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| 1 | Exogenous cytokinin application to Actinidia chinensis var. deliciosa ‘Hayward’ fruit promotes fruit expansion through water uptake显示文摘Exogenous application of a cytokinin-like compound forchlorfenuron(CPPU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit quality.Actinidia chinensis var.deliciosa‘Hayward’fruit are very responsive to CPPU treatments,but the mechanism underlying the significant fruit weight increase and associated decrease in DM is unclear.In this study,we hypothesised that CPPU-enhanced growth increases fruit carbohydrate demand,but limited carbohydrate supply resulted in decreased fruit DM.During fruit development,CPPU effects on physical parameters,metabolites,osmotic pressure and transcriptional changes were assessed under conditions of both standard and a high carbohydrate supply.We showed that CPPU increased fruit fresh weight but the dramatic DM decrease was not carbohydrate limited.Enhanced glucose and fructose concentrations contributed to an increase in soluble carbohydrate osmotic pressure,which was correlated with increased water accumulation in CPPU-treated fruit and up-regulation of water channel aquaporin gene PIP2.4 at 49 days after anthesis.Transcipt analysis suggested that the molecular mechanism contributing to increased glucose and fructose concentrations was altered by carbohydrate supply.At standard carbohydrate supply,the early glucose increase in CPPU fruit was associated with reduced starch synthesis and increased starch degradation.When carbohydrate supply was high,the early glucose increase in CPPU fruit was associated with a general decrease in starch synthesis but up-regulation of vacuolar invertase and fructokinase genes.We conclude that CPPU affected fruit expansion by increasing the osmotically-driven water uptake and its effect was not carbohydrate supply-limited. | Simona Nardozza Helen L Boldingh Mark W Wohlers Andrew P Gleave Zhiwei Luo Guglielmo Costa Elspeth A MacRae Michael J Clearwater Annette C Richardson | 2017 | Horticulture Research2017，4，1： | 2 |
| 2 | Significance of the Properties of Materi- als in the Packing of Real Spherical Particles显示文摘 | Macrae J C Gray W A | 1961 | British Jour- nal of Applied Physics1961，12，4： | 1 |
| 3 | Carbohydrate metabolism during postharvest ripening in kiwifruit显示文摘 | MACRAE E QUICK W P BENKER C | 1992 | Planta1992，188，： | 1 |
| 4 | Carbohydrate metabolism during post-harvest ripening in kiwifruit显示文摘 | Macrae E Quik W P Benker | 1992 | planta1992，188，： | 1 |
| 5 | Biological activities of lignans显示文摘 | Macrae W D Towers G H N | 1984 | Phytochemistry1984，23，： | 1 |
| 6 | Uptake of mercury by thiol-grafted chitosan gel beads 显示文摘 | Merrifield J Davids W MacRae J | 2004 | Water Research2004，38，13： | 1 |
| 7 | Near-fault ground motion effects on simple structures显示文摘 | Macrae G A Morrow D V and Roeder C W | 2001 | Structure Engineering2001，127，9： | 1 |
| 8 | Effect of column stiffness on braced frame seismic behavior 显示文摘 | MacRae G A Kimura Y Roeder C W | 2004 | Journal of Strucral Engineering2004，130，3： | 1 |
| 9 | Development of a Positive Eu anomaly during diagenesis 显示文摘 | MacRae N D Nesbitt H W Kronberg B I | 1992 | Earth and Planetary Science Letters1992，109，34： | 1 |
| 10 | Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis显示文摘 | BULLEY S M RASSAM M HOSER D OTTO W SCHUNEMANN N WRIGHT M MACRAE E GLEAVE A LAING W | 2009 | Journal of Experimental Botany2009，60，3： | 1 |
| 11 | Develop ment of a positive Eu anomaly during diagenesis显示文摘 | MacRae N D Nesbill H W Kronberg B I | 1992 | Earth and Planetary Science Letters1992，109，34： | 1 |
| 12 | Carbohydrate metabolism during postharvest ripening in kiwifruit显示文摘 | Macrae E Quick W P Benker C | 1992 | Planta1992，188，： | 1 |
| 13 | Gene expression studies in kiwifruit and gene overexpression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis 显示文摘 | Bulley SM Rassam M Hoser D Otto W Schünemann N Wright M Macrae E Gleave A Laing W | 2009 | J Exp Bot2009，60，3： | 1 |
| 14 | Dynamic response and fatigue of steel tied-arch bridge显示文摘 | ROEDER C W MACRAE C CROCKER P | 2000 | Journal of Brdige Engineering2000，5，1： | 1 |
| 15 | Biological activities of lignans显示文摘 | Donald Macrae W Neil Towers G H | 1984 | Phytochemistry1984，23，： | 1 |
| 16 | Finding the self? An e- vent-related fMRI study显示文摘 | Kelley W M Macrae C N Wyland C L | 2002 | J Cogn Neurosci2002，14，5： | 1 |
| 17 | Ensilage char- acteristics of three tropical grasses as influenced by stage of growth and addition of molasses 显示文摘 | Tjandraatmadja M Norton B W MacRae I C | 1994 | World Journal of Micro- biology and Biotechnology1994，10，1： | 1 |
| 18 | Biological activities of lignans 显示文摘 | MACRAE W D TOWERS G H N | 1984 | Phytochemistry1984，23，6： | 1 |
| 19 | Chronic post-surgical pain: 10 years on 显示文摘 | Macrae W A | 2008 | Br J Anaesth2008，101，1： | 1 |
| 20 | Gene expression studies in kiwifruit and gene over- ex- pression in Arabidopsis indicates that GDP-L- galactose guanyltransferase is a major control point of vitamin C biosynthesis 显示文摘 | Bulley S M Rassam M Hoser D Otto W Schunemann N Wright M MacRae E Gleave A Laing W | 2009 | J Exp Bot2009，60，3： | 1 |
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