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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Treatability studies of naphthalene in soil,water and air with persulfate activated by iron(Ⅱ)显示文摘Chemical oxidation was applied to an artificially contaminated soil with naphthalene(NAP).Evaluation of NAP distribution and mass reduction in soil,water and air phases was carried out through mass balance.Evaluation of NAP distribution and mass reduction in soil,water and air phases was carried out through mass balance.The importance of the air phase analysis was emphasized by demonstrating how NAP behaves in a sealed system over a 4 hr reaction period.Design of Experiments method was applied to the following variables:sodium persulfate concentration[SP],ferrous sulfate concentration[FeS04],and pH.The system operated with a prefixed solid to liquid ratio of 1:2.The following conditions resulted in optimum NAP removal[SP]=18.37 g/L,[FeSO4]=4.25 g/L and pH=3.00.At the end of the 4 hr reaction,62%of NAP was degraded.In the soil phase,the chemical oxidation reduced the NAP concentration thus achieving levels which comply with Brazilian and USA environmental legislations.Besides the NAP partitioning view,the monitoring of each phase allowed the variabilities assessment over the process,refining the knowledge of mass reduction.Based on NAP distribution in the system,this study demonstrates the importance of evaluating the presence of semi-volatile and volatile organic compounds in the air phase during remediation,so that there is greater control of the system as to the distribution and presence of the contaminant in the environment.The results highlight the importance of treating the contaminant in all its phases at the contaminated site. | Gabriela P.Mendes Vivian M.A.Magalhaes Lélia C·R·Soares Rayanne M.Aranha Claudio A.O.Nascimento Marilda M.G.R.Vianna Osvaldo Chiavone-Filho | 2020 | Journal of Environmental Sciences2020，32，4： | 4 |
| 3 | Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats显示文摘AIM To evaluate the sex-specific effects of a hydroalcoholic extract from Eugenia punicifolia(HEEP) leaves on gastric ulcer healing.METHODS In this rat study involving males, intact(cycling) females, and ovariectomized females, gastric ulcers were induced using acetic acid. A vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Body weight was monitored throughout the treatment period. At the end of treatment, the rats were euthanized and the following in vivo and in vitro investigations were performed: macroscopic examination of the lesion area and organ weights, biochemical analysis, zymography, and evaluation of protein expression levels. Additionally, the concentration-dependent effect of HEEP was evaluated in terms of subacute toxicity and cytotoxicity.RESULTS Compared to the vehicle, HEEP demonstrated a great healing capacity by substantially reducing the ulcerative lesion area in males(52.44%), intact females(85.22%), and ovariectomized females(65.47%), confirming that HEEP accelerates the healing of acetic acidinduced gastric lesions and suggesting that this effect is modulated by female sex hormones. The antiulcer effect of HEEP was mediated by prostaglandin E2 only in male rats. Overall, the beneficial effect of HEEP was the highest in intact females. Notably, HEEP promoted the expression of vascular endothelial growth factor(intact vs ovariectomized females) and decreased the expression of Caspase-8 and Bcl-2(intact female vs male or ovariectomized female). Additionally, HEEP enhanced fibroblast proliferation and migration into a wounded area in vitro, confirming its healing effect. Finally, no sign of subacute toxicity or cytotoxicity of HEEP was observed.CONCLUSION In gastric ulcers, HEEP-induced healing(modulated by female sex hormones; in males, mediated by prostaglandin) involves extracellular matrix remodeling, with gastric mucosa cell proliferation and migration. | Larissa Lucena Périco Vinícius Peixoto Rodrigues Rie Ohara Gabriela Bueno Vania Vasti Alfieri Nunes Raquel Cássia dos Santos Ana Carolina Lima Camargo Luis Antuio Justulin Joior Sérgio Faloni de Andrade Viviane Miranda Bispo Steimbach Luísa Mota da Silva Lúcia Regina Machado da Rocha Wagner Vilegas Catarina dos Santos Clélia Akiko Hiruma-Lima | 2018 | World Journal of Gastroenterology2018，24，38： | 3 |
