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| 1 | Labeling of influenza viruses with synthetic fluorescent and biotin-labeled lipids显示文摘Direct labeling of virus particles is a powerful tool for the visualization of virus–cell interaction events. However, this technique involves the chemical modification of viral proteins that affects viral biological properties. Here we describe an alternative approach of influenza virus labeling that utilizes Function-Spacer-Lipid(FSL) constructs that can be gently inserted into the virus membrane. We assessed whether labeling with fluorescent(fluo-Ad-DOPE) or biotin-labeled(biot-CMG2-DOPE) probes has any deleterious effect on influenza virus hemagglutinin(HA) receptor specificity, neuraminidase(NA) activity, or replicative ability in vitro. Our data clearly show that neither construct significantly affected influenza virus infectivity or viral affinity to sialyl receptors. Neither construct influenced the NA activities of the influenza viruses tested, except the A/Puerto Rico/8/34(H1N1) strain. Our data indicate that lipid labeling provides a powerful tool to analyze influenza virus infection in vitro. | Natalia A Ilyushina Evgeny S Chernyy Elena Y Korchagina Aleksra S Gambaryan Stephen M Henry Nicolai V Bovin | 2014 | Virologica Sinica2014，29，4： | 2 |
| 2 | Hot water aging of polycarbonate 显示文摘 | Narkis M Nicolais L Apieella A | 1984 | Polymer Engineering and Science1984，24，3： | 1 |
| 3 | Systematic review of the application of quality improvement methodologies from the manufacturing industry to surgical healthcare显示文摘 | Nicolay C R Purkayastha S Greenhalgh A | 2012 | Br J Surg2012，99，3： | 1 |
| 4 | 欧洲议会和(欧盟)理事会2004年10月27日关于负责执行消费者保护法的国内机构相互合作的第2006/2004号条例(消费者保护合作条例)显示文摘序言欧洲议会与欧盟理事会,考虑到《建立欧洲共同体的条约》,尤其是第95条,考虑到委员会的建议,考虑到欧洲经济与社会委员会的意见①,经过与区域委员会的协商,根据《建立欧洲共同体的条约》第251条规定之程序①。 | 杜志华(译) 陆寰(译) J.P.BORRELL FONTELLES A Nicolai | 2010 | 中国国际私法与比较法年刊2010，13，1： | 1 |
| 5 | Recent results from experimental studies on laser–plasma coupling in a shock ignition relevant regime显示文摘 | P Koester L Antonelli S Atzeni J Badziak F Baffigi D Batani C A Cecchetti T Chodukowski F Consoli G Cristoforetti R De Angelis G Folpini L A Gizzi Z Kalinowska E Krousky M Kucharik L Labate T Levato R Liska G Malka Y Maheut A Marocchino P Nicolai T O’Dell | 2013 | Plasma Physics and Controlled Fusion2013，，12： | 1 |
| 6 | Large striatocapsular infarcts:clinical features and risk factors显示文摘 | Nicolai A Lazzarino LG Biasutti E | | 0，，： | 1 |
| 7 | Microalgae as human food: chemical and nutritional characteristics of the thermo-acidophilic mieroalga Galdieria sulphuraria显示文摘 | GRAZIANI G SCHIAVO S NICOLAI M A | 2013 | Food Function2013，4，： | 1 |
| 8 | Systematic review of the applica- tion of quality improvement methodologies from the manufacurlng industry to surgi- cal healtheare显示文摘 | Nicolay CR Purkayastha S Grenhalgh A | 2012 | Br J Surg2012，99，3： | 1 |
| 9 | Successful coronary revascular- ization improves prognosis in patients with previous myocardial infarction and evidence of viable myocardium at thallium-201 imaging显示文摘 | Cuoeolo A Petretta M Nicolai E | 1998 | Eur J Nucl Med1998，25，1： | 1 |
| 10 | Real time monitoring of cure and gelification of a thermoset matrix显示文摘 | Antonucci V Giordano M Cusano A Nasser J Nicolais L | 2006 | Composites Science and Technology2006，66，16： | 1 |
| 11 | Simultaneous analysis of ketamine and bupivacaine in plasma by high-performance liquid chromatography显示文摘 | Gross AS Nicolay A | 1999 | Journal of Chromatography B1999，728，： | 1 |
| 12 | RF identification in the use of belt RIP detection 显示文摘 | Nicolay T Treib A Alfons B | 2004 | Proceedings of IEEE Sensors2004，，： | 1 |
| 13 | Determination of the diffusion coeffi-cient of tissue,cuticle,cutin and wax of apple显示文摘 | VERAVERBEKE E A VERBOVEN P SCHEERLINCK N HOANG M H NICOLAI B M | 2003 | Journal of Food En-gineering2003，58，3： | 1 |
| 14 | Non-destructive measurement of bitter pit in apple fruit using NIR hyperspectral imaging 显示文摘 | Nicolai B M Lotze E Peirs A | 2006 | Postharvest Biology and Technology2006，40，1： | 1 |
| 15 | Assessment of systolic wall thickening using technetium-99mmethoxyisobutyli sonitrile in patients with coronary artery disease relation to thallium-201 scintigraphy with re-injection显示文摘 | Nicolai E Cuoclol A Pace L | 1995 | Eur J Nucl Med1995，22，： | 1 |
| 16 | CHAMPS-Muhizone-- A combined heat, air, moisture and pollutant simulation environment for whole-building performance analysis 显示文摘 | Feng W Grunewald J Nicolai A | 2011 | HVAC&R Research2011，18，： | 1 |
| 17 | Phenanthrene metabolism by Pseudomonas and Burkholderia strains显示文摘 | Natalia V Balashova Irina A Koshelava Nicolai P | 1999 | Process Biochemistry1999，35，： | 1 |
| 18 | Influence of harvest time and I-MCPapplication on postharvest ripening and ethylene biosynthesis of1Jonagold5 apple显示文摘 | BULENS I VANDEPOEL B HERTOG M PROFT M GEER-AERD A H NICOLAI B M | 2012 | Postharvest Biology and Technology2012，72，： | 1 |
| 19 | Systematic review of the application of quality improvement methodologies from the manufacturing industry to surgical healthcare显示文摘 | Nicolay CR Purkayastha S Greenhalgh A | 2012 | British Journal of Surgery2012，99，3： | 1 |
| 20 | The use of Lean and Six Sigma methodologies in surgery: a systematic review显示文摘 | Mason SE Nicolay CR Darzi A | 2015 | Surgeon2015，13，2： | 1 |
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