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| 1 | Role of LPS/CD14/TLR4-mediated inflammation in necrotizing enterocolitis:Pathogenesis and therapeutic implications显示文摘AIM:To establish the roles of lipopolysaccharide (LPS)/CD14/toll-like receptor 4 (TLR4)-mediated inflammation in a rat model of human necrotizing enterocolitis (NEC).METHODS: Six pairs of intestinal samples from human NEC were collected before and after recovery for histological and molecular analysis of inflammatory cytokines and signaling components. In the rat NEC model, we isolated 10-cm jejunum segments and divided them into six groups (n=6) for sham operation, treatment with LPS, bowel distension, combined bowel distension and LPS stimulation, and two therapeutic groups. The potential eff icacy of a recombinant CD18 peptide and a monoclonal CD14 antibody was evaluated in the latter two groups. The serum and tissue levels of several inflammatory mediators were quantified by real-time polymerase chain reaction, ELISA and immunoblotting.RESULTS: Human acute phase NEC tissues displayed significant increases (P<0.05) in levels of TLR4, CD14, myeloid differentiation protein (MD)-2, tumor necrosis factor (TNF)-α and nuclear factor-κB when compared to those after recovery. The histological and inflammatory picture of human NEC was reproduced in rats that were treated with combined bowel distension and LPS, but not in the sham-operated and other control rats. Serum levels of interleukin-6 and TNF-α were also elevated. The NEC pathology was attenuated by treating the NEC rats with a monoclonal CD14 antibody or an LPS-neutralizing peptide.CONCLUSION:LPS and distension are required to produce the histological and inflammatory features of NEC. A potential treatment option is blocking LPS activation and leukocyte infi ltration. | Kwong L Chan Kwong F Wong John M Luk | 2009 | World Journal of Gastroenterology2009，15，38： | 17 |
| 2 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 3 | Effect of aerobic and anaerobic exercises on glycemic control in type 1 diabetic youths显示文摘AIM:To evaluate the long-term effect of aerobic and/or anaerobic exercise on glycemic control in youths with type 1 diabetes.METHODS:Literature review was performed in spring and summer 2014 using Pub Med/MEDLINE,Google Scholar,Scopus,and Science Direct with the following terms:aerobic,anaerobic,high-intensity,resistance,exercise/training,combined with glycemic/metabolic control,glycated haemoglobin A1c(Hb A1c) and type 1diabetes.Only peer-reviewed articles in English were included published in the last 15 years.It was selected from 1999 to 2014.Glycemic control was measured with Hb A1 c.Studies with an intervention lasting at least 12 wk were included if the Hb A1 c was measured before and after the intervention.RESULTS:A total of nine articles were found,and they were published between the years of 2002-2011.The sample size was 401 diabetic youths(166 males and 235 females) with an age range of 10-19 years except one study,in which the age range was 13-30 years.Study participants were from Australia,Tunisia,Lithuania,Taiwan,Turkey,Brazilia,Belgium,Egypt and France.Four studies were aerobic-based,four were combined aerobic and anaerobic programs,and one compared aerobic exercise to anaerobic one.Available studies had insufficient evidence that any type of exercise or combined training would clearly improve the glycemic control in type 1 diabetic youth.Only three(two aerobic-based and one combined) studies could provide a significant positive change in glycemic control.CONCLUSION:The regular physical exercise has several other valuable physiological and health benefits that justify the inclusion of exercise in pediatric diabetes treatment and care. | Andrea Lukács László Barkai | 2015 | World Journal of Diabetes2015，6，3： | 5 |
| 4 | Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa显示文摘Background: Lycopene(LYC) is a natural carotenoid with powerful reactive oxygen species(ROS) scavenging activities. The aim of this study was to investigate if lycopene has the ability to reverse ROS-mediated alterations to the motility, viability and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate(Fe AA). Spermatozoa were washed out of fresh bovine semen, suspended in 2.9 % sodium citrate and subjected to LYC treatment(0.25, 0.5, 1 or 2 mmol/L) in the presence or absence of Fe AA(150 μmol/L Fe SO_4 and 750 μmol/L ascorbic acid) during a 6 h in vitro culture. Spermatozoa motion characteristics were assessed using the Sperm Vision?computer-aided