|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 变性梯度凝胶电泳(DGGE)在微生物生态学中的应用显示文摘由于从环境样品中分离和培养细菌的困难 ,分子生物学方法已发展用来描述和鉴定微生物群落。近年来基于 DNA方法的群落分析得到了迅速的发展 ,如 PCR扩增技术 ,克隆文库法 ,荧光原位杂交法 ,限制性酶切片段长度多态性法 ,变性和温度梯度凝胶电泳法。DGGE已广泛用于分析自然环境中细菌、蓝细菌 ,古菌、微微型真核生物、真核生物和病毒群落的生物多样性。这一技术能够提供群落中优势种类信息和同时分析多个样品。具有可重复和容易操作等特点 ,适合于调查种群的时空变化 ,并且可通过对切下的带进行序列分析或与特异性探针杂交分析鉴定群落成员。DGGE分析微生物群落的一般步骤如下 :一是核酸的提取 ,二是 1 6Sr RNA,1 8S r RNA或功能基因如可容性甲烷加单氧酶羟化酶基因 ( mmo X)和氨加单氧酶 α-亚单位基因 ( amo A)片段的扩增 ,三是通过DGGE分析 PCR产物。DGGE使用具有化学变性剂梯度的聚丙烯酰胺凝胶 ,该凝胶能够有区别的解链PCR扩增产物。由 PCR产生的不同的 DNA片段长度相同但核苷酸序列不同。因此不同的双链 DNA片段由于沿着化学梯度的不同解链行为将在凝胶的不同位置上停止迁移 [5] 。 DNA解链行为的不同导致一个凝胶带图案 ,该图案是微生物群落中主要种类的一个轮廓。 DGGE使用所有生物中保守的基因? | 马悦欣 Carola Holmstrm Jeremy Webb Staffan Kjelleberg | 2003 | 生态学报2003，23，8： | 100 |
| 2 | Characterization of the archaeal community fouling a membrane bioreactor显示文摘Biofilm formation, one of the primary causes of biofouling, results in reduced membrane flux or increased transmembrane pressure and thus represents a major impediment to the wider implementation of membrane bioreactor(MBR) technologies for water purification. Most studies have focused on the role of bacteria in membrane fouling as they are the most dominant and best studied organisms present in the MBR. In contrast, there is limited information on the role of the archaeal community in biofilm formation in MBRs. This study investigated the composition of the archaeal community during the process of biofouling in an MBR. The archaeal community was observed to have lower richness and diversity in the biofilm than the sludge during the establishment of biofilms at low transmembrane pressure(TMP). Clustering of the communities based on the Bray–Curtis similarity matrix indicated that a subset of the sludge archaeal community formed the initial biofilms. The archaeal community in the biofilm was mainly composed of Thermoprotei, Thermoplasmata,Thermococci, Methanopyri, Methanomicrobia and Halobacteria. Among them, the Thermoprotei and Thermoplasmata were present at higher relative proportions in the biofilms than they were in the sludge. Additionally, the Thermoprotei, Thermoplasmata and Thermococci were the dominant organisms detected in the initial biofilms at low TMP, while as the TMP increased, the Methanopyri, Methanomicrobia, Aciduliprofundum and Halobacteria were present at higher abundances in the biofilms at high TMP. | Jinxue Luo Jinsong Zhang Xiaohui Tan Diane McDougald Guoqiang Zhuang Anthony G.Fane Staffan Kjelleberg Yehuda Cohen Scott A.Rice | 2015 | Journal of Environmental Sciences2015，27，3： | 2 |
| 3 | Purification and characterization of a novel antibacterial protein from the marine bacterium D2显示文摘 | JAMES S HOLMSTRM C KJELLEBERG S | 1996 | Applied and Environmental Microbiology1996，62，8： | 1 |
| 4 | Chemical defenses of seaweeds against microbial colonization显示文摘 | Steinberg PD Schneider R & Kjelleberg S | 1997 | Biodegradation1997，8，： | 1 |
| 5 | Off the hook-how bacteriasurvive protozoan grazing显示文摘 | Matz C Kjelleberg S | 2005 | Trends in Microbiolo-gy2005，13，7： | 1 |
