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| 1 | Ghrelin attenuates gastrointestinal epithelial damage induced by doxorubicin显示文摘AIM:To examine the influence of ghrelin on the regenerative potential of gastrointestinal(GI)epithelium.METHODS:Damage to GI epithelium was induced in mice by two intravenous injections of doxorubicin(10 and 6 mg/kg).Some of the doxorubicin-treated mice received a continuous subcutaneous infusion of ghrelin(1.25μg/h)for 10 d via implanted mini-osmotic pumps.To label dividing stem cells in the S-phase of the cell cycle,all mice received a single intraperitoneal injection of 5'-bromo-2'-deoxyuridine(BrdU)one hour before sacrifice.The stomach along with the duodenum were then removed and processed for histological examination and immunohistochemistry using anti-BrdU antibody.RESULTS:The results showed dramatic damage to the GI epithelium 3 d after administration of chemotherapy which began to recover by day 10.In ghrelintreated mice,attenuation of GI mucosal damage was evident in the tissues examined postchemotherapy.Immunohistochemical analysis showed an increase in the number of BrdUlabeled cells and an alteration in their distribution along the epithelial lining in response to damage by doxorubicin.In mice treated with both doxorubicin and ghrelin,the number of BrdUlabeled cells was reduced when compared with mice treated with doxorubicin alone.CONCLUSION:The present study suggests that ghrelin enhances the regenerative potential of the GI epithelium in doxorubicintreated mice,at least in part,by modulating cell proliferation. | Mohamed A Fahim Hazem Kataya Rkia El-Kharrag Dena AM Amer Basel al-Ramadi Sherif M Karam | 2011 | World Journal of Gastroenterology2011，17，33： | 3 |
| 2 | Clinical benefits of biochemical markers of bone turnover in Egyptian children with chronic liver diseases显示文摘AIM: To investigate the association between serum insulin-like growth factor 1 (IGF-1), osteocalcin, and parathyroid hormone (PTH) levels with the etiology and clinical condition of patients with chronic liver disease.METHODS: Eighty children with hepatocellular damage were divided into 3 groups according to the etiology of disease infection: bilharziasis (9 patients), hepatitis B virus (HBV, 12 patients) and hepatitis C virus (HCV, 29 patients). The Child score index was found as A in 24 patients, B in 22 patients, C in 4 patients. Thirty healthy children served as control group. HBsAg, HBcAbIgM, HBcAbIgG, and anti-HCV were detected using ELISA technique. HCV-RNA was measured by reverse transcription polymerase chain reaction (RT-PCR). Anti-bilharzial antibodies were detected by indirect haem- agglutination test. Liver function tests were performed using autoanalyser. Serum IGF-1, osteocalcin and PTH levels were measured by ELISA technique. Abdominal ultrasonography was also conducted.RESULTS: Serum IGF-1 level was significantly lower in all patient groups with liver diseases, while serum osteocalcin and PTH levels were significantly elevated in patients with HBV and HCV infections compared with the control group. Serum osteocalcin and PTH concentrations were measured with the severity of liver disease from Child A to C. Child A patients unexpectedly showed significantly reduced IGF-1 levels in comparison to patients staged as Child B or C. Serum osteocalcin level was negatively correlated with albumin (14.7 ± 0.54 vs 3.6 ± 0.10, P < 0.05), while that for PTH was positivelycorrelated with total protein (70.1 ± 2.17 vs 6.7 ± 0.10, P < 0.05) in patients with HCV infection.CONCLUSION: Low serum IGF-1 level seems to play a critical role in the bone loss in patients with chronic liver disease. Elevated biochemical markers of bone remodeling suggest high-turnover in patients with viral infection and reflect severity of the clinical stage. | Karam A Mahdy Hanaa H Ahmed Fathia Mannaa Azza Abdel-Shaheed | 2007 | World Journal of Gastroenterology2007，13，5： | 3 |
