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| 1 | Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice显示文摘BACKGROUND Non-alcoholic fatty liver disease (NAFLD) has become an epidemic largely due to the worldwide increase in obesity. While lifestyle modifications and pharmacotherapies have been used to alleviate NAFLD, successful treatment options are limited. One of the main barriers to finding safe and effective drugs for long-term use in NAFLD is the fast initiation and progression of disease in the available preclinical models. Therefore, we are in need of preclinical models that (1) mimic the human manifestation of NAFLD and (2) have a longer progression time to allow for the design of superior treatments. AIM To characterize a model of prolonged high-fat diet (HFD) feeding for investigation of the long-term progression of NAFLD. METHODS In this study, we utilized prolonged HFD feeding to examine NAFLD features in C57BL/6 male mice. We fed mice with a HFD (60% fat, 20% protein, and 20% carbohydrate) for 80 wk to promote obesity (Old-HFD group, n = 18). A low-fat diet (LFD)(14% fat, 32% protein, and 54% carbohydrate) was administered for the same duration to age-matched mice (Old-LFD group, n = 15). An additional group of mice was maintained on the LFD (Young-LFD, n = 20) for a shorter duration (6 wk) to distinguish between age-dependent and age-independent effects. Liver, colon, adipose tissue, and feces were collected for histological and molecular assessments.RESULTS Prolonged HFD feeding led to obesity and insulin resistance. Histological analysis in the liver of HFD mice demonstrated steatosis, cell injury, portal and lobular inflammation and fibrosis. In addition, molecular analysis for markers of endoplasmic reticulum stress established that the liver tissue of HFD mice have increased phosphorylated Jnk and CHOP. Lastly, we evaluated the gut microbial composition of Old-LFD and Old-HFD. We observed that prolonged HFD feeding in mice increased the relative abundance of the Firmicutes phylum. At the genus level, we observed a significant increase in the abundance of Adercreutzia, Coprococcus, Dorea, and Ruminococcus and decreased relative abundance of Turicibacter and Anaeroplasma in HFD mice. CONCLUSION Overall, these data suggest that chronic HFD consumption in mice can mimic pathophysiological and some microbial events observed in NAFLD patients. | Kandy T Velázquez Reilly T Enos Jackie E Bader Alexander T Sougiannis Meredith S Carson Ioulia Chatzistamou James A Carson Prakash S Nagarkatti Mitzi Nagarkatti E Angela Murphy | 2019 | World Journal of Hepatology2019，11，8： | 6 |
| 2 | Risk factors for Barrett’s oesophagus and oesophageal adenocarcinoma:Results from the FINBAR study显示文摘AIM:To investigate risk factors associated with Barrett's oesophagus and oesophageal adenocarcinoma.METHODS:This all-Ireland population-based case-control study recruited 224 Barrett's oesophagus patients,227 oesophageal adenocarcinoma patients and 260 controls.All participants underwent a structured interview with information obtained about potential lifestyle and environmental risk factors.RESULTS:Gastro-oesophageal reflux was associated with Barrett's [OR 12.0(95% CI 7.64-18.7)] and oesophageal adenocarcinoma [OR 3.48(95% CI 2.25-5.41)].Oesophageal adenocarcinoma patients were more likely than controls to be ex-or current smokers [OR 1.72(95% CI 1.06-2.81)and OR 4.84(95% CI 2.72-8.61)respectively] and to have a high body mass index [OR 2.69(95% CI 1.62-4.46)].No significant associations were observed between these risk factors and Barrett's oesophagus.Fruit but not vegetables were negatively associated with oesophageal adenocarcinoma [OR 0.50(95% CI 0.30-0.86)].CONCLUSION:A high body mass index,a diet low in fruit and cigarette smoking may be involved in the progression from Barrett's oesophagus to oesophageal adenocarcinoma. | Lesley A Anderson RG Peter Watson Seamus J Murphy Brian T Johnston Harry Comber Jim Mc Guigan John V Reynolds Liam J Murray | 2007 | World Journal of Gastroenterology2007，13，10： | 5 |
