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| 1 | Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems显示文摘The CRISPR/Cas system has been extensively applied to make precise genetic modifications in various organisms. Despite its importance and widespread use, large-scale mutation screening remains time-consuming, labour-intensive and costly. Here, we developed Hi-TOM(available at http://gffzz103f3ce5d9b14419h56q60vc6556n6x60.ffgz.tsg.suse.edu.cn/hi-tom/), an online tool to track the mutations with precise percentage for multiple samples and multiple target sites. We also described a corresponding next-generation sequencing(NGS) library construction strategy by fixing the bridge sequences and barcoding primers. Analysis of the samples from rice, hexaploid wheat and human cells reveals that the Hi-TOM tool has high reliability and sensitivity in tracking various mutations, especially complex chimeric mutations frequently induced by genome editing. Hi-TOM does not require special design of barcode primers,cumbersome parameter configuration or additional data analysis. Thus, the streamlined NGS library construction and comprehensive result output make Hi-TOM particularly suitable for high-throughput identification of all types of mutations induced by CRISPR/Cas systems. | Qing Liu Chun Wang Xiaozhen Jiao Huawei Zhang Lili Song Yanxin Li Caixia Gao Kejian Wang | 2019 | Science China(Life Sciences)2019，62，1： | 37 |
| 2 | TRAIL receptor mediates inflammatory cytokine release in an NF-κB-dependent manner显示文摘在现在的文章,我们报导 DR4 或 DR5 overexpression 戏剧性地在 293T, MDA-MB-231 和 HCT-116 房间以一种 NF-魏 B 依赖者方式激活煽动性的 cytokines IL-8, TNF- 伪, CCL20, MIP-2 和 MIP-1 尾的版本。我们显示出调停受体的信号是的那死亡细胞外域无关,而 DR4 细胞内部的领域的 overexpression 的效果是少得多有势力。表明串联的 TRADD-TRAF2-NIK-IKK 伪 / 尾,在导致 TNF 的 NF-魏 B 激活起一个必要作用,被发现涉及肿瘤坏死因素相关导致 apoptosis ligand (小道) 调停受体的信号 transduction。表明小径的 FADD-caspase,被报导了主要与 apoptosis 有关,作为与表明串联的 TRADD-TRAF2-NIK-IKK 伪 / 尾为 DR4 或 DR5 调停 overexpression 的 NF-魏 B 激活和 cytokine 分泌物和串音是必要的被识别。而且, DR5 论战的抗体(AD5-10 ) 触发了煽动性的 cytokine 版本。这些数据和以前的报告,提供小道和小道受体在发炎起一个重要作用的充分证据。 | Wanhu Tang Weimin Wang Yaxi Zhang Shilian Liu Yanxin Liu Dexian Zheng | 2009 | Cell Research2009，19，6： | 14 |
| 3 | Clean Energy Consumption of Power Systems Towards Smart Agriculture: Roadmap, Bottlenecks and Technologies显示文摘Over the past decades,both agriculture and power systems have faced serious problems,such as the power supply shortage in agriculture,and difficulties of clean energy consump-tion in the power system.To address and overcome these issues,this paper proposes an idea to combine smart agriculture and clean energy consumption,use surplus clean energy to supply agriculture production,and utilize smart agriculture to support power system with clean energy penetration.A comprehensive review has been conducted to first depict the roadmap of coupling a agriculture-clean energy system,analyze their feasibilities and advantages.The recent technologies and bottlenecks are summa-rized and evaluated for the development of a combined system consisting of smart agriculture production and clean energy consumption.Several case studies are introduced to explore the mutual benefits of agriculture-clean energy systems in both the energy and food industries. | Junyong Liu Yanxin Chai Yue Xiang Xin Zhang Si Gou Youbo Liu | 2018 | CSEE Journal of Power and Energy Systems2018，4，3： | 9 |
| 4 | Near-infrared reflectance spectroscopy reveals wide variation in major components of sesame seeds from Africa and Asia显示文摘Sesame is an important oilseed crop in Africa and Asia, owing to its high nutritional quality seed and market value. Variation in sesame seed components including oil, oleic acid,linoleic acid, and protein was investigated by near-infrared reflectance spectrometry in 139 samples collected from different countries. Oil and protein contents were between 40.8%and 60.3%(mean 53.0%) and 15.5% to 25.5%(mean 20.4%), respectively. Linoleic acid,ranging from 31.8% to 52.4%(mean 46%) was more abundant than oleic acid(31.8%–50.6%,mean 38.1%). Light-seeded samples displayed higher nutritional quality, as they were richer in oil, protein, and linoleic acid than dark seeds. Samples from Africa had higher oil and linoleic acid contents, while Asian samples had higher oleic content. The analysis revealed West African sesame cultivars containing especially high levels of seed components, which may command high market values. Two clusters of sesame samples grouped by seed composition were obtained, including one cluster with high oil and oleic acid content and the other with high protein and linoleic acid content. This study revealed that sesame samples from Africa and Asia harbor high variation for major seed components and also provided background information for breeding high-nutrition varieties according to the demands of sesame seed markets. | Komivi Dossa Xin Wei Marème Niang Pan Liu Yanxin Zhang Linhai Wang Boshou Liao Ndiaga Cissé Xiurong Zhang Diaga Diouf | 2018 | The Crop Journal2018，6，2： | 7 |