| 4 | Diabetes mellitus, revascularization and outcomes in elderly patients with myocardial infarction-related cardiogenic shock显示文摘Background The prognostic role of diabetes mellitus(DM)in elderly patients with myocardial infarction-related cardiogenic shock(MI-CS)remains controversial.Little information exists about the impact of intensive cardiac care unit(ICCU)and revascularization on outcomes of elderly patients with MI-CS.We aimed to assess the prognostic impact of DM according to age in patients with MI-CS,and to analyze the impact ICCU management and revascularization on in-hospital mortality in MI-CS patients at older ages.Methods Discharge episodes with diagnosis of CS associated with MI were selected from the Spanish National Health System’s Basic Data Set.Centers were classified according to their availability of ICCU.Main outcome measured was in-hospital mortality.Results A total of 23,590 episodes of MI-CS were identified,of whom 12,447(52.8%)were in patients aged≥75 years.The impact of DM on in-hospital mortality was different among age subgroups.While in younger patients,DM was associated to a higher mortality risk(0.52 vs.0.47,OR=1.12,95%CI:1.06–1.18,χ^2<0.001),this association became non-significant in older patients(0.76 vs.0.81,χ^2=0.09).Adjusted mortality rate of MI-CS aged≥75 years was lower in patients admitted to hospitals with ICCU(adjusted mortality rate:74.2%vs.77.7%,P<0.001)and in patients undergoing revascularization(74.9%vs.77.3%,P<0.001).Conclusions Prognostic impact of DM in patients with MI-CS was different according to age,with a significantly lower impact at older ages.The availability of ICCU and revascularization were associated with better outcomes in these complex patients. | Miquel Gual Albert Ariza-Solé María García Márquez Cristina Fernández JoséL Bernal Francesc Formiga María-Isabel Barrionuevo JoséC Sánchez-Salado Victòria Lorente Júlia Pascual Isaac Llaó Oriol Alegre Angel Cequier Javier Elola | 2020 | Journal of Geriatric Cardiology2020，17，10： | 2 |
| 5 | 显示文摘 | Pang R Lia C Y Shi L L | 2009 | Journal of Physics and Chemistry of Solids2009，70，： | 1 |
| 6 | Experimental study on thermal performance of a solar chimney combined with PCM 显示文摘 | Li Y C Lia S L | 2014 | Applied Energy2014，114，： | 1 |
| 7 | Differential gene expres- sion signature between primary and metastatic head and neck squamous cell carcinoma 显示文摘 | Liu C J Lia T Y Kuo L T | 2008 | J Pathol2008，214，4： | 1 |
| 8 | A new method for deposition of cubic Ta diffusion barrier for Cu metallization显示文摘 | Yuan Z L Zhanga D H Lia C Y | 2003 | Thin Solid Films2003，434，： | 1 |
| 9 | Treatment of oily wastewater by organic-inorganic composite tubular ultrafihration(UF) membranes 显示文摘 | Lia Y S Yana L Xiang C B | 2012 | Desalination2012，196，： | 1 |
| 10 | Anti-diabetic effect of American ginseng may not be linked to antioxidant activity:Comparison between American ginseng and Scutellaria ba icalensis using an ob/ob mice model显示文摘 | Xie J T Wang C Z Lia X L | | 0，，05： | 1 |
| 11 | Carbun isotope signatures of fatty acids in Geobacter metallireducens and Shewanella algae 显示文摘 | Chuanlun L Zhang Lia Yiliang Qi Ye Jon Fong Aaron D Peacock Elizabeth Blunt Fang Jiasong Derek R Eovley David C White | 2003 | Chemical Geology2003，195，： | 1 |
| 12 | The protection coatings of NdFeB magnets by AI and A1 (Fe) 显示文摘 | Qin C D LiA S K NgD H L | 1996 | Journal of Applied Physics1996，79，8： | 1 |
| 13 | Calpain-3 gene expression is decreased during experimental cancer cachexia显示文摘 | S??lvia Busquets Cèlia Garc??a-Mart??nez Belén Alvarez Neus Carbó Francisco J López-Soriano Josep M Argilés | 2000 | BBA - General Subjects2000，，1： | 1 |
| 14 | Metabolic profiles of sunflower genotypes with contrasting response to Sclerotinia sclerotiorum infection显示文摘 | Peluffo L Lia V Troglia C Maringolo C Norma P Escande A Esteban Hopp H Lytovchenko A Fernie A R Heinz R Carrari F | 2010 | Phytochemistry2010，71，1： | 1 |
| 15 | Enabling information sharing between E-commerce systems for construction material procurement显示文摘 | Stephen C W Kong Heng Lia Tim P L Hunga | 2004 | Automation in Construction2004，，13： | 1 |
| 16 | Bone marrow mononuclear cell therapy for patients with cirrhosis: a Phase 1 study显示文摘 | Bianca G.Couto Regina C. dos SantosGoldenberg Léa M. B.da Fonseca JamesThomas BiancaGutfilen Célia M. C.Resende FelicianoAzevedo Daniel R.Mercante André L. MoreiraTorres Henrique S. M.Coelho AngeloMaiolino Alessandra L. dos AnjosAlves Juliana V.Dias Maria | 2011 | Liver International2011，，3： | 1 |
| 17 | Study of flowerlike Ce02 microspheres used as catalyst supports for CO oxidation reaction 显示文摘 | Sun C W Lia H Chen L Q | 2007 | J Phy Chem Solid2007，68，： | 1 |
| 18 | Over expression of E2F1 inhibitsprogression of gastric cancer in vitro 显示文摘 | Xie Y Wang C Lia L | 2009 | Cell BiologyInternational2009，33，6： | 1 |
| 19 | An augmented approach for Stokes equations with a discontinuous viscosity and singular forces 显示文摘 | Lia Z L Kazufumi I Lai M C | 2007 | Computers and Fluids2007，36，3： | 1 |
| 20 | Nitrogen-containing compounds from Salvia miltiorrhiza显示文摘 | Don M J Shen C C Lia T L | 2005 | J Nat Prod2005，68，7： | 1 |
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