|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Evaluation of the AGCU Expressmarker 16 and 22 PCR Amplification Kits Using Biological Samples Applied to FTA Micro Cards in Reduced Volume Direct PCR Amplification Reactions显示文摘This study evaluated the performance of the Wuxi AGCU ScienTech Incorporation(HuiShan,Wuxi,China)AGCU Expressmarker 16(EX 16)and 22(EX22)short tandem repeat(STR)amplification kits in reduced reaction volumes using direct polymerase chain reaction(PCR)amplification workflows.The commercially available PowerPlex21(PP21)System(Promega,Wisconsin,USA),which follows similar direct workflows,was used as a reference.Anticoagulate blood applied to chemically impregnated FTATM Micro Cards(GE Healthcare UK Limited,Amersham Place,Little Chalfont,Buckinghamshire,HP79NA,UK)was used to represent a complex biological sample.Allelic concordance,first‑pass success rate,average peak heights,heterozygous peak height ratios(HPHRs),and intracolor and intercolor peak height balance were determined.In reduced volume PCR reactions,the performances of both the EX16 and EX22 STR amplification kits were comparable to that of the PP21 System.The level of performance was maintained at PCR reaction volumes,which are 40%of that recommended.The EX22 and PP21 System kits possess comparable overlapping genome coverage.This study evaluated the performance of the AGCU EX16 and EX22 STR amplification kits in reduced PCR reaction volumes using direct workflows in combination with whole blood applied to FTATM Micro Cards.Allelic concordance,first‑pass success rate,average peak heights,HPHRs,and intracolor and intercolor peak height balance were determined.A concordance analysis was completed that compared the performance of the EX16 and EX22 kits using human blood applied to FTA Micro Cards in combination with full,half,and reduced PCR reaction volumes.The PP21 System(Promega)was used as a reference kit.Where appropriate,the distributions of data were assessed using the Shapiro‑Wilk test.For normally‑distributed data,statistics were calculated using analysis of variance(ANOVA)and for nonparametric data the Wilcoxon/Kruskal‑Wallis test was used.Statistical significance was set at P<0.05.Confidence intervals for mean values were set at 95%.On using reduced volume PCR reactions in combination with dried blood spots applied to FTA sample collection cards,both the EX16 and EX22 kits were shown to generate STR profiles of sufficient quality to allow entry into National DNA databases.The performance of both EX16 and EX22 was comparable to that of the PP21 System.This study demonstrates the successful use of the Wuxi AGCU ScienTech Incorporation EX16 and EX22 kits in reduced PCR reaction volumes with complex biological samples applied to chemically impregnated FTA sample collection cards. | Samantha J Ogden Kathryn L Lamerton Peter J Tatnell | 2015 | Journal of Forensic Science and Medicine2015，1，1： | 2 |
| 2 | Control of Listeria monocytogenes in salmon;antimicrobial effect of salting,smoking and specific smoke compounds显示文摘 | NIEDZIELA J MACRAE M OGDEN I | 1998 | Lebensmittel Wissenschaft Technologie1998，31，2： | 1 |
| 3 | Tension band wiring ofdisplaced tibial tuberosity fractures in adolescents显示文摘 | Polakoff D R Bucholz R W Ogden J A | 1986 | CIin OrthpopRelat Res1986，209，209： | 1 |
| 4 | Total system conceptual ecological model显示文摘 | OGDEN J C DAVIS S M BARNES T K | 2005 | Wetlands2005，25，4： | 1 |
| 5 | Soil Organic Matter in a Ponderosa Pine Forest with Varying Seasons and Intervals of Prescribed Bum 显示文摘 | HATTEN J A ZABOWSKI D OGDEN A | 2008 | Forest Ecology and Management2008，255，7： | 1 |
| 6 | The use of conceptualecological models to guide ecosystem restoration in South Florida显示文摘 | OGDEN J C DAVIS S M JACOBS K J | 2005 | Wetlands2005，25，4： | 1 |
| 7 | Everglades ridge and slough conceptual ecological model显示文摘 | OGDEN J C | 2005 | Wetlands2005，25，4： | 1 |
| 8 | Infrared spectra and structures of matrix-isolated disilicon,digermanium,and ditin oxides (Si2O2,Ge2O2,and Sn2O2)显示文摘 | Anderson J S Ogden J S Rics M J | 1968 | Chem Commun1968，24，： | 1 |
| 9 | Exploring the impact of culture and acculturation on consumer purchase decisions:toward a micro-cultural perspective显示文摘 | Ogden D T Ogden J R Schau H J | 2004 | Academy of Marketing Science Review2004，3，： | 1 |
| 10 | Use of Blueberry to Study Genetic Control of Chilling Requirement and Cold Hardiness in Woody Perennials显示文摘 | Rowland L J Ogden E L Arora R | 1999 | Hort Sci1999，34，7： | 1 |
| 11 | Evaluation of Midwinter Cold Hardiness among 25 Rabbiteye blueberry c ultivar显示文摘 | Ehlenfeldt M K Ogden E L Rowland L J | 2006 | Hort Sci2006，41，3： | 1 |
| 12 | Enhanced lipid stability in whole wheat flour by lipase inactivation and antioxidant retention显示文摘 | ROSE D J OGDEN L V DUNN M L | 2008 | Cereal Chemistry2008，85，2： | 1 |
| 13 | Role of larger herbivores in seagrass communities显示文摘 | THAYER G W BJORNDAL K A OGDEN J C | 1984 | Estuaries1984，7，4： | 1 |
| 14 | Risk factors for congestive heart failure in US men and women:NHANES I epidemiologic follow-up study显示文摘 | He J Ogden LG Bazzano LA | 2001 | Arch Intern Med2001，161，7： | 1 |
| 15 | Cardiovascular respons eselicited by the“binge”administration of methamphetamine显示文摘 | Varner J Ogden BA Delcarpio J | 2002 | J Pharmacol Exp Ther2002，301，： | 1 |
| 16 | What's in a face? 'The role of doctor ethnicity, age and gender in the formation of patients' judgements:An experimental study 显示文摘 | Shaha R Ogden J | 2006 | Patient Education and Counseling2006，60，2： | 1 |
| 17 | Dietary sodium intake and incidence of congestive heart Failure in overweight US men and women: first national health and nutrition examination survey epidemilogic follow-up study显示文摘 | He J Ogden LG Bazzano LA | 2002 | Arch Intern Med2002，162，： | 1 |
| 18 | Chlorine and other constituents in relation to tobacco leaf- burn显示文摘 | Myhre D L Attoe O J Ogden W B | 1956 | Soil Science Society of Ameri-ca Journal1956，20，4： | 1 |
| 19 | 2000 CDC Growth Charts for the United States: methods and development显示文摘 | Kuczmarski R J Ogden C L Guo S S | 2002 | Vital Health Stat 112002，，246： | 1 |
| 20 | Use of a cytokine in conjugate for efficient shoot regeneration from leaf sections of high bush blueberry显示文摘 | Rowland L J Ogden E L | 1992 | HortScience1992，27，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员