| 3 | Profiling cellular bioenergetics, glutathione levels, and caspase activities in stomach biopsies of patients with upper gastrointestinal symptoms显示文摘AIM: To measure biochemical parameters in stomach biopsies and test their suitability as diagnostic biomarkers for gastritis and precancerous lesions.METHODS: Biopsies were obtained from the stomachs of two groups of patients(n = 40) undergoing fiberoptic endoscopy due to upper gastrointestinal symptoms. In the first group(n = 17), only the corpus region was examined. Biopsies were processed for microscopic examination and measurement of mitochondrial O2 consumption(cellular respiration), cellular adenosine triphosphate(ATP), glutathione(GSH), and caspase activity. In the second group of patients(n = 23), both corpus and antral regions were studied. Some biopsies were processed for microscopic examination, while the others were used for measurements of cellular respiration and GSH level.RESULTS: Microscopic examinations of gastric corpus biopsies from 17 patients revealed normal mucosae in 8 patients, superficial gastritis in 7 patients, and chronic atrophic gastritis in 1 patient. In patients with normal histology, the rate(mean ± SD) of cellular respiration was 0.17 ± 0.02 μmol/L O2 min-1 mg-1, ATP content was 487 ± 493 pmol/mg, and GSH was 469 ± 98 pmol/mg. Caspase activity was detected in 3 out of 8 specimens. The values of ATP and caspase activity were highly variable. The presence of superficial gastritis had insignificant effects on the measured biomarkers. In the patient with atrophic gastritis, cellular respiration was high andATP was relatively low, suggesting uncoupling oxidative phosphorylation. In the second cohort of patients, the examined biopsies showed either normal or superficial gastritis. The rate of cellular respiration(O2. μmol/L min-1 mg-1) was slightly higher in the corpus than the antrum(0.18 ± 0.05 vs 0.15 ± 0.04, P = 0.019). The value of GSH was about the same in both tissues(310 ± 135 vs 322 ± 155, P = 0.692).CONCLUSION: The corpus mucosa was metabolically more active than the antrum tissue. The data in this study will help in understanding the pathophysiology of gastric mucosa. | Ali S Alfazari Bayan Al-Dabbagh Wafa Al-Dhaheri Mazen S Taha Ahmad A Chebli Eva M Fontagnier Zaher Koutoubi Jose Kochiyi Sherif M Karam Abdul-Kader Souid | 2015 | World Journal of Gastroenterology2015，21，2： | 2 |
| 4 | Inflammatory bowel disease in rats:Bacterial and chemical interaction显示文摘AIM: To develop a novel model of colitis in rats, using a combination of iodoacetamide and enteropathogenic E. coli (EPEC), and to elucidate the pathophysiologic processes implicated in the development of ulcerative colitis (UC). METHODS: Male Sprague-Dawley rats (n = 158) were inoculated intrarectally on a weekly basis with 4 different combinations: (a) 1% methylcellulose (MC), (b) 100 μL of 6% iodoacetamide (IA) in 1% MC, (c) 200 μL containing 4 × 108 colony factor units (CFU) of EPEC, and (d) combined treatment of (IA) followed by bacteria (B) after 2 d. Thirty days post treatment, each of the four groups was divided into two subgroups; the inoculation was stopped for one subgroup and the other subgroup continued with biweekly inoculation until the end of the experiment. Colitis was evaluated by the clinical course of the disease, the macroscopic and microscopic alterations, activity of myeloperoxidase (MPO), and by TNF-α gene expression. RESULTS: Findings indicative of UC were seen in the combined treatment (IA + B) as well as the IA conti-nued treatment