sperm analysis(CASA) system. Cell viability was examined with the metabolic activity(MTT) assay,ROS generation was quantified via luminometry and the nitroblue-tetrazolium(NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the in vitro culture to investigate the intracellular activity of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx) as well as the concentrations of glutathione(GSH) and malondialdehyde(MDA).Results: FeA A treatment led to a reduced spermatozoa motility(P < 0.001), viability(P < 0.001) and a decline of the antioxidant capacity of spermatozoa(P < 0.001) but increased the ROS generation(P < 0.001), superoxide production(P < 0.001) and lipid peroxidation(P < 0.001). LYC administration resulted in a preservation of the spermatozoa motion parameters(P < 0.001), mitochondrial activity(P < 0.001) and antioxidant characteristics(P < 0.001 with respect to SOD;P < 0.01 in relation to CAT; P < 0.05 as for GPx and GSH) with a concentration range of 1 and 2 mmol/L LYC revealed to be the most effective.Conclusions: Our results suggest that LYC exhibits significant ROS-scavenging and antioxidant properties which may prevent spermatozoa alterations caused by oxidative stress, and preserve the functionality of male reproductive cells. | Eva Tvrdá Anton Kovácik Eva Tusimová Dusan Paál Alica Mackovich Jakhongir Alimov Norbert Lukác | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 3 |
| 5 | Renal artery aneurysm at the anastomosis after kidney transplantation显示文摘 | Asztalos L Olvasztó S Fedor R Szabó L Balázs G Lukács G | 2006 | Transplant Proc2006，38，： | 1 |
| 6 | Effect of grain size on the high cycle fatigue behavior of polycrystalline copper显示文摘 | Lukás P Kunz L | 1987 | Materials Science and Engineering1987，85，： | 1 |
| 7 | Hong Kong and China:The Cash Mentality Revisited显示文摘 | CHIU R K LUK V W TANG T L | 2001 | Compensation and Benefits Review2001，33，3： | 1 |
| 8 | A Wideband dual-polarized 1- probe stacked patch antenna array显示文摘 | LAU K L LUK K M | 2007 | IEEE Antennas and Wireless Propagation Letters2007，6，99： | 1 |
| 9 | Using liquid crystals to amplify protein-receptor interactions:Design of surfaces with nanometer-scale topography that present histidine-tagged protein receptors显示文摘 | Luk Y Y Tingey M L Hall D J | 2003 | Langmuir2003，19，5： | 1 |
| 10 | Endothelium-derived hyperpolarizing factor in vascular physiology and cardiovascular disease显示文摘 | Luks L Agewall S Kublickiene K | 2009 | Atheroscle- rosis2009，202，2： | 1 |
| 11 | Estrogen receptor alpha CA dinucleotide repeat polymorphism is associated with rate of bone loss in perimenopausal women and bone mineral density and risk of osteoporotic fractures in postm- enopausal women 显示文摘 | LAI B M CHEUN C L LUK K D | 2008 | Osteoporos Int2008，19，4： | 1 |
| 12 | Effect of light energy on peroxide tooth bleaching显示文摘 | Luk K Tam L Hubert M | 2004 | J Am Dent Assoc2004，135，2： | 1 |
| 13 | Orientational behavior of thermotropic liquid crystals on surfaces presenting electrostatically bound vesicu-stomatitis virus显示文摘 | Espinoza L A T Schumann R K Luk Y Y | 2004 | Langmuir2004，20，6： | 1 |
| 14 | Construction of tunable radial basis function networks using orthogonal forward selection 显示文摘 | Chen S Hong X Luk B L | 2009 | IEEE Transactions on Systems Man and Cybernetics Part B-Cybernetics2009，39，2： | 1 |
| 15 | Adaptive simulated annealing for optimization in signal processing applications显示文摘 | Chen S Luk B L | 1999 | Signal Processing1999，79，1： | 1 |
| 16 | Effect of light energy on peroxide tooth bleaching显示文摘 | TAM L HUBERT M | 2004 | J Am Dent Assoc2004，135，2： | 1 |
| 17 | Effect of segmental artery ligation on the blood supply of the thoracic spinal cord during anterior spinal surgery:a quantitative histomorphological fresh cadaver study显示文摘 | Yuan L Ni G X Luk K K | 2005 | Spine2005，30，5： | 1 |
| 18 | Addition of ex- ogenous α-synuclein preformed fibrils to primary neuronal cultures to seed recruitment of endogenous α-synuclein to Lewy body and Lewy neurite-like aggregates 显示文摘 | Volpicelli-Daley L A Luk K C Lee V M | 2014 | Nat Pro- toc2014，9，9： | 1 |
| 19 | A stronger bicortical sacral pedicle screw fixation through the S1 endplate: an In vitro cyclic loading and pull-out force evaluation显示文摘 | Luk KD Chen L Lu WW | 2005 | Spine2005，30，5： | 1 |
| 20 | A novel wide-band circularly polarized patch antenna based on L-probe and aperture-coupling techniques显示文摘 | K L Lau K M Luk | 2005 | IEEE Transactions on Antennas and Propagation2005，53，1： | 1 |
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