| 3 | Have patients with esophagitis got an increased risk of adenocarcinoma? Results from a population-based study显示文摘AIM: To examine an increased risk of esophageal adenocarcinoma is restricted to patients who develop Barrett's esophagus or whether esophagitis per se is a risk factor for adenocarcinoma.METHODS: A population-based cohort of patients with histological evidence of esophagitis without Barrett's esophagus was constructed using electronic pathology reports relating to all esophageal biopsies in Northern Ireland between 1993 and 1996. Person-years of followup and incident cases of esophageal cancer were calculated by linking the cohort to death files and the Northern Ireland Cancer Registry records. Standardized incidence ratios (SIR) were calculated for esophageal cancers (adenocarcinoma, squamous cell carcinoma (SCC), and histologically unspecified cancers).RESULTS: A total of 2 013 patients in the cohort provided 13 559 patient-years of follow-up (mean follow-up 6.7 years). None of the patients developed adenocarcinoma. Three patients developed SCC, and six developed histologically unspecified cancers. The SIR for all esophageal cancers and for SCC were 2.73 (95%CI 1.25-5.19) and 2.93 (95%CI 0.61-8.59), respectively. In a sensitivity analysis in which all unspecified esophageal cancers were treated as adenocarcinomas, the SIR for adenocarcinoma was 2.64 (0.97-5.75).CONCLUSION: The risk of adenocarcinoma is not elevated in patients with histological evidence of esophagitis without Barrett's esophagus; however, these patients may have a moderately increased risk of SCC.Further studies are required to confirm these findings,which suggest that Barrett's esophagus, not esophagitis,is the key precursor lesion in the development of adenocarcinoma. | Seamus J Murphy Lesley A Anderson Brian T Johnston Deirdre A Fitzpatrick Peter RG Watson Pauline Monaghan Liam J Murray | 2005 | World Journal of Gastroenterology2005，11，46： | 4 |
| 4 | Competition for cytokines : T (reg) cells take all 显示文摘 | Scheffold A Murphy KM Hofer T | 2007 | Nat Immunol2007，8，： | 1 |
| 5 | Review of emerging issues in sediment treatment显示文摘 | Murphy T P Lawson A Kumagai M | 1999 | Aquatic Ecosystem Health and Management1999，2，： | 1 |
| 6 | Deciphering the biology of My cobacterium tuberculosis from the complete genome sequence 显示文摘 | Cole S T Brosch R Parkhill J Gamier T Churcher C Harris D Gordon S V Eiglmeier K Gas S Barry C E Tekaia F Badcock K Basham D Brown D Chillingworth T Cormor R Davies R Devlin K FeltweU T Gentles S Hamlin N Holroyd S Hornsby T Jagels K Kroghs A McLean J Moule S Murphy L Oliver K Osborne J Quail M A Rafandream M A Rogers J Rutter S Seeger K Skelton J Squaraes R Squares S Sulston J E Taylo K Whitehead S Barrell B G | 1998 | Nature1998，393，： | 1 |
| 7 | Complex dynamics and synchroniza tion of delayed-feedback nonlinear oscillators显示文摘 | Murphy T E Cohen A B Ravoori B | 2010 | Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences2010，368，1911： | 1 |
| 8 | Using a coupled eco-hydrodynamic model to predict habitat for target species following dam removal显示文摘 | Tomsic C A Granata T C Murphy R P | 2007 | Journal of Ecological Engineering2007，30，： | 1 |
| 9 | Biochemical characterization of the acquired metallo-beta-lactamase SPM-1 from Pseudomonas aeruginosa显示文摘 | MURPHY T A SIMM A M TOLEMAN M A | 2003 | Antimicrob Agents Chemother2003，47，2： | 1 |
| 10 | Persistent - colonization by Haemophilus influenzae in chronic obstructive pulmonary disease显示文摘 | Murphy T F Brauer A L Schiffmacher A T | 2004 | Am J Respir Crit Care Med2004，170，： | 1 |
| 11 | 2002, Evolutionary re-organization of a large operon in adzuki bean chloroplast DNA caused by inverted repeat movement显示文摘 | Perry A S Brennan S Murphy D J Kavanagh T A Wolfe K H | 2002 | DNA Research2002，9，5： | 1 |
| 12 | Sharing visual features for muhiclass and multiview object detection显示文摘 | Torralba A Murphy K P Freeman W T | 2007 | IEEE Trans on Pattern Analysis and Machine Intelligence2007，19，5： | 1 |
| 13 | Charac- terization of poly (3,3-bisethoxymethyl oxetane) and poly(3,3-bisazido methyl oxetane) and their block co- polymers显示文摘 | Murphy E A Ntozakhe T Murphy C J | 1989 | J ApplPolymSci1989，37，： | 1 |
| 14 | Biochemical characterization of the acquired metallo-13-1aetamase SPM-1 from Pseudomonas aeruginosa 显示文摘 | Murphy T A Simm A M Toleman M A | 2003 | Antimicrob Agents Chemother2003，47，2： | 1 |
| 15 | Development of a geometrycompensated neutron time-of-flight detector for ICF applications with approximately 200 ps time response显示文摘 | MURPHY T J LERCHE R A | | 0，，10： | 1 |
| 16 | Competition for cytokines: T(reg) cells take all显示文摘 | SCHEFFOLD A MURPHY KM HOFER T | 2007 | Nature Immunology2007，8，12： | 1 |
| 17 | Recombinant phycobiliproteins-Recombinant C-phycocyanins equipped with affinity tags, oligomerization, and biospecific recongnition domains显示文摘 | Cai Y A Murphy J T Wedemayer G J | 2001 | Anal Biochem2001，290，： | 1 |
| 18 | Abnormal postnatal maintenance of elevated DLK1 transcript levels in callipyge sheep显示文摘 | Murphy S K Freking B A Smith T P | 2005 | Mamm Cenome2005，16，3： | 1 |
| 19 | The timing of rotator cuff repair for the restoration of function显示文摘 | PETERSEN S A MURPHY T P | | J Shoulde0，，： | 1 |
| 20 | Anisntmpic met- al nanoparticles : ? synthesis, assembly, and optical applica- tions 显示文摘 | MURPHY C J SAU T K GOLE A M | 2005 | The Journal of Physical Chemistry B2005，109，13： | 1 |
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