8： | 1 |
| 3 | Adaptive tracking control for dynamic nonholonomic mobile robots with uncalibrated visual parameters 显示文摘 | YANG F WANG C L JING G Z | 2013 | International Journal of Adaptive Control and Signal Processing2013，27，8： | 1 |
| 4 | Atomic oxygen diffusion on and desorption from amorphous silicate surfaces显示文摘 | He Jiao Jing Dapeng Vidali G F | 2014 | Chem Phys2014，16，1： | 1 |
| 5 | Tough polylactide graft copolymers显示文摘 | THERYO G JING F PITET L M | 2010 | Macromolecules2010，43，18： | 1 |
| 6 | Fuzzy-decision-making problems of fuel ethanol production using a genetically engineered yeast显示文摘 | WANG F S JING C H TSAO G T | 1998 | Industrial & Engineering Chemistry Research1998，37，8： | 1 |
| 7 | High-speed jet electrodeposition and microstructure of nanocrystalline Ni-Co alloys 显示文摘 | Qiao G Y Jing T F Wang N | 2005 | Electrochimica Acta2005，51，1： | 1 |
| 8 | Time-resolved dynamic tensile spallation of pure aluminum under laser irradiation显示文摘 | Wang Y G He H L Wang L L Jing F Q Boustie M Sekine T | 2006 | Journal of Applied Physics2006，100，03： | 1 |
| 9 | Oxidation of oily sludge in supercritical water显示文摘 | CUI B C CUI F Y JING G L XU S L HUO WJ LIU S Z | 2009 | J Hazard Mater2009，165，13： | 1 |
| 10 | Priori-ty- Based Traffic Scheduling and Utility Optimization for Cognitive Radio Communication Infrastructure-Based Smart Grid 显示文摘 | JING F H HONG G W YI Q A | 2013 | IEEE Transactions on Smart Grid2013，4，1： | 1 |
| 11 | Lipid peroxidation,stellate cell activation and hepatic fibrogenesis in a rat model of chronic steatohepatitis显示文摘 | Jacob G Natasha P Nghi P Isabelle L Jing YH Geoffrey F | 2003 | J Hepatol2003，39，： | 1 |
| 12 | Dynamic Ductile Fragmentation and the Damage Function Model显示文摘 | Feng J B Jing F Q Zhang G R | 1997 | J Appl Phys1997，81，： | 1 |
| 13 | Rhein affects arylamine N-acetyltransferase activity in helicobacter pylori from peptic ulcer patients显示文摘 | Jing G Chung Mei F Tsou Hwang H Wang | 1998 | J Appl Toxicol1998，2，18： | 1 |
| 14 | Theoretical study on structures and energetics of Ge2P2显示文摘 | HAO F Y HAO Y F JING X G | 2006 | J Mol Struct (Theochem)2006，764，： | 1 |
| 15 | Nitrogen budget of a tipical subterranean riv er in peak cluster karst area显示文摘 | Gou F Jing G H | 2009 | Environmental Geology2009，58，8： | 1 |
| 16 | Fuzzy-decision-making problems of fuel ethanol production using a genetically engineered yeast显示文摘 | Wang F S Jing C H Tsao G T | 1998 | Industrial & Engineering Chemistry Research1998，37，8： | 1 |
| 17 | Dynamic ductile fragmentation and the damage function model显示文摘 | Feng J B Jing F Q Zhang G R | 1997 | J Appl Phys1997，81，6： | 1 |
| 18 | High-speed jet electrodeposition and microstructure of nanocrystalline Ni-Co alloys显示文摘 | Qiao G Y Jing T F Wang N | 2005 | Electrochimica Acta2005，51，1： | 1 |
| 19 | A new family of dendtimers with naphtha-line core and triphenylamine branching as a two-photon polymerization initiator 显示文摘 | Mang X M Jing F Chen Z G et al | 2011 | J Phys Chem C2011，115，： | 1 |
| 20 | The characteristics of hydrolysis of triolein catalyzed by wheat germ lipase in water-in-oil microemulsions 显示文摘 | JING F AN X Q SHEN W G | 2003 | Journal of Molecular Catalysis B: Enzymatic2003，2425，： | 1 |
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