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| 1 | In vitro effect of amoxicillin and clarithromycin on the 3' region of cagA gene in Helicobacter pylori isolates显示文摘AIM:To evaluate the in vitro effect of amoxicillin and clarithromycin on the cag pathogenicity island(cag PAI).METHODS:One hundred and forty-nine clinical isolates of Helicobacter pylori(H.pylori)cultured from gastric biopsies from 206 Colombian patients with dyspeptic symptoms from a high-risk area for gastric cancer were included as study material.Antimicrobial susceptibility was determined by the agar dilution method.Resistant isolates at baseline and in amoxicillin and clarithromycin serial dilutions were subjected to genotyping(cagA,vacA alleles s and m),Glu-Pro-Ile-Tyr-Ala(EPIYA)polymerase chain reaction and random amplified polymorphic DNA(RAPD).Images of the RAPD amplicons were analyzed by Gel-Pro Analyzer 4.5program.Cluster analyses was done using SPSS 15.0statistical package,where each of the fingerprint bands were denoted as variables.Dendrograms were designed by following Ward’s clustering method and the estimation of distances between each pair of H.pylori isolates was calculated with the squared Euclidean distance.RESULTS:Resistance rates were 4%for amoxicillin and 2.7%for clarithromycin with 2%double resistances.Genotyping evidenced a high prevalence of the genotype cagA-positive/vacA s1m1.The 3’region of cagA gene was successfully amplified in 92.3%(12/13)of the baseline resistant isolates and in 60%(36/60)of the resistant isolates growing in antibiotic dilutions.Upon observing the distribution of the number of EPIYA repetitions in each dilution with respect to baseline isolates,it was found that in 61.5%(8/13)of the baseline isolates,a change in the number of EPIYA repetitions lowered antibiotic pressure.The gain and loss of EPIYA motifs resulted in a diversity of H.pylori subclones after bacterial adjustment to changing conditions product of antibiotic pressure.RAPD PCR evidenced the close clonal relationship between baseline isolates and isolates growing in antibiotic dilutions.CONCLUSION:Antibiotic pressure does not induce loss of the cag pathogenicity island,but it can leadin most cases-to genetic rearrangements within the3’region cagA of the founding bacteria that can affect the level of tyrosine phosphorylation impacting on its cellular effects and lead to divergence of cagA-positive subclones. | Javier Andrés Bustamante-Rengifo Andrés Januer Matta Alvaro Pazos Luis Eduardo Bravo | 2013 | World Journal of Gastroenterology2013，19，36： | 5 |
| 2 | Punctual mutations in 23S rRNA gene of clarithromycinresistant Helicobacter pylori in Colombian populations显示文摘AIM To characterize punctual mutations in 23S rRNA gene of clarithromycin-resistant Helicobacter pylori(H. pylori) and determine their association with therapeutic failure.METHODS PCR products of 23S rRNA gene V domain of 74 H. pylori isolates; 34 resistant to clarithromycin(29 from a low-risk gastric cancer(GC) population: TumacoColombia, and 5 from a high-risk population: TuquerresColombia) and 40 from a susceptible population(28 from Tumaco and 12 from Túquerres) were sequenced using capillary electrophoresis. The concordance between mutations of V domain 23S rRNA gene of H. pylori and therapeutic failure was determined using the Kappa coefficient and Mc Nemar's test was performed to determine the relationship between H. pylori mutationsand clarithromycin resistance.RESULTS23S rRNA gene from H. pylori was amplified in 56/74 isolates, of which 25 were resistant to clarithromycin(20 from Tumaco and 5 from Túquerres, respectively). In 17 resistant isolates(13 from Tumaco and 4 from Túquerres) the following mutations were found: A1593 T1, A1653 G2, C1770 T, C1954 T1, and G1827 C in isolates from Tumaco, and A2144 G from Túquerres. The mutations T2183 C, A2144 G and C2196 T in H. pylori isolates resistant to clarithromycin from Colombia are reported for the first time. No association between the H. pylori mutations and in vitro clarithromycin resistance was found. However, therapeutic failure of eradication treatment was associated with mutations of 23S rRNA gene in clarithromycin-resistant H. pylori(κ = 0.71).CONCLUSION The therapeutic failure of eradication treatment in the two populations from Colombia was associated with mutations of the 23S rRNA gene in clarithromycinresistant H. pylori. | Andrés Jenuer Matta Diana Carolina Zambrano Alvaro Jairo Pazos | 2018 | World Journal of Gastroenterology2018，24，14： | 5 |