| 5 | On the modification of hypereutectic Al-Si alloys using rare earth Er显示文摘Hypereutectic Al-17.5Si (wt pct) and Al-25Si (wt pct) alloys with various content of rare earth Er were prepared by conventional casting technique. The effect of Er on the microstructure and properties of the hypereutectic Al-Si alloys was investigated using optical microscopy, scanning electron microscopy as well as friction and wear tests. It was found that optimal amount of Er exists in modifying the hypereutectic Al-Si alloys. An appropriate addition of Er improved the anti-wear properties, and reduced the friction coefficient of the hypereutectic Al-Si alloys studied. The primary silicon crystals in the hypereutectic Al-Si alloys were refined with the suitable addition of Er element. The modification mechanism was also discussed in this paper. | Pengfei XING Bo GAO Yanxin ZHUANG Kaihua LIU Ganfeng TU | 2010 | Acta Metallurgica Sinica(English Letters)2010，23，5： | 7 |
| 6 | Classification and Facies Sequence Model of Subaqueous Debris Flows显示文摘Objective Debris flows are cohesive sediment gravity flows which occur in both subaerial and subaqueous settings.Compared to subaerial debris flows which have been well studied as a geological hazard,subaqueous debris flows showing complicated sediment composition and sedimentary processes were poorly understood.The main objective | XIAN Benzhong LIU Jianping DONG Yanlei LU Zhiyong HE Yanxin WANG Junhui | 2017 | Acta Geologica Sinica(English Edition)2017，91，2： | 6 |
| 7 | Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast显示文摘Tumor necrosis factor-related apop-tosis-inducing ligand (TRAIL) induces selectively apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. A chloroplast expres-sion vector, p64TRAIL, containing the cDNA coding for the soluble TRAIL (sTRAIL), was constructed with clpP-trnL-petB-chlL-rpl23-rpl2 as Chlamydomonas reinhardtii plastid homologous recombinant frag-ments and spectinomycin-resistant aadA gene as a select marker. The plasmid p64TRAIL was trans-ferred into the chloroplast genome of C. reinhardtii by the biolistic method. Three independently trans-formed lines were obtained by 100 mg/L spectino-mycin selection. PCR amplification, Southern blot analysis of the sTRAIL coding region DNA and culti-vation cells in the dark all showed that the exogenous DNA had been integrated into chloroplast genome of C. reinhardtii. Western blot analysis showed that human soluble TRAIL was expressed in C. reinhardtii chloroplast. The densitometric analysis of Western blot indicated that the expressed human sTRAIL protein in the chloroplasts of C. reinhardtii accounted for about 0.43%―0.67% of the total soluble proteins. These experimental results demonstrated the possi-bility of using transgenic chloroplasts of green alga as bioreactors for production of biopharmaceuticals. | YANG Zongqi LI yinü CHEN Feng LI Dong ZHANG Zhifang LIU Yanxin ZHENG Dexian WANG Yong SHEN Guifang | 2006 | Chinese Science Bulletin2006，51，14： | 4 |
| 8 | Current Understanding on the Immunological Functions of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand显示文摘Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. The killing specificity of TRAIL has brought great interests to develop a novel apoptosis-based anti-tumor agent for clinical application. TRAIL is expressed in many normal tissues and cells, such as liver, brain, kidney, heart, colon, lung, and testis. However, immunological and physiological functions of TRAIL in vivo have not been understood well. In the present paper we summarized the progress in the research on immunological functions of TRAIL. | Wei Wei Yanxin Liu Dexian Zheng | 2005 | Cellular & Molecular Immunology2005，2，4： | 4 |
