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| 1 | Methods for the extraction and RNA profiling of exosomes显示文摘AIM:To develop protocols for isolation of exosomes and characterization of their RNA content.METHODS:Exosomes were extracted from He La cell culture media and human blood serum using the Total exosome isolation(from cell culture media)reagent,and Total exosome isolation(from serum)reagent respectively.Identity and purity of the exosomes was confirmed by Nanosight?analysis,electron microscopy,and Western blots for CD63 marker.Exosomal RNA cargo was recovered with the Total exosome RNA and protein isolation kit.Finally,RNA was profiled using Bioanalyzer and quantitative reverse transcriptionpolymerase chain reaction(q RT-PCR)methodology.RESULTS:Here we describe a novel approach for robust and scalable isolation of exosomes from cell culture media and serum,with subsequent isolation and analysis of RNA residing within these vesicles.The isolation procedure is completed in a fraction of the time,compared to the current standard protocols utilizing ultracentrifugation,and allows to recover fully intact exosomes in higher yields.Exosomes were found tocontain a very diverse RNA cargo,primarily short sequences 20-200 nt(such as mi RNA and fragments of m RNA),however longer RNA species were detected as well,including full-length 18S and 28S r RNA.CONCLUSION:We have successfully developed a set of reagents and a workflow allowing fast and efficient extraction of exosomes,followed by isolation of RNA and its analysis by q RT-PCR and other techniques. | Emily Zeringer Mu Li Tim Barta Jeoffrey Schageman Ketil Winther Pedersen Axl Neurauter Susan Magdaleno Robert Setterquist Alexander V Vlassov | 2013 | World Journal of Methodology2013，3，1： | 5 |
| 2 | Interferon-gam- ma-induced conversion of tryptophan : immunologic and neuropsychiat- ric aspects显示文摘 | Wirleitner B Neurauter G Schrocksnadel K | 2003 | Curt Med Chem2003，10，16： | 1 |
| 3 | A compar- ison of the combination of epinephrine and vasopressin with lipid emulsion in a porcine model of asphyxial cardiac arrest after intravenous injection of bupivacaine显示文摘 | Mayr VD Mitterschiffthaler L Neurauter A | 2008 | Anesth Analg2008，106，5： | 1 |
| 4 | Chronic im- mune stimulation correlates with reduced phenylalanine turnover 显示文摘 | Neurauter G Schrocksnadel K Scholl-Burgi S | 2008 | Curr Drug Metab2008，9，7： | 1 |
| 5 | Interferon- gamma- induced Conversion of tryptophan : immunologic and neuro- psychiatric aspects显示文摘 | Wirleitner B Neurauter G Schrocksnadel K | 2003 | CUlT Med Chem2003，10，16： | 1 |
| 6 | A comparison of the combination of epinephrine and vasopressin with lipid emulsion in a porcine model of asphyxial cardiac arrest after intravenous injectionof bupivacaine 显示文摘 | Mayr V D Mitterschiffthaler L Neurauter A | 2008 | Anesth Analg2008，106，5： | 1 |
| 7 | More rapid method for simultaneous measurement of tryptophan and kynurenine by HPLC 显示文摘 | Laich A Neurauter G Widner B | 2002 | Clin Chem2002，48，3： | 1 |
| 8 | Down-regulatory effect of N-chlorotaurine on tryptophan degradation and neopterin production in human PBMC显示文摘 | Wirleitner B Neurauter G Nagl M | 2004 | Immunol Lett2004，93，2： | 1 |
| 9 | Interferonalpha-induced changes in tryptophan metabolism: Relationship to depression and paroxetine treatment 显示文摘 | Capuron L Neurauter G Musselman D L | 2003 | Biol Psychiatry2003，54，9： | 1 |
| 10 | Rapid measurement of total plasma homocysteine by HPLC 显示文摘 | Frick B Sehroeksnadel K Neurauter G ef al | 2003 | Clin Chim Acta2003，331，12： | 1 |
| 11 | A comparison of the combination of epinephrine and vasopressin with lipid emulsion in a porcine model of asphyxial cardiac arrest after intravenous injection of bupivacaine显示文摘 | Mayr VD Mitterschiffthaler L Neurauter A | 2008 | Anesth Analg2008，106，： | 1 |
| 12 | Differential effect of type I and type II interferons on neoptefin production and amino acid metabolism in human astroeyte-derived cells显示文摘 | Cano OD Neurauter G Fuehs D | 2008 | Neurosci Lett2008，438，1： | 1 |
| 13 | Effects of stomach in-flation on haemodynamic and pulmonary function during spontaneous circulation in pigs显示文摘 | Paal P Neurauter A Loedl M | 2009 | Resuscitation2009，80，4： | 1 |
| 14 | A comparison of the combination of epinephrine and vasopressin with lipid emulsion in a porcine model of asphyxial cardiac arrest after intravenous injection of bupivacaine显示文摘 | Mayr VD Mitterschiffthaler L Neurauter A | 2008 | Anesth Analg2008，106，5： | 1 |
| 15 | Three generations of mud volcanoes on the Louisiana continental slope显示文摘 | Neurauter T W Roberts H H | 1994 | Geo-Marine Letters1994，，14： | 1 |
| 16 | A co- mparison of the combination of epinephrine and vasopres-sin with lipid emulsion in a porcine model of asphyxial cardiac arrest after intravenous injection of bupivacaine 显示文摘 | Mayr VD Mitterschiftkhaler L Neurauter A | 2008 | AnesthAnalg2008，106，5： | 1 |
| 17 | A comparison of the combination of epinephrine and vasopressin with lipid emulsion in a porcine model of asphyxial cardiac arrest after intravenous injection of bupivacaine显示文摘 | Mayr VD Mitterschiffthaler L Neurauter A | 2008 | Anesth Analg2008，106，5： | 1 |
| 18 | Enhanced enzymatic degradation of tryptophan by indoleamine 2,3-dioxygenase contributes to the tryptophan-deficient state seen after major trauma 显示文摘 | Pellegrin K Neurauter G Wirleitner B | 2005 | Shock2005，23，： | 1 |
| 19 | Cell isolation and expansion using Dynabeads 显示文摘 | Neurauter AA Bonyhadi M Lien E | 2007 | Adv Biochem Eng Biotechnol2007，106，： | 1 |
| 20 | The Effects ofMultiple Infusion Line Extensions on Occlusion AlarmFunction of an Infusion Pump显示文摘 | Deckert D Buerkle C Neurauter A | 2009 | Anesth Analg2009，108，2： | 1 |
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