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| 1 | Introduction of DT40 cells into chick embryos显示文摘To examine the transfection of exogenous genes into chick embryos, applying the characteristics of avian leukosis virus (ALV)-induced chicken B cell line DT40 to the production of chimeric birds. Methods: The DT40cells incorporated with exogenous gene (lacZ constructs encoding Escherichia coli β-galactosidase: β-gal) were introduced into chick embryos by the injection of cells into stage X blastoderm. Manipulated eggs were incubated for 3 (trial 1 ) or 6 (trial 2) days, and the expression of lacZ DNA was detected by a histochemical staining method of β-galactosidase and polymerase chain reaction (PCR) analysis. Results: The survival rates of the manipulated embryos incubated for 3 days (stage 18-20: trial 1) and 6 days (stage 28, 30: trial 2) were about 42% and 38%, respectively.The expression rates of the lacZ gene in the embryos in the trials 1 and 2 were about 60% and 23%, respectively, for the survived embryos. Conclusio: The rate of embryonic viability and expression rate of introduced genes were not so high, but it suggested the possibility of utilizing the DT40 cells as a vector for carrying exogenous genes into chick embryos. | Mariko Toba Fumio Ebara Hiroki Furuta Yuichi Matsushimal Yasuo Kitagawa Noboru Fujihara | 2001 | Asian Journal of Andrology2001，3，1： | 3 |
| 2 | Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4显示文摘 | Kato J Matsushime H Hiebert SW | 1993 | Genes Dev1993，7，3： | 1 |
| 3 | Colony-stimulating factor 1 regulates novel cyclin during the G1 phase of the cell cycle显示文摘 | Roussel NF Ashman RA | 1991 | Cell1991，65，： | 1 |
| 4 | Colonystimulating factor Ⅰ regulates novel cyclins during the G1 phase of the cell cycle显示文摘 | Matsushime H Roussel MF Ashmun RA | 1991 | Cell1991，65，： | 1 |
| 5 | A kinase family, human flt sequence is highly conserved inverte brates and located on human chromosome显示文摘 | MATSUSHIME H YOSHIDA M C METAL S K | 1987 | J pn J Caner Res(Gann)1987，78，13： | 1 |
| 6 | Comparison of electronic structure of LiInO2 with NaInO2 显示文摘 | KAWAKAMI S MATSUSHIM S SHISHIDO T | 2003 | Journal of Alloys and Compounds2003，359，12： | 1 |
| 7 | Colony- stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle显示文摘 | Matsushime H Roussel MF Ashmun RA | 1991 | Cell1991，65，4： | 1 |
| 8 | Corrigendum to “Lysophosphatidylcholine enhances glucose-dependent insulin secretion via an orphan G-protein-coupled receptor” [Biochem. Biophys. Res. Commun. 326 (2005) 744–751]显示文摘 | Takatoshi Soga Takahide Ohishi Tetsuo Matsui Tetsu Saito Mitsuyuki Matsumoto Jun Takasaki Shun-ichiro Matsumoto Masazumi Kamohara Hideki Hiyama Shigeru Yoshida Kazuhiro Momose Yoshitaka Ueda Hitoshi Matsushime Masato Kobori Kiyoshi Furuichi | 2005 | Biochemical and Biophysical Research Communications2005，，1： | 1 |
| 9 | Direct binding of cychn D to the retinoblastoma product ( pRb )and pRb phosphorylation by the cyclin D - dependent kinase (CDK4) 显示文摘 | Kato J Matsushime H Hiebert SW | 1993 | Genes Dev1993，7，3： | 1 |
| 10 | Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4显示文摘 | Kato J Matsushime H Hiebert SW | 1993 | Genes Dev1993，7，3： | 1 |
| 11 | Genomic organization, chromosomal localization, and independent expression of human cyclin D genes 显示文摘 | Inaba T Matsushime H Valentine M | 1992 | Genomics1992，13，3： | 1 |
| 12 | Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins显示文摘 | Matsushime H Ewen M E Strom D K | 1992 | Cell1992，71，2： | 1 |
| 13 | Direct binding of cyclin D &127;to the retinoblastoma gene product(pRb) and pRb phosphory- lation &127; by &127; the cyclin D-dependent kinase CDK4显示文摘 | Kato J Matsushime H Hiebert SW | 1993 | Genes Develop1993，7，： | 1 |
| 14 | Identification and properties of an atypical catalytic subunit for mammalian D type G1 phase Cyclins显示文摘 | Ewen ME Strom DK | 1992 | Cell1992，71，2： | 1 |
| 15 | Genomic organization, chromosomal localization, and independent expression of human Cyclin D genes显示文摘 | Iosiya T Matsushime H Valentine M | 1992 | Genomics1992，13，3： | 1 |
| 16 | D-type cyclin-dependent kinase activity in mammalian cells显示文摘 | Matsushime H Quelle DE Shurtleff SA | 1994 | Mol Cell Bio11994，14，： | 1 |
| 17 | Cloning and expression of murine interleukin-1 receptor antagonist in macrophages stimulated by Colonystimulating factor 1 显示文摘 | Matsushime H Roussel MF | 1991 | Blood1991，78，3： | 1 |
| 18 | Immunocyte Ca2+influx system mediated by LTRPC2显示文摘 | Sano Y Inamura K Miyake A Mochizuki S Yokoi H Matsushime H | 2001 | Science2001，293，5533： | 1 |
| 19 | D-type cyclin-dependent kinase activity in mammalian cells显示文摘 | Matsushime H Quelle DE Shurtleff SA | 1994 | Mol Cell Biol1994，14，3： | 1 |
| 20 | D - type eyclin -dependent kinase activity in mammalian cells 显示文摘 | Matsushime H Quelle DE Shurtleff SA | 1994 | Mol Cell Biol1994，14，3： | 1 |
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