| 9 | Weaving 3D highly conductive hierarchically interconnected nanoporous web by threading MOF crystals onto multi walled carbon nanotubes for high performance Li-Se battery显示文摘Lithium-selenium(Li-Se)battery has attracted growing attention.Nevertheless,its practical application is still impeded by the shuttle effect of the formed polyselenides.Herein,we report in-situ hydrothermal weaving the three-dimensional(3 D)highly conductive hierarchically interconnected nanoporous web by threading microporous metal organic framework MIL-68(Al)crystals onto multi-walled carbon nanotubes(MWCNTs).Such 3 D hierarchically nanoporous web(3 D MIL-68(Al)@MWCNTs web)with a very high surface area,a large amount of micropores,electrical conductivity and elasticity strongly traps the soluble polyselenides during the electrochemical reaction and significantly facilitates lithium ion diffusion and electron transportation.Molecular dynamic calculation confirmed the strong affinity of MIL-68(Al)for the adsorption of polyselenides,quite suitable for Li-Se battery.Their hexahedral channels(1.56 nm)are more efficient for the confinement of polyselenides and for the diffusion of electrolytes compared to their smaller triangular channels(0.63 nm).All these excellent characteristics of 3 D MIL-68(Al)@MWCNTs web with suitable confinement of a large amount of selenium and the conductive linkage between MIL-68(Al)host by MWCNTs result in a high capacity of 453 m Ah/g at 0.2 C with 99.5%coulombic efficiency after 200 cycles with significantly improved cycle stability and rate performance.The 3 D MIL-68(Al)@MWCNTs web presents a good performance in Li-Se battery in term of the specific capacity and cycling stability and also in terms of rate performance compared with all the metal-organic framework(MOF)based or MOF derived porous carbons used in Li-Se battery. | Chao Li Yingying Wang Hongyan Li Jing Liu Jianping Song Luca Fusaro Zhi-Yi Hu Yanxin Chen Yu Li Bao-Lian Su | 2021 | Journal of Energy Chemistry2021，30，8： | 4 |
| 10 | Predicting geogenic groundwater fluoride contamination throughout China显示文摘Endemic fluorosis exists in almost all provinces of China.The long-term ingestion of groundwater containing high concentrations of fluoride is one of the main causes of fluoro-sis.We used artificial neural network to model the relationship between groundwater fluo-ride concentrations from throughout China and environmental variables such as climatic,geological.and soil parameters as proxy predictors.The results show that the accuracy and area under the receiver operating characteristic curve of the model in the test dataset are 80.5%and 0.86%,respectively,and climatic variables are the most effective predictors.Based on the artificial neural network model,a nationwide prediction risk map of fluoride concen-trations exceeding 1.5 mg/L with a 0.5×0.5 arc minutes resolution was generated.The high risk areas are mainly located in western provinces of Xinjiang,Tibet,Qinghai,and Sichuan,and the northern provinces of Inner Mongolia,Hebei and Shandong.The total number of people estimated to be potentially at risk of fluorosis due to the use of untreated high flu-oride groundwater as drinking water is about 89 million,or 6%of the population.The high fluoride groundwater risk map helps the authorities to prioritize areas requiring mitigation measures and thus facilitates the implementation of water improvement and defluorida-tion projects. | Hailong Cao Xianjun Xie Yanxin Wang Hongxing Liu | 2022 | Journal of Environmental Sciences2022，34，5： | 3 |
| 11 | Synthesis and biological evaluation of new 13-n-nonylprotoberberine derivatives as antitubercular agents显示文摘Tuberculosis is a serious threat to public health throughout the world.A series of new l3-n-nonylprotoberberine derivatives was designed,synthesized and evaluated for anti-mycobacterial activity against Mycobacterium tuberculosis strain H_(37)Rv.Several compounds(2,11a-c,11g,13d,15c)exhibited excellent anti-tubercular activity with an MIC below 1.0μg/mL.Notably,compound 13d showed potential antibacterial effect against both drug-susceptible and multidrug-resistant(MDR)M.tuberculosis with MIC ranges of 0.0625-1.0μg/mL.These results suggest a mode of action different from that of the current anti-mycobacterial drugs rifampicin and isoniazid.Hence,compound 13d is an attractive lead compound for the development of new antitubercular agents. | Yinghong Li Yanxin Liu Yanxiang Wang Sheng Tang Danqing Song | 2013 | Acta Pharmaceutica Sinica B2013，3，1： | 3 |