| 6 | Inhibition of settlement by larvae of Balanus amphitrite and Cliona intestinalis by surface-colonizing marine bacterium 显示文摘 | HOLMSTROM C RITTSCHOF D KJELLEBERG S | 1992 | Applied and environmental Microbiology1992，58，7： | 1 |
| 7 | Growth of Vibrio anguillamm in salmon intestinal mucus显示文摘 | Garcit T Otto K Kjelleberg S | 1997 | Appllied and Environmental Micro- biology1997，63，3： | 1 |
| 8 | Comparisons of diver- sity of bacterial communities associated with three sessile marine eukaryotes显示文摘 | Long'ford SR Tujula NA Crocetti GR Holmes A J Holmstro'' m C Kjelleberg S Steinberg PD & Taylor MW | 2007 | Aquat Microb Ecol2007，48，： | 1 |
| 9 | ComI~osition,uniqueness and variability of the epiphytic bacterial com- munity of the green alga Ulva australis显示文摘 | Burke C Thomas T Lewis M Steinberg P Kjelleberg S | | ISME 201 lb0，5，： | 1 |
| 10 | Marine Pseudoalteromonas species are associated with higher organisms and produce active extracellular compounds 显示文摘 | HOLMSTROM C KJELLEBERG S | 1999 | FEMS Microbiolology Ecology1999，30，4： | 1 |
| 11 | Chemical mediation of bacterial surface colonisation by secondary metaboHtes from the red alga Delisea pulchra显示文摘 | Maximilien R de Nys R Holmstro'm C Gram L Givskov M Crass K Kjelleberg S & Steinberg PD | 1998 | Aquat Microb Ecol1998，15，： | 1 |
| 12 | Chemical cues for surface colonization显示文摘 | STEINBERG P D DE NYS R KJELLEBERG S | 2002 | Journal of Chemical Ecology2002，28，10： | 1 |
| 13 | Devel- opment of novel drugs from marine surface associated microorganisms显示文摘 | PENESYAN A KJELLEBERG S EGAN S | 2010 | Marine Drugs2010，8，3： | 1 |
| 14 | The role of RNA stability during bacterial stress responses and starvation 显示文摘 | Takayama K S A Kjelleberg | 2000 | En- viron Microbiol2000，2，4： | 1 |
| 15 | Surface warfare in the sea显示文摘 | Kjelleberg S Steinberg P | 2001 | Microbiology Today2001，28，： | 1 |
| 16 | Marine Pseudoalteromonas species are associated with higher organisms and produce biological active extracellular agents 显示文摘 | HOLMSTROM C KJELLEBERG S | 1999 | FEMS Microbiol Ecol1999，30，： | 1 |
| 17 | Isolation and characterization of turbot(Scophthalmus maxi- mus)-associated bacteria with inhibitory effects against Vibrio anguillarum显示文摘 | Westerdahl A Olsson J C Kjelleberg S | 1991 | Applied and Environmental Microbiology1991，57，8： | 1 |
| 18 | Competitive interactions in mixed-species biofilms containing the marine bacteri- um Pseudoalteromonas tunicate显示文摘 | Rao D Webb JS Kjelleberg S | 2005 | Applied and Environ- mental Microbiology2005，71，： | 1 |
| 19 | Reinvestigation of the sulfuric acid-catalyzed cyclization of brominated 2-alkyllevulinic acids to 3-alkyl-5-methylene-2 (SH)-furanones显示文摘 | MANNY A J KJELLEBERG S KUMAR N | 1997 | Tetrahedron1997，53，15： | 1 |
| 20 | Correlation between pigmentation and antifouling compounds produced by Pseudoalteromonas tunicata 显示文摘 | Egan S James S Holmstrom C Kjelleberg S | 2002 | Environmental Microbiology2002，4，8： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员