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| 1 | DGGE法与常规培养法对稻田蓝细菌多态性分析结果比较显示文摘研究运用蓝细菌和硅藻16SrDNA特异引物,将晚季水稻生长后期稻田土壤中提取的总DNA进行PCR扩增后,以DGGE技术对PCR产物进行分析结果表明,14条DGGE带经克隆测序,经NCBI基因库比对得晚季水稻生长后期存在10种蓝细菌,包括4种Leptolyngbya、1种Chamaesiphon、1种Nostoc、1种Oscillatoria、2种Syne-chococcus和1种Chroococcidiopsis。同层不同位置土壤中蓝细菌种群亦有所不同,但每个取样点都有一些特有的蓝细菌种类。用常规方法对同一稻田土壤样品进行分离培养,根据蓝细菌鉴定图谱观察到类似Lyngbya、Oscillatori-a、Chroococcidiopsis及Nostoc的蓝细菌,但显微镜下无法准确分类。比较结果表明采用DGGE法比常规培养法能更准确进行蓝细菌多态性鉴定。 | 宋铁英 林智敏 郑伟文 LOTTA Martensson ULLA Rusmussen | 2005 | 中国生态农业学报2005,13,3: | 4 |
| 2 | Ca^(2+) involvement in activation of extracellular-signal- regulated-kinase 1/2 and m-calpain after axotomy of the sciatic nerve显示文摘Detailed mechanisms behind regeneration after nerve injury, in particular signal transduction and the fate of Schwann cells(SCs), are poorly understood. Here, we investigated axotomy-induced activation of extracellular-signal-regulated kinase-1/2(ERK1/2; important for proliferation) and m-calpain in vitro, and the relation to Ca2+ deletion and Schwann cell proliferation and death after rat sciatic nerve axotomy. Nerve segments were cultured for up to 72 hours with and without ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid(EGTA). In some experiments, 5-bromo-2′-deoxyuridine(Brd U) was added during the last 24 hours to detect proliferating cells and propidium iodide(PI) was added at the last hour to detect dead and/or dying cells. Immunohistochemistry of sections of the cultured nerve segments was performed to label m-calpain and the phosphorylated and activated form of ERK1/2. The experiments revealed that immunoreactivity for p-ERK1/2 increased with time in organotypically cultured SCs. p-ERK1/2 and m-calpain were also observed in axons. A significant increase in the number of dead or dying SCs was observed in nerve segments cultured for 24 hours. When deprived of Ca2+, activation of axonal m-calpain was reduced, whereas p-ERK1/2 was increased in SCs. Ca2+ deprivation also significantly reduced the number of proliferating SCs, and instead increased the number of dead or dying SCs. Ca2+ seems to play an important role in activation of ERK1/2 in SCs and in SC survival and proliferation. In addition, extracellular Ca2+ levels are also required for m-calpain activation and up-regulation in axons. Thus, regulation of Ca2+ levels is likely to be a useful method to promote SC proliferation. | Lisa B. Martensson Charlotta Lindwall Blom Lars B. Dahlin | 2017 | Neural Regeneration Research2017,12,4: | 4 |
| 3 | Development and tes- ting of the European Heart Failure Self- care Behaviour Scale显示文摘 | Jaarsma T Stromberg A Martensson J | 2003 | Eur J Heart Fail2003,5,3: | 1 |
| 4 | Effects of calcitonin gene-related peptide(CGRP) on islet hormone secretion in the pig显示文摘 | Martensson H Nobin A | 1987 | Diabetologia1987,30,3: | 1 |
| 5 | Impact of sepsis on levels of plasma cystatin C in AKI and non - AKI patients 显示文摘 | Martensson J Martling CR Oldner A | 2012 | Neph- ml Dial Transplant2012,27,2: | 1 |
| 6 | Steam pretreatment of H2SO4 impregnated Salix for the production of bioethanol 显示文摘 | Sassner P Martensson C G Galbe M | 2008 | Bioresouce technology2008,99,1: | 1 |
| 7 | Stability of CDT in vivo and in vitro显示文摘 | Martensson O Brandt R | 1997 | Alcohol Alcohol1997,32,4: | 1 |
| 8 | Development and testing ofthe European Heart Failure Self-Care Behaviour Scale显示文摘 | Jaarsma T Stromberg A Martensson J | 2003 | Eur J Heart Fail2003,5,3: | 1 |
| 9 | Potential for improving pea production by co-inoculation with fluorescent Pseudomonas and Rhizobium 显示文摘 | Dileep K B Berggren I Martensson A M | 2001 | Plant and Soil2001,229,: | 1 |
| 10 | Dahlstrom uelal patients with heart failure in primary health care: effects of a nurse-ledintervention on health-related quality of life and depression 显示文摘 | Martensson J Stromberg A | 2005 | Eur J Heart Fail2005,7,: | 1 |
| 11 | CTCF functions as a critical regulator of cell-cycle arrest and death after ligation of the B cell receptor on immature B cells显示文摘 | Qi CF Martensson A Mattioli M | 2003 | Proc Natl Acad Sci USA2003,100,2: | 1 |
| 12 | The European heart failureself-care behaviour scale revised into a nine-item scale(EHFScB-9):areliable and valid international instrument显示文摘 | Jaarsma T Arestedt KF Martensson J | 2009 | Eur J Heart Fail2009,11,1: | 1 |
| 13 | Stress-strain relationship of drying wood ( 1 ) : Development of a constitutive model 显示文摘 | MARTENSSON A | 1997 | Holzforschung1997,51,: | 1 |
| 14 | Galanin of the homologous species inhibits insulin secretion in the rat and in the pig显示文摘 | Lindskog S Dunning BE Martensson H | 1990 | Acta Physiol Scand1990,139,4: | 1 |
| 15 | Conditions when minimum variance control is the optimal experiment for identifying a minimum variance control显示文摘 | Martensson J | 2011 | Automatica2011,47,3: | 1 |
| 16 | Complementary signaling through flt3 and Interleukin-7 receptorαls indispensable for fetal and adult B cell genesis显示文摘 | Sitnicka E Brakebusch C Inga-Lill Martensson IL | 2003 | J Exp Med2003,198,10: | 1 |
| 17 | Novel biomarkers of acute kidney injury and failure: clinical applicability 显示文摘 | Martensson J Martling CR Bell M | 2012 | Br J Anaesth2012,109,6: | 1 |
| 18 | Neutral core oligosaccharides of bovine submaxillary mucin : use of lead tetraacetate in the cold for establishing branch positions显示文摘 | Martensson S Levery SB Bendiak B | 1998 | Eur J Biochem1998,258,: | 1 |
| 19 | The rise and fall of NGAL in acute kidney injury显示文摘 | MARTENSSON J BELLOMO R | 2014 | Blood Purif2014,37,4: | 1 |
| 20 | SU11752 inhibits the DNA-dependent protein kinase and DNA double-strand break repair resulting in ionizing radiation sensitization显示文摘 | Ismail IH Martensson S Moshinsky D | | 0,,: | 1 |