| 12 | Status Quo and Development Prospects of Vertical Greening in Urban Districts of Chongqing City显示文摘On the basis of investigating vertical greening in urban districts of Chongqing City, major problems were analyzed, dominant greening patterns were summarized, and major plant species suitable for the vertical greening of urban districts in Chongqing City were given. On this basis, key technologies and development prospects of the vertical greening were explored. | MA Lihui XIE Yingzan FANG Wen HUANG Shiyou LIU Yanxin | 2013 | Journal of Landscape Research2013，5，5： | 2 |
| 13 | The protective role of hydrogen-rich saline in experimental liver injury in mice显示文摘 | HanYong Sun Lei Chen WeiPing Zhou Liang Hu Liang Li QianQian Tu YanXin Chang Qu Liu XueJun Sun MengChao Wu HongYang Wang | 2010 | Journal of Hepatology2010，，3： | 2 |
| 14 | A Novel Anti-human DR5 Monoclonal Antibody with Tumoricidal Activity Induces Caspase-dependent and Caspase-independent Cell Death显示文摘 | Yabin Guo Caifeng Chen Yong Zheng Jinchun Zhang Xiaohui Tao Shilian Liu Dexian Zheng Yanxin Liu | 2006 | 中国生物学文摘2006，20，1： | 2 |
| 15 | Retrieving Drill Bit Seismic Signals Using Surface Seismometers显示文摘Seismic while drilling(SWD) is an emerging borehole seismic imaging technique that uses the downhole drill-bit vibrations as seismic source.Without interrupting drilling,SWD technique can make near-real-time images of the rock formations ahead of the bit and optimize drilling operation,with reduction of costs and the risk of drilling.However,the signal to noise ratio(SNR) of surface SWD-data is severely low for the surface acquisition of SWD data.Here,we propose a new method to retrieve the drill-bit signal from the surface data recorded by an array of broadband seismometers.Taking advantages of wavefield analysis,different types of noises are identified and removed from the surface SWD-data,resulting in the significant improvement of SNR.We also optimally synthesize seismic response of the bit source,using a statistical cross-coherence analysis to further improve the SNR and retrieve both the drill-bit direct arrivals and reflections which are then used to establish a reverse vertical seismic profile(RVSP) data set for the continuous drilling depth.The subsurface images derived from these data compare well with the corresponding images of the three-dimension surface seismic survey cross the well. | Linfei Wang Huaishan Liu Siyou Tong Yanxin Yin Lei Xing Zhihui Zou Xiugang Xu | 2015 | Journal of Earth Science2015，26，4： | 2 |
| 16 | Description of rotational properties of the superheavy nucleus ^(256,258,260)Rf by projected shell model显示文摘Band structure of the Z=104 nucleus256Rf has been a recent research focus in nuclear physics.We performed projected shell model calculations for this and some other neighboring Rf isotopes.Specifically,we studied rotational properties of these nuclei and showed that variations in dynamic moment of inertia were sensitive to single-particle structures,which may be useful information for locating the anticipated island of stability.Electromagnetic transition properties were also calculated,and measurable quantities were predicted for further experimental test. | Zhiyi Zhou Yanxin Liu Yingchun Yang Fangqi Chen Yang Sun | 2014 | Chinese Science Bulletin2014，59，29： | 2 |
| 17 | Shallow Water Body Data Processing Based on the Seismic Oceanography显示文摘Physical properties of sea water,such as salinity,temperature,density and acoustic velocity,could be demarcated through degradation of energy caused by water absorption,attenuation and other factors.To overcome the challenging difficulties in the quick monitoring of these physical properties,we have explored the high resolution marine seismic survey to instantly characterize them.Based on the unique wavefield propagating in the sea water,we have developed a new approach to suppress the noise caused by the shallow sea water disturbance and obtain useful information for estimating the sea water structure.This approach improves seismic data with high signal-to-noise ratio and resolution.The seismic reflection imaging can map the sea water structure acoustically.Combined with the knowledge of local water body structure profile over years,the instant model for predicting the sea water properties could be built using the seismic data acquired from the specially designed high precision marine seismic acquisition.This model can also be updated with instant observation and the complete data processing system.The present study has the potential value to many applications,such as 3D sea water monitoring,engineering evaluation,geological disaster assessment and environmental assessment. | LIU Huaishan HU Yi YIN Yanxin WANG Linfei TONG Siyou MA Hai | 2013 | Journal of Ocean University of China2013，12，3： | 2 |
