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| 1 | Differential roles of EPS8 in carcinogenesis:Loss of protein expression in a subset of colorectal carcinoma and adenoma显示文摘AIM:To analyze the epidermal growth factor receptor pathway substrate 8(EPS8) expression status and role in colorectal carcinogenesis given that EPS8 has a conserved actin barbed-end capping function that is required for proper maturation in intestinal cells.METHODS:We studied 8 colon cancer cell lines and 58 colorectal tumors(19 adenomas and 39 carcinomas).We performed expression microarray analysis of colon cancer cell lines followed by loss of heterozygosity(LOH)analysis and immunohistochemistry for EPS8 expression in colon tumors.Subsequently,we performed mutation analysis by direct sequencing and methylation analysis by bisulfite sequencing and methylation-specific polymerase chain reaction assays.RESULTS:Expression microarray analysis of colon cancer cell lines showed overexpression of EPS8 transcript in all lines but RKO.Genome wide loss of heterozygosity(LOH) analysis of colon tumors,showed considerable LOH at the EPS8 gene locus.Immunohistochemically,EPS8 was constitutively expressed in normal colonic mucosa with a dot-like supranuclear localization with accentuation at the luminal surface supporting its proposed role in epithelial maturation.Nineteen colon tumors(4 adenoma,15 carcinoma) out of 51(37%) showed strikingly tumor specific EPS8 protein loss.Of the remaining tumors,5/51(2 adenoma,and 3 carcinoma,10%) showed marked overexpression,while 27/51 tumors(53%) showed retained expression.Mutation analysis revealed a missense mutation(c.794C>T,p.R265C) in exon 8 in RKO.The EPS8 promoter was also methylated in RKO,but there was no significant methylation in other cell lines or carcinoma specimens.CONCLUSION:The loss of EPS8 expression in colorectal adenomas and carcinomas suggests that down regulation of this gene contributes to the development of a subset of colorectal cancers,a finding which could have applications in diagnosis and treatment. | Wael M Abdel-Rahman Salla Ruosaari Sakari Knuutila Pivi Peltomki | 2012 | World Journal of Gastroenterology2012，18，29： | 5 |
| 2 | Solution structure of the epithelial cadherin domain responsible for selective cell adhesion显示文摘 | Overduin M Harvey TS Bagby S Tong KI Yau P Takeichi M Ikura M | | 0，，： | 2 |
| 3 | The association of metabolic syndrome with adipose tissue hormones and insulin resistance in patients with COPD without co- morbidities显示文摘 | Minas M Kostikas K Papaioannou AI Mystridou P Karetsi E Georgoulias P Liakos N Pournaras S Gourgoulianis KI | 2011 | COPD2011，8，6： | 1 |
| 4 | Determination of platinum and rhodium in dust and plant samples using microwave-assisted sample digestion and ICP-MS显示文摘 | NIEMEL魧M PER魧M魧KI P PIISPANEN J | 2004 | Analytica Chimica Acta2004，，521： | 1 |
| 5 | Elevated cystatin C concentration and pro- gression to prediabetes: The Western New York Study显示文摘 | Donahue R P Stranges S Rejman K Rafalson L B Dmochows- ki J Trevisan M | 2007 | Diabetes Care2007，30，： | 1 |
| 6 | Hydrolytic stability of nucleoside phosphotriesters derived from bis(hydroxymethyl) -1,3-dicarbonyl compounds and their congeners:towards a novel pro-drug strategy for antisense oligonucleotides显示文摘 | Esa Mki Pivi Poijrvi | 2001 | J Chem Soc:Perkin Trans 22001，，： | 1 |
| 7 | Diet and breast cancer: evidence that extremes in diet are associated with poor survival 显示文摘 | GOODWIN P J ENNIS M PRITCHARD KI | 2003 | J Clin Oncol2003，21，13： | 1 |
| 8 | A simplified subspace fitting method for estimating shape of a towed array显示文摘 | Hee Y P Ki M K Hyun W K | 2008 | IEEE J Ocean Eng2008，33，2： | 1 |
| 9 | Abnormal myocardial presynaptic norepinephrine recycling in patients with Brugada syndrome显示文摘 | Kies P Wichler T Schafers M | 2004 | Circulation2004，110，19： | 1 |
| 10 | Electrospinning of polyamides with different chain compositions for filtration application 显示文摘 | Heikkila P Taipale A Lehtim? ki M | 2008 | Polymer Engineering & Science2008，48，6： | 1 |
| 11 | Inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia is caused by mutant valosin-containing protein显示文摘 | Watts G D Wymer J Kovach M J Mehta S G Mumm S Darvish D Pestronk A Whyte M P Ki | | 0，，04： | 1 |
| 12 | Glucose transporters regulation on ischemia brain: possiblerole as therapeutic target 显示文摘 | Espinoza-Rojo M Iturralde - Rodriguez KI Aguilera P | 2010 | Cent Nerv SystAgents Med Chem2010，10，4： | 1 |
| 13 | Prolonged exposure of T cells to TNF down-regulates TCR zeta and expression of the TCR/CD3complex at the cell surface显示文摘 | Isomki P Panesar M Annenkov A Clark JM Foxwell BM Chernajovsky Y Cope AP | 2001 | J Immunol2001，166，9： | 1 |
| 14 | Modelling the risk of snowdamage to forests under short-term snow loading显示文摘 | PTALO M L PELTOLA H KELLOM KI S | 1999 | Forest Ecology andManagement1999，116，13： | 1 |
| 15 | Insulin resistance and lipid levels in adolescents with familial risk for psychosis显示文摘 | Koponen H Mki P Halonen H | 2008 | Acta Psychiatr Scand2008，117，5： | 1 |
| 16 | Arrhythmogenic right ventricular dysplasia/cardiomyopathy:screening,diagnosis,and treatment显示文摘 | Kies P Bootsma M Bax J | 2006 | Heart Rhythm2006，3，2： | 1 |
| 17 | Urinary trypsinogen-2 test strip for acute pancreatitis显示文摘 | J Hedst?m U-H Stenman A Korvuo P Kenkim?ki S Tikanoja R Haapiainen E Kivilaakso | 1996 | The Lancet1996，，9003： | 1 |
| 18 | Influence of surface topography on friction characteristics in wet clutch applications显示文摘 | Rikard M(a)ki a Richard Olsson | 2006 | Wear2006，261，： | 1 |
| 19 | Divergent roles of IL-23 and IL-12 in host defense against Klebsiella pneumoniae 显示文摘 | Happel KI Dubin P J Zheng M | 2005 | J Exp Med2005，202，6： | 1 |
| 20 | Identification of successful catheter ablation sites in patients with ventricular tachycardia based on electrogram characteristics during sinus rhythm显示文摘 | Zeppenfeld K Kies P Wijffels M C | 2005 | Heart Rhythm2005，2，9： | 1 |
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