groups: the animals showed slow rate of increase in body weight, diarrhea, bloody stools, high colonic ulcer score, as well as histological altera- tions characteristic of UC, with an extensive inflamma- tory reaction. During the course of the experiment, the MPO activity was consistently elevated and the TNF-α gene expression was upregulated compared to the control animals. CONCLUSION: The experimental ulcerative colitis model used in the present study resembles, to a great extent, the human disease. It is reproducible with characteristics indicative of chronicity. | Inaya Abdallah Hajj Hussein Rania Tohme Kassem Barada Mostafa Hassan Mostafa Jean-Noel Freund Rosalyn A Jurjus Walid Karam Abdo Jurjus | 2008 | World Journal of Gastroenterology2008，14，25： | 2 |
| 5 | Potato response to crop sequence and nitrogen fertilization following sod breakup in a Gleyed Humo- Ferric Podzol显示文摘 | Li H L I~ Parent C T Karam A | 2009 | Can J Plant Sci2009，79，： | 1 |
| 6 | Finite element analysis of a coupled thermally dependent viscosity flow problem 显示文摘 | Zhu J Loula A F D Karam F J Guerreiro J N C | 2007 | Comput Appl Math2007，26，1： | 1 |
| 7 | Elastin expression and elastic fibre width in the anterior vaginal wall of postmenopausal women with and without prolapse显示文摘 | Karam J A Vazquez D V Lin V K | 2007 | BJU Int2007，100，2： | 1 |
| 8 | Evidence for an association of human papillomavirus and breast carcinomas显示文摘 | Damin A P Karam R Zettler C G | 2004 | Breast Cancer Res Treat2004，84，2： | 1 |
| 9 | A Microwave Polarimetric Scattering Model for Forest Canopies Based on Vector Radiative Transfer Theory显示文摘 | Karam M A Amar F Fung A K | 1995 | Remote Sens Environ1995，53，1： | 1 |
| 10 | Ovarian cancer:the duplicity of CA125 measurement显示文摘 | Karam A K Karlan B Y | 2010 | Nat Rev Clin Oncol2010，7，6： | 1 |
| 11 | Cryopreservation of Sour Orange (Citrus aurantium) Shoot Tips 显示文摘 | Al-ABABNEH S S KARAM N S SHIBLI R A | 2002 | In Vitro Cellular and Developmental Biology-Plant2002，38，6： | 1 |
| 12 | The precise location and nature of the nerves to the male human urethra: histological and immunohistochemical studies with three-di- mensional reconstruction显示文摘 | Karam I Droupy S Abd-Alsamad I Korbage A Uhl JF Benoit G | 2005 | Eur Urol2005，48，： | 1 |
| 13 | Atmo- spheric Surface Layer Characteristics of Turbulence above the Pantanal Wetland Regarding the Similarity Theory显示文摘 | MARQUES F E P S&L D A KARAM H A | 2008 | Agricultural and Forest Meteorology2008，148，67： | 1 |
| 14 | Zygomycosis caused by Cunninghamella bertholletiae in a kidney transplant recipient显示文摘 | Quinio D Karam A Leroy JP | 2004 | Med Mycol2004，42，： | 1 |
| 15 | Ovarian cancer: the duplicity of CA125 measurement 显示文摘 | Karam A K Karlan B Y | 2010 | Nat Rev Clin Oneol2010，7，6： | 1 |
| 16 | Management of type 2diabetes mellitus in the elderly显示文摘 | Soe K Sacerdote A Karam] et aZ | 2011 | Maturitas2011，70，2： | 1 |
| 17 | Atmospheric surface layer characteristics of turbulence above the Pantanal wetland regarding the similarity theory显示文摘 | Marques Filho E P Sa L D A Karam H A | 2008 | Agricultural and Forest Meteorology2008，148，67： | 1 |
| 18 | Effect of salinity on water stress, growth, and yield of maize and sunflower显示文摘 | Katerji N van Hoorn J W Hamdy A Karam F Mastrorilli M | 1996 | Agricultural Water Management1996，30，： | 1 |
| 19 | Neoadjuvant cisplatin andradical cesarean hysterectomy for cervical cancer in pregnancy 显示文摘 | Karam A Feldman N Holschneider CH | 2007 | Nat Clin Pract Oncol2007，4，6： | 1 |
| 20 | A modified version of CERES-Maize model for predicting crop response to salinity stress 显示文摘 | Castrignano A Kajerti N Karam F | 1998 | Ecological Modelling1998，111，： | 1 |
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