| 18 | Mesencephalic astrocyte-derived neurotrophic factor reduces cell apoptosis via upregulating HSP70 in SHSY-5Y cells显示文摘Background:Mesencephalic astrocyte-derived neurotrophic factor(MANF)is a new candidate growth factor for dopaminergic neurons against endoplasmic reticulum stress(ER stress).HSP70 family,a chaperon like heat shock protein family,was proved to be involved in the MANF induced survival pathway in 6-OHDA treated SHSY-5Y cells.However,the ER stress relative transcriptome,in MANF signaling cascades is still investigated.The involvement of HSP70,a 70kd member of HSP70 family,need further to be verified.Methods:The cell apoptosis was assayed by MTT,TUNEL staining and western blot of cleaved Caspase-3.The differentially expressed genes in SHSY-5Y cells under different conditions(control,6-OHDA,6-OHDA+MANF)were investigated by RNA-seq.Expression of HSP70 was further confirmed by real-time PCR.RNAi knockdown for HSP70 was performed to investigate the role of HSP70 in the MANF signaling pathway.Results:MANF inhibits 6-OHDA-induced apoptosis in SHSY-5Y cells.Six ER stress relative genes(HSP70,GRP78,xbp-1,ATF-4,ATF-6,MAPK)were found enriched in 6-OHDA+MANF treatment group.HSP70 was the most significantly up-regulated gene under 6-OHDA+MANF treatment in SHSY-5Y cells.RNAi knockdown for HSP70 inhibits the protective effects of MANF against 6-OHDA toxicity in SHSY-5Y cells.Conclusion:MANF exerts a protective role against 6-OHDA induced apoptosis in SHSY-5Y cells via up-regulating some ER stress genes,including HSP70 family members.The HSP70 expression level plays a key role in MANFmediated survival pathway. | Hui Sun Ming Jiang Xing Fu Qiong Cai Jingxing Zhang Yanxin Yin Jia Guo Lihua Yu Yun Jiang Yigang Liu Liang Feng Zhiyu Nie Jianmin Fang Lingjing Jin | 2017 | Translational Neurodegeneration2017，6，1： | 2 |
| 19 | I-Neat:An Intelligent Framework for Adaptive Virtual Machine Consolidation显示文摘With the increasing use of cloud computing,high energy consumption has become one of the major challenges in cloud data centers.Virtual Machine(VM)consolidation has been proven to be an efficient way to optimize energy consumption in data centers,and many research works have proposed to optimize VM consolidation.However,the performance of different algorithms is related with the characteristics of the workload and system status;some algorithms are suitable for Central Processing Unit(CPU)-intensive workload and some for web application workload.Therefore,an adaptive VM consolidation framework is necessary to fully explore the potential of these algorithms.Neat is an open-source dynamic VM consolidation framework,which is well integrated into OpenStack.However,it cannot conduct dynamic algorithm scheduling,and VM consolidation algorithms in Neat are few and basic,which results in low performance for energy saving and Service-Level Agreement(SLA)avoidance.In this paper,an Intelligent Neat framework(I-Neat)is proposed,which adds an intelligent scheduler using reinforcement learning and a framework manager to improve the usability of the system.The scheduler can select appropriate algorithms for the local manager from an algorithm library with many load detection algorithms.The algorithm library is designed based on a template,and in addition to the algorithms of Neat,I-Neat adds six new algorithms to the algorithm library.Furthermore,the framework manager helps users add self-defined algorithms to I-Neat without modifying the source code.Our experimental results indicate that the intelligent scheduler and these novel algorithms can effectively reduce energy consumption with SLA assurance. | Yanxin Liu Yao Zhao Jian Dong Lianpeng Li Chunpei Wang Decheng Zuo | 2022 | Tsinghua Science and Technology2022，27，1： | 2 |
| 20 | Heat transfer enhancement of micro oscillating heat pipes with self-rewetting fluid显示文摘 | Hu Yanxin Liu Tengqing Wang Shuangfeng | 2014 | International Journal of Heat and Mass Transfer2014，70，： | 1 |
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