| 3 | Starch Digestibility and Physicochemical and Cooking Properties of Irradiated Rice Grains显示文摘This study evaluated the starch digestibility, physicochemical properties and cooking characteristics of polished rice(varieties IRGA417 and IAC202) subjected to the doses of 0(as the control), 1, 2 and 5 k Gy of gamma radiation. The highest dose decreased the apparent amylose content, peak viscosity, water absorption and volume expansion. Irradiation increased the solid loss by 119% and 187% for IAC202 and IRGA417, respectively, when comparing the higher dose with the control. For IAC202, irradiation decreased the rapidly digestible starch and increased the slowly digestible starch(SDS) and resistant starch(RS). IRGA417 showed an elevation of SDS and a reduction in RS. And 1 k Gy dose of gamma radiation generated the highest level of RS for both the two varieties and presented the smallest changes in other physicochemical and cooking properties. | Luís Fernando POLESI Manoel Divino da MATTA JUNIOR Silene Bruder Silveira SARMENTO Solange Guidolin CANNIATTI-BRAZACA | 2017 | Rice science2017，24，1： | 3 |
| 4 | Genomic variability of Helicobacter pylori isolates of gastric regions from two Colombian populations显示文摘AIM To compare the genomic variability and the multiple colonization of Helicobacter pylori(H. pylori) in patients with chronic gastritis from two Colombian populations with contrast in the risk of developing gastric cancer(GC): Túquerres-Nari?o(High risk) and Tumaco-Nari?o(Low risk).METHODS Four hundred and nine patients from both genders with dyspeptic symptoms were studied. Seventy-two patients were included in whom H. pylori was isolated from three anatomic regions of the gastric mucosa,(31/206) of the high risk population of GC(Túquerres) and(41/203) of the low risk population of GC(Tumaco). The isolates were genotyped by PCR-RAPD. Genetic diversity between the isolates was evaluated by conglomerates analysis and multiple correspondence analyses. RESULTS The proportion of virulent genotypes of H. pylori was 99% in Túquerres and 94% in Tumaco. The coefficient of similarity of Nei-Li showed greater genetic diversityamong isolates of Túquerres(0.13) than those of Tumaco(0.07). After adjusting by age, gender and type of gastritis, the multiple colonization was 1.7 times more frequent in Túquerres than in Tumaco(P = 0.05).CONCLUSION In Túquerres, high risk of GC there was a greater probability of multiple colonization by H. pylori. From the analysis of the results of the PCR-RAPD, it was found higher genetic variability in the isolates of H. pylori in the population of high risk for the development of GC. | Andrés Jenuer Matta Alvaro Jairo Pazos Javier Andrés Bustamante-Rengifo Luis Eduardo Bravo | 2017 | World Journal of Gastroenterology2017，23，5： | 2 |
| 5 | Effect of sodium nitroprusside, a nitric oxide donor, on the in vitro maturation of bovine oocytes显示文摘 | Viana K S Caldas-Bussiere M C Matta S G C | 2006 | Anim Reprod Sci2006，10，： | 1 |
| 6 | Identification of differentially expressed genes in oral squamous cell carcinoma显示文摘 | Arora S Matta A Shukla NK | 2005 | Mol Carcinog2005，42，： | 1 |
| 7 | Photosynthetic performance of two coffee species under drought显示文摘 | Matta F M D Maestri M Barros R S | 1998 | Photosyuthetica1998，，2： | 1 |
| 8 | The Effect of Package Shape on Con-sumers’Judgments of Product Volume:Attention as a Men-tal Contaminant显示文摘 | Folkes V Matta S | 2004 | Journal of Consumer Research2004，31，2： | 1 |
| 9 | Cerebral pressure autoregulation and carbon dioxide reactivity during propofol-induced EEG suppression显示文摘 | Lam AM Strebel S | 1995 | Br J Anaesth1995，74，： | 1 |
| 10 | Do clinicians consider the results of the BinaxNOW Streptococcus pneumonia urinary antigen test when adapting antibiotic regimens for pneumonia patients显示文摘 | Matta M Kern6is S Day N | 2010 | Clin Microbiol Infect2010，16，9： | 1 |
| 11 | Two to twenty-year survivorship of the hip in 810 patients with operatively treated acetabular fractures显示文摘 | Tannast M Najibi S Matta JM | 2012 | J Bnne Joint Surg Am2012，94，17： | 1 |
| 12 | Over-expression of 14-3-3zeta is an early event in oral cancer显示文摘 | Matta A Bahadur S Duqqal R | 2007 | BMC Cancer2007，7，： | 1 |
| 13 | Right atrial lipoma显示文摘 | Matta R Neelakand hank S Sandhyamani S | 1996 | J Cardiovasc Surg1996，37，2： | 1 |
| 14 | Achieving perfect secrecy by pdf- bandlimited jamming显示文摘 | Marano S and Matta V | 2014 | IEEE Signal Processing Letters2014，21，1： | 1 |
| 15 | Fenton-like oxidation of 2,4,6-trinitrotoluene using different iron minerals显示文摘 | Matta R Hanna K Chiron S | 2007 | Science of The Total Environment2007，385，13： | 1 |
| 16 | Estimation of Target Lo- cation via Likelihood Approximation in Sensor Networks 显示文摘 | Addesso P Marano S Matta V | 2010 | IEEE Transactions on Signal Processing2010，58，3： | 1 |
| 17 | Extending Brand Equity : The Role of Goal Congruence 显示文摘 | Martin I Matta S Stewart D W | 2004 | Progress in Economics Research2004，8，： | 1 |
| 18 | The malleable brand: The role of implicit theories in evaluating brand extension显示文摘 | Yorkston E A Nunes J C Matta S | 2010 | Journal of Marketing: A Quarterly Publication of the American Marketing Association2010，74，1： | 1 |
| 19 | Fenton-like oxidation of 2,4,6-trinitrotoluene using different iron minerals显示文摘 | MATTA R HANNA K CHIRON S | 2007 | Science of the Total Environment2007，385，： | 1 |
| 20 | Cartilage-pefichondrium : an ideal graft mate- rial 显示文摘 | Chhapola S Matta I | 2012 | Indian J Otolaryngol Head Neck Surg2012，64，3